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The map label for this gene is oprM [H]

Identifier: 237812487

GI number: 237812487

Start: 2199152

End: 2201056

Strand: Direct

Name: oprM [H]

Synonym: GBP346_A2236

Alternate gene names: 237812487

Gene position: 2199152-2201056 (Clockwise)

Preceding gene: 237812486

Following gene: 237812489

Centisome position: 53.66

GC content: 74.7

Gene sequence:

>1905_bases
ATGCGCGTTTCGATTCCTTCGCTCCCGAGTCTCGCGCCGCGCGCGCTCGCCGCGGCCGCCGCCATCGCGTTCGCGGGCGC
GCTCGCCGGCTGCGCGCTGTTCGCGCCGAGCGACGCGCCGCCCGCGATGCCGTCGCCCGCGCACTACGGCGCGACACCGC
TGCCCGAGCGCACGGCCGCCGCGCAGGGGCTCGCGCAACGCTTTGACGTGGGCGCGCAGCCGGTGCCCGACTGGTGGAAG
CGCTATCGCTCGCCCGCGCTCGACGCGCTCGTCGACGAAGGGCTGCGCAACAGCCCGACGCTCGCCGCCGCCGAAAAGAG
CCTGAGCGCCGCGCGCGAACAGTTGCGCGCGCAGATCGGCAGCTCGCTGCTGCCGACGATCGACGCCGGCGGCCAGGCGA
CGCGCGAGCGCGCGCTCGGCATGCCGGCGTTCGGCCCGCAGACGGTGCTCTACAACGTGTTCGCGGGCCAGTTGCAGGCG
AGCTACACGCTCGATCTGTTCGGCGCCGCGCGTTTCGCGAACCGCGCGCTGTCCAAGCGCGTCGACGTGAATGCGTACCA
GCTCGAATCCGCGCGGCGCGCGCTCGCCGCGAACATCGTGACGGCCGCCGTCACATCGGCCGCGCTCGCCGAGCAGGTCG
CGACGACCGAGCGGCTCGTCGCGCTCGCGCGCGAACAGGCGAGCGACGCGGCCGCGCGCCACCGGCTCGGCGCGGCGTCG
CGCACCGACATGCTGAACACGCGGCAAAGCGCGGCGGCGCTCGAGGCGAGCCTGCCCGCGCTGCGCCAGCAAGCGCAGAG
CGCGCGCCATGCGCTTGCCGTGCTGCTCGGCCGCACGCCGGATGCCGCGCCCGCGAATCTCGCGCTCGCCGATCTGCATC
TGCCGCAGGACGTGCCGGTCGTCGTGCCGTCGACGCTGCTGCAGGCGCGGCCCGACATCCGCGCGGCCGAGGCCGGCTTG
CAGGCTGCGGCGGCCGAGGTCGGCGTCGCGACCGCGCAGATGTTCCCGCAGTTGTCGCTGTCCGCGTCGATGGGCCATGC
GGGCTTCAAGTGGCCGGCGATGCTGTCGGGCGCGGGCGCGATCTGGAGCGTCGGCGCATCGCTCACGCAGCCGCTCTTTC
ACGGCGGCGCGCTGCTCGCGCAGCGCCGCGCGGCGCGCGCGAGCTACGACGCGGCCGTCGACCAGTACAAGCAGACGGTG
CTCGCCGCCTTCCAGGATGTGGCCGACAAGCTCGCCGCGCTCGAGAACGACGCGAGCGCACTCGACGCGTCGAACCGCTC
GGCCGTCGCCGCGCGCGGCGCGTTCGACGACACCGCGGCGCGCGTGCGGCTCGGCGCGCTGCCGCCGTCGGCCGCGCGCG
CGAGCGAGCAGCAGTACCGCAACGCGCGGCTCGACGAGATCCGCGCGACGGGCACGCGGCTTGTCGATACCGCGCGGCTC
TTTCAAGCGATGGGCACGCCGATCGACGGCGGGCCGGATGCGCGGCGTGTGGGCGGTATGAGCGGCTCGAATGATGTGAG
TGATGTGACTGGATCGAGTGGTGTCGGCGGTGCGCACCGCGTGATCGACATACACGAAGCCGGTGCGGCGCAGCGTGCGG
ACGACGCGAAGCGGACCGGCGACGCGCGCATGCTTCGCGCGACCGGCAAGACCGAGGCGGCGCAAGCTTCCGTGACGGCG
AGCGCGGCCGCCGATGCGCCGGCCGCGCCCGCCCCGGCGGCTGCCGCCAGCGGCGCGAACGCGATACGGATACAGGCGCG
AACGCCACCGACCGCCGGCCCGTCCGCCGGCCCGTCCGCCGGCGCGATCGATGCGGCAAGCGCAACCGGCGCGACGAGCA
CGGCCCGCCCGGCAAACGGGCGCGACACGACGGTCGAATCGAACCCAACGCATTCGCCGAGCTAA

