Click here to switch to the map view.

The map label for this gene is rph [H]

Identifier: 237813382

GI number: 237813382

Start: 3056384

End: 3057115

Strand: Direct

Name: rph [H]

Synonym: GBP346_A3155

Alternate gene names: 237813382

Gene position: 3056384-3057115 (Clockwise)

Preceding gene: 237813376

Following gene: 237813383

Centisome position: 74.57

GC content: 70.36

Gene sequence:

>732_bases
ATGACGAATTCCTCCCTACGCCCGAGCGGGCGCCGCGCCGACCAGTTGCGCGACGTGCGCATCACGCGCCACTACACGAA
GCATGCGGAAGGCGCGGTGCTCGTCGAGTTCGGCGACACGAAAGTGATCTGCACGGCGAGCGTCGCCGAGCGCGTGCCCG
AATTCCTGCGCGAGCGCGGCCAAGGCTGGCTCACCGCCGAATACGGGATGCTGCCGCGTGCGACGCACACGCGCAGCGAC
CGCGAAGCGGCGCGCGGCAAGCAAACCGGCCGCACGCAGGAAATTCAGCGCCTGATCGGCCGCGCGCTGCGCGCGGTATT
CGATCTGAACGCACTCGGCCCACGCACGCTGCACCTCGATTGCGACGTGATCCAGGCCGACGGCGGCACGCGCACGGCGA
GCATCACGGGCGCGTTCGTCGCCGCGCACGACGCGGTGACGAAACTCGTCGCGGCCGGCAGGATCGCGCGCTCGCCGATC
ACCGATTACGTCGCGGCGATCTCGGTCGGCGTGTTCGGCGGCACGCCGGTGCTCGACCTCGACTACGGCGAAGACTCCGC
GTGCGACACCGACATGAACGTCGTGATGACGGGCGCGGGCGGCTTCGTCGAGGTGCAGGGCACCGCGGAAGGCGCGCCGT
TCTCGCGCACCGAGATGAACGCGCTTCTCGATCTCGCGCAGGCCGGCATCGGCGAGCTCGTGCGGCTGCAGCGCGCGGCG
CTGGAGGCATGA

Upstream 100 bases:

>100_bases
GCGCGAAACATCCATTATCCCATTTTTGCGGGCCGGACCCGCCGGGCCGATGCGCCGCGCGCGCGGTAAAATTCGGGTTT
CTCCTCAGCCTATTGTCCCG

Downstream 100 bases:

>100_bases
CGATGTCGCACGCATCGCCCGACGCCGCGCGCTCGCGCATCGTCCTCGCCTCGAACAATCCCGGCAAGCTGCGCGAGTTC
GCCGCGCTCTTCTCGACGGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTNSSLRPSGRRADQLRDVRITRHYTKHAEGAVLVEFGDTKVICTASVAERVPEFLRERGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLDCDVIQADGGTRTASITGAFVAAHDAVTKLVAAGRIARSPI
TDYVAAISVGVFGGTPVLDLDYGEDSACDTDMNVVMTGAGGFVEVQGTAEGAPFSRTEMNALLDLAQAGIGELVRLQRAA
LEA

Sequences:

>Translated_243_residues
MTNSSLRPSGRRADQLRDVRITRHYTKHAEGAVLVEFGDTKVICTASVAERVPEFLRERGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLDCDVIQADGGTRTASITGAFVAAHDAVTKLVAAGRIARSPI
TDYVAAISVGVFGGTPVLDLDYGEDSACDTDMNVVMTGAGGFVEVQGTAEGAPFSRTEMNALLDLAQAGIGELVRLQRAA
LEA
>Mature_242_residues
TNSSLRPSGRRADQLRDVRITRHYTKHAEGAVLVEFGDTKVICTASVAERVPEFLRERGQGWLTAEYGMLPRATHTRSDR
EAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLDCDVIQADGGTRTASITGAFVAAHDAVTKLVAAGRIARSPIT
DYVAAISVGVFGGTPVLDLDYGEDSACDTDMNVVMTGAGGFVEVQGTAEGAPFSRTEMNALLDLAQAGIGELVRLQRAAL
EA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=67.1428571428571, Blast_Score=290, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI71981632, Length=191, Percent_Identity=26.1780104712042, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25995; Mature: 25864

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSSLRPSGRRADQLRDVRITRHYTKHAEGAVLVEFGDTKVICTASVAERVPEFLRERG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHC
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLD
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
CDVIQADGGTRTASITGAFVAAHDAVTKLVAAGRIARSPITDYVAAISVGVFGGTPVLDL
EEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEE
DYGEDSACDTDMNVVMTGAGGFVEVQGTAEGAPFSRTEMNALLDLAQAGIGELVRLQRAA
ECCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEA
HCC
>Mature Secondary Structure 
TNSSLRPSGRRADQLRDVRITRHYTKHAEGAVLVEFGDTKVICTASVAERVPEFLRERG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHC
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLD
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
CDVIQADGGTRTASITGAFVAAHDAVTKLVAAGRIARSPITDYVAAISVGVFGGTPVLDL
EEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEE
DYGEDSACDTDMNVVMTGAGGFVEVQGTAEGAPFSRTEMNALLDLAQAGIGELVRLQRAA
ECCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA