Click here to switch to the map view.

The map label for this gene is pyrC' [H]

Identifier: 237813512

GI number: 237813512

Start: 3175885

End: 3177162

Strand: Reverse

Name: pyrC' [H]

Synonym: GBP346_A3288

Alternate gene names: 237813512

Gene position: 3177162-3175885 (Counterclockwise)

Preceding gene: 237813513

Following gene: 237813511

Centisome position: 77.52

GC content: 71.6

Gene sequence:

>1278_bases
ATGAAGATTCATATCAAAGGCGGCACGCTCATCGATCCGGTGGCCGGCACGCAGCGGCAGGCCGACGTGTTCGTCGCGGC
CGGCAAGGTGGCCGCGATCGGCGCGGCGCCGGCCGATTTCAACGCGGCGAAGACGATCGACGCGACGGGGCTGATCGTCG
CGCCGGGCTTCGTCGATTTGTCGGCGCGGCTGCGCGAGCCCGGCTACGAGCATAAGGCGACGCTCGAATCCGAGATGGCG
GCGGCGGTCGCGGGCGGCGTGACGAGCCTCGTGTGCCCGCCCGACACCGATCCGGTGCTCGACGAGCCGGGCCTCGTCGA
AATGCTGAAGTTTCGCGCCCGCAACCGGAATCAGGCGCACGTGTATCCGCTCGGCGCGCTGACGGTCGGCCTGAAAGGGC
AGGTCATCACCGAGATGGTCGAGCTGACCGAGGCGGGCTGCATCGGCTTCACGCAGGCGAACGTGCCCGTCACCGATACG
CAGGTGCTGCTGCGCGCGCTGCAGTACGCGAGCACCTACGGCTACACGGTGTGGCTGCGCCCGCTCGACGCGTTTCTCGC
GAAGGGCGGCGTCGCGGCGAGCGGGCCCGTCGCGTCGCGGCTCGGCCTGTCGGGCGTGCCGGTCGCGGCCGAGACGATCG
CGCTGCATACGCTGTTCGAGCTGATGCGGGTGACGGGCGCGCGCGTGCACGTCGCGCGGCTGTCGTCGGCGGCCGGCGTC
GCGCTCGTGCGCGCCGCGAAGGCCGAGGGCCTGCCCGTGACCTGCGATGTCGGCGCGAACCACCTGCATCTGATCGATGT
CGACATCGGCTACTTCGACGCGCAGTTCCGGCTCGATCCGCCGCTGCGCGCCGAGCGCGACCGCGAGGCGATTCGCGCGG
CGCTCGCCGACGGCACGATCGACGCGATCTGCTCGGATCACACGCCCGTCGATGACGACGAGAAGCTGCTGCCGTTCGCC
GAGGCGACGCCCGGCGCGACGGGCCTCGAGCTGCTGTTGTCGCTGACCGTGAAGTGGGCGCGCGAAGCGGGCGTGCCGCT
CGCGCGGGCGCTCGCGGCGATCACCTCGGCGCCCGCCGATGTGCTGAAGCTGCCCGCCGGCCGTATCGGCGAAGGCGCGC
CGGCCGACCTGTGCGTGTTCGATCCGAATGCGCACTGGCGCGTCGAGCCCCGCGCGCTGAAGAGCCAGGGCCACAACACG
CCGTTCCTCGGCTATGAGCTGCCGGCGCGAGTGTGCGCGACGCTCGTCGCGGGGCAGGTGGCGTTCGAGCGTCGCTGA

Upstream 100 bases:

>100_bases
AGCGACTGACGGGGCGCGGGCGTCGCGGGCAACGCGGCGAGCCCGCGCCGCGCGCCGGCTTCGCCGAAATCAACGCATTC
AACGCATCAACAGACAGCGC

Downstream 100 bases:

>100_bases
ACGAGGGCCGCATCATGAGAGCCCTACGCAAGGCGCGCCTCGTCCTGCATCTGCTGCGCGGCATGGCGACCGTCGCGCTG
CGCTTCGAGCGCGCGACGCC

Product: dihydroorotase

Products: Allantoate; H+ [C]

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKIHIKGGTLIDPVAGTQRQADVFVAAGKVAAIGAAPADFNAAKTIDATGLIVAPGFVDLSARLREPGYEHKATLESEMA
AAVAGGVTSLVCPPDTDPVLDEPGLVEMLKFRARNRNQAHVYPLGALTVGLKGQVITEMVELTEAGCIGFTQANVPVTDT
QVLLRALQYASTYGYTVWLRPLDAFLAKGGVAASGPVASRLGLSGVPVAAETIALHTLFELMRVTGARVHVARLSSAAGV
ALVRAAKAEGLPVTCDVGANHLHLIDVDIGYFDAQFRLDPPLRAERDREAIRAALADGTIDAICSDHTPVDDDEKLLPFA
EATPGATGLELLLSLTVKWAREAGVPLARALAAITSAPADVLKLPAGRIGEGAPADLCVFDPNAHWRVEPRALKSQGHNT
PFLGYELPARVCATLVAGQVAFERR

Sequences:

