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The map label for this gene is rnfF [H]

Identifier: 237812435

GI number: 237812435

Start: 2153564

End: 2154622

Strand: Reverse

Name: rnfF [H]

Synonym: GBP346_A2183

Alternate gene names: 237812435

Gene position: 2154622-2153564 (Counterclockwise)

Preceding gene: 237812448

Following gene: 237812434

Centisome position: 52.57

GC content: 73.37

Gene sequence:

>1059_bases
TTGCCCGGTTTGAAGAAACTCGTCGGATCGTCGCTCGCGCTCGCCGTCGTCGTCACGCTGATGGCGTGGCTCGCGCTGAG
GTCCCCGCAGGTATACGTGCAGGGCACGTACGTGTTCGGCACGCGCGTGCAGCTCGCGCTCTACGGCGTGCCGCTCGATC
GCGCGCAGCAGGCGACGAACGCGGTGTTCGCGCATTTCCAGGCGATGCAGCGCGGGCTGCATGCGTGGCAGCCGTCGGAA
ATCACGCGCCTGAACCGGAGCATCGCCGCGGGCGAGCCGTTTCGCGCGTCGCCCGCGACGGCCGAGATCCTGCGCGCCGC
GCGCCGGCTGTCGCTCGACACGCAGGGGCTCTTCGAGCCCGGCATCGGGCGGCTCATTCGCCTGTGGGGTTTCCAGTCCG
ATCAGTTCCGCGTTGCGCCGCCGTCGCCCGACGCGGTGCGGCGCGAGCTCGCGCGCGGCGCGCGCATCGCCGATCTCGCG
ATCTCGCCCGACGGCGTCGTCACGAGCGCGAACCGCGCGGTCGCGATCGATCTGGGCGGCTTCGCGAAGGGCTGGGCGCT
CGACGACGCGGCCGCGATCCTCAGGCGGCAGGGCATCGCCAACGCGCTGATCGACGTCGGCGGCAATCTGCTCGCGCTCG
GCAACAAGGGCGGCGCGGCCTGGCGCGTCGGCGTGCAGGACCCGCGCAAGCCCGGCACGCTCGCGACGCTCGAGCTGCGC
GACGGCGAGGCGATCGGCACGAGCGGCGATTACGAGCGCTTCTTTCAGGCGGAGGGCGTGCGCTACTGCCACCTCATCGA
TCCGCGCAGCGGCTTTCCCGCCGTGCAAAGCGAGGCGGTGACCGTGCTCGTCGCGCCCGGCCCGCACGCGGGCGCGCTGT
CCGACGGCGCGAGCAAGCCGCCGTTCATCGCGGGGCGCGCGGCGATGCCGCTCGCGCGCCGGCTCGGCGTGCAGGCCGTG
CTGATCGTCGATGCGCAGGGGCGCGTATGGGCGACCGACGCGATGGCCGCGCGCGTGCGCTTCGCCGATCCGGCGCTGCG
CGCCGCCCGGCTCGACTAA

Upstream 100 bases:

>100_bases
CACTCAGGCGCGCGGCCCGGAAAAACGATAATCGTTCGGTGTCCGGACGTCGTCCGGGTGCAGGATCGCCTCCCGTCAAT
CGTTTTCGGAAATTCCACCG

Downstream 100 bases:

>100_bases
CACGACGGAGCGCCGCCGCGATGACGAACGCAACACATGAACCGCGCGAACCGCATGAGCTGCACGAACCGCGAGAATCG
CACGCTCCCTGCATCGACTT

Product: ApbE family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MPGLKKLVGSSLALAVVVTLMAWLALRSPQVYVQGTYVFGTRVQLALYGVPLDRAQQATNAVFAHFQAMQRGLHAWQPSE
ITRLNRSIAAGEPFRASPATAEILRAARRLSLDTQGLFEPGIGRLIRLWGFQSDQFRVAPPSPDAVRRELARGARIADLA
ISPDGVVTSANRAVAIDLGGFAKGWALDDAAAILRRQGIANALIDVGGNLLALGNKGGAAWRVGVQDPRKPGTLATLELR
DGEAIGTSGDYERFFQAEGVRYCHLIDPRSGFPAVQSEAVTVLVAPGPHAGALSDGASKPPFIAGRAAMPLARRLGVQAV
LIVDAQGRVWATDAMAARVRFADPALRAARLD

