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The map label for this gene is pucI [H]

Identifier: 237812749

GI number: 237812749

Start: 2441102

End: 2442592

Strand: Reverse

Name: pucI [H]

Synonym: GBP346_A2502

Alternate gene names: 237812749

Gene position: 2442592-2441102 (Counterclockwise)

Preceding gene: 237812751

Following gene: 237812748

Centisome position: 59.6

GC content: 65.19

Gene sequence:

>1491_bases
ATGGCTCAGTTCAGTGTGGCGCGGCAAAGCGCCTCGTATCGGCCGAACGAGGATCGCGCCGGCGGCCCGGATGGCGGCGC
TCAGATGCCCGCCGGCTACAGCAGTCGTCTGTACAACGAAGATCTCGCGCCGCTCGCGAGCCAGCGCTGGGGCGCATACA
ACATCTTCGCGTTCTGGATGTCGGACGTGCACAGCGTCGGCGGCTACGTGTTTGCGGGCAGCCTGTTCGCGCTCGGTCTG
ACGAGCTGGCAGGTGCTCGTCGCGCTGATCGTCGGCATTTCGATCGTCAACGTGCTGTGCAACCTGATCGCGAGGCCGAG
CCAGCAGCTAGGCGTGCCGTATCCGGTGGCATGCCGCGCGACGTTCGGCGTGCTCGGCGCGAACGTGCCCGCGGTGATCC
GCGGCCTCATCGCGATCGCATGGTACGGCATCCAAACTTATCTGGCGTCGAGCGCGCTCGCGATCGTCGTGCTCAAGTTC
TTTCCGCACTGGATGCCGTACGCGGACGTGCATCGCTACGGCTTTCTCGGGCTGTCGGCGCTCGGCTGGGCGGGCTTCAT
GCTGCTGTGGGTGCTGCAGGCGTTCGTGTTCTGGAACGGCATGGAGACGATCAAGAAGTTCATCGATTTCGCCGGCCCCG
CCGTCTACGTGGTGATGTTCGCCCTCGCGGGCTACATGGTATGGCGCGCGGGCTGGCGCAATATCGGCCTGAATCTCGGC
GGCGTCCGGTATCACGGCGCCGAAGTGATTCCGGTGATGGTGACGGCGATCTCGCTCGTCGTGTCGTATTTCTCGGGGCC
GATGCTCAACTTCGGCGATTTCTCGCGTTATTGCAGCAGCTACGGCGGCGTGAAGCGCGGCAATTTCTGGGGGCTGCCCG
TCAATTTCCTCGCGTTCTCGCTCGTCACCGTGATCACGACGGCCGCGACGCTGCCGGTGTTCGGAGAACTGATCACCGAT
CCCGTCGAGACGGTCGGGCGCATCGATCATCCGAGCGCCGTGATACTTGGCGCGCTGACCTTCACGATCGCGACGATCGG
CATCAACATCGTCGCGAATTTCGTGTCGCCCGCGTTCGATTTCTCGAACGTCGCGCCGCGCCTGATCAGTTGGCGCGCGG
GCGGGATGCTCGCGGCGGTCGCATCGGTGTTCATCACGCCGTGGAATCTCTTCAACAATCCCGCGGTGATCCATTACACG
CTCGACGTGCTCGGCGGCTTCATCGGGCCGCTGTACGGCGTGCTGATCGTCGATTTCTATCTCGTGAAGCGCGGCGCGCT
GCGGCGCGACGATCTGTACACGACGTCGGCCGACGGCGCGTACTGGTATCGCGACGGCGTGAACCGGCGCGCGATCGCCG
CGCTGTTGCCCGCGGCCGCGATCGCCGTCGCATGCGTGATGGCGCCCGCGCTGTCCGGGCTCGCGAATTTCTCGTGGTTC
ATCGGCGCGGCGCTCGGCGGCGCGTTCTATCGCGCGCTCGCGAAAGCATGA

Upstream 100 bases:

>100_bases
CGTGTGCGGACGCCTCGCCAGGGCGGCATCGGGCGATCCCGTTCGAGCCGCGATTTCAATGACGATCGCGCGGCTCGTCC
GCGGCAGGCAAGGAGATGTC

Downstream 100 bases:

>100_bases
CGGAGTCCCCATGAAAATAAAGCTGATCAATCCGAACACGACGCGGCGGATGACCGACGCGATGGGCCGTTGCGCGCGCG
AGGTCGCGTCGGCGGGCACG

Product: NCS1 nucleoside transporter family protein

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MAQFSVARQSASYRPNEDRAGGPDGGAQMPAGYSSRLYNEDLAPLASQRWGAYNIFAFWMSDVHSVGGYVFAGSLFALGL
TSWQVLVALIVGISIVNVLCNLIARPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALAIVVLKF
FPHWMPYADVHRYGFLGLSALGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFALAGYMVWRAGWRNIGLNLG
GVRYHGAEVIPVMVTAISLVVSYFSGPMLNFGDFSRYCSSYGGVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITD
PVETVGRIDHPSAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITPWNLFNNPAVIHYT
LDVLGGFIGPLYGVLIVDFYLVKRGALRRDDLYTTSADGAYWYRDGVNRRAIAALLPAAAIAVACVMAPALSGLANFSWF
IGAALGGAFYRALAKA

Sequences:

>Translated_496_residues
MAQFSVARQSASYRPNEDRAGGPDGGAQMPAGYSSRLYNEDLAPLASQRWGAYNIFAFWMSDVHSVGGYVFAGSLFALGL
TSWQVLVALIVGISIVNVLCNLIARPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALAIVVLKF
FPHWMPYADVHRYGFLGLSALGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFALAGYMVWRAGWRNIGLNLG
GVRYHGAEVIPVMVTAISLVVSYFSGPMLNFGDFSRYCSSYGGVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITD
PVETVGRIDHPSAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITPWNLFNNPAVIHYT
LDVLGGFIGPLYGVLIVDFYLVKRGALRRDDLYTTSADGAYWYRDGVNRRAIAALLPAAAIAVACVMAPALSGLANFSWF
IGAALGGAFYRALAKA
>Mature_495_residues
AQFSVARQSASYRPNEDRAGGPDGGAQMPAGYSSRLYNEDLAPLASQRWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLT
SWQVLVALIVGISIVNVLCNLIARPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALAIVVLKFF
PHWMPYADVHRYGFLGLSALGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFALAGYMVWRAGWRNIGLNLGG
VRYHGAEVIPVMVTAISLVVSYFSGPMLNFGDFSRYCSSYGGVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDP
VETVGRIDHPSAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASVFITPWNLFNNPAVIHYTL
DVLGGFIGPLYGVLIVDFYLVKRGALRRDDLYTTSADGAYWYRDGVNRRAIAALLPAAAIAVACVMAPALSGLANFSWFI
GAALGGAFYRALAKA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=471, Percent_Identity=28.8747346072187, Blast_Score=190, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319495, Length=485, Percent_Identity=25.5670103092783, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6322219, Length=455, Percent_Identity=23.956043956044, Blast_Score=149, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6319429, Length=472, Percent_Identity=22.8813559322034, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323266, Length=472, Percent_Identity=23.0932203389831, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324766, Length=472, Percent_Identity=22.2457627118644, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324645, Length=468, Percent_Identity=20.9401709401709, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53429; Mature: 53298

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQFSVARQSASYRPNEDRAGGPDGGAQMPAGYSSRLYNEDLAPLASQRWGAYNIFAFWM
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCCHHHHHHHH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HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]