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The map label for this gene is trmJ [H]

Identifier: 237812913

GI number: 237812913

Start: 2615868

End: 2616884

Strand: Reverse

Name: trmJ [H]

Synonym: GBP346_A2673

Alternate gene names: 237812913

Gene position: 2616884-2615868 (Counterclockwise)

Preceding gene: 237812915

Following gene: 237812912

Centisome position: 63.85

GC content: 70.21

Gene sequence:

>1017_bases
TTGCGAAACGGTTGCGCGGGCCGCTCGATCGGCCGTTCGGACGAGTGGGTTTGTCGGCGAGTTGGCGATACCATACCGGC
ATCCCGATCAGTCGAAGTGAACGTTTTGGAATCTCAGCAGAAACCGTCCGCGCCGCCTTCCGGCGCGGCCACATCCCGTC
CGGCGCGGACCGCGCGCGGCGGATTCACGTCGACGCGCTTCGTGCTCGTCGAGCCGAGCCACCCCGGCAACGTCGGCGCG
GCGGCGCGCGCGCTGAAGACGATGGGGTTTTCCCGCCTCGTGCTCGTCGCGCCGCGCGTGCCGCGCGTGCAGAGCGATCC
GGAGGCGCTCGCGATGGCGAGCGGCGCCGACGATGTGCTCGCGTCCGCGCACGTCGTGCCGACGCTCGCCGACGCGCTGA
ACGGCGTGCAGTGGTCGCTCGCGCTCACCGCGCGTTCGCGCGAGTACGGCCCGCCGCGGCTCGCGCCCCGCGCGGCCGCC
GCGAGCGCGCTTCAGCAGGTGCGCACGGGCGATATCGCGCTCGTGTTCGGCAACGAACGCACCGGCCTGTCGAACGAGCA
CGTCGAGCGCTGCAGCGCGATCGCGCACATTCCGGCGAACCCCGCATACAGTTCGCTCAACCTCGCGCAGGCGGTGCAGG
TGCTCGCGTACGAGCTGCGCGTCGCGCTGCTCGAAGACGACGAGTCGGCGGTGCCGCCGGACGCCGCGCTCGGCACGCTC
GCGCAAAGCGACGAGATCGAGCGGATGTTCGTCCATCTGGAAAATGCGCTGATCGCGCTCGATTTCCTCGATCCGCGCAA
TCCGAAGAAGCTGATGCCGCGGCTGCGGCGGCTCTTCGCGCGAACGGGGCTCGAACGCGAAGAAGTGAACATCGTTCGAG
GCATCGCGAAGCACATCCTGCTGAAGGCGGGCGGGGCGAACGCGCGCGCGGGCGCTTCGGGCGAGGGCAACGGCAACGGC
GAAAGCGCCACCGGCGACGCGGACTATTCGGGTCGCGACGTCAGCCGAGACGACTGA

Upstream 100 bases:

>100_bases
TTTCGCCGACGGAAGACGAATTGGCAAAGAGCGGGGTGCGGGCCGAGCAACGCAAGCGCCGCATGCGAAAGCGTAATTTT
ACCCGACTCGCGGCATTTTT

Downstream 100 bases:

>100_bases
CGCCGCGCGGTCTCGCGGTCGTGCCATACCCGCGATCGCGGCAGGGGGAACGCGGCGCCTGCTGCGCGTGCGCGGCGGCG
GCTCTACAATCGTCCGCATG

Product: RNA methyltransferase, TrmH family, group 1

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MRNGCAGRSIGRSDEWVCRRVGDTIPASRSVEVNVLESQQKPSAPPSGAATSRPARTARGGFTSTRFVLVEPSHPGNVGA
AARALKTMGFSRLVLVAPRVPRVQSDPEALAMASGADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAA
ASALQQVRTGDIALVFGNERTGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVLAYELRVALLEDDESAVPPDAALGTL
AQSDEIERMFVHLENALIALDFLDPRNPKKLMPRLRRLFARTGLEREEVNIVRGIAKHILLKAGGANARAGASGEGNGNG
ESATGDADYSGRDVSRDD

Sequences:

>Translated_338_residues
MRNGCAGRSIGRSDEWVCRRVGDTIPASRSVEVNVLESQQKPSAPPSGAATSRPARTARGGFTSTRFVLVEPSHPGNVGA
AARALKTMGFSRLVLVAPRVPRVQSDPEALAMASGADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAA
ASALQQVRTGDIALVFGNERTGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVLAYELRVALLEDDESAVPPDAALGTL
AQSDEIERMFVHLENALIALDFLDPRNPKKLMPRLRRLFARTGLEREEVNIVRGIAKHILLKAGGANARAGASGEGNGNG
ESATGDADYSGRDVSRDD
>Mature_338_residues
MRNGCAGRSIGRSDEWVCRRVGDTIPASRSVEVNVLESQQKPSAPPSGAATSRPARTARGGFTSTRFVLVEPSHPGNVGA
AARALKTMGFSRLVLVAPRVPRVQSDPEALAMASGADDVLASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAA
ASALQQVRTGDIALVFGNERTGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVLAYELRVALLEDDESAVPPDAALGTL
AQSDEIERMFVHLENALIALDFLDPRNPKKLMPRLRRLFARTGLEREEVNIVRGIAKHILLKAGGANARAGASGEGNGNG
ESATGDADYSGRDVSRDD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=247, Percent_Identity=43.3198380566802, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1790865, Length=158, Percent_Identity=39.2405063291139, Blast_Score=95, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 35731; Mature: 35731

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNGCAGRSIGRSDEWVCRRVGDTIPASRSVEVNVLESQQKPSAPPSGAATSRPARTARG
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHCC
GFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPRVQSDPEALAMASGADDVL
CCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHEECCCCHHHH
ASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAAASALQQVRTGDIALVFGNER
HHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
TGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVLAYELRVALLEDDESAVPPDAALGTL
CCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHH
AQSDEIERMFVHLENALIALDFLDPRNPKKLMPRLRRLFARTGLEREEVNIVRGIAKHIL
HHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LKAGGANARAGASGEGNGNGESATGDADYSGRDVSRDD
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNGCAGRSIGRSDEWVCRRVGDTIPASRSVEVNVLESQQKPSAPPSGAATSRPARTARG
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHCC
GFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPRVQSDPEALAMASGADDVL
CCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHEECCCCHHHH
ASAHVVPTLADALNGVQWSLALTARSREYGPPRLAPRAAAASALQQVRTGDIALVFGNER
HHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
TGLSNEHVERCSAIAHIPANPAYSSLNLAQAVQVLAYELRVALLEDDESAVPPDAALGTL
CCCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHH
AQSDEIERMFVHLENALIALDFLDPRNPKKLMPRLRRLFARTGLEREEVNIVRGIAKHIL
HHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LKAGGANARAGASGEGNGNGESATGDADYSGRDVSRDD
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA