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The map label for this gene is ddh [H]

Identifier: 237813572

GI number: 237813572

Start: 3233584

End: 3234588

Strand: Direct

Name: ddh [H]

Synonym: GBP346_A3348

Alternate gene names: 237813572

Gene position: 3233584-3234588 (Clockwise)

Preceding gene: 237813571

Following gene: 237813573

Centisome position: 78.9

GC content: 69.05

Gene sequence:

>1005_bases
GTGCGCGTGATTCTTTTCAGCAGCCGGCAGTACGACCACGATTCGTTCGATGCCGCCAACCAATCGTTCGGCTACCGGCT
GCACTTCCAGCCTTCCCATCTCGACGCGGAGACGGCGATCCTCGCGCGCGGCTATGAAGTCGTGTGCCCGTTCGTCAACG
ACACGCTCGACGCCGCCGTGCTCGAGACGCTCGCCGCGGGCGGCACGCGCGTCATCGCGCTGCGCTCGGCCGGCTTCAAC
CACGTCGATCTCGCGGCAGCCGCGCGGCTCGGGCTCACCGTCGTGCGCGTGCCCGCCTATTCGCCGCATGCGGTGGCCGA
GCACGCGGTCGCGCTGATCCTCGCGCTGAACCGGCGCCTGCCGCGCGCGGTCGCGCGCACCCGCGAAGGCGACTTCTCCC
TCAACGGGCTGCTCGGCTTCGATCTGTTCGGCAAGACGGTCGGCGTGATCGGCACGGGGCTCATCGGCAGCGTGTTCGCG
CGGATCATGACGGGCTTCGGGATGCGCGTGCTCGCACATTCGCTGCCGCCGCACGACGATGCGCTGATCGCGCTCGGCGT
GCGCTACGTGCCGCTCGACGCGCTGCTCGCCGAAGCGGACATCGTGAGCCTGCATTGCCCGCTGATGCCCGCGACCCATC
ACATGATCGACGCCGATGCGCTCGCGCGGATGAAGCCGGGCGCGATGCTGATCAACACGAGCCGCGGCGGCCTCGTCGAC
ACCCAGGCGCTGATCGACGCGCTGAAGACGGGCCAGCTCGGCCATCTCGGGCTCGACGTCTACGAAGAGGAAAGCGGGCT
GTTCTTCGAAGACCATTCGGACCGACCGCTGCAGGACGACGTGCTCGCGCGGCTGCTCACGTTTCCCAATGTGATCGTCA
CGTCGCACCAGGCGTTCTTCACGCGCGAGGCGCTCGCGGAGATCGCGCACACGACGCTGTCGAACATCCGCGCGTGGCAG
GACGGCGCGCCGCAGAACGTCGTCGACGCCGGGCGGCCCGGCTGA

Upstream 100 bases:

>100_bases
ATGCGCGAAGCCGGCACGGCGAAGCGCCCGGACGTCGTCACCGGGCTCCAGTGACGCCCGACTGAAGTAAACTGACGCGC
ACCTTTACCGGAGGCAAAGC

Downstream 100 bases:

>100_bases
CGGCGCGGCGGGCGACGAGCGGCGGCGGACACCGCGCGGTCGCGCGCGGCGCGCGGCGCGATTCGCGCCAAAGGCGCCGA
GGGGCGGATCGGCGAACGAT

Product: D-lactate dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRVILFSSRQYDHDSFDAANQSFGYRLHFQPSHLDAETAILARGYEVVCPFVNDTLDAAVLETLAAGGTRVIALRSAGFN
HVDLAAAARLGLTVVRVPAYSPHAVAEHAVALILALNRRLPRAVARTREGDFSLNGLLGFDLFGKTVGVIGTGLIGSVFA
RIMTGFGMRVLAHSLPPHDDALIALGVRYVPLDALLAEADIVSLHCPLMPATHHMIDADALARMKPGAMLINTSRGGLVD
TQALIDALKTGQLGHLGLDVYEEESGLFFEDHSDRPLQDDVLARLLTFPNVIVTSHQAFFTREALAEIAHTTLSNIRAWQ
DGAPQNVVDAGRPG

Sequences:

>Translated_334_residues
MRVILFSSRQYDHDSFDAANQSFGYRLHFQPSHLDAETAILARGYEVVCPFVNDTLDAAVLETLAAGGTRVIALRSAGFN
HVDLAAAARLGLTVVRVPAYSPHAVAEHAVALILALNRRLPRAVARTREGDFSLNGLLGFDLFGKTVGVIGTGLIGSVFA
RIMTGFGMRVLAHSLPPHDDALIALGVRYVPLDALLAEADIVSLHCPLMPATHHMIDADALARMKPGAMLINTSRGGLVD
TQALIDALKTGQLGHLGLDVYEEESGLFFEDHSDRPLQDDVLARLLTFPNVIVTSHQAFFTREALAEIAHTTLSNIRAWQ
DGAPQNVVDAGRPG
>Mature_334_residues
MRVILFSSRQYDHDSFDAANQSFGYRLHFQPSHLDAETAILARGYEVVCPFVNDTLDAAVLETLAAGGTRVIALRSAGFN
HVDLAAAARLGLTVVRVPAYSPHAVAEHAVALILALNRRLPRAVARTREGDFSLNGLLGFDLFGKTVGVIGTGLIGSVFA
RIMTGFGMRVLAHSLPPHDDALIALGVRYVPLDALLAEADIVSLHCPLMPATHHMIDADALARMKPGAMLINTSRGGLVD
TQALIDALKTGQLGHLGLDVYEEESGLFFEDHSDRPLQDDVLARLLTFPNVIVTSHQAFFTREALAEIAHTTLSNIRAWQ
DGAPQNVVDAGRPG

