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The map label for this gene is tilS [H]

Identifier: 237812903

GI number: 237812903

Start: 2606589

End: 2608058

Strand: Reverse

Name: tilS [H]

Synonym: GBP346_A2663

Alternate gene names: 237812903

Gene position: 2608058-2606589 (Counterclockwise)

Preceding gene: 237812904

Following gene: 237812901

Centisome position: 63.63

GC content: 72.45

Gene sequence:

>1470_bases
GTGATTCCCCCGCATGAATTTTCCGCCGAGCGCGTCGTGTTCGACGCGCTCGGCGTCGCGCTGTCCGCGTTGCCCGACGA
CACGCCGATCGCGATCGCGTATAGCGGCGGCCTCGATTCGACCGTGCTGTTGCACGCGGCCGCGCGCATTGCGGGCGCCG
GACGTTGCATCGCGTTGCATGTTCACCACGGCCTGAGCGCGAACGCCGACGCATGGCTCGCGCATTGCGCGGAAACGGCG
CAAGCGCTCGGCGCGCGTTTCGACGCGGCGCGCGTCGACGTGCCGCGCGCGAGCGGGCAGGGCATCGAGGCGAGCGCGCG
CGATGCGCGCTATCGCGCGCTCGAGACGATGTGCGCGCGATACGGCGCGCGCACGCTGTGGCTCGCGCAGCATGCGGACG
ATCAGGCGGAGACCGTGCTGCTGCAACTGCTGCGCGGCGCGGGCATCGCGGGGTTGGCGGCGATGGCGCCGCAGTACCGG
CCGGCGCTTGCCGACGTCGTGCGCATGCGTCCGCTCCTGCATCTGTTGCGCGCGCAGCTCGAGCGCTACGCGCAGCAGCA
TGCGCTGCGCTGGATCGACGACGAATCGAACACGGATACGCGCTATGCGCGCAATGCGCTGCGCGTCGACGTGCTTCCCG
CGCTTGCGCCGCACTTTCCCGGCTTTCGCGATGCGCTCGCCCGCACCGCGCAGCATGCGGCGGCGGCCCAGCGTCTGCTC
GACGATCTCGCCGCGATCGATCTGCGCGCGGTCGCGCGCGCGGACGTGCGCGTGTTGTCGCGCGATGCGTTCGTCGCGCT
CGACGACGAGCGCGGGGCGAACTTGCTGCGTTACTGGATGCGCTCGCTCGGGCTGCCGGGCGCGTCGGCCGCGCGCCTTG
CCGAGATGGTGAAGCAGTTGCGCGCGGCGCGCGATGCGCATGCGTTGCGCGTCGATCATGCGGGTTGGCGCCTGCGTCTT
TATCGCGACGACGTCCAGTGGGAGGCGGGCGACGGTGCCGCATCGGAGGCGGCGCGCGCCGATGTGGCTGACGATGATGC
GGCCGATGCGCGCGACGACAGGGCCGATGCATCGGCCGCCGCGCGGCTTCCGGCATGCGCGCTCGCCTGGCGGGGGCACG
AGGTGTGGCGTCTGCCCGGCTGGCGCGGCAGTTTCGTGTTCAGTCCGGTCGCGGCGCACGAGCACGATGCGGTGCCGGAG
GCGCTGCTCTCGTCAGCCGCGCTGCGCGCCTGCGCGCGCGCGGGCGGCGAGCGCATGCGCACCTGGCGGGGCGGCCCCGG
GCGCACGCTGAAGAATCTGTTCCAGGAGCGCGGCGTGCCCGCCTGGCAGCGCGATGTGCCGCTGTTGTACGTCGGCGAGC
GGTTGCTGTTCGTGCCGCGCATCGGCGTGAATCGGGCGACGCACGACGGCGCCGATGCGCCGGGCGGCTGGCGGCGCATC
GAATGGCGGCCCGACATGTTGATCGCGTAA

Upstream 100 bases:

>100_bases
CGCTTCGAGCGCTTGATGGCCTACGGCAAGTTCAAGGAAACGACGCCGCGCGCATGAGCGCCGCGCCGTCGAGTCGCGAG
GCGCGTCGCGCCTTCCCGCA

Downstream 100 bases:

>100_bases
GCGCTCCGTCCGGCGCGCGTCGCGGCGGCCACGAATCCCGGGGGCGCGGAAAAAGGTCGCGACCCGGCCTGCCGGCGCCG
TCGCGGCTTGTCAAGCAGCC

Product: tRNA(Ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MIPPHEFSAERVVFDALGVALSALPDDTPIAIAYSGGLDSTVLLHAAARIAGAGRCIALHVHHGLSANADAWLAHCAETA
QALGARFDAARVDVPRASGQGIEASARDARYRALETMCARYGARTLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPQYR
PALADVVRMRPLLHLLRAQLERYAQQHALRWIDDESNTDTRYARNALRVDVLPALAPHFPGFRDALARTAQHAAAAQRLL
DDLAAIDLRAVARADVRVLSRDAFVALDDERGANLLRYWMRSLGLPGASAARLAEMVKQLRAARDAHALRVDHAGWRLRL
YRDDVQWEAGDGAASEAARADVADDDAADARDDRADASAAARLPACALAWRGHEVWRLPGWRGSFVFSPVAAHEHDAVPE
ALLSSAALRACARAGGERMRTWRGGPGRTLKNLFQERGVPAWQRDVPLLYVGERLLFVPRIGVNRATHDGADAPGGWRRI
EWRPDMLIA

