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The map label for this gene is xth_2 [H]

Identifier: 237813050

GI number: 237813050

Start: 2744918

End: 2745694

Strand: Direct

Name: xth_2 [H]

Synonym: GBP346_A2811

Alternate gene names: 237813050

Gene position: 2744918-2745694 (Clockwise)

Preceding gene: 237813049

Following gene: 237813055

Centisome position: 66.97

GC content: 66.02

Gene sequence:

>777_bases
ATGAGAATCGCCACCTGGAACGTCAACTCCCTGAACGTGCGCAAGCAGCACGTGCTCGACTGGCTCGCGCAGAGCGACGT
CGACGTGCTGTGCCTTCAGGAGCTCAAGATCCCCGACGAGAAGTTTCCGCGCGAAGCGCTCGAAGCGGCCGGCTACCGCA
GCTGGTTCGCGGGCCAGAAAACCTACAACGGCGTCGCGATCCTCGCGCGCGCGAGCCTGCCGTTCGACGAGACGGATATC
GTTCGCAACATCCCCGGCTTCGAGGACGCGCAGCAGCGCCTGATCGCCGCGACGATCGACGGCGTGCGGATCGTGTCCGC
GTACTTCCCGAACGGCCAGGCGCTCGACTCGGACAAGTTCGTCTACAAGATGCAATGGCTCGACGCGCTGCAGGCATGGC
TGAAGGACGAGCTGCAACGCTACCCGAAGCTCGCGCTGCTCGGCGACTACAACATCGCGCCCGAAGATCGCGACGTGCAC
GATCCGGCGAAATGGGAAGGCCAGAATCTGGTGTCGCCGCAGGAGCGCGCGCATTTCGCGCAGCTCGTCGCGCTCGGCTT
CGTCGACGCGTTCCGACGCTTCGAGCAACCCGAGAAGACCTTCACGTGGTGGGATTACCGGATGCTCGCGTTCCGCCGCA
ACGCCGGGCTGCGGATCGATCACATCCTGCTGTCGCCGGCGCTCGCCGAAACCTGCACGAGCTGCACCGTCGACCGCGTG
CCGCGCACGTGGGAGCAGCCGTCCGACCACACGCCCGTCGTCGCGCAGCTCGGCTGA

Upstream 100 bases:

>100_bases
GCGCCGGCGGCCCGCCGCGGCGGCGTCTCGCCCCCCTTTCCCGTCGGCCGCGGCGGCTCGCGGTAAGATGTCGGCCTCAC
GCATCCCGCCGATCCCGCCC

Downstream 100 bases:

>100_bases
GCGTGCGCCGCTGCGCGTGATCGCGCCCGCGCGCCGGCCCGCGTGCCGCGGCGCGCGAGGAGGCGCGCGGTTGCGCGTGC
GAAGAGCGGTTCGCCGAGCG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRIATWNVNSLNVRKQHVLDWLAQSDVDVLCLQELKIPDEKFPREALEAAGYRSWFAGQKTYNGVAILARASLPFDETDI
VRNIPGFEDAQQRLIAATIDGVRIVSAYFPNGQALDSDKFVYKMQWLDALQAWLKDELQRYPKLALLGDYNIAPEDRDVH
DPAKWEGQNLVSPQERAHFAQLVALGFVDAFRRFEQPEKTFTWWDYRMLAFRRNAGLRIDHILLSPALAETCTSCTVDRV
PRTWEQPSDHTPVVAQLG

Sequences:

>Translated_258_residues
MRIATWNVNSLNVRKQHVLDWLAQSDVDVLCLQELKIPDEKFPREALEAAGYRSWFAGQKTYNGVAILARASLPFDETDI
VRNIPGFEDAQQRLIAATIDGVRIVSAYFPNGQALDSDKFVYKMQWLDALQAWLKDELQRYPKLALLGDYNIAPEDRDVH
DPAKWEGQNLVSPQERAHFAQLVALGFVDAFRRFEQPEKTFTWWDYRMLAFRRNAGLRIDHILLSPALAETCTSCTVDRV
PRTWEQPSDHTPVVAQLG
>Mature_258_residues
MRIATWNVNSLNVRKQHVLDWLAQSDVDVLCLQELKIPDEKFPREALEAAGYRSWFAGQKTYNGVAILARASLPFDETDI
VRNIPGFEDAQQRLIAATIDGVRIVSAYFPNGQALDSDKFVYKMQWLDALQAWLKDELQRYPKLALLGDYNIAPEDRDVH
DPAKWEGQNLVSPQERAHFAQLVALGFVDAFRRFEQPEKTFTWWDYRMLAFRRNAGLRIDHILLSPALAETCTSCTVDRV
PRTWEQPSDHTPVVAQLG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=265, Percent_Identity=32.4528301886792, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI18375503, Length=265, Percent_Identity=32.4528301886792, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI18375501, Length=265, Percent_Identity=32.4528301886792, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=36.0902255639098, Blast_Score=157, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71989536, Length=268, Percent_Identity=25.7462686567164, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221330655, Length=258, Percent_Identity=29.0697674418605, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17136678, Length=258, Percent_Identity=29.0697674418605, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29587; Mature: 29587

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNVNSLNVRKQHVLDWLAQSDVDVLCLQELKIPDEKFPREALEAAGYRSWFAGQK
CEEEEECCCCCCHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCHHHHHHCCCHHHHCCCC
TYNGVAILARASLPFDETDIVRNIPGFEDAQQRLIAATIDGVRIVSAYFPNGQALDSDKF
CCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCH
VYKMQWLDALQAWLKDELQRYPKLALLGDYNIAPEDRDVHDPAKWEGQNLVSPQERAHFA
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QLVALGFVDAFRRFEQPEKTFTWWDYRMLAFRRNAGLRIDHILLSPALAETCTSCTVDRV
HHHHHHHHHHHHHHCCCCCCEEHHHHEEEEECCCCCCEEHHHHHCHHHHHHHHHCCHHHC
PRTWEQPSDHTPVVAQLG
CCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MRIATWNVNSLNVRKQHVLDWLAQSDVDVLCLQELKIPDEKFPREALEAAGYRSWFAGQK
CEEEEECCCCCCHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCHHHHHHCCCHHHHCCCC
TYNGVAILARASLPFDETDIVRNIPGFEDAQQRLIAATIDGVRIVSAYFPNGQALDSDKF
CCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCH
VYKMQWLDALQAWLKDELQRYPKLALLGDYNIAPEDRDVHDPAKWEGQNLVSPQERAHFA
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QLVALGFVDAFRRFEQPEKTFTWWDYRMLAFRRNAGLRIDHILLSPALAETCTSCTVDRV
HHHHHHHHHHHHHHCCCCCCEEHHHHEEEEECCCCCCEEHHHHHCHHHHHHHHHCCHHHC
PRTWEQPSDHTPVVAQLG
CCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]