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The map label for this gene is cysD [H]

Identifier: 237812873

GI number: 237812873

Start: 2566362

End: 2567267

Strand: Reverse

Name: cysD [H]

Synonym: GBP346_A2632

Alternate gene names: 237812873

Gene position: 2567267-2566362 (Counterclockwise)

Preceding gene: 237812874

Following gene: 237812872

Centisome position: 62.64

GC content: 68.54

Gene sequence:

>906_bases
ATGGGCCATCTCGACTGGCTCGAGGCCGAGTCGATCCACATCCTGCGCGAGCTCGTCGCGGAATGCAGCAAGCCGGCGCT
GCTGTTCTCGGGCGGCAAGGATTCGGTGGTCGTGCTGCATCTCGCGCTCAAGGCGTTCGGGCTCGGCGCGAACCGCAGGA
CGACGCTGCCGTTTCCGCTCGTGCACATCGACACGGGCCACAACTACGACGAGGTGATCGATTTCCGCGACCGCCGCGCG
AAGCAGATCGGCGCGGAGCTGGTGGTCGGCCACGTCGAGGATTCGATCGCGCGCGGCACGGTGGTGCTGCGCCGCGAGAC
GGATTCGCGCAACGCCGCGCAGGCGGTCACGCTGCTCGAGACGATCGAGCGGCACGGCTACACGGCGATGATCGGCGGGG
CGCGGCGCGACGAGGAGAAGGCGCGGGCGAAGGAGCGGATTTTCTCGTTTCGCGACGAATTCGGCCAGTGGGACCCGAAG
GCGCAGCGCCCGGAGCTGTGGAGCCTGTACAACGCGCGGCTGCACCGGGGCGAACACCTGCGGGTGTTCCCGATCTCGAA
CTGGACGGAGCTCGACGTGTGGCAGTACATCGCGCGCGAGAAGCTGGAGCTGCCGTCGATCTACTACGCGCACCGCCGGG
AGATCGTGCGGCGCAACGGGCTGCTCGTGCCGGTGACGCCGCTCACGCCGATGCGCGAGGGCGAGACGAGCGAGCAGGCG
CTGGTGCGGTTCCGCACGGTGGGGGACATTTCGTGCACGTGCCCGGTCGAGAGCGACGCGGACGACGTGGAGAAGATCAT
CGCGGAGACGGCGGTGACGGAGATCACGGAGCGCGGGGCGACGCGGATGGACGACCAGGCGTCGGAGGCCGCGATGGAGC
AGCGCAAGAAGCAGGGCTATTTCTGA

Upstream 100 bases:

>100_bases
GCCGTCGCGCCGCAGGGGTTCGGAACCGGGGAGCGGAACCATGAACGCAACGCTCGAACCATCTTCGCCGGGCGGGGCCG
CCGGCGCGTCGACGAGCCGG

Downstream 100 bases:

>100_bases
CGCACGCGAGGACACATCCATCATGAGCATCATCGAGAACAACGAAGACCTCGGCGTACTGCGGTTCATCACGGCGGGCA
GCGTCGACGACGGCAAGCGC

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRRTTLPFPLVHIDTGHNYDEVIDFRDRRA
KQIGAELVVGHVEDSIARGTVVLRRETDSRNAAQAVTLLETIERHGYTAMIGGARRDEEKARAKERIFSFRDEFGQWDPK
AQRPELWSLYNARLHRGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHRREIVRRNGLLVPVTPLTPMREGETSEQA
LVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGYF

Sequences:

>Translated_301_residues
MGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRRTTLPFPLVHIDTGHNYDEVIDFRDRRA
KQIGAELVVGHVEDSIARGTVVLRRETDSRNAAQAVTLLETIERHGYTAMIGGARRDEEKARAKERIFSFRDEFGQWDPK
AQRPELWSLYNARLHRGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHRREIVRRNGLLVPVTPLTPMREGETSEQA
LVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGYF
>Mature_300_residues
GHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRRTTLPFPLVHIDTGHNYDEVIDFRDRRAK
QIGAELVVGHVEDSIARGTVVLRRETDSRNAAQAVTLLETIERHGYTAMIGGARRDEEKARAKERIFSFRDEFGQWDPKA
QRPELWSLYNARLHRGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHRREIVRRNGLLVPVTPLTPMREGETSEQAL
VRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=303, Percent_Identity=46.2046204620462, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34276; Mature: 34145

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRRTTLPFPL
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHCCCCCCCCCCCCEE
VHIDTGHNYDEVIDFRDRRAKQIGAELVVGHVEDSIARGTVVLRRETDSRNAAQAVTLLE
EEEECCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCEEEEEECCCCCHHHHHHHHHH
TIERHGYTAMIGGARRDEEKARAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHRGEHL
HHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEE
RVFPISNWTELDVWQYIAREKLELPSIYYAHRREIVRRNGLLVPVTPLTPMREGETSEQA
EEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHH
LVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGY
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
F
C
>Mature Secondary Structure 
GHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRRTTLPFPL
CCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHCCCCCCCCCCCCEE
VHIDTGHNYDEVIDFRDRRAKQIGAELVVGHVEDSIARGTVVLRRETDSRNAAQAVTLLE
EEEECCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCEEEEEECCCCCHHHHHHHHHH
TIERHGYTAMIGGARRDEEKARAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHRGEHL
HHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEE
RVFPISNWTELDVWQYIAREKLELPSIYYAHRREIVRRNGLLVPVTPLTPMREGETSEQA
EEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHH
LVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGY
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]