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The map label for this gene is holB [H]

Identifier: 237812639

GI number: 237812639

Start: 2344098

End: 2345132

Strand: Reverse

Name: holB [H]

Synonym: GBP346_A2389

Alternate gene names: 237812639

Gene position: 2345132-2344098 (Counterclockwise)

Preceding gene: 237812640

Following gene: 237812638

Centisome position: 57.22

GC content: 73.53

Gene sequence:

>1035_bases
ATGATCTATCCGTGGCAGACCGACGACTGGAACCGCCTGCAGGCGCTGCGCGCGCATTGGCCGCACGCGCTGCTGCTGTA
CGGGCAGGCGGGAATCGGCAAGCTCGCCTTCGCGCGGCACCTCGCGAAGGGCTTCTTGTGCGAGACGCCGCGCGAGAACG
GCGAACCGTGCGGCGCGTGCGCCGCGTGCACGTGGTTCGAGCAGGGCAATCACCCCGATTACCGGATCGTCGTGCCCGAG
GCGCTCGCGGCCGAGCTGCCGGGCGCGGCCGACGAGCCGAAGGCCGCCGACGCCGACGAAGGCGGCAAGAAGGCCAGGAC
GCCGAGCAAGGAAATCAAGATCGAGCAGGTGCGCGCGCTCGTCGACTTCTGCGGGATCGCGTCGCATCGCGGCGGCGCGC
GCGTCGTCGTGCTGTATCCGGCCGAGGCGCTGAACGTCGCGGCCGCGAACGCGCTATTGAAGACGCTCGAGGAGCCGCCG
TCGGGCGTCGTGTTCCTGCTCGCGTCCGCGCGCGTCGACCGGCTGCTGCCGACGATCGTGAGCCGCTGCCGGCAGTGGCC
GATGGCGGTGCCCGCGCCCGACGCGGCCGCCGCGTGGCTCGCGGCGCAGCAGGTGGCGGACGCGAACGCGCTCCTCGCCG
AGGCGGGGGGCGCGCCGCTCGCCGCGCTCGCGCTCGCGAACGACGAGAACCGGCCGCTGCGCGACTGGACGCTCGCGCAG
CTCGCGGCGGGCGCCGCGTGCGATCCGTTCGCGTGCGGCGAGACGCTGCAGAAACTGCCCGTGCCCGTCGTGCTCGGCTG
GCTGCAGCGCTGGCTGTACGATCTGCTCGCCGAGCGCGCGGCGGGCGCGCCGCGCTATTTCCCCGCGCAGCGCGCGGCGC
TCGCGCGCTGCGCGGCGGCGGCCGACGCGAACGCGTTCGCGCGCTTCCTGAAGACGGTCACGCGGCAGCGCGCGGTGGAG
AACCATCCGCTCAGCGCGCGCCTCGTGTTCGAAGAGCTGTTTCTCGGCTATCGGGAACTGTTCGCGTCGCCCTGA

Upstream 100 bases:

>100_bases
CATCGTTTCGCGATCGTCGATTCGAGCGAGTCGATTCCGCAGATCCGCAAGCAGCTCGAAGGCGTGCTCGCCGCACTGTG
ACGAAAGGAAGACGCGAACG

Downstream 100 bases:

>100_bases
CGCACCGCCTTGCCCAACCGTTTCGTTCATCGGATCGCCTTCATGACCCAGCTTGCCTACCGCGACGCCACGCCCGCCGA
TCTGCCGGCGATCGTCGCGA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MIYPWQTDDWNRLQALRAHWPHALLLYGQAGIGKLAFARHLAKGFLCETPRENGEPCGACAACTWFEQGNHPDYRIVVPE
ALAAELPGAADEPKAADADEGGKKARTPSKEIKIEQVRALVDFCGIASHRGGARVVVLYPAEALNVAAANALLKTLEEPP
SGVVFLLASARVDRLLPTIVSRCRQWPMAVPAPDAAAAWLAAQQVADANALLAEAGGAPLAALALANDENRPLRDWTLAQ
LAAGAACDPFACGETLQKLPVPVVLGWLQRWLYDLLAERAAGAPRYFPAQRAALARCAAAADANAFARFLKTVTRQRAVE
NHPLSARLVFEELFLGYRELFASP

Sequences:

>Translated_344_residues
MIYPWQTDDWNRLQALRAHWPHALLLYGQAGIGKLAFARHLAKGFLCETPRENGEPCGACAACTWFEQGNHPDYRIVVPE
ALAAELPGAADEPKAADADEGGKKARTPSKEIKIEQVRALVDFCGIASHRGGARVVVLYPAEALNVAAANALLKTLEEPP
SGVVFLLASARVDRLLPTIVSRCRQWPMAVPAPDAAAAWLAAQQVADANALLAEAGGAPLAALALANDENRPLRDWTLAQ
LAAGAACDPFACGETLQKLPVPVVLGWLQRWLYDLLAERAAGAPRYFPAQRAALARCAAAADANAFARFLKTVTRQRAVE
NHPLSARLVFEELFLGYRELFASP
>Mature_344_residues
MIYPWQTDDWNRLQALRAHWPHALLLYGQAGIGKLAFARHLAKGFLCETPRENGEPCGACAACTWFEQGNHPDYRIVVPE
ALAAELPGAADEPKAADADEGGKKARTPSKEIKIEQVRALVDFCGIASHRGGARVVVLYPAEALNVAAANALLKTLEEPP
SGVVFLLASARVDRLLPTIVSRCRQWPMAVPAPDAAAAWLAAQQVADANALLAEAGGAPLAALALANDENRPLRDWTLAQ
LAAGAACDPFACGETLQKLPVPVVLGWLQRWLYDLLAERAAGAPRYFPAQRAALARCAAAADANAFARFLKTVTRQRAVE
NHPLSARLVFEELFLGYRELFASP

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=290, Percent_Identity=34.1379310344828, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37052; Mature: 37052

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPWQTDDWNRLQALRAHWPHALLLYGQAGIGKLAFARHLAKGFLCETPRENGEPCGAC
CCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHH
AACTWFEQGNHPDYRIVVPEALAAELPGAADEPKAADADEGGKKARTPSKEIKIEQVRAL
HHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VDFCGIASHRGGARVVVLYPAEALNVAAANALLKTLEEPPSGVVFLLASARVDRLLPTIV
HHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
SRCRQWPMAVPAPDAAAAWLAAQQVADANALLAEAGGAPLAALALANDENRPLRDWTLAQ
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHH
LAAGAACDPFACGETLQKLPVPVVLGWLQRWLYDLLAERAAGAPRYFPAQRAALARCAAA
HHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ADANAFARFLKTVTRQRAVENHPLSARLVFEELFLGYRELFASP
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYPWQTDDWNRLQALRAHWPHALLLYGQAGIGKLAFARHLAKGFLCETPRENGEPCGAC
CCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHH
AACTWFEQGNHPDYRIVVPEALAAELPGAADEPKAADADEGGKKARTPSKEIKIEQVRAL
HHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VDFCGIASHRGGARVVVLYPAEALNVAAANALLKTLEEPPSGVVFLLASARVDRLLPTIV
HHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
SRCRQWPMAVPAPDAAAAWLAAQQVADANALLAEAGGAPLAALALANDENRPLRDWTLAQ
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHH
LAAGAACDPFACGETLQKLPVPVVLGWLQRWLYDLLAERAAGAPRYFPAQRAALARCAAA
HHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ADANAFARFLKTVTRQRAVENHPLSARLVFEELFLGYRELFASP
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]