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The map label for this gene is shiA [H]

Identifier: 237813065

GI number: 237813065

Start: 2761716

End: 2763047

Strand: Reverse

Name: shiA [H]

Synonym: GBP346_A2826

Alternate gene names: 237813065

Gene position: 2763047-2761716 (Counterclockwise)

Preceding gene: 237813066

Following gene: 237813062

Centisome position: 67.41

GC content: 71.55

Gene sequence:

>1332_bases
ATGACATTCCAGCACGACGAGCGCGCCGAGCGCGCGCCGGTCGGCCAGCCCGCGCGCGCCGCGCTCGCGGCGTTCGTCGG
CACGACGATCGAGTGGTACGACTTCTATCTTTACGCCACCGCGTCCGCGCTCATCTTCGGCAAGCTGTTCTTTCCGTCGG
GCGATCCGTTCTTCAGCACGCTCGCGTCGTTCGGCACGTTCGCTGTCGGCTTTTTCGCGCGGCCGTTCGGCGGCCTCGTG
TTCGGCCACCTCGGCGACCGGGTCGGCCGCAAGAAGGCGCTCGTCGCGACGCTCGTCATCATGGCGGTCGGCACGGTCGG
CATCGGCCTGCTGCCCACGTACGCGCAGGCGGGCGCCGTCGCGCCCGTCGCGCCCGTCGCGCTGCGCATCGCGCAAGGCA
TCGCGATCGGCGGCGAGTGGGGCGGCGCGGTGCTGATGGCGAGCGAGCACGCGAGCGCGGACAGGCGCACGTTCGTCGCG
TCGTTCGCGCAGCTCGGCAGCCCCGCCGGGTTGATTCTCGCGCTACTCGCGTTTCGCCTCGTCGCCGGCATCGAGCATGA
CGCGTTCCTCGCGTGGGGCTGGCGGCTGCCGTTTCTCGCGAGCGCGGCGTTGCTCGTGGTGGGGCTCGCGATCCGCGTGG
GCGTCGACGAATCGCCGGAGTTCGCGCGCGTGAAGGCGCAGCGGCGCACGGCGGCGCTGCCCGTGGCCGACGTGGTGCGC
GACGCCTGGCGCACGCTGTTGCTCTGCCTCGGCGCGAACGTGATCGGCGTCGCGGGCGCGTGGTTCGTCAACACGTTCAT
GCTGAACTACACGACGCAGACGCTCGGGCTCGATCGCGCGCTGATTCTCGACTGCCTGTTCGTCGTCGCGTTCATCCAGC
TCGGCACGCAGCTCGCGTCCGGCTGGTTCGCGCAGCGCATCGGCACCGGGCGCTTCCTGACGGCGGCCGCCGCGCTCGCG
ATCGCGTCGCCGTATCCGATGTTCGCGCTCGTGTCGACGGGGCGCCCCGTGGCGATCGTCGTCGGCATCGCGATCGCGGT
GATGTGCATGTCGAGCTCGTACGCGGTGATGGCGGGCTTCATGTCGACCGCGTTCGACGTGCGTGTGCGCTACTCGGCGA
TCTCGCTGTCGTACCAGCTCTGCGCGGCGCTCGCGGGCGGGCTCACGCCGCTCGCGGGCACGCTGCTCGCGCACCGTTTC
GCCGGCCAGTGGTGGCCGCTCGCCGCGTTCTACAGCGCGCTCGCGGTGGTGTCGCTCGCCTGCATCGCGACGCTCGAGCG
CGGCAAGCGCGTCGGCGTTGGCGAACGGCGCGGAGCGCTCGCGAAGCCTTGA

Upstream 100 bases:

>100_bases
ACGGTTCGCGCCTGCCGGCGCGACCGAATCCGGAGCGATCCGGCCCGACCGGTGAAGGCGCGCGCGAGTCGCGCCGCCGG
CAACAGACATGGAGACAACG

Downstream 100 bases:

>100_bases
CGGCGCCGCGCGGGCCGGCGCGCGCCGCCCTCGGCAAGCGGGTGCGCCGCGTGCCGAGGGCGAGGCGCCCGTAAAAAAAG
CCTCGGGCAGCAATCGCTGC

Product: transporter, major facilitator family

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTFQHDERAERAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGDPFFSTLASFGTFAVGFFARPFGGLV
FGHLGDRVGRKKALVATLVIMAVGTVGIGLLPTYAQAGAVAPVAPVALRIAQGIAIGGEWGGAVLMASEHASADRRTFVA
SFAQLGSPAGLILALLAFRLVAGIEHDAFLAWGWRLPFLASAALLVVGLAIRVGVDESPEFARVKAQRRTAALPVADVVR
DAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQLASGWFAQRIGTGRFLTAAAALA
IASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGFMSTAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRF
AGQWWPLAAFYSALAVVSLACIATLERGKRVGVGERRGALAKP

Sequences:

>Translated_443_residues
MTFQHDERAERAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGDPFFSTLASFGTFAVGFFARPFGGLV
FGHLGDRVGRKKALVATLVIMAVGTVGIGLLPTYAQAGAVAPVAPVALRIAQGIAIGGEWGGAVLMASEHASADRRTFVA
SFAQLGSPAGLILALLAFRLVAGIEHDAFLAWGWRLPFLASAALLVVGLAIRVGVDESPEFARVKAQRRTAALPVADVVR
DAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQLASGWFAQRIGTGRFLTAAAALA
IASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGFMSTAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRF
AGQWWPLAAFYSALAVVSLACIATLERGKRVGVGERRGALAKP
>Mature_442_residues
TFQHDERAERAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGDPFFSTLASFGTFAVGFFARPFGGLVF
GHLGDRVGRKKALVATLVIMAVGTVGIGLLPTYAQAGAVAPVAPVALRIAQGIAIGGEWGGAVLMASEHASADRRTFVAS
FAQLGSPAGLILALLAFRLVAGIEHDAFLAWGWRLPFLASAALLVVGLAIRVGVDESPEFARVKAQRRTAALPVADVVRD
AWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQLASGWFAQRIGTGRFLTAAAALAI
ASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGFMSTAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRFA
GQWWPLAAFYSALAVVSLACIATLERGKRVGVGERRGALAKP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=416, Percent_Identity=34.8557692307692, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1789941, Length=394, Percent_Identity=35.2791878172589, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1790550, Length=423, Percent_Identity=30.2600472813239, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1788942, Length=392, Percent_Identity=31.1224489795918, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87082231, Length=242, Percent_Identity=24.7933884297521, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46606; Mature: 46475

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFQHDERAERAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGDPFFST
CCCCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LASFGTFAVGFFARPFGGLVFGHLGDRVGRKKALVATLVIMAVGTVGIGLLPTYAQAGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
APVAPVALRIAQGIAIGGEWGGAVLMASEHASADRRTFVASFAQLGSPAGLILALLAFRL
CHHHHHHHHHHHCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
VAGIEHDAFLAWGWRLPFLASAALLVVGLAIRVGVDESPEFARVKAQRRTAALPVADVVR
HHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHH
DAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQLAS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GWFAQRIGTGRFLTAAAALAIASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGF
HHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
MSTAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRFAGQWWPLAAFYSALAVVSLA
HHHHHHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CIATLERGKRVGVGERRGALAKP
HHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFQHDERAERAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGDPFFST
CCCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LASFGTFAVGFFARPFGGLVFGHLGDRVGRKKALVATLVIMAVGTVGIGLLPTYAQAGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
APVAPVALRIAQGIAIGGEWGGAVLMASEHASADRRTFVASFAQLGSPAGLILALLAFRL
CHHHHHHHHHHHCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
VAGIEHDAFLAWGWRLPFLASAALLVVGLAIRVGVDESPEFARVKAQRRTAALPVADVVR
HHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHH
DAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQLAS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GWFAQRIGTGRFLTAAAALAIASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGF
HHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
MSTAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRFAGQWWPLAAFYSALAVVSLA
HHHHHHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CIATLERGKRVGVGERRGALAKP
HHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]