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The map label for this gene is puuD [H]

Identifier: 237813074

GI number: 237813074

Start: 2772553

End: 2773584

Strand: Reverse

Name: puuD [H]

Synonym: GBP346_A2836

Alternate gene names: 237813074

Gene position: 2773584-2772553 (Counterclockwise)

Preceding gene: 237813075

Following gene: 237813073

Centisome position: 67.67

GC content: 74.03

Gene sequence:

>1032_bases
GTGACGCGCGCGCGGCGGCGGTGCGCGCATCGCATCGCGCGAAGCTCGGCCCGTGCGTCGCCGCACGCCGTCGCGCGCGG
CCGTCGAATCGGCGCCATGGCGCCGCTGCGCGTCGAATGCGCGCTTGCGCGCAGGCACGCCGCACGTGCGTGCGAGCCGA
TTCCTTCTTTCGCGACGAACGCCGCGCCTTGCGCGCGCCGCGTGCGCGTTCGTCGCGCCTACCGAACGGAGGGACGCGTG
ATGAATGACCCCCTCGGCAACGGACCGCGGCCCGTCGTCGGCATCTGCGCGGATCGCAAGACGGTCGGCTTGCATGTCGC
GCACGTCGCGGGCGAGAAGTACGTGAACGCCGTCGTCGACGGCGCGCACGCGCTCGCGATCGTGCTGCCCGCGCTCGGCG
AGCGGCAGCCCGCGGACGAGTTGCTCGCGCTCGTCGACGGCCTGCTGCTGACGGGCAGCTATTCGAACGTCGAGCCCGCG
CGCTACGGCGGCCCGACGAGCGCGCCGGGCACGCTGCACGACGCGGCGCGCGACGCGACCGCGCTGCCGCTCGTGCGCGC
GGCGATCGACGCCGGCGTGCCCGTGCTCGCGATCTGCCGCGGAATGCAGGAGCTGAACGTCGCGTACGGCGGCACGCTGC
ATCAGCAGGTGCACGCATTGAGCGGACATGCCGACCATCGCGAGGACCTGTCGGTGCCGGTCGACGTGCAGTACGGGCCC
GCGCATCCGGTGCGGCTCGTGCCCGGCGGCCTGCTGCGCCGGCTCGCGGGCGCGGAGACCGTCGAGGTCAATTCTCTGCA
TGCGCAGGGTATCGAACGGCTCGGCGATGGGTTGACCGTCGAGGCCCACGCGCCAGACGGGCTCGTCGAGGCGATCGGCG
TGCGCGGCGCGCGCGCGTTCGCGCTCGGCGTGCAATGGCATCCGGAATGGCGGTTCGACGGCAATGCGCTGTCGCGACAA
ATCCTTGCGGCGTTCGGCGCGGCGTGCCGCGCGCGAAGACGCGCGACGGCCGGGCGCGCGCCGCGCGCGTGA

Upstream 100 bases:

>100_bases
ACATGGCGGTGCACGACGACGGCATCGCGTCGCTGTTGCCCTAGCGCCTCCTTTCTTCTTCGCTCGCCGGCTGGCCGTTC
GCGTGCGCCGTCGCGCTGTC

Downstream 100 bases:

>100_bases
CGGCCGTCGACGCTTATCGAAGAGAGGCTCACATGCAAGACATCGATGATTTTTTGAGGCAGCACCGGATCACCGAAGTG
GAAGCGATCATTCCCGACAT

Product: glutamine amidotransferase, class I

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTRARRRCAHRIARSSARASPHAVARGRRIGAMAPLRVECALARRHAARACEPIPSFATNAAPCARRVRVRRAYRTEGRV
MNDPLGNGPRPVVGICADRKTVGLHVAHVAGEKYVNAVVDGAHALAIVLPALGERQPADELLALVDGLLLTGSYSNVEPA
RYGGPTSAPGTLHDAARDATALPLVRAAIDAGVPVLAICRGMQELNVAYGGTLHQQVHALSGHADHREDLSVPVDVQYGP
AHPVRLVPGGLLRRLAGAETVEVNSLHAQGIERLGDGLTVEAHAPDGLVEAIGVRGARAFALGVQWHPEWRFDGNALSRQ
ILAAFGAACRARRRATAGRAPRA

Sequences:

>Translated_343_residues
MTRARRRCAHRIARSSARASPHAVARGRRIGAMAPLRVECALARRHAARACEPIPSFATNAAPCARRVRVRRAYRTEGRV
MNDPLGNGPRPVVGICADRKTVGLHVAHVAGEKYVNAVVDGAHALAIVLPALGERQPADELLALVDGLLLTGSYSNVEPA
RYGGPTSAPGTLHDAARDATALPLVRAAIDAGVPVLAICRGMQELNVAYGGTLHQQVHALSGHADHREDLSVPVDVQYGP
AHPVRLVPGGLLRRLAGAETVEVNSLHAQGIERLGDGLTVEAHAPDGLVEAIGVRGARAFALGVQWHPEWRFDGNALSRQ
ILAAFGAACRARRRATAGRAPRA
>Mature_342_residues
TRARRRCAHRIARSSARASPHAVARGRRIGAMAPLRVECALARRHAARACEPIPSFATNAAPCARRVRVRRAYRTEGRVM
NDPLGNGPRPVVGICADRKTVGLHVAHVAGEKYVNAVVDGAHALAIVLPALGERQPADELLALVDGLLLTGSYSNVEPAR
YGGPTSAPGTLHDAARDATALPLVRAAIDAGVPVLAICRGMQELNVAYGGTLHQQVHALSGHADHREDLSVPVDVQYGPA
HPVRLVPGGLLRRLAGAETVEVNSLHAQGIERLGDGLTVEAHAPDGLVEAIGVRGARAFALGVQWHPEWRFDGNALSRQI
LAAFGAACRARRRATAGRAPRA

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=243, Percent_Identity=43.2098765432099, Blast_Score=178, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 36300; Mature: 36169

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRARRRCAHRIARSSARASPHAVARGRRIGAMAPLRVECALARRHAARACEPIPSFATN
CCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
AAPCARRVRVRRAYRTEGRVMNDPLGNGPRPVVGICADRKTVGLHVAHVAGEKYVNAVVD
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHC
GAHALAIVLPALGERQPADELLALVDGLLLTGSYSNVEPARYGGPTSAPGTLHDAARDAT
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCCCHHCCCCCCCCCCHHHHHCCHH
ALPLVRAAIDAGVPVLAICRGMQELNVAYGGTLHQQVHALSGHADHREDLSVPVDVQYGP
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCCCCHHCCCCCEEECCCC
AHPVRLVPGGLLRRLAGAETVEVNSLHAQGIERLGDGLTVEAHAPDGLVEAIGVRGARAF
CCCCEECCHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCEEE
ALGVQWHPEWRFDGNALSRQILAAFGAACRARRRATAGRAPRA
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TRARRRCAHRIARSSARASPHAVARGRRIGAMAPLRVECALARRHAARACEPIPSFATN
CHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
AAPCARRVRVRRAYRTEGRVMNDPLGNGPRPVVGICADRKTVGLHVAHVAGEKYVNAVVD
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHC
GAHALAIVLPALGERQPADELLALVDGLLLTGSYSNVEPARYGGPTSAPGTLHDAARDAT
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCCCHHCCCCCCCCCCHHHHHCCHH
ALPLVRAAIDAGVPVLAICRGMQELNVAYGGTLHQQVHALSGHADHREDLSVPVDVQYGP
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCCCCHHCCCCCEEECCCC
AHPVRLVPGGLLRRLAGAETVEVNSLHAQGIERLGDGLTVEAHAPDGLVEAIGVRGARAF
CCCCEECCHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCEEE
ALGVQWHPEWRFDGNALSRQILAAFGAACRARRRATAGRAPRA
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]