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The map label for this gene is pur8 [H]

Identifier: 237813544

GI number: 237813544

Start: 3203231

End: 3204673

Strand: Direct

Name: pur8 [H]

Synonym: GBP346_A3320

Alternate gene names: 237813544

Gene position: 3203231-3204673 (Clockwise)

Preceding gene: 237813543

Following gene: 237813545

Centisome position: 78.15

GC content: 71.66

Gene sequence:

>1443_bases
ATGACCCACGGTCTGCACGGCCCCAAGCGCTGGTATGCGCTCGTCGTTCTCTGTCTGGGCGTGCTGATGATCGTGCTCGA
TTCGACGATCGTCAACGTCGCGCTGCCGTCGATCGGCGCGGACCTGCACTTCACCGAAACCGCGCTCGTCTGGATCGTGA
ACGCTTACATGCTGACGTTCGGCGGCTGCCTGCTGCTGGGCGGCCGGCTCGGCGATCTGTACGGCCAGCGCCGGATGTTC
CTCGCCGGCCTCACGCTGTTCACGCTCGCATCGCTCGCGTGCGGGCTCGCGCCAACGCAGTTCGTGCTGATCGCCGCGCG
CGCGGTGCAGGGGTTCGGTGGCGCGGTGGTGTCCGCCGTCGCGCTGTCGCTCATCATGAATCTCTTCACCGAGCCGGGCG
AGCGCGCGAAGGCGATGGGCGTCTACAGCTTCGTCTGCGCGGGCGGCGGCAGCCTCGGCGTACTGCTCGGCGGCGTCCTC
ACGAGCGTGCTCAGCTGGCACTGGATCTTCCTCGTCAATCTGCCGATCGGCGTCGCCGCGTACGCGCTGTCCGCCGCGCT
GCTGCCGAAGGTGCGCCCGCAGGCGGCCGACGCGCGGCTCGACGTCGCGGGCGCGATCGCCGTGACCGCTTCGCTGATGC
TCGCCGTCTACGGCATCGTCAACGGCAACGAGACCGGCTGGCTGTCGACGCAGACGGTCGCGACGCTCGCAGGCGCGGCC
GCGCTGCTCGCGGCGTTCATCGCGATCGAGACGCGCGCCGCGCATCCGCTGATGCCGCTCGCGCTCGCCACGCAGCGCAA
CGTCGCGGTCGCGAACGTGATCGGCGTGCTGTGGGCGGCCGCGATGTTCGCGTGGTTCTTTCTCTCCGCGCTCTACATGC
AGCGCGTGCTCGGCTACGGGCCGCTCCAGGTCGGCCTGGCGTTTCTGCCCGCGAACCTGATCATGGCCGCGTTCTCGCTC
GGCTTGTCCGCGAGGATCGTGATGCGCTTCGGCATCCGCCGCGCGATCGGCGCCGGGCTCGTCATCGCGGCCGCGGGCCT
TGCCCTCTTCGCGCGTGCACCCGCCGACGGCGGCTTCGTCGCGCACGTGCTGCCGGGGATGATACTCGTCGGCGTCGGCG
CGGGCGTCGCGTTCAATCCGGTGCTGCTCGCCGCGATGAGCGACGTCGCGCCGAGCGATTCGGGGCTTGCCTCGGGCATC
GTCAACACCTCGTTCATGATGGGCGGCGCCCTCGGCCTCGCGGTGCTCGCGAGCGTCGCGTCCGCGCGCACCGACGCGCT
CGCCGCCGCGCGCGCGGCGCTGCCCGTCGCGCTCAACGGCGGCTATCACGCGGCGTTCGCGTGCGGCGCGGCATTCGCGG
CGCTGGCCGCCGGCCTCGCGTACGCGCTGCGCATCCGCGCGCAATCGAACGCCGCGGCCGAACTGCACGGCGCCGCGCAC
TGA

Upstream 100 bases:

>100_bases
CCGCGGCCCGCACGCAAGCGAACGGCCTGTCAGCAACCGGCAGGAAGGCAACTCACGACACGCTTACAATGCTGCCTCCC
ACCCTTCGGAGGCATTCCCG

Downstream 100 bases:

>100_bases
GCGCGGGAAGACGGCGGCGCGCTGCCGTCAGCGCATCGCGCGCGTCCCGCCGCATCGTTGCGCGCCGCATCGGGCACGTT
CGCCTTCGATCGCCGGCAGT

Product: drug resistance MFS transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTHGLHGPKRWYALVVLCLGVLMIVLDSTIVNVALPSIGADLHFTETALVWIVNAYMLTFGGCLLLGGRLGDLYGQRRMF
LAGLTLFTLASLACGLAPTQFVLIAARAVQGFGGAVVSAVALSLIMNLFTEPGERAKAMGVYSFVCAGGGSLGVLLGGVL
TSVLSWHWIFLVNLPIGVAAYALSAALLPKVRPQAADARLDVAGAIAVTASLMLAVYGIVNGNETGWLSTQTVATLAGAA
ALLAAFIAIETRAAHPLMPLALATQRNVAVANVIGVLWAAAMFAWFFLSALYMQRVLGYGPLQVGLAFLPANLIMAAFSL
GLSARIVMRFGIRRAIGAGLVIAAAGLALFARAPADGGFVAHVLPGMILVGVGAGVAFNPVLLAAMSDVAPSDSGLASGI
VNTSFMMGGALGLAVLASVASARTDALAAARAALPVALNGGYHAAFACGAAFAALAAGLAYALRIRAQSNAAAELHGAAH

Sequences:

>Translated_480_residues
MTHGLHGPKRWYALVVLCLGVLMIVLDSTIVNVALPSIGADLHFTETALVWIVNAYMLTFGGCLLLGGRLGDLYGQRRMF
LAGLTLFTLASLACGLAPTQFVLIAARAVQGFGGAVVSAVALSLIMNLFTEPGERAKAMGVYSFVCAGGGSLGVLLGGVL
TSVLSWHWIFLVNLPIGVAAYALSAALLPKVRPQAADARLDVAGAIAVTASLMLAVYGIVNGNETGWLSTQTVATLAGAA
ALLAAFIAIETRAAHPLMPLALATQRNVAVANVIGVLWAAAMFAWFFLSALYMQRVLGYGPLQVGLAFLPANLIMAAFSL
GLSARIVMRFGIRRAIGAGLVIAAAGLALFARAPADGGFVAHVLPGMILVGVGAGVAFNPVLLAAMSDVAPSDSGLASGI
VNTSFMMGGALGLAVLASVASARTDALAAARAALPVALNGGYHAAFACGAAFAALAAGLAYALRIRAQSNAAAELHGAAH
>Mature_479_residues
THGLHGPKRWYALVVLCLGVLMIVLDSTIVNVALPSIGADLHFTETALVWIVNAYMLTFGGCLLLGGRLGDLYGQRRMFL
AGLTLFTLASLACGLAPTQFVLIAARAVQGFGGAVVSAVALSLIMNLFTEPGERAKAMGVYSFVCAGGGSLGVLLGGVLT
SVLSWHWIFLVNLPIGVAAYALSAALLPKVRPQAADARLDVAGAIAVTASLMLAVYGIVNGNETGWLSTQTVATLAGAAA
LLAAFIAIETRAAHPLMPLALATQRNVAVANVIGVLWAAAMFAWFFLSALYMQRVLGYGPLQVGLAFLPANLIMAAFSLG
LSARIVMRFGIRRAIGAGLVIAAAGLALFARAPADGGFVAHVLPGMILVGVGAGVAFNPVLLAAMSDVAPSDSGLASGIV
NTSFMMGGALGLAVLASVASARTDALAAARAALPVALNGGYHAAFACGAAFAALAAGLAYALRIRAQSNAAAELHGAAH

Specific function: May be involved in active puromycin efflux energized by a proton-dependent electrochemical gradient. In addition, it could be implicated in secreting N-acetylpuromycin, the last intermediate of the puromycin biosynthesis pathway, to the environment [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=423, Percent_Identity=31.6784869976359, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790195, Length=407, Percent_Identity=29.4840294840295, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789042, Length=420, Percent_Identity=26.4285714285714, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788710, Length=315, Percent_Identity=27.3015873015873, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788392, Length=358, Percent_Identity=29.8882681564246, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790146, Length=166, Percent_Identity=28.3132530120482, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=451, Percent_Identity=25.9423503325942, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319770, Length=397, Percent_Identity=24.6851385390428, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323520, Length=449, Percent_Identity=25.1670378619154, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=516, Percent_Identity=22.2868217054264, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=154, Percent_Identity=30.5194805194805, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48760; Mature: 48629

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHGLHGPKRWYALVVLCLGVLMIVLDSTIVNVALPSIGADLHFTETALVWIVNAYMLTF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHH
GGCLLLGGRLGDLYGQRRMFLAGLTLFTLASLACGLAPTQFVLIAARAVQGFGGAVVSAV
CCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHH
ALSLIMNLFTEPGERAKAMGVYSFVCAGGGSLGVLLGGVLTSVLSWHWIFLVNLPIGVAA
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCHHHHH
YALSAALLPKVRPQAADARLDVAGAIAVTASLMLAVYGIVNGNETGWLSTQTVATLAGAA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ALLAAFIAIETRAAHPLMPLALATQRNVAVANVIGVLWAAAMFAWFFLSALYMQRVLGYG
HHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLQVGLAFLPANLIMAAFSLGLSARIVMRFGIRRAIGAGLVIAAAGLALFARAPADGGFV
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AHVLPGMILVGVGAGVAFNPVLLAAMSDVAPSDSGLASGIVNTSFMMGGALGLAVLASVA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SARTDALAAARAALPVALNGGYHAAFACGAAFAALAAGLAYALRIRAQSNAAAELHGAAH
HHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCCCC
>Mature Secondary Structure 
THGLHGPKRWYALVVLCLGVLMIVLDSTIVNVALPSIGADLHFTETALVWIVNAYMLTF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHH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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7916693 [H]