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The map label for this gene is narI_1 [H]

Identifier: 237813047

GI number: 237813047

Start: 2741285

End: 2741971

Strand: Direct

Name: narI_1 [H]

Synonym: GBP346_A2808

Alternate gene names: 237813047

Gene position: 2741285-2741971 (Clockwise)

Preceding gene: 237813046

Following gene: 237813048

Centisome position: 66.88

GC content: 67.54

Gene sequence:

>687_bases
ATGGATTATCTGAATACGTTCGTCTACGGCATCTATCCGTACATCGCCGCCGCCATCTTCCTGTTCGGCAGCCTCGCGCG
CTTCGAGCGCGAGCAGTACACATGGAAGTCCGACAGCTCGCAGCTGCTGCACCGCGGCGCGCTGCGCACGGGCAACATCC
TTTTTCACGTCGGCGTGCTCGGCCTGTTCTTCGGGCATCTGGTCGGTCTGCTCACGCCGGTGGCCGTCTGGGACGCGCTC
GGCGTCACGCACCAGTTCAAGCAGCTCGTCGCGATGTCCGCGGGCGGCGTGATGGGCTCGCTCGCGCTCGCGGGCCTCGC
GATCCTGCTGCACCGGCGCCTGACGAACGCGCGCCTCGCGGCCGTCACGCGCCGCGGCGACAAGGTGCTGCTCGCGTGGC
TGCTCGTCACGTTGGTGCTCGGGCTGTCGACGATCTTCGAATCGGCGAGCCACACCGACGGCCACCTGATGGTGCAACTG
ATGACGTGGGCGCAGCACATCGTCACGTTCCGCGGCGACGCGGCGAGCTACGTCGCCGATGCGCCGTGGCTCTTCAAGGC
GCACCTGTTCATGGGGCTCTCGCTCTTCGCGATCTTCCCGTTCACGCGGCTCGTTCACGTATGGAGCGGCTTCGCGTCGG
TCACCTACGTCGGCCGCGCGTGGCAGCTCGTGCGCGCGCGCCGCTAG

Upstream 100 bases:

>100_bases
GCTCGCGCAGCCGATCCGCTTTCACCCGCCGCGCCGCGCGGCGACGCCCGCTCCCCGCGCGTGACGCGGGGGCGTCGCGA
ACCCGTTTCGAGGTCACGTC

Downstream 100 bases:

>100_bases
GCGCCGCTCGCGCGGGGTCGCAGTCCAGGCATCGCGGCGCACCGGGCGGGTGCGCCGCGATGCCTCACGTGCTCGAATGT
CGCAGCGGGGGCGCAACATT

Product: respiratory nitrate reductase, gamma subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MDYLNTFVYGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQLLHRGALRTGNILFHVGVLGLFFGHLVGLLTPVAVWDAL
GVTHQFKQLVAMSAGGVMGSLALAGLAILLHRRLTNARLAAVTRRGDKVLLAWLLVTLVLGLSTIFESASHTDGHLMVQL
MTWAQHIVTFRGDAASYVADAPWLFKAHLFMGLSLFAIFPFTRLVHVWSGFASVTYVGRAWQLVRARR

Sequences:

>Translated_228_residues
MDYLNTFVYGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQLLHRGALRTGNILFHVGVLGLFFGHLVGLLTPVAVWDAL
GVTHQFKQLVAMSAGGVMGSLALAGLAILLHRRLTNARLAAVTRRGDKVLLAWLLVTLVLGLSTIFESASHTDGHLMVQL
MTWAQHIVTFRGDAASYVADAPWLFKAHLFMGLSLFAIFPFTRLVHVWSGFASVTYVGRAWQLVRARR
>Mature_228_residues
MDYLNTFVYGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQLLHRGALRTGNILFHVGVLGLFFGHLVGLLTPVAVWDAL
GVTHQFKQLVAMSAGGVMGSLALAGLAILLHRRLTNARLAAVTRRGDKVLLAWLLVTLVLGLSTIFESASHTDGHLMVQL
MTWAQHIVTFRGDAASYVADAPWLFKAHLFMGLSLFAIFPFTRLVHVWSGFASVTYVGRAWQLVRARR

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transf

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=228, Percent_Identity=47.3684210526316, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI1787480, Length=227, Percent_Identity=45.8149779735683, Blast_Score=199, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25322; Mature: 25322

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLNTFVYGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQLLHRGALRTGNILFHVGVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
GLFFGHLVGLLTPVAVWDALGVTHQFKQLVAMSAGGVMGSLALAGLAILLHRRLTNARLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AVTRRGDKVLLAWLLVTLVLGLSTIFESASHTDGHLMVQLMTWAQHIVTFRGDAASYVAD
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHC
APWLFKAHLFMGLSLFAIFPFTRLVHVWSGFASVTYVGRAWQLVRARR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYLNTFVYGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQLLHRGALRTGNILFHVGVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
GLFFGHLVGLLTPVAVWDALGVTHQFKQLVAMSAGGVMGSLALAGLAILLHRRLTNARLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AVTRRGDKVLLAWLLVTLVLGLSTIFESASHTDGHLMVQLMTWAQHIVTFRGDAASYVAD
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHC
APWLFKAHLFMGLSLFAIFPFTRLVHVWSGFASVTYVGRAWQLVRARR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]