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The map label for this gene is cbaA [H]

Identifier: 237812428

GI number: 237812428

Start: 2145104

End: 2146102

Strand: Direct

Name: cbaA [H]

Synonym: GBP346_A2176

Alternate gene names: 237812428

Gene position: 2145104-2146102 (Clockwise)

Preceding gene: 237812427

Following gene: 237812429

Centisome position: 52.34

GC content: 73.77

Gene sequence:

>999_bases
ATGGATACCATCGTTCCGTTTCTTTCCGATTCCACCGCACGCTGGCTGCCGCTCGCGCTGTCCGAACAGGTGAGCGGCAA
GGCCCCGCTCGCCGTGATGTGCATGGGGCAGGCGATCGTGCTGTTTCGCGACGCGGCCGGCGCCGTGCGCGCGATGGAAG
ACCGCTGCGCGCATCGCCGCGCGCCGCTGTCGCTCGGGCGCGTCACGCCCGAGGGCCGGCTGCAGTGCGGCTATCACGGC
TGGACCTATGACGGCGCGAGCGGCACGTGCGTCGCGATCCCGAATCTGTCGCCTCACGAGCGGGTGCCGGCGCATTACGC
GGCGCGCGCATACGAGACGCTCGAGCGCGACGGCTACGTCTGGGCGCGCGCGGCGCGCGGCGTCGACACCCGCGAGCCGG
CCGCGGCGGCCGCGCGCGCGACGCGCTGCGCGCAGCGATTCGCGGGCTGCGCGACGGTGAGCCTCGCATACGACGACTAT
GTCGCCGCGCTCGCCGACGGCCCGCATTTGCTGATGCGCATCGCGGGCCTGCGCATCACCGATTACGTGATCGCCGATCC
CGAGCCGCGCGGCGGGCGCCTCGCCATGGAGCGCGGCGTCACGTGGGCGGCGCGCCGCCACGATCATCGGTTCGGCGCGC
ACTACCCGTGGACGCTTCGGCTCACGGCGCCGCAAGATGGCGCGCCCGCCTCGGTCGAAATCGACACGCGCGACGGCGCG
CCGATGCTGTGGGCGTCCGTCGCGATCGCGCCCGCGGCCCGCGGCGCGACGAACCTGCTGTGGCGCGGCGGCGTCGCGGC
CGATACCGGCGGCGCCGGCCCCGCGCTGTTTCGCCTGTGGGCGCGGCTGCGCCGCTCGCCGTTCGCGATGCTCGAGCACG
TCGACGGGCACGCACTCGCGAAGCTCGACGCGTCGTGCTCGCGCGCATGGCCGCGCGCGCAACATGCGCGCGTGATCCCG
GTCGAATCCGACCGGTTACCGTGTGAGGGGAGCAGATGA

Upstream 100 bases:

>100_bases
CGCGCCGGCGGGATTTCGCGCGACGCAGGCTCAGGCGGCAGGCGGCGATGCCCGTCTGCCAGGATTGCCGGAGGCATCGC
GCCCGTCGCGATGAATACCC

Downstream 100 bases:

>100_bases
TGGCGACCGTTGACGAAGACGACATCGGCACGGCGAGCGGCCGCGACGAAGGCGACTGGGTGCCCAACCGGTTTTGCTTG
CGCAACGCCTGGTTTCCCCT

Product: Rieske (2Fe-2S) domain protein

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDTIVPFLSDSTARWLPLALSEQVSGKAPLAVMCMGQAIVLFRDAAGAVRAMEDRCAHRRAPLSLGRVTPEGRLQCGYHG
WTYDGASGTCVAIPNLSPHERVPAHYAARAYETLERDGYVWARAARGVDTREPAAAAARATRCAQRFAGCATVSLAYDDY
VAALADGPHLLMRIAGLRITDYVIADPEPRGGRLAMERGVTWAARRHDHRFGAHYPWTLRLTAPQDGAPASVEIDTRDGA
PMLWASVAIAPAARGATNLLWRGGVAADTGGAGPALFRLWARLRRSPFAMLEHVDGHALAKLDASCSRAWPRAQHARVIP
VESDRLPCEGSR

Sequences:

>Translated_332_residues
MDTIVPFLSDSTARWLPLALSEQVSGKAPLAVMCMGQAIVLFRDAAGAVRAMEDRCAHRRAPLSLGRVTPEGRLQCGYHG
WTYDGASGTCVAIPNLSPHERVPAHYAARAYETLERDGYVWARAARGVDTREPAAAAARATRCAQRFAGCATVSLAYDDY
VAALADGPHLLMRIAGLRITDYVIADPEPRGGRLAMERGVTWAARRHDHRFGAHYPWTLRLTAPQDGAPASVEIDTRDGA
PMLWASVAIAPAARGATNLLWRGGVAADTGGAGPALFRLWARLRRSPFAMLEHVDGHALAKLDASCSRAWPRAQHARVIP
VESDRLPCEGSR
>Mature_332_residues
MDTIVPFLSDSTARWLPLALSEQVSGKAPLAVMCMGQAIVLFