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The map label for this gene is aes [H]

Identifier: 237812645

GI number: 237812645

Start: 2349687

End: 2350646

Strand: Reverse

Name: aes [H]

Synonym: GBP346_A2395

Alternate gene names: 237812645

Gene position: 2350646-2349687 (Counterclockwise)

Preceding gene: 237812646

Following gene: 237812644

Centisome position: 57.35

GC content: 70.1

Gene sequence:

>960_bases
ATGCCGCTGAATCCGAAGATCGCGCAGGTGCTCGACATGATCGAGCGCGCCAAGCGTCCCGATTATCATGAACAGACGCC
CGCGCAGGCGCGCGCCGCGTACGAGAAGAGCGCGCCGATCCTCGACGTCGCGCCCGCGCCGATGTTTTCGGTCGAAGACC
TGCGCGTGCCGTCGCGCGACGGCGGCGCGTTCGGCGCGCGGCTCTATCTGCCCGTCGAGCCGAGTCTCGCCGAGCCGCTG
CCCGTGCTCGTCTACTACCACGGCGGCGGCTTCACGGTCGGCAGCGTGAACACGCACGACGCGCTGTGCCGGATGTTCGC
GCGCGATGCGCAGTGCGCGGTGCTGTCGGTCGATTACCGGCTCGCGCCGGAGCACAAGTTCCCGACCGCCGTCGACGACG
CGGAGGACGCGCTCGTGTGGCTGCATGCGCACGCGTCGCGCTTCGGAATCGATTCGGCGCGGCTCGCAGTGGGCGGCGAC
AGCGCGGGCGGCACGCTCGCGACGGTGTGCGCGGTGCTCGCGCGCGATCGCGGGATCGCGCTCGCGCTGCAACTGCTGAT
CTATCCGGGCACGGTGGGGCATCAGCAGACCGAATCGCACGCGCGGCTCGCGAAAGGCTATCTGTTGTCCGCGGACACGA
TCCAGTGGTTTTTCGGGCATTACGTGCGCGATGCGTCGGATCGCGACGACTGGCGCTTCGCGCCGCTCGACGGCACGCGC
GGCGCGCCGTCGTTCGAGCGCGTCGCGCCCGCGTGGATCGCGACGGCGCAGTACGATCCGCTATCCGACGAAGGCGAAGC
GTATGCGGACAAGCTGCGCGCGGCGGGCAACCGCGTGACGCTCGTCGCGTACGCGGGGATGATTCACGAGTTCTTCAAGA
TGGGCGGCTACGTGCCGGAGGTGCGGCTCGCGCATGCGGACGCGGTGGGCGCGCTGCGCGCGGCGTTCGACGGCGATTGA

Upstream 100 bases:

>100_bases
CGTGGCATCATACGGCGCTTGTCCCGAGCCTTTCACCGGCGCGCCCGCGCCGTTCGCCCGCCGTTTGCGTTGCTGTTTGC
CCCGCCGTTTGCCCTTGACG

Downstream 100 bases:

>100_bases
GCGTGCGCGTGGGCGGGTGACGGCGCACGGTGCGGCGCGGCATGTGCCGGACAGGGCGTGCGGCGACGGCCACGACGAAG
GCGGCGCGGTCCGCCGCGGC

Product: thermophilic carboxylesterase Est2

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSVEDLRVPSRDGGAFGARLYLPVEPSLAEPL
PVLVYYHGGGFTVGSVNTHDALCRMFARDAQCAVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDSARLAVGGD
SAGGTLATVCAVLARDRGIALALQLLIYPGTVGHQQTESHARLAKGYLLSADTIQWFFGHYVRDASDRDDWRFAPLDGTR
GAPSFERVAPAWIATAQYDPLSDEGEAYADKLRAAGNRVTLVAYAGMIHEFFKMGGYVPEVRLAHADAVGALRAAFDGD

Sequences:

>Translated_319_residues
MPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSVEDLRVPSRDGGAFGARLYLPVEPSLAEPL
PVLVYYHGGGFTVGSVNTHDALCRMFARDAQCAVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDSARLAVGGD
SAGGTLATVCAVLARDRGIALALQLLIYPGTVGHQQTESHARLAKGYLLSADTIQWFFGHYVRDASDRDDWRFAPLDGTR
GAPSFERVAPAWIATAQYDPLSDEGEAYADKLRAAGNRVTLVAYAGMIHEFFKMGGYVPEVRLAHADAVGALRAAFDGD
>Mature_318_residues
PLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSVEDLRVPSRDGGAFGARLYLPVEPSLAEPLP
VLVYYHGGGFTVGSVNTHDALCRMFARDAQCAVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDSARLAVGGDS
AGGTLATVCAVLARDRGIALALQLLIYPGTVGHQQTESHARLAKGYLLSADTIQWFFGHYVRDASDRDDWRFAPLDGTRG
APSFERVAPAWIATAQYDPLSDEGEAYADKLRAAGNRVTLVAYAGMIHEFFKMGGYVPEVRLAHADAVGALRAAFDGD

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=273, Percent_Identity=27.8388278388278, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157041239, Length=110, Percent_Identity=40, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI61966717, Length=217, Percent_Identity=27.1889400921659, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI68299767, Length=153, Percent_Identity=35.9477124183007, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI68051721, Length=273, Percent_Identity=26.007326007326, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI21328446, Length=112, Percent_Identity=32.1428571428571, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI226423947, Length=281, Percent_Identity=24.9110320284698, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786682, Length=255, Percent_Identity=31.3725490196078, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI72001146, Length=343, Percent_Identity=25.9475218658892, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996133, Length=291, Percent_Identity=27.1477663230241, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=29.4339622641509, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17540028, Length=148, Percent_Identity=36.4864864864865, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24656084, Length=106, Percent_Identity=35.8490566037736, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24656076, Length=106, Percent_Identity=35.8490566037736, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20130169, Length=106, Percent_Identity=35.8490566037736, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34534; Mature: 34403

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSVEDLRVPSRD
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCC
GGAFGARLYLPVEPSLAEPLPVLVYYHGGGFTVGSVNTHDALCRMFARDAQCAVLSVDYR
CCCCCEEEEEECCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHCCCCEEEEEEEEE
LAPEHKFPTAVDDAEDALVWLHAHASRFGIDSARLAVGGDSAGGTLATVCAVLARDRGIA
ECCCCCCCCCCCCCCCEEEEEEECHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCHH
LALQLLIYPGTVGHQQTESHARLAKGYLLSADTIQWFFGHYVRDASDRDDWRFAPLDGTR
EEEEHHHCCCCCCCCCHHHHHHHHHCEEECHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
GAPSFERVAPAWIATAQYDPLSDEGEAYADKLRAAGNRVTLVAYAGMIHEFFKMGGYVPE
CCCCHHHHCHHHEEECCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCC
VRLAHADAVGALRAAFDGD
EEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSVEDLRVPSRD
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCC
GGAFGARLYLPVEPSLAEPLPVLVYYHGGGFTVGSVNTHDALCRMFARDAQCAVLSVDYR
CCCCCEEEEEECCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHCCCCEEEEEEEEE
LAPEHKFPTAVDDAEDALVWLHAHASRFGIDSARLAVGGDSAGGTLATVCAVLARDRGIA
ECCCCCCCCCCCCCCCEEEEEEECHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCHH
LALQLLIYPGTVGHQQTESHARLAKGYLLSADTIQWFFGHYVRDASDRDDWRFAPLDGTR
EEEEHHHCCCCCCCCCHHHHHHHHHCEEECHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
GAPSFERVAPAWIATAQYDPLSDEGEAYADKLRAAGNRVTLVAYAGMIHEFFKMGGYVPE
CCCCHHHHCHHHEEECCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCC
VRLAHADAVGALRAAFDGD
EEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA