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The map label for this gene is dmlR [H]

Identifier: 237812843

GI number: 237812843

Start: 2533635

End: 2534618

Strand: Reverse

Name: dmlR [H]

Synonym: GBP346_A2597

Alternate gene names: 237812843

Gene position: 2534618-2533635 (Counterclockwise)

Preceding gene: 237812846

Following gene: 237812841

Centisome position: 61.84

GC content: 68.8

Gene sequence:

>984_bases
TTGATCTTTTACTTTTCGATATCTAATCTGGAGCGATTTCGGATTCGACGAGGCGGCCGGTGCGCGCCGCCCGCATACGG
GATGGATCGCTTCAAACAGATCGAAACGTTCGCCGCGGTTGCGGCGAAAGGCAGCCTGTCGGCGGCCGCGCATGCGGAAG
GGGTCGCGCCCGCGATCATCGGCCGCCGGCTCGACGCGCTCGAGGAGCGGCTCGGCGTGAAGCTGCTTGTGCGCACCACG
CGCAAGCTCACGCTGACGTTCGAAGGCTCGGCGTTCCTCGAGGATTGCCAGCGCATCATCAACGACATGCAGAACGCCGA
GGCGAGCGTGTCCGCGGGCGGCGTGAAGGCGAGCGGGCATTTGCGGGTGTCGGCGCCCGCGGGCTTCGGCCGGCGCCACG
TCGCGCCGCTCGTACCCCCGTTTACGGGCGCGCACCCGGACGTGTCGGTCACGCTCGATCTGTCCGACCGGATGGTCGAC
CTCGTCAACGAGGGGTTCGATTGCGCGGTGCGCCTGGGCGAGCTGCCCGATTCGTCGCTCGTGTCGCTCAAGCTCGGCGA
GAACCGGCGCGTGTGCGTCGCGTCGCCGGCCTATCTCGCGCGCGCCGGCATGCCCGGGACGCTCGACGATCTCGCGCGCC
ACAACTGCCTCGCGCTCGCGGCGAACGCGAACCAGCAGCGCGGCTGGAGCTTCGTCGACGGCGGCAAGGTGGTGTCGATC
CGCGTGTCCGGGACGATGGAGTGCTCGGACGGCGCGGTGCTGCACGAATGGTGCCTCGAGGGCCATGGCCTCGCATGGCG
CTCGTGGTGGGAAGTCGGCGCGGACATCGCGGCGGGCCGGCTCGTCAGCGTGCTCGACGCGTTCGCCGCGCCGCCGGTCG
GCATTCACGCGGTGTTTCCGCAGCGCCGGCACTTGCCGCTCAGGGTCCGGCTCTTTCTCGATTTCCTGAAGCACACGTAC
GAACGCCCCGGCTATTGGGGATGA

Upstream 100 bases:

>100_bases
AGGCGGGCAGGGTGCCGGGCATGACGTTGCGCTCCACGATCACAGGTCGAAATCGATTCTATTAGTGATGGCTTAAACTA
AAAAAGACAGTAATCATCAC

Downstream 100 bases:

>100_bases
CGTTCGCGCGACGGCGCGTGCCATGGCCGCAGGCGCGGCGTTTCCGCGCGCCGCTCCGGCTGGCGTCTTGCGCGCCGTCG
AATTTGCGCTCCAATGCGTA

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MIFYFSISNLERFRIRRGGRCAPPAYGMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTT
RKLTLTFEGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPPFTGAHPDVSVTLDLSDRMVD
LVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARAGMPGTLDDLARHNCLALAANANQQRGWSFVDGGKVVSI
RVSGTMECSDGAVLHEWCLEGHGLAWRSWWEVGADIAAGRLVSVLDAFAAPPVGIHAVFPQRRHLPLRVRLFLDFLKHTY
ERPGYWG

Sequences:

>Translated_327_residues
MIFYFSISNLERFRIRRGGRCAPPAYGMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTT
RKLTLTFEGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPPFTGAHPDVSVTLDLSDRMVD
LVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARAGMPGTLDDLARHNCLALAANANQQRGWSFVDGGKVVSI
RVSGTMECSDGAVLHEWCLEGHGLAWRSWWEVGADIAAGRLVSVLDAFAAPPVGIHAVFPQRRHLPLRVRLFLDFLKHTY
ERPGYWG
>Mature_327_residues
MIFYFSISNLERFRIRRGGRCAPPAYGMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTT
RKLTLTFEGSAFLEDCQRIINDMQNAEASVSAGGVKASGHLRVSAPAGFGRRHVAPLVPPFTGAHPDVSVTLDLSDRMVD
LVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLARAGMPGTLDDLARHNCLALAANANQQRGWSFVDGGKVVSI
RVSGTMECSDGAVLHEWCLEGHGLAWRSWWEVGADIAAGRLVSVLDAFAAPPVGIHAVFPQRRHLPLRVRLFLDFLKHTY
ERPGYWG

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=35.5172413793103, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=31.4685314685315, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=33.1081081081081, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=30.6896551724138, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=30.0353356890459, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=29.616724738676, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=28.4280936454849, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789173, Length=250, Percent_Identity=28.4, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790011, Length=297, Percent_Identity=24.5791245791246, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786448, Length=279, Percent_Identity=26.1648745519713, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788706, Length=189, Percent_Identity=26.984126984127, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35512; Mature: 35512

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFYFSISNLERFRIRRGGRCAPPAYGMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAII
CEEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHH
GRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAEASVSAGGVKASGH
HHHHHHHHHHHCEEEEEEECEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCE
LRVSAPAGFGRRHVAPLVPPFTGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSL
EEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCE
VSLKLGENRRVCVASPAYLARAGMPGTLDDLARHNCLALAANANQQRGWSFVDGGKVVSI
EEEEECCCCEEEEECCHHHHHCCCCCCHHHHHCCCEEEEEECCCCCCCCEEECCCEEEEE
RVSGTMECSDGAVLHEWCLEGHGLAWRSWWEVGADIAAGRLVSVLDAFAAPPVGIHAVFP
EEECEEECCCCCHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECC
QRRHLPLRVRLFLDFLKHTYERPGYWG
CCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIFYFSISNLERFRIRRGGRCAPPAYGMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAII
CEEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHH
GRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRIINDMQNAEASVSAGGVKASGH
HHHHHHHHHHHCEEEEEEECEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCE
LRVSAPAGFGRRHVAPLVPPFTGAHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSL
EEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCE
VSLKLGENRRVCVASPAYLARAGMPGTLDDLARHNCLALAANANQQRGWSFVDGGKVVSI
EEEEECCCCEEEEECCHHHHHCCCCCCHHHHHCCCEEEEEECCCCCCCCEEECCCEEEEE
RVSGTMECSDGAVLHEWCLEGHGLAWRSWWEVGADIAAGRLVSVLDAFAAPPVGIHAVFP
EEECEEECCCCCHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECC
QRRHLPLRVRLFLDFLKHTYERPGYWG
CCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]