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The map label for this gene is rumA [H]

Identifier: 237812554

GI number: 237812554

Start: 2271256

End: 2272713

Strand: Reverse

Name: rumA [H]

Synonym: GBP346_A2303

Alternate gene names: 237812554

Gene position: 2272713-2271256 (Counterclockwise)

Preceding gene: 237812556

Following gene: 237812552

Centisome position: 55.45

GC content: 66.87

Gene sequence:

>1458_bases
TTGGCGGGCGTTCGTCTACAATCTCGCCTTTCCCCCACAATCGTTCGTCAGGAAAAGCTGGTGTCAGAAGCCGTCCCCCT
CTCCACGCGCCGCGCATCGTCCGCGGGCGACGCGCCCGGTCGCGCCCCGGTTCTCGATATCGATTCGCTCGACATGGAGG
CGCGCGGCGTCGGCCGCGTCGTCGACGAAAACGGCGAGCCCGGCAAGGTGATTTTCGTCGAGGGCGCGTTGCCCGGCGAG
CGCGTCACTTATTCGAGCTTTCGCCGCAAGCCGACCTACGAGCAGGCGCAGGTCGTCGATATTCTGCGCCCGAGCGTGAT
GCGCACGCAGCCGAAATGCGCGTTTTTCGGCACGTGCGGCGGCTGCTCGATGCAGCATCTCGACATGCGCGCGCAGGTCG
CGATCAAGCAGCGCGTGCTCGAGGATAACCTCTGGCATCTCGCGAAGCTGCGCGCCGAGGCGATGTTCGCGCCGATTCAC
GGGCCGTCGTGGGGCTATCGCTATCGTGCGCGCCTCACCGTGCGCCATGTCGCGAAGAAGGGCGGCGTGCTCGTCGGCTT
CCACGAGAAAAAGAGCAGCTACGTGGCCGACATGACGAGCTGCGAAGTGCTGCCGCCGCACGTGTCGGCGATGCTCGTGC
CGCTGCGCCGGCTGGTCGAGCAATTATCGATCCGCGATCGCATGCCGCAGATCGAGCTCGCGGTCGGCGCCCGGGTGACG
GCGCTCGTGCTGCGCGTGCTCGAGCCGATCAACGCGGCCGACGAGGCGCTCCTGCGCGAATTCGCCGACACGCATCGCGT
GCAGTTCTGGCTGCAGCCGAAAGGGCCGGATACGGTCGCGCCGTTCTATCCGCTTGACGCGCAGCTCGATTACACGCTGC
CGGAATTCGGGATCCGGATGCCGTTCAAGCCGACCGATTTCACGCAGGTCAATCACCAGATCAACCGCGTGCTCGTCGGC
CGCGCGCTGCGGCTGCTCGCGCCCGAACGCGGCGATCGCATGCTCGATCTGTTCTGCGGGATCGGCAACTTCACGCTGCC
GCTCGCGCGGCTCGCACGCGAAGTGGTCGGCATCGAGGGCAGCGACGCGCTGACTGCGCGCGCGCTCGAGAATGCGAAGG
AAAACGGCGTCGACGGCCATACGTCGTTCGCGTGCCGCAACCTGTTCGAGGTGACGGCGGACGATCTGCGCGCGCTCGGC
GCATTCGACAAGTTCCTGGTCGATCCGCCGCGCGAAGGCGCACTCGCGGTATCGAAGGCGCTCGCCGAGATCGCGCAAAG
CGGCGCGGGGCCGTTGCCTGCGCGGATCGTGTACGTGTCGTGCAACCCGTCGACGCTCGCGCGAGACGCGGGGCTCCTCG
TGCACGAGGCGGGTTATCGGCTCAAGGGCGCGGGCGTCGTCAACATGTTCCCGCATACCTCGCATGTCGAATCGATCGCG
CTGTTCGAGCGCGATTGA

Upstream 100 bases:

>100_bases
CGTGCGGCGTCGACGGTGCGCGCGAGGTCGACCGTTCCGTTCGCGTCCCGTCCCCATTGGACGTTCCAACTGATGAGTCG
CATCGTCGAATCTCCGTTGC

Downstream 100 bases:

>100_bases
CGCGGGAGCCGGGTGGCGCGTGCATGAAAAAAACCGGCCCGAAGGCCGGTTTTGGGGCAGGGGGCGCCGGGCGGTCGGGC
GCCGGCCTTCAGTTTCAGTT

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MAGVRLQSRLSPTIVRQEKLVSEAVPLSTRRASSAGDAPGRAPVLDIDSLDMEARGVGRVVDENGEPGKVIFVEGALPGE
RVTYSSFRRKPTYEQAQVVDILRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAEAMFAPIH
GPSWGYRYRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDRMPQIELAVGARVT
ALVLRVLEPINAADEALLREFADTHRVQFWLQPKGPDTVAPFYPLDAQLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVG
RALRLLAPERGDRMLDLFCGIGNFTLPLARLAREVVGIEGSDALTARALENAKENGVDGHTSFACRNLFEVTADDLRALG
AFDKFLVDPPREGALAVSKALAEIAQSGAGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIA
LFERD

Sequences:

>Translated_485_residues
MAGVRLQSRLSPTIVRQEKLVSEAVPLSTRRASSAGDAPGRAPVLDIDSLDMEARGVGRVVDENGEPGKVIFVEGALPGE
RVTYSSFRRKPTYEQAQVVDILRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAEAMFAPIH
GPSWGYRYRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDRMPQIELAVGARVT
ALVLRVLEPINAADEALLREFADTHRVQFWLQPKGPDTVAPFYPLDAQLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVG
RALRLLAPERGDRMLDLFCGIGNFTLPLARLAREVVGIEGSDALTARALENAKENGVDGHTSFACRNLFEVTADDLRALG
AFDKFLVDPPREGALAVSKALAEIAQSGAGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIA
LFERD
>Mature_484_residues
AGVRLQSRLSPTIVRQEKLVSEAVPLSTRRASSAGDAPGRAPVLDIDSLDMEARGVGRVVDENGEPGKVIFVEGALPGER
VTYSSFRRKPTYEQAQVVDILRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAEAMFAPIHG
PSWGYRYRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDRMPQIELAVGARVTA
LVLRVLEPINAADEALLREFADTHRVQFWLQPKGPDTVAPFYPLDAQLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGR
ALRLLAPERGDRMLDLFCGIGNFTLPLARLAREVVGIEGSDALTARALENAKENGVDGHTSFACRNLFEVTADDLRALGA
FDKFLVDPPREGALAVSKALAEIAQSGAGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIAL
FERD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI51173878, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=36.5470852017937, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1787083, Length=398, Percent_Identity=24.8743718592965, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790403, Length=357, Percent_Identity=24.9299719887955, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24666579, Length=209, Percent_Identity=32.0574162679426, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 53295; Mature: 53164

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVRLQSRLSPTIVRQEKLVSEAVPLSTRRASSAGDAPGRAPVLDIDSLDMEARGVGRV
CCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCE
VDENGEPGKVIFVEGALPGERVTYSSFRRKPTYEQAQVVDILRPSVMRTQPKCAFFGTCG
ECCCCCCCEEEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHCCHHHCCCCCEEEEECCC
GCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAEAMFAPIHGPSWGYRYRARLTVRHVAKK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEHHHHHHHHH
GGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDRMPQIELAVGARVT
CCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHH
ALVLRVLEPINAADEALLREFADTHRVQFWLQPKGPDTVAPFYPLDAQLDYTLPEFGIRM
HHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCC
PFKPTDFTQVNHQINRVLVGRALRLLAPERGDRMLDLFCGIGNFTLPLARLAREVVGIEG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCC
SDALTARALENAKENGVDGHTSFACRNLFEVTADDLRALGAFDKFLVDPPREGALAVSKA
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LAEIAQSGAGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIA
HHHHHHCCCCCCCEEEEEEECCCHHHHHHCCEEEECCCCEEECCCEEEECCCCCCHHHEE
LFERD
EEECC
>Mature Secondary Structure 
AGVRLQSRLSPTIVRQEKLVSEAVPLSTRRASSAGDAPGRAPVLDIDSLDMEARGVGRV
CCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCE
VDENGEPGKVIFVEGALPGERVTYSSFRRKPTYEQAQVVDILRPSVMRTQPKCAFFGTCG
ECCCCCCCEEEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHCCHHHCCCCCEEEEECCC
GCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAEAMFAPIHGPSWGYRYRARLTVRHVAKK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEHHHHHHHHH
GGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDRMPQIELAVGARVT
CCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHH
ALVLRVLEPINAADEALLREFADTHRVQFWLQPKGPDTVAPFYPLDAQLDYTLPEFGIRM
HHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCC
PFKPTDFTQVNHQINRVLVGRALRLLAPERGDRMLDLFCGIGNFTLPLARLAREVVGIEG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCC
SDALTARALENAKENGVDGHTSFACRNLFEVTADDLRALGAFDKFLVDPPREGALAVSKA
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LAEIAQSGAGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIA
HHHHHHCCCCCCCEEEEEEECCCHHHHHHCCEEEECCCCEEECCCEEEECCCCCCHHHEE
LFERD
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA