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The map label for this gene is emrY [H]

Identifier: 237812571

GI number: 237812571

Start: 2286280

End: 2287836

Strand: Direct

Name: emrY [H]

Synonym: GBP346_A2321

Alternate gene names: 237812571

Gene position: 2286280-2287836 (Clockwise)

Preceding gene: 237812570

Following gene: 237812572

Centisome position: 55.78

GC content: 67.82

Gene sequence:

>1557_bases
ATGAGTGCCGCGCCGGGCCGACCGCCGCTCTGGAGCGCCGCGAACCTGCGCGGCGATTTCTTTCCATGGGTGCTCGCGAT
CGTCACCGGCCTCGATTACTTCGACAACGCCGCGTTCTCGTTCTTCGCGAGCTACATCGCGGGCGGAATCAACGCGTCGC
CGGACGAGCTCGTGTGGGCGTCGAGCGCTTACGCGGTGACGGCCGTGCTCGGCATCCTGCAGCAGCAATGGTGGGTCGAC
CGGCTCGGTCACCGGCGTTACGTCGCCGGCTGCATGCTGATGTTCTCGCTTGGCGCGATGGCCGCGGCGGCGGCCGACAC
GTCACTGCAGCTCGCGTTCGCGCGCGGCTTTCAGGGCTATTTCATCGGCCCCATGATGGGCGCGTGCCGGATCCTGATCC
AGGTCAGCTTCGCGCCGAAGGATCGCCCGCCCGCGACGCGCGCGTTCCTCATCATGCTGCTGCTCGGCAGCGCGCTCGCG
CCGATCGCGGGCGGCCTGCTCGTCGCGCACTTCACATGGCGCGCGCTGTTCGCCTGCACGGTGCCGGCCGGCATCCTGTT
CGCGGCGCTCGCGTTCGTCGCGCTGCCCGATTCCGGCCACACGCCGCCCGACGAACGCGGCGGCGCGCATTTCTGGCCGT
ACGTGATCTTCGCGCTCGCGCAAGGCGCGCTGCAGATCGTCATGCAGCAGGTGCGCTTCCAGCTCTTCGCCGGCTCGCCG
CTGCTCGTGCTGCTCGCCGCCGGCGGCCTCGCGGCGCTCGCGTGGTTCGGCCATCATCAGTGGCATCATCCGGCGCCGCT
CGTGCGGCTGCACGCGTTTCGCGAGCGCACGTTCCGGGTCGGCCTGCTGCTCTACCTGTTCTATTACTACGAGACGACGG
GCTACAGCTATCTGATCTCCCGCTTCCTCGAAACCGGGCTCGGCTATCCGGTCGAGAACGCCGGGCGGCTCGTCGGCACG
ATGTCGCTGATCTCCGCGAGCGCGCTCTTCGTCTACCTGCGCTACGCGAAGCTTCTCACGCACAAGAAATGGATCATCGT
GCCCGGCTTCGCGCTCGCCGCGTTCGCCGCGCTATGGATGACGCAGATGTCGCCCGAGGTCGGCGAAGCGGCGCTCGTCG
CGCCGCTCCTGATGCGCGGGCTGCTGCTCCTGTTCATCGTGCTGCCCGTCGCGAACCTGACGTTTCGCGTGTTCGCGATC
GACGAGTATTCGCACGGCTACCGGCTGAAGAACATCGTCCGGCAACTGACGATTTCGTTTGCGACCGCCTCCGTCATCAT
CGTCGAGCAGCATCGGCTCGCCGTGCATCAGACGCGGCTTGTCGAGCAGGCGAACGTCTACAATCCGCTGTTCCGGCAAA
CCCTCGACACGCTCACGCGCGGCTTCGCGGCCGCGGGCCACGCGTTCTCCGACGCGCACGCGCTCGCGCTCGTCACCGTG
AGCCGCACCATCGCGCAACAGGCGTCGTTCCTGGCGTCGCTCGACGGCTTCTACTTCCTCGCGGGCGTCGCGCTCTGCGG
CGGCCTGTTCGCCGCCTGGCAAAAAGAGATCGATTGA

Upstream 100 bases:

>100_bases
TCGACTATGTCGAGCGCCGGCTCGCCGGCGAAAACCCCGACGAGCTGATCGCGCAGCGCCGCGCCGCCGCGCGCGCGCGC
AAGACGGGCGGGACAAGCGC

Downstream 100 bases:

>100_bases
GGTACGTTTAGCGCCTGTCGCTCCACGCCCTTTTCCGCCTCGATGCTCTCCAAACTCAACCAATGGCTCGACGCGCGCCG
CCGCGATCGCGCGTTGCGCT

Product: transporter, major facilitator family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSAAPGRPPLWSAANLRGDFFPWVLAIVTGLDYFDNAAFSFFASYIAGGINASPDELVWASSAYAVTAVLGILQQQWWVD
RLGHRRYVAGCMLMFSLGAMAAAAADTSLQLAFARGFQGYFIGPMMGACRILIQVSFAPKDRPPATRAFLIMLLLGSALA
PIAGGLLVAHFTWRALFACTVPAGILFAALAFVALPDSGHTPPDERGGAHFWPYVIFALAQGALQIVMQQVRFQLFAGSP
LLVLLAAGGLAALAWFGHHQWHHPAPLVRLHAFRERTFRVGLLLYLFYYYETTGYSYLISRFLETGLGYPVENAGRLVGT
MSLISASALFVYLRYAKLLTHKKWIIVPGFALAAFAALWMTQMSPEVGEAALVAPLLMRGLLLLFIVLPVANLTFRVFAI
DEYSHGYRLKNIVRQLTISFATASVIIVEQHRLAVHQTRLVEQANVYNPLFRQTLDTLTRGFAAAGHAFSDAHALALVTV
SRTIAQQASFLASLDGFYFLAGVALCGGLFAAWQKEID

Sequences:

>Translated_518_residues
MSAAPGRPPLWSAANLRGDFFPWVLAIVTGLDYFDNAAFSFFASYIAGGINASPDELVWASSAYAVTAVLGILQQQWWVD
RLGHRRYVAGCMLMFSLGAMAAAAADTSLQLAFARGFQGYFIGPMMGACRILIQVSFAPKDRPPATRAFLIMLLLGSALA
PIAGGLLVAHFTWRALFACTVPAGILFAALAFVALPDSGHTPPDERGGAHFWPYVIFALAQGALQIVMQQVRFQLFAGSP
LLVLLAAGGLAALAWFGHHQWHHPAPLVRLHAFRERTFRVGLLLYLFYYYETTGYSYLISRFLETGLGYPVENAGRLVGT
MSLISASALFVYLRYAKLLTHKKWIIVPGFALAAFAALWMTQMSPEVGEAALVAPLLMRGLLLLFIVLPVANLTFRVFAI
DEYSHGYRLKNIVRQLTISFATASVIIVEQHRLAVHQTRLVEQANVYNPLFRQTLDTLTRGFAAAGHAFSDAHALALVTV
SRTIAQQASFLASLDGFYFLAGVALCGGLFAAWQKEID
>Mature_517_residues
SAAPGRPPLWSAANLRGDFFPWVLAIVTGLDYFDNAAFSFFASYIAGGINASPDELVWASSAYAVTAVLGILQQQWWVDR
LGHRRYVAGCMLMFSLGAMAAAAADTSLQLAFARGFQGYFIGPMMGACRILIQVSFAPKDRPPATRAFLIMLLLGSALAP
IAGGLLVAHFTWRALFACTVPAGILFAALAFVALPDSGHTPPDERGGAHFWPYVIFALAQGALQIVMQQVRFQLFAGSPL
LVLLAAGGLAALAWFGHHQWHHPAPLVRLHAFRERTFRVGLLLYLFYYYETTGYSYLISRFLETGLGYPVENAGRLVGTM
SLISASALFVYLRYAKLLTHKKWIIVPGFALAAFAALWMTQMSPEVGEAALVAPLLMRGLLLLFIVLPVANLTFRVFAID
EYSHGYRLKNIVRQLTISFATASVIIVEQHRLAVHQTRLVEQANVYNPLFRQTLDTLTRGFAAAGHAFSDAHALALVTVS
RTIAQQASFLASLDGFYFLAGVALCGGLFAAWQKEID

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=472, Percent_Identity=20.5508474576271, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1790195, Length=435, Percent_Identity=24.5977011494253, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789042, Length=430, Percent_Identity=20.4651162790698, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87081983, Length=301, Percent_Identity=23.9202657807309, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56578; Mature: 56447

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAAPGRPPLWSAANLRGDFFPWVLAIVTGLDYFDNAAFSFFASYIAGGINASPDELVWA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEC
SSAYAVTAVLGILQQQWWVDRLGHRRYVAGCMLMFSLGAMAAAAADTSLQLAFARGFQGY
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCE
FIGPMMGACRILIQVSFAPKDRPPATRAFLIMLLLGSALAPIAGGLLVAHFTWRALFACT
EHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPAGILFAALAFVALPDSGHTPPDERGGAHFWPYVIFALAQGALQIVMQQVRFQLFAGSP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LLVLLAAGGLAALAWFGHHQWHHPAPLVRLHAFRERTFRVGLLLYLFYYYETTGYSYLIS
HHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RFLETGLGYPVENAGRLVGTMSLISASALFVYLRYAKLLTHKKWIIVPGFALAAFAALWM
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHH
TQMSPEVGEAALVAPLLMRGLLLLFIVLPVANLTFRVFAIDEYSHGYRLKNIVRQLTISF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHH
ATASVIIVEQHRLAVHQTRLVEQANVYNPLFRQTLDTLTRGFAAAGHAFSDAHALALVTV
HHHEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SRTIAQQASFLASLDGFYFLAGVALCGGLFAAWQKEID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]