>Translated_425_residues
MKIHIKGGTLIDPVAGTQRQADVFVAAGKVAAIGAAPADFNAAKTIDATGLIVAPGFVDLSARLREPGYEHKATLESEMA
AAVAGGVTSLVCPPDTDPVLDEPGLVEMLKFRARNRNQAHVYPLGALTVGLKGQVITEMVELTEAGCIGFTQANVPVTDT
QVLLRALQYASTYGYTVWLRPLDAFLAKGGVAASGPVASRLGLSGVPVAAETIALHTLFELMRVTGARVHVARLSSAAGV
ALVRAAKAEGLPVTCDVGANHLHLIDVDIGYFDAQFRLDPPLRAERDREAIRAALADGTIDAICSDHTPVDDDEKLLPFA
EATPGATGLELLLSLTVKWAREAGVPLARALAAITSAPADVLKLPAGRIGEGAPADLCVFDPNAHWRVEPRALKSQGHNT
PFLGYELPARVCATLVAGQVAFERR
>Mature_425_residues
MKIHIKGGTLIDPVAGTQRQADVFVAAGKVAAIGAAPADFNAAKTIDATGLIVAPGFVDLSARLREPGYEHKATLESEMA
AAVAGGVTSLVCPPDTDPVLDEPGLVEMLKFRARNRNQAHVYPLGALTVGLKGQVITEMVELTEAGCIGFTQANVPVTDT
QVLLRALQYASTYGYTVWLRPLDAFLAKGGVAASGPVASRLGLSGVPVAAETIALHTLFELMRVTGARVHVARLSSAAGV
ALVRAAKAEGLPVTCDVGANHLHLIDVDIGYFDAQFRLDPPLRAERDREAIRAALADGTIDAICSDHTPVDDDEKLLPFA
EATPGATGLELLLSLTVKWAREAGVPLARALAAITSAPADVLKLPAGRIGEGAPADLCVFDPNAHWRVEPRALKSQGHNT
PFLGYELPARVCATLVAGQVAFERR

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=405, Percent_Identity=26.4197530864197, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786722, Length=456, Percent_Identity=28.9473684210526, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87082175, Length=441, Percent_Identity=24.2630385487528, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI193204318, Length=412, Percent_Identity=24.2718446601942, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322218, Length=392, Percent_Identity=25.2551020408163, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24642586, Length=394, Percent_Identity=25.1269035532995, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI18859883, Length=212, Percent_Identity=30.6603773584906, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.5 [C]

Molecular weight: Translated: 44582; Mature: 44582

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHIKGGTLIDPVAGTQRQADVFVAAGKVAAIGAAPADFNAAKTIDATGLIVAPGFVDL
CEEEECCCCEECCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCEECCCCEEEECCCEEH
SARLREPGYEHKATLESEMAAAVAGGVTSLVCPPDTDPVLDEPGLVEMLKFRARNRNQAH
HHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEE
VYPLGALTVGLKGQVITEMVELTEAGCIGFTQANVPVTDTQVLLRALQYASTYGYTVWLR
EEEEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEC
PLDAFLAKGGVAASGPVASRLGLSGVPVAAETIALHTLFELMRVTGARVHVARLSSAAGV
HHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCH
ALVRAAKAEGLPVTCDVGANHLHLIDVDIGYFDAQFRLDPPLRAERDREAIRAALADGTI
HHHHHHCCCCCEEEEECCCCEEEEEEEECCEEEEEEEECCCCCCHHHHHHHHHHHHCCCH
DAICSDHTPVDDDEKLLPFAEATPGATGLELLLSLTVKWAREAGVPLARALAAITSAPAD
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHH
VLKLPAGRIGEGAPADLCVFDPNAHWRVEPRALKSQGHNTPFLGYELPARVCATLVAGQV
HHHCCCCCCCCCCCCCEEEECCCCCEEECHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
AFERR
HHCCC
>Mature Secondary Structure
MKIHIKGGTLIDPVAGTQRQADVFVAAGKVAAIGAAPADFNAAKTIDATGLIVAPGFVDL
CEEEECCCCEECCCCCCCCCCEEEEECCCEEEEECCCCCCCCCCEECCCCEEEECCCEEH
SARLREPGYEHKATLESEMAAAVAGGVTSLVCPPDTDPVLDEPGLVEMLKFRARNRNQAH
HHHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEE
VYPLGALTVGLKGQVITEMVELTEAGCIGFTQANVPVTDTQVLLRALQYASTYGYTVWLR
EEEEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEC
PLDAFLAKGGVAASGPVASRLGLSGVPVAAETIALHTLFELMRVTGARVHVARLSSAAGV
HHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCH
ALVRAAKAEGLPVTCDVGANHLHLIDVDIGYFDAQFRLDPPLRAERDREAIRAALADGTI
HHHHHHCCCCCEEEEECCCCEEEEEEEECCEEEEEEEECCCCCCHHHHHHHHHHHHCCCH
DAICSDHTPVDDDEKLLPFAEATPGATGLELLLSLTVKWAREAGVPLARALAAITSAPAD
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHH
VLKLPAGRIGEGAPADLCVFDPNAHWRVEPRALKSQGHNTPFLGYELPARVCATLVAGQV
HHHCCCCCCCCCCCCCEEEECCCCCEEECHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
AFERR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Allantoin; H2O [C]

Specific reaction: Allantoin + H2O --> Allantoate + H+ [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; p-Chloromercuribenzene-sulphonic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]