Sequences:

>Translated_352_residues
MPGLKKLVGSSLALAVVVTLMAWLALRSPQVYVQGTYVFGTRVQLALYGVPLDRAQQATNAVFAHFQAMQRGLHAWQPSE
ITRLNRSIAAGEPFRASPATAEILRAARRLSLDTQGLFEPGIGRLIRLWGFQSDQFRVAPPSPDAVRRELARGARIADLA
ISPDGVVTSANRAVAIDLGGFAKGWALDDAAAILRRQGIANALIDVGGNLLALGNKGGAAWRVGVQDPRKPGTLATLELR
DGEAIGTSGDYERFFQAEGVRYCHLIDPRSGFPAVQSEAVTVLVAPGPHAGALSDGASKPPFIAGRAAMPLARRLGVQAV
LIVDAQGRVWATDAMAARVRFADPALRAARLD
>Mature_351_residues
PGLKKLVGSSLALAVVVTLMAWLALRSPQVYVQGTYVFGTRVQLALYGVPLDRAQQATNAVFAHFQAMQRGLHAWQPSEI
TRLNRSIAAGEPFRASPATAEILRAARRLSLDTQGLFEPGIGRLIRLWGFQSDQFRVAPPSPDAVRRELARGARIADLAI
SPDGVVTSANRAVAIDLGGFAKGWALDDAAAILRRQGIANALIDVGGNLLALGNKGGAAWRVGVQDPRKPGTLATLELRD
GEAIGTSGDYERFFQAEGVRYCHLIDPRSGFPAVQSEAVTVLVAPGPHAGALSDGASKPPFIAGRAAMPLARRLGVQAVL
IVDAQGRVWATDAMAARVRFADPALRAARLD

Specific function: Required for nitrogen fixation [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rseC family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=310, Percent_Identity=26.4516129032258, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007359
- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE; PF04246 RseC_MucC [H]

EC number: NA

Molecular weight: Translated: 37374; Mature: 37243

Theoretical pI: Translated: 10.98; Mature: 10.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGLKKLVGSSLALAVVVTLMAWLALRSPQVYVQGTYVFGTRVQLALYGVPLDRAQQATN
CCCHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEEEECCCHHHHHHHHH
AVFAHFQAMQRGLHAWQPSEITRLNRSIAAGEPFRASPATAEILRAARRLSLDTQGLFEP
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
GIGRLIRLWGFQSDQFRVAPPSPDAVRRELARGARIADLAISPDGVVTSANRAVAIDLGG
CHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCEEEEEEECCCCCEEECCCEEEEECCC
FAKGWALDDAAAILRRQGIANALIDVGGNLLALGNKGGAAWRVGVQDPRKPGTLATLELR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCCEEEEEEC
DGEAIGTSGDYERFFQAEGVRYCHLIDPRSGFPAVQSEAVTVLVAPGPHAGALSDGASKP
CCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
PFIAGRAAMPLARRLGVQAVLIVDAQGRVWATDAMAARVRFADPALRAARLD
CEEECCHHHHHHHHCCCEEEEEEECCCCEEECHHHHHEEEECCHHHHHHCCC
>Mature Secondary Structure 
PGLKKLVGSSLALAVVVTLMAWLALRSPQVYVQGTYVFGTRVQLALYGVPLDRAQQATN
CCHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEEEEECCCHHHHHHHHH
AVFAHFQAMQRGLHAWQPSEITRLNRSIAAGEPFRASPATAEILRAARRLSLDTQGLFEP
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
GIGRLIRLWGFQSDQFRVAPPSPDAVRRELARGARIADLAISPDGVVTSANRAVAIDLGG
CHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCEEEEEEECCCCCEEECCCEEEEECCC
FAKGWALDDAAAILRRQGIANALIDVGGNLLALGNKGGAAWRVGVQDPRKPGTLATLELR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCCEEEEEEC
DGEAIGTSGDYERFFQAEGVRYCHLIDPRSGFPAVQSEAVTVLVAPGPHAGALSDGASKP
CCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
PFIAGRAAMPLARRLGVQAVLIVDAQGRVWATDAMAARVRFADPALRAARLD
CEEECCHHHHHHHHCCCEEEEEEECCCCEEECHHHHHEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 2071578 [H]