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The map label for this gene is glnA [H]

Identifier: 237813053

GI number: 237813053

Start: 2748865

End: 2750280

Strand: Reverse

Name: glnA [H]

Synonym: GBP346_A2814

Alternate gene names: 237813053

Gene position: 2750280-2748865 (Counterclockwise)

Preceding gene: 237813054

Following gene: 237813052

Centisome position: 67.1

GC content: 64.19

Gene sequence:

>1416_bases
ATGAGTAAAACCGTCGCCGACGTCATGCAGCTCGCGAAGGACGAGGACGTCAAGTTTGTCGATTTCCGCTTCACGGACAC
GCGCGGCAAGGAGCAACACGTGTCGGTGCCGGTGTCGGCCTTCGGCGAGGACAAGTTCGAAAGCGGCCACGCGTTCGACG
GCTCGTCGATCGCGGGCTGGAAGGGTATCGAGGCGTCGGACATGCTGCTCATGCCGGATCCGAACGCGGCTTTCATCGAC
CCGTTCTACGAAGAGTCGACCCTCGTGCTGACCTGCGATGTCGTCGAGCCGGCGGACGGCAAGGGCTACGAGCGCGATCC
GCGCTCGCTCGCGAAGCGCGCGGAGGCCTACCTGAAGAGCACGGGCATCGGCGACACCGCGTACTTCGGTCCGGAGCCGG
AATTCTTCATTTTCGACTCGGTGCAGTGGAACACGGACATGTCCGGCTGCTTCGTGAAGATCAACTCGGAAGAAGCGCCG
TGGTCGTCGGCGAAGGAATTCGAAGGCGGCAACACGGGCCACCGTCCGGGCGTGAAGGGCGGCTACTTCCCGGTTGCGCC
GGTCGACCAGTTCCAGGACATGCGCTCGGAAATGTGTCTGCTGCTCGAGCAGATCGGCATTCCGGTCGAAGTGCACCACC
ACGAGGTGGCGGGCCAGGGCCAGAACGAAATCGGCACGAAGTTCTCGACGCTCGTCGAGCGCGCGGACTGGACGCAATGG
GCGAAGTACATCATCCATAACGTCGCGCACTCGTACGGCAAGACGGCGACGTTCATGCCGAAGCCCGTCGTCGGCGACAA
CGGCTCGGGCATGCACGTGCACCAGTCGATCTGGAAGGACGGCCAGAACCTGTTCGCGGGCAACGGCTACGCGGGCCTGT
CGGAGACGGCGCTCTTCTACATCGGCGGCATCATCAAGCACGCGCGCGCGCTGAACGCGATCACGAACCCGACGACGAAC
TCGTACAAGCGTCTCGTGCCGCACTTCGAAGCGCCCGTGAAGCTCGCCTACTCGGCGCGCAACCGCTCGGCGTCGATCCG
CATTCCGCACGTGTCGAACCCGAAGGGCCGCCGCATCGAAACGCGCTTCCCGGACCCGATGGCGAACCCGTACCTGTGCT
TCTCGGCGCTGATGATGGCGGGTCTCGACGGCATCCAGAACAAGATCCATCCGGGCGAGGCCGCGGACAAGAACCTGTAC
GACCTGCCGCCGGAAGAGGATGCGAAGATCCCGACCGTGTGCGCCGGCCTCGACCAGGCGCTCGAAGCGCTCGACAAGGA
CCGCGAGTTCCTGACGCGCGGCGGCGTGTTCACGGACGCGATGATCGACGCGTACCTGGGCCTGAAGGAGCAGGAGCTCG
CGAAGTTCCGCATGACGACGCACCCGATCGAGTTCGAGATGTACTACTCGCTGTAA

Upstream 100 bases:

>100_bases
GTTCGAATCGGTTGTACGCGGCAAAGAACGCTGCGCTCAGCAGCTCGCTTAACGAGGCGGTCCCAGTCCCGCCGGATTTG
TTCAATCAGGAGAAGAGGTT

Downstream 100 bases:

>100_bases
GCGGCGATGGCGCTTCGTCTCGTCGGCGGACGAAGCGCCGCGGCGCCGACCGGCCTTCGCGCGGCGACTGCGCGCGAAGG
CATCGGACCTTGAAAGGGGA

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSKTVADVMQLAKDEDVKFVDFRFTDTRGKEQHVSVPVSAFGEDKFESGHAFDGSSIAGWKGIEASDMLLMPDPNAAFID
PFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGIGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAP
WSSAKEFEGGNTGHRPGVKGGYFPVAPVDQFQDMRSEMCLLLEQIGIPVEVHHHEVAGQGQNEIGTKFSTLVERADWTQW
AKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSETALFYIGGIIKHARALNAITNPTTN
SYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLCFSALMMAGLDGIQNKIHPGEAADKNLY
DLPPEEDAKIPTVCAGLDQALEALDKDREFLTRGGVFTDAMIDAYLGLKEQELAKFRMTTHPIEFEMYYSL

Sequences:

>Translated_471_residues
MSKTVADVMQLAKDEDVKFVDFRFTDTRGKEQHVSVPVSAFGEDKFESGHAFDGSSIAGWKGIEASDMLLMPDPNAAFID
PFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGIGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAP
WSSAKEFEGGNTGHRPGVKGGYFPVAPVDQFQDMRSEMCLLLEQIGIPVEVHHHEVAGQGQNEIGTKFSTLVERADWTQW
AKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSETALFYIGGIIKHARALNAITNPTTN
SYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLCFSALMMAGLDGIQNKIHPGEAADKNLY
DLPPEEDAKIPTVCAGLDQALEALDKDREFLTRGGVFTDAMIDAYLGLKEQELAKFRMTTHPIEFEMYYSL
>Mature_470_residues
SKTVADVMQLAKDEDVKFVDFRFTDTRGKEQHVSVPVSAFGEDKFESGHAFDGSSIAGWKGIEASDMLLMPDPNAAFIDP
FYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGIGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAPW
SSAKEFEGGNTGHRPGVKGGYFPVAPVDQFQDMRSEMCLLLEQIGIPVEVHHHEVAGQGQNEIGTKFSTLVERADWTQWA
KYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSETALFYIGGIIKHARALNAITNPTTNS
YKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLCFSALMMAGLDGIQNKIHPGEAADKNLYD
LPPEEDAKIPTVCAGLDQALEALDKDREFLTRGGVFTDAMIDAYLGLKEQELAKFRMTTHPIEFEMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=65.5172413793103, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=407, Percent_Identity=24.8157248157248, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52189; Mature: 52058

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTVADVMQLAKDEDVKFVDFRFTDTRGKEQHVSVPVSAFGEDKFESGHAFDGSSIAGW
CCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMLLMPDPNAAFIDPFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKS
CCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TGIGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAPWSSAKEFEGGNTGHRPGVKG
CCCCCCCCCCCCCCEEEEECEEECCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCC
GYFPVAPVDQFQDMRSEMCLLLEQIGIPVEVHHHEVAGQGQNEIGTKFSTLVERADWTQW
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHH
AKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSETALFY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCCHHHHHHH
IGGIIKHARALNAITNPTTNSYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIE
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEEE
TRFPDPMANPYLCFSALMMAGLDGIQNKIHPGEAADKNLYDLPPEEDAKIPTVCAGLDQA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LEALDKDREFLTRGGVFTDAMIDAYLGLKEQELAKFRMTTHPIEFEMYYSL
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
SKTVADVMQLAKDEDVKFVDFRFTDTRGKEQHVSVPVSAFGEDKFESGHAFDGSSIAGW
CCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMLLMPDPNAAFIDPFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKS
CCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TGIGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAPWSSAKEFEGGNTGHRPGVKG
CCCCCCCCCCCCCCEEEEECEEECCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCCC
GYFPVAPVDQFQDMRSEMCLLLEQIGIPVEVHHHEVAGQGQNEIGTKFSTLVERADWTQW
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHH
AKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSETALFY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCCHHHHHHH
IGGIIKHARALNAITNPTTNSYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIE
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEEE
TRFPDPMANPYLCFSALMMAGLDGIQNKIHPGEAADKNLYDLPPEEDAKIPTVCAGLDQA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LEALDKDREFLTRGGVFTDAMIDAYLGLKEQELAKFRMTTHPIEFEMYYSL
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]