Sequences:

>Translated_489_residues
MIPPHEFSAERVVFDALGVALSALPDDTPIAIAYSGGLDSTVLLHAAARIAGAGRCIALHVHHGLSANADAWLAHCAETA
QALGARFDAARVDVPRASGQGIEASARDARYRALETMCARYGARTLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPQYR
PALADVVRMRPLLHLLRAQLERYAQQHALRWIDDESNTDTRYARNALRVDVLPALAPHFPGFRDALARTAQHAAAAQRLL
DDLAAIDLRAVARADVRVLSRDAFVALDDERGANLLRYWMRSLGLPGASAARLAEMVKQLRAARDAHALRVDHAGWRLRL
YRDDVQWEAGDGAASEAARADVADDDAADARDDRADASAAARLPACALAWRGHEVWRLPGWRGSFVFSPVAAHEHDAVPE
ALLSSAALRACARAGGERMRTWRGGPGRTLKNLFQERGVPAWQRDVPLLYVGERLLFVPRIGVNRATHDGADAPGGWRRI
EWRPDMLIA
>Mature_489_residues
MIPPHEFSAERVVFDALGVALSALPDDTPIAIAYSGGLDSTVLLHAAARIAGAGRCIALHVHHGLSANADAWLAHCAETA
QALGARFDAARVDVPRASGQGIEASARDARYRALETMCARYGARTLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPQYR
PALADVVRMRPLLHLLRAQLERYAQQHALRWIDDESNTDTRYARNALRVDVLPALAPHFPGFRDALARTAQHAAAAQRLL
DDLAAIDLRAVARADVRVLSRDAFVALDDERGANLLRYWMRSLGLPGASAARLAEMVKQLRAARDAHALRVDHAGWRLRL
YRDDVQWEAGDGAASEAARADVADDDAADARDDRADASAAARLPACALAWRGHEVWRLPGWRGSFVFSPVAAHEHDAVPE
ALLSSAALRACARAGGERMRTWRGGPGRTLKNLFQERGVPAWQRDVPLLYVGERLLFVPRIGVNRATHDGADAPGGWRRI
EWRPDMLIA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=458, Percent_Identity=34.061135371179, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 53229; Mature: 53229

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPPHEFSAERVVFDALGVALSALPDDTPIAIAYSGGLDSTVLLHAAARIAGAGRCIALH
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
VHHGLSANADAWLAHCAETAQALGARFDAARVDVPRASGQGIEASARDARYRALETMCAR
EECCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
YGARTLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPQYRPALADVVRMRPLLHLLRAQL
HCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ERYAQQHALRWIDDESNTDTRYARNALRVDVLPALAPHFPGFRDALARTAQHAAAAQRLL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
DDLAAIDLRAVARADVRVLSRDAFVALDDERGANLLRYWMRSLGLPGASAARLAEMVKQL
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RAARDAHALRVDHAGWRLRLYRDDVQWEAGDGAASEAARADVADDDAADARDDRADASAA
HHHHHHHHEEEECCCCEEEEEECCCCEECCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHH
ARLPACALAWRGHEVWRLPGWRGSFVFSPVAAHEHDAVPEALLSSAALRACARAGGERMR
HHCCHHHHHCCCCEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHH
TWRGGPGRTLKNLFQERGVPAWQRDVPLLYVGERLLFVPRIGVNRATHDGADAPGGWRRI
HCCCCCCHHHHHHHHHCCCCCHHHCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCCEEE
EWRPDMLIA
EECCCCCCC
>Mature Secondary Structure
MIPPHEFSAERVVFDALGVALSALPDDTPIAIAYSGGLDSTVLLHAAARIAGAGRCIALH
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
VHHGLSANADAWLAHCAETAQALGARFDAARVDVPRASGQGIEASARDARYRALETMCAR
EECCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
YGARTLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPQYRPALADVVRMRPLLHLLRAQL
HCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ERYAQQHALRWIDDESNTDTRYARNALRVDVLPALAPHFPGFRDALARTAQHAAAAQRLL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
DDLAAIDLRAVARADVRVLSRDAFVALDDERGANLLRYWMRSLGLPGASAARLAEMVKQL
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RAARDAHALRVDHAGWRLRLYRDDVQWEAGDGAASEAARADVADDDAADARDDRADASAA
HHHHHHHHEEEECCCCEEEEEECCCCEECCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHH
ARLPACALAWRGHEVWRLPGWRGSFVFSPVAAHEHDAVPEALLSSAALRACARAGGERMR
HHCCHHHHHCCCCEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHH
TWRGGPGRTLKNLFQERGVPAWQRDVPLLYVGERLLFVPRIGVNRATHDGADAPGGWRRI
HCCCCCCHHHHHHHHHCCCCCHHHCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCCEEE
EWRPDMLIA
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA