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The map label for this gene is alkA [H]

Identifier: 237812905

GI number: 237812905

Start: 2609177

End: 2610115

Strand: Reverse

Name: alkA [H]

Synonym: GBP346_A2665

Alternate gene names: 237812905

Gene position: 2610115-2609177 (Counterclockwise)

Preceding gene: 237812906

Following gene: 237812904

Centisome position: 63.68

GC content: 69.12

Gene sequence:

>939_bases
ATGGCAACGGCCAGAAAAGCGCCGGCTAAGCGTGCGGCGTCGCAGCCGCCGATGGCGGCGAAGCGGGCCGGCGCGCGTGC
GCCGGCGGTGCACAAGGCGGGCGCGAAGCGCGCGGCGCCGAACGGCGCGGCCAATGGCGCCGCGCATCAGCCGGGCTTGC
GCGCGGCGGCCGGCAAGCCGGCGCGCGCGAAGGTCGACGGCGAGCGCGCCGTCGACGCGCTGCCGCGCGAGGCCGCCGCC
GTCGCGGCGGAGCCCGTCGCGCGCAAGGCGCGCGTGTCGGAAGCCGCCGTGCCCGTGCAGCTTTCCGATGCCGAGACGGT
CGCGCGTCCGCCGTACTGGGACAAGGCGTGCGCCGATCTCGTCAAGCGCGACCGGATTCTCAAGAAGCTGATCCCGAAGT
TCGGCCCCGCGCATCTCGTCAAGCGCGGCGATTCGTTCGTCACGCTCGCGCGCTCGGTGGTCGGCCAGCAGATCTCGGTG
GCCGCCGCGCAGTCGGTCTGGGTCAAGATCGAGACCGCGTGCCCGAAGCTCGCGCCGCCGCAGATCATCAAGCTCGGCCA
GGAAAAACTGATCGCGTGCGGGCTGTCGAAACGCAAATCCGAATATATTCTCGATCTCGCCCAGCATTTCGTGTCGGGCG
CGCTTCACGTCGACAAATGGGCGTCGATGGACGACGAGGACGTGATCGCCGAGCTCACGCAGATCCGAGGGATCGGCCGC
TGGACAGCGGAGATGTTCCTGATCTTCAATTTGTCACGGCCGGACGTGCTGCCGCTCGACGATCTGGGGCTCATTCGCGC
GATCAGCGTCAATTATTTCAGCGGCGAGCCCGTCACGCGCAGCGAGGCGCGCGAAGTGGCGGCGAACTGGGAGCCGTGGC
GGACGGTCGCCACCTGGTACATGTGGCGCAGTCTCGATCCGCTGACCGCTAATAGCTGA

Upstream 100 bases:

>100_bases
GAATCGCACTTGAAGACAAACCGGGCGGATCGACGGAGTGGCGCCGCGTATGAGCGCGCATCGTCTGTCCCGCTGATCGA
TTCGCCAGGTAGGAGGCTAG

Downstream 100 bases:

>100_bases
TCGGAGCTATTGCTTTTCGCTAATCGATAGTTGCGGCCCGGTCTTGCGCCCGGCGGGCGCGGTTAGAATACGCGCTGCCG
CAAGATTCAAGGATTTCAAA

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MATARKAPAKRAASQPPMAAKRAGARAPAVHKAGAKRAAPNGAANGAAHQPGLRAAAGKPARAKVDGERAVDALPREAAA
VAAEPVARKARVSEAAVPVQLSDAETVARPPYWDKACADLVKRDRILKKLIPKFGPAHLVKRGDSFVTLARSVVGQQISV
AAAQSVWVKIETACPKLAPPQIIKLGQEKLIACGLSKRKSEYILDLAQHFVSGALHVDKWASMDDEDVIAELTQIRGIGR
WTAEMFLIFNLSRPDVLPLDDLGLIRAISVNYFSGEPVTRSEAREVAANWEPWRTVATWYMWRSLDPLTANS

Sequences:

>Translated_312_residues
MATARKAPAKRAASQPPMAAKRAGARAPAVHKAGAKRAAPNGAANGAAHQPGLRAAAGKPARAKVDGERAVDALPREAAA
VAAEPVARKARVSEAAVPVQLSDAETVARPPYWDKACADLVKRDRILKKLIPKFGPAHLVKRGDSFVTLARSVVGQQISV
AAAQSVWVKIETACPKLAPPQIIKLGQEKLIACGLSKRKSEYILDLAQHFVSGALHVDKWASMDDEDVIAELTQIRGIGR
WTAEMFLIFNLSRPDVLPLDDLGLIRAISVNYFSGEPVTRSEAREVAANWEPWRTVATWYMWRSLDPLTANS
>Mature_311_residues
ATARKAPAKRAASQPPMAAKRAGARAPAVHKAGAKRAAPNGAANGAAHQPGLRAAAGKPARAKVDGERAVDALPREAAAV
AAEPVARKARVSEAAVPVQLSDAETVARPPYWDKACADLVKRDRILKKLIPKFGPAHLVKRGDSFVTLARSVVGQQISVA
AAQSVWVKIETACPKLAPPQIIKLGQEKLIACGLSKRKSEYILDLAQHFVSGALHVDKWASMDDEDVIAELTQIRGIGRW
TAEMFLIFNLSRPDVLPLDDLGLIRAISVNYFSGEPVTRSEAREVAANWEPWRTVATWYMWRSLDPLTANS

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320990, Length=197, Percent_Identity=31.9796954314721, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012904 [H]

Pfam domain/function: PF00730 HhH-GPD; PF07934 OGG_N [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 33605; Mature: 33474

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATARKAPAKRAASQPPMAAKRAGARAPAVHKAGAKRAAPNGAANGAAHQPGLRAAAGKP
CCCCCCCCHHHHCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCC
ARAKVDGERAVDALPREAAAVAAEPVARKARVSEAAVPVQLSDAETVARPPYWDKACADL
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCCCCHHHHHHHH
VKRDRILKKLIPKFGPAHLVKRGDSFVTLARSVVGQQISVAAAQSVWVKIETACPKLAPP
HHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCH
QIIKLGQEKLIACGLSKRKSEYILDLAQHFVSGALHVDKWASMDDEDVIAELTQIRGIGR
HHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCHH
WTAEMFLIFNLSRPDVLPLDDLGLIRAISVNYFSGEPVTRSEAREVAANWEPWRTVATWY
HHEEEEEEEECCCCCCCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHH
MWRSLDPLTANS
HHHCCCCCCCCC
>Mature Secondary Structure 
ATARKAPAKRAASQPPMAAKRAGARAPAVHKAGAKRAAPNGAANGAAHQPGLRAAAGKP
CCCCCCCHHHHCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCC
ARAKVDGERAVDALPREAAAVAAEPVARKARVSEAAVPVQLSDAETVARPPYWDKACADL
CCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCCCCHHHHHHHH
VKRDRILKKLIPKFGPAHLVKRGDSFVTLARSVVGQQISVAAAQSVWVKIETACPKLAPP
HHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCH
QIIKLGQEKLIACGLSKRKSEYILDLAQHFVSGALHVDKWASMDDEDVIAELTQIRGIGR
HHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHCCHH
WTAEMFLIFNLSRPDVLPLDDLGLIRAISVNYFSGEPVTRSEAREVAANWEPWRTVATWY
HHEEEEEEEECCCCCCCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHH
MWRSLDPLTANS
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376346; 9384377 [H]