Upstream 100 bases:

>100_bases
TCACGCTCTTCTTCCTGCCGGCGCTCTATGCCGCGTGGTTCCGCGTGAAGCCGGACGAGCGCGATCCGGAGCCGCCCGCC
ACGCCTTCCGGAGCCTGACC

Downstream 100 bases:

>100_bases
CCGCGCGCGGCGCAAGCAGCGGCCGTCGCGGATTCGGCATGCGGCGGATCAAGCATGCAGCGCGTCGGGCGCCGTTGCGC
AGCCGACATCGCCGCGCAGG

Product: efflux transporter, outer membrane factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MRVSIPSLPSLAPRALAAAAAIAFAGALAGCALFAPSDAPPAMPSPAHYGATPLPERTAAAQGLAQRFDVGAQPVPDWWK
RYRSPALDALVDEGLRNSPTLAAAEKSLSAAREQLRAQIGSSLLPTIDAGGQATRERALGMPAFGPQTVLYNVFAGQLQA
SYTLDLFGAARFANRALSKRVDVNAYQLESARRALAANIVTAAVTSAALAEQVATTERLVALAREQASDAAARHRLGAAS
RTDMLNTRQSAAALEASLPALRQQAQSARHALAVLLGRTPDAAPANLALADLHLPQDVPVVVPSTLLQARPDIRAAEAGL
QAAAAEVGVATAQMFPQLSLSASMGHAGFKWPAMLSGAGAIWSVGASLTQPLFHGGALLAQRRAARASYDAAVDQYKQTV
LAAFQDVADKLAALENDASALDASNRSAVAARGAFDDTAARVRLGALPPSAARASEQQYRNARLDEIRATGTRLVDTARL
FQAMGTPIDGGPDARRVGGMSGSNDVSDVTGSSGVGGAHRVIDIHEAGAAQRADDAKRTGDARMLRATGKTEAAQASVTA
SAAADAPAAPAPAAAASGANAIRIQARTPPTAGPSAGPSAGAIDAASATGATSTARPANGRDTTVESNPTHSPS

Sequences:

>Translated_634_residues
MRVSIPSLPSLAPRALAAAAAIAFAGALAGCALFAPSDAPPAMPSPAHYGATPLPERTAAAQGLAQRFDVGAQPVPDWWK
RYRSPALDALVDEGLRNSPTLAAAEKSLSAAREQLRAQIGSSLLPTIDAGGQATRERALGMPAFGPQTVLYNVFAGQLQA
SYTLDLFGAARFANRALSKRVDVNAYQLESARRALAANIVTAAVTSAALAEQVATTERLVALAREQASDAAARHRLGAAS
RTDMLNTRQSAAALEASLPALRQQAQSARHALAVLLGRTPDAAPANLALADLHLPQDVPVVVPSTLLQARPDIRAAEAGL
QAAAAEVGVATAQMFPQLSLSASMGHAGFKWPAMLSGAGAIWSVGASLTQPLFHGGALLAQRRAARASYDAAVDQYKQTV
LAAFQDVADKLAALENDASALDASNRSAVAARGAFDDTAARVRLGALPPSAARASEQQYRNARLDEIRATGTRLVDTARL
FQAMGTPIDGGPDARRVGGMSGSNDVSDVTGSSGVGGAHRVIDIHEAGAAQRADDAKRTGDARMLRATGKTEAAQASVTA
SAAADAPAAPAPAAAASGANAIRIQARTPPTAGPSAGPSAGAIDAASATGATSTARPANGRDTTVESNPTHSPS
>Mature_634_residues
MRVSIPSLPSLAPRALAAAAAIAFAGALAGCALFAPSDAPPAMPSPAHYGATPLPERTAAAQGLAQRFDVGAQPVPDWWK
RYRSPALDALVDEGLRNSPTLAAAEKSLSAAREQLRAQIGSSLLPTIDAGGQATRERALGMPAFGPQTVLYNVFAGQLQA
SYTLDLFGAARFANRALSKRVDVNAYQLESARRALAANIVTAAVTSAALAEQVATTERLVALAREQASDAAARHRLGAAS
RTDMLNTRQSAAALEASLPALRQQAQSARHALAVLLGRTPDAAPANLALADLHLPQDVPVVVPSTLLQARPDIRAAEAGL
QAAAAEVGVATAQMFPQLSLSASMGHAGFKWPAMLSGAGAIWSVGASLTQPLFHGGALLAQRRAARASYDAAVDQYKQTV
LAAFQDVADKLAALENDASALDASNRSAVAARGAFDDTAARVRLGALPPSAARASEQQYRNARLDEIRATGTRLVDTARL
FQAMGTPIDGGPDARRVGGMSGSNDVSDVTGSSGVGGAHRVIDIHEAGAAQRADDAKRTGDARMLRATGKTEAAQASVTA
SAAADAPAAPAPAAAASGANAIRIQARTPPTAGPSAGPSAGAIDAASATGATSTARPANGRDTTVESNPTHSPS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=400, Percent_Identity=27, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI2367348, Length=409, Percent_Identity=25.4278728606357, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 64534; Mature: 64534

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00041 HTH_ARAC_FAMILY_1 ; PS00519 HTH_ASNC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSIPSLPSLAPRALAAAAAIAFAGALAGCALFAPSDAPPAMPSPAHYGATPLPERTAA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHH
AQGLAQRFDVGAQPVPDWWKRYRSPALDALVDEGLRNSPTLAAAEKSLSAAREQLRAQIG
HHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
SSLLPTIDAGGQATRERALGMPAFGPQTVLYNVFAGQLQASYTLDLFGAARFANRALSKR
CCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHH
VDVNAYQLESARRALAANIVTAAVTSAALAEQVATTERLVALAREQASDAAARHRLGAAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
RTDMLNTRQSAAALEASLPALRQQAQSARHALAVLLGRTPDAAPANLALADLHLPQDVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCE
VVPSTLLQARPDIRAAEAGLQAAAAEVGVATAQMFPQLSLSASMGHAGFKWPAMLSGAGA
ECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCH
IWSVGASLTQPLFHGGALLAQRRAARASYDAAVDQYKQTVLAAFQDVADKLAALENDASA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LDASNRSAVAARGAFDDTAARVRLGALPPSAARASEQQYRNARLDEIRATGTRLVDTARL
HCCCCCCCEEECCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQAMGTPIDGGPDARRVGGMSGSNDVSDVTGSSGVGGAHRVIDIHEAGAAQRADDAKRTG
HHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCHHCHHHHHCC
DARMLRATGKTEAAQASVTASAAADAPAAPAPAAAASGANAIRIQARTPPTAGPSAGPSA
CHHEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCC
GAIDAASATGATSTARPANGRDTTVESNPTHSPS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVSIPSLPSLAPRALAAAAAIAFAGALAGCALFAPSDAPPAMPSPAHYGATPLPERTAA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHH
AQGLAQRFDVGAQPVPDWWKRYRSPALDALVDEGLRNSPTLAAAEKSLSAAREQLRAQIG
HHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
SSLLPTIDAGGQATRERALGMPAFGPQTVLYNVFAGQLQASYTLDLFGAARFANRALSKR
CCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHH
VDVNAYQLESARRALAANIVTAAVTSAALAEQVATTERLVALAREQASDAAARHRLGAAS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
RTDMLNTRQSAAALEASLPALRQQAQSARHALAVLLGRTPDAAPANLALADLHLPQDVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCE
VVPSTLLQARPDIRAAEAGLQAAAAEVGVATAQMFPQLSLSASMGHAGFKWPAMLSGAGA
ECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCH
IWSVGASLTQPLFHGGALLAQRRAARASYDAAVDQYKQTVLAAFQDVADKLAALENDASA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LDASNRSAVAARGAFDDTAARVRLGALPPSAARASEQQYRNARLDEIRATGTRLVDTARL
HCCCCCCCEEECCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQAMGTPIDGGPDARRVGGMSGSNDVSDVTGSSGVGGAHRVIDIHEAGAAQRADDAKRTG
HHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCHHCHHHHHCC
DARMLRATGKTEAAQASVTASAAADAPAAPAPAAAASGANAIRIQARTPPTAGPSAGPSA
CHHEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCC
GAIDAASATGATSTARPANGRDTTVESNPTHSPS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]