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The map label for this gene is narH_1 [H]

Identifier: 237813045

GI number: 237813045

Start: 2738994

End: 2740547

Strand: Direct

Name: narH_1 [H]

Synonym: GBP346_A2806

Alternate gene names: 237813045

Gene position: 2738994-2740547 (Clockwise)

Preceding gene: 237813044

Following gene: 237813046

Centisome position: 66.83

GC content: 65.7

Gene sequence:

>1554_bases
ATGAAGATCCGCGCACAAGTGGCGATGGTGCTCAACCTCGACAAATGCATCGGTTGCCATACCTGCTCCGTCACCTGCAA
GAACGTGTGGACCTCGCGCGACGGCGTCGAGTACGCGTGGTTCAACAACGTCGAGACGAAGCCGGGCATCGGCTATCCGC
GCGAGTGGGAGAACCAGGACAAGTGGCAAGGCGGCTGGAAGCGCAACGACGCCGGCAAGCTCGAGCCGCGCCAGGGCGGC
AAGCTGAAGATCCTCGCGAACCTCTTCGCGAATCCGAACCTGCCCGCGATCGACGACTACTACGAGCCGTTCACGTACGA
CTACGCGCATCTGCAGAACGCGCCGCTGTCGCAGACGCCGCCGACCGCGCGCCCCGTGTCCGCGATCACCGGCCAGAAGA
TGGAGAAGATCGAATGGGGCCCGAACTGGGAAGACGACCTCGGCGGCGAGTTCAAGTCGCGCAGCCGCGACAAGCTGTTC
GAGGCCGTGCAAAAGGAGATGTACTCGACGTTCGAGAACACCTTCATGATGTATCTGCCGCGCCTCTGCGAGCACTGCCT
GAACCCGACCTGCGTCGCGTCGTGCCCGTCGGGCTCGGTCTACAAGCGCGAGGACGACGGCATCGTGCTCGTCGACCAGG
ACAAGTGCCGCGGCTGGCGGATGTGCGTCTCCGGCTGCCCGTACAAGAAGATCTACTTCAACTGGCAGACCGGCAAGGCC
GAGAAGTGCGTGTTCTGCTTCCCGCGCATCGAGGCCGGCCAGCCGACCGTATGCTCGGAAACCTGCGTCGGGCGCATCCG
CTATCTCGGCGTGATGCTCTATGACGCGGACCGCATCGAGCAGGCCGCCTCCGTCGCCGATCCGCAGAAGCTCTACGAAG
AGCAGCTCCGCGTGTTCGTCGATCCGCACGATCCGGTAATCGAGCGCCAGGCGCTCGCCGACGGCGTCCCGCAAAGCTGG
CTCGACGCGGCCAAGCGCTCGCCCGTCTACCGGATGGCGTGCGAATGGAAGGTCGCGTTCCCGCTGCACCCGGAATACCG
GACGCTGCCGATGGTCTGGTACGTGCCGCCGCTCTCGCCGATCCAGGGCGCCGCGCAGTCCGGCCACATGGGGATGAACG
GCGTGATTCCGGACGTGCGCAGCCTGCGGATTCCGCTGCGCTATCTCGCGAACCTGCTGACGGCGGGCGACGAGGCGCCC
GTCGCCGCCGCGCTCGACCGCATGCTCGCGATGCGCGCGTACAAGCGCGCGCAAACCGTGCACGGCGCGCACGACGCGCA
GGTGCTCGAACAAGTGGGGCTCACCGCCGAGCAGGTGGAGGACATGTATCAGCTGATGGCGATCGCGAACTACGAGGACC
GCTTCGTGATTCCGTCGTCGCACAAGGAAATGGCGGAAGACAGCTTCGACGAGAAAGGCAGCTGCGGCTTCTCGTTCGGC
AACGGCTGCTCGGGCGGCACATCGGACGGCACGCTGTTCGGCCGCAAGAAGGAAACGGCGGTGTCGTACGCGGCGCTGCC
GACCGTCACGCGCAAGAAAGCGAGCGCCGCATGA

Upstream 100 bases:

>100_bases
ACTACGGCACCGTCGGCAGCAACCGCGACGAATACGTGATCGTGCGCAAGATGAACCGGGTGGACTGGCTCGAAGAGCCG
CTTAACGAAGGAGCAGAACA

Downstream 100 bases:

>100_bases
GCGCATCGCACGAGGAAAACGTCATGTCGATCTATCCGATTCTGTCCGCCCTGCTCACCTATCCCGAGCAGCCGCTGCTC
GACGCGCTGCCCGAGATCGA

Product: nitrate reductase, beta subunit

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPREWENQDKWQGGWKRNDAGKLEPRQGG
KLKILANLFANPNLPAIDDYYEPFTYDYAHLQNAPLSQTPPTARPVSAITGQKMEKIEWGPNWEDDLGGEFKSRSRDKLF
EAVQKEMYSTFENTFMMYLPRLCEHCLNPTCVASCPSGSVYKREDDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWQTGKA
EKCVFCFPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADPQKLYEEQLRVFVDPHDPVIERQALADGVPQSW
LDAAKRSPVYRMACEWKVAFPLHPEYRTLPMVWYVPPLSPIQGAAQSGHMGMNGVIPDVRSLRIPLRYLANLLTAGDEAP
VAAALDRMLAMRAYKRAQTVHGAHDAQVLEQVGLTAEQVEDMYQLMAIANYEDRFVIPSSHKEMAEDSFDEKGSCGFSFG
NGCSGGTSDGTLFGRKKETAVSYAALPTVTRKKASAA

Sequences:

>Translated_517_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPREWENQDKWQGGWKRNDAGKLEPRQGG
KLKILANLFANPNLPAIDDYYEPFTYDYAHLQNAPLSQTPPTARPVSAITGQKMEKIEWGPNWEDDLGGEFKSRSRDKLF
EAVQKEMYSTFENTFMMYLPRLCEHCLNPTCVASCPSGSVYKREDDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWQTGKA
EKCVFCFPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADPQKLYEEQLRVFVDPHDPVIERQALADGVPQSW
LDAAKRSPVYRMACEWKVAFPLHPEYRTLPMVWYVPPLSPIQGAAQSGHMGMNGVIPDVRSLRIPLRYLANLLTAGDEAP
VAAALDRMLAMRAYKRAQTVHGAHDAQVLEQVGLTAEQVEDMYQLMAIANYEDRFVIPSSHKEMAEDSFDEKGSCGFSFG
NGCSGGTSDGTLFGRKKETAVSYAALPTVTRKKASAA
>Mature_517_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPREWENQDKWQGGWKRNDAGKLEPRQGG
KLKILANLFANPNLPAIDDYYEPFTYDYAHLQNAPLSQTPPTARPVSAITGQKMEKIEWGPNWEDDLGGEFKSRSRDKLF
EAVQKEMYSTFENTFMMYLPRLCEHCLNPTCVASCPSGSVYKREDDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWQTGKA
EKCVFCFPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADPQKLYEEQLRVFVDPHDPVIERQALADGVPQSW
LDAAKRSPVYRMACEWKVAFPLHPEYRTLPMVWYVPPLSPIQGAAQSGHMGMNGVIPDVRSLRIPLRYLANLLTAGDEAP
VAAALDRMLAMRAYKRAQTVHGAHDAQVLEQVGLTAEQVEDMYQLMAIANYEDRFVIPSSHKEMAEDSFDEKGSCGFSFG
NGCSGGTSDGTLFGRKKETAVSYAALPTVTRKKASAA

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=68.1818181818182, Blast_Score=767, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=517, Percent_Identity=66.1508704061896, Blast_Score=760, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=162, Percent_Identity=32.0987654320988, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=34.0909090909091, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367345, Length=101, Percent_Identity=31.6831683168317, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787749, Length=102, Percent_Identity=32.3529411764706, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=36.046511627907, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=35.0515463917526, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=32.183908045977, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57995; Mature: 57995

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPREWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRNDAGKLEPRQGGKLKILANLFANPNLPAIDDYYEPFTYDYAHLQNAPLSQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPVSAITGQKMEKIEWGPNWEDDLGGEFKSRSRDKLFEAVQKEMYSTFENTFMMYLP
CCCCCHHHHHCCHHHHCCCCCCCCHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPTCVASCPSGSVYKREDDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWQTGKA
HHHHHHCCCEEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCVFCFPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADPQKLYEEQLRVFV
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHCCCHHHHHHHHHEEEE
DPHDPVIERQALADGVPQSWLDAAKRSPVYRMACEWKVAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCH
IQGAAQSGHMGMNGVIPDVRSLRIPLRYLANLLTAGDEAPVAAALDRMLAMRAYKRAQTV
HHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
HGAHDAQVLEQVGLTAEQVEDMYQLMAIANYEDRFVIPSSHKEMAEDSFDEKGSCGFSFG
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCCCCC
NGCSGGTSDGTLFGRKKETAVSYAALPTVTRKKASAA
CCCCCCCCCCEEECCCHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPREWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRNDAGKLEPRQGGKLKILANLFANPNLPAIDDYYEPFTYDYAHLQNAPLSQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPVSAITGQKMEKIEWGPNWEDDLGGEFKSRSRDKLFEAVQKEMYSTFENTFMMYLP
CCCCCHHHHHCCHHHHCCCCCCCCHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPTCVASCPSGSVYKREDDGIVLVDQDKCRGWRMCVSGCPYKKIYFNWQTGKA
HHHHHHCCCEEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCVFCFPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADPQKLYEEQLRVFV
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHCCCHHHHHHHHHEEEE
DPHDPVIERQALADGVPQSWLDAAKRSPVYRMACEWKVAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCH
IQGAAQSGHMGMNGVIPDVRSLRIPLRYLANLLTAGDEAPVAAALDRMLAMRAYKRAQTV
HHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
HGAHDAQVLEQVGLTAEQVEDMYQLMAIANYEDRFVIPSSHKEMAEDSFDEKGSCGFSFG
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCCCCC
NGCSGGTSDGTLFGRKKETAVSYAALPTVTRKKASAA
CCCCCCCCCCEEECCCHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]