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=240, Percent_Identity=32.5, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI61743967, Length=271, Percent_Identity=35.0553505535055, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4557497, Length=271, Percent_Identity=35.0553505535055, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI145580578, Length=271, Percent_Identity=33.9483394833948, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4557499, Length=271, Percent_Identity=33.9483394833948, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI145580575, Length=271, Percent_Identity=33.9483394833948, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI6912396, Length=278, Percent_Identity=29.8561151079137, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1787645, Length=330, Percent_Identity=51.8181818181818, Blast_Score=340, Evalue=8e-95,
Organism=Escherichia coli, GI87082289, Length=307, Percent_Identity=31.2703583061889, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789279, Length=271, Percent_Identity=32.8413284132841, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788660, Length=216, Percent_Identity=31.9444444444444, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=266, Percent_Identity=28.1954887218045, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=260, Percent_Identity=27.6923076923077, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322116, Length=233, Percent_Identity=34.3347639484979, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320925, Length=288, Percent_Identity=31.9444444444444, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324055, Length=203, Percent_Identity=33.4975369458128, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324964, Length=206, Percent_Identity=28.1553398058252, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=128, Percent_Identity=31.25, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19921140, Length=296, Percent_Identity=30.0675675675676, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24646446, Length=261, Percent_Identity=34.4827586206897, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24646448, Length=261, Percent_Identity=34.4827586206897, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24646452, Length=261, Percent_Identity=34.4827586206897, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24646450, Length=261, Percent_Identity=34.4827586206897, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI62472511, Length=261, Percent_Identity=34.4827586206897, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28571528, Length=274, Percent_Identity=33.5766423357664, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45552429, Length=262, Percent_Identity=30.5343511450382, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28574286, Length=288, Percent_Identity=29.8611111111111, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585514, Length=270, Percent_Identity=30.3703703703704, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28574282, Length=270, Percent_Identity=30.3703703703704, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28574284, Length=270, Percent_Identity=30.3703703703704, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45551003, Length=270, Percent_Identity=30.3703703703704, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24585516, Length=273, Percent_Identity=24.9084249084249, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 35951; Mature: 35951

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILFSSRQYDHDSFDAANQSFGYRLHFQPSHLDAETAILARGYEVVCPFVNDTLDAAV
CEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCEEEECCCCCHHHHHH
LETLAAGGTRVIALRSAGFNHVDLAAAARLGLTVVRVPAYSPHAVAEHAVALILALNRRL
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
PRAVARTREGDFSLNGLLGFDLFGKTVGVIGTGLIGSVFARIMTGFGMRVLAHSLPPHDD
HHHHHHCCCCCCEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
ALIALGVRYVPLDALLAEADIVSLHCPLMPATHHMIDADALARMKPGAMLINTSRGGLVD
CEEEECCCCCCHHHHHHHCCEEEEECCCCCCHHHHCCHHHHHHCCCCEEEEECCCCCCCH
TQALIDALKTGQLGHLGLDVYEEESGLFFEDHSDRPLQDDVLARLLTFPNVIVTSHQAFF
HHHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCEEEECCHHHH
TREALAEIAHTTLSNIRAWQDGAPQNVVDAGRPG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVILFSSRQYDHDSFDAANQSFGYRLHFQPSHLDAETAILARGYEVVCPFVNDTLDAAV
CEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCEEEECCCCCHHHHHH
LETLAAGGTRVIALRSAGFNHVDLAAAARLGLTVVRVPAYSPHAVAEHAVALILALNRRL
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
PRAVARTREGDFSLNGLLGFDLFGKTVGVIGTGLIGSVFARIMTGFGMRVLAHSLPPHDD
HHHHHHCCCCCCEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
ALIALGVRYVPLDALLAEADIVSLHCPLMPATHHMIDADALARMKPGAMLINTSRGGLVD
CEEEECCCCCCHHHHHHHCCEEEEECCCCCCHHHHCCHHHHHHCCCCEEEEECCCCCCCH
TQALIDALKTGQLGHLGLDVYEEESGLFFEDHSDRPLQDDVLARLLTFPNVIVTSHQAFF
HHHHHHHHHCCCCCCCCCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCEEEECCHHHH
TREALAEIAHTTLSNIRAWQDGAPQNVVDAGRPG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8320209 [H]