Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 237813246

GI number: 237813246

Start: 2931202

End: 2932173

Strand: Reverse

Name: thyA [H]

Synonym: GBP346_A3013

Alternate gene names: 237813246

Gene position: 2932173-2931202 (Counterclockwise)

Preceding gene: 237813247

Following gene: 237813242

Centisome position: 71.54

GC content: 65.43

Gene sequence:

>972_bases
ATGAAACAGTATCTCGATCTCGTTCGCACCATTCTCGATACCGGCACCTGGCAGAGCAACCGCACGGGCATTCGCACGAT
CGGCATCCCCGGCGCGATGCTGCGCTTCGATCTGCAGCAGGGCTTTCCCGCAGTGACGACGAAAAAGCTCGCGTTCAAGT
CCGCGATCGGCGAGCTCGTCGGCTTTCTGCGCGCGACGCGCAGCGCGGCCGATTTCCGCGCGCTCGGCTGCAAGGTGTGG
GACGCGAATGCGAATGAGAACGCGCAGTGGCTCGCGAATCCGTATCGCCGCGGCGTCGACGACCTCGGCGACGTGTACGG
CGTCCAATGGCGGCGCTGGCCGGGCTACAAGGTGCTCGACGCGCATGCCGACGCGCAGATCGCCGATGCGACGTCGCGCG
GTTTTCGCGCCGTCGCGCGCTTCGACGAGGACGGCGCGCAGAAGGTGCTGCTGCACAAGGCGATCGACCAGTTGCGAGAC
TGCCTCGACACGATCGTGCGCGACCCGTCGAGCCGGCGCATCCTGTTCCACGGCTGGAATCCCGCGGTGCTCGACGAGAT
CGCGCTGCCCGCGTGCCATCTGCTGTATCAATTCCTGCCGAACGTCGAGCGTCGCGAAATCTCGCTGTGCCTGTACATCC
GCAGCAACGACGTCGGGCTCGGCACGCCGTTCAATCTCGCCGAAGGCGCGGCGCTCCTCACGCTCGTTGGCCGGCTGACG
GGCTATACGCCGCGCTGGTTCACGTACTTCATCGGCGATGCGCACATCTACGAGAATCAGCTCGGCATGCTCAAGCAGCA
GCTCGAACGCGAGCCGTTCGACAGCCCGCGGCTCGAGATCGCCGAGCGCGTGCCCGAGTACGCGAAAACGGGGCAGTACG
AGCCGCAATGGCTTGAACGCATCGAGCCGTCGGATTTCACGCTCGTCGGCTATCGTCACCACGAACCGCTGAGCGCGCCG
ATGGCCGTTTGA

Upstream 100 bases:

>100_bases
GCGCGCGCGCGAAGCGCGACGAGGGGCTGCGCCGGGCCTGCTAGCGCGGGCCTTGTTAAAATGGCGGGCACGGCCGCTTT
TCGCGGCCGGAGGAACCCGC

Downstream 100 bases:

>100_bases
ACGACGCGCGGCCCGTTTCGACCGACCCAAGAAAAAACCCGCGAATTCGCGGGTTTTTTCGTTTGCGTCGATCGACGCGT
GTGACGCGGCTACGCGTGCC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKQYLDLVRTILDTGTWQSNRTGIRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRATRSAADFRALGCKVW
DANANENAQWLANPYRRGVDDLGDVYGVQWRRWPGYKVLDAHADAQIADATSRGFRAVARFDEDGAQKVLLHKAIDQLRD
CLDTIVRDPSSRRILFHGWNPAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLT
GYTPRWFTYFIGDAHIYENQLGMLKQQLEREPFDSPRLEIAERVPEYAKTGQYEPQWLERIEPSDFTLVGYRHHEPLSAP
MAV

Sequences:

>Translated_323_residues
MKQYLDLVRTILDTGTWQSNRTGIRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRATRSAADFRALGCKVW
DANANENAQWLANPYRRGVDDLGDVYGVQWRRWPGYKVLDAHADAQIADATSRGFRAVARFDEDGAQKVLLHKAIDQLRD
CLDTIVRDPSSRRILFHGWNPAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLT
GYTPRWFTYFIGDAHIYENQLGMLKQQLEREPFDSPRLEIAERVPEYAKTGQYEPQWLERIEPSDFTLVGYRHHEPLSAP
MAV
>Mature_323_residues
MKQYLDLVRTILDTGTWQSNRTGIRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRATRSAADFRALGCKVW
DANANENAQWLANPYRRGVDDLGDVYGVQWRRWPGYKVLDAHADAQIADATSRGFRAVARFDEDGAQKVLLHKAIDQLRD
CLDTIVRDPSSRRILFHGWNPAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLT
GYTPRWFTYFIGDAHIYENQLGMLKQQLEREPFDSPRLEIAERVPEYAKTGQYEPQWLERIEPSDFTLVGYRHHEPLSAP
MAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=321, Percent_Identity=35.5140186915888, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI1789191, Length=323, Percent_Identity=35.2941176470588, Blast_Score=174, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=33.5689045936396, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324648, Length=274, Percent_Identity=30.6569343065693, Blast_Score=129, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17137556, Length=321, Percent_Identity=36.1370716510903, Blast_Score=192, Evalue=2e-49,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36622; Mature: 36622

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLVRTILDTGTWQSNRTGIRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELV
CHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GFLRATRSAADFRALGCKVWDANANENAQWLANPYRRGVDDLGDVYGVQWRRWPGYKVLD
HHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHCHHHCCHHHHHHHHCCCEEECCCCEEEE
AHADAQIADATSRGFRAVARFDEDGAQKVLLHKAIDQLRDCLDTIVRDPSSRRILFHGWN
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
PAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLT
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
GYTPRWFTYFIGDAHIYENQLGMLKQQLEREPFDSPRLEIAERVPEYAKTGQYEPQWLER
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHCCCCCHHHHHH
IEPSDFTLVGYRHHEPLSAPMAV
CCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLVRTILDTGTWQSNRTGIRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELV
CHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GFLRATRSAADFRALGCKVWDANANENAQWLANPYRRGVDDLGDVYGVQWRRWPGYKVLD
HHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHCHHHCCHHHHHHHHCCCEEECCCCEEEE
AHADAQIADATSRGFRAVARFDEDGAQKVLLHKAIDQLRDCLDTIVRDPSSRRILFHGWN
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
PAVLDEIALPACHLLYQFLPNVERREISLCLYIRSNDVGLGTPFNLAEGAALLTLVGRLT
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
GYTPRWFTYFIGDAHIYENQLGMLKQQLEREPFDSPRLEIAERVPEYAKTGQYEPQWLER
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHCCCCCHHHHHH
IEPSDFTLVGYRHHEPLSAPMAV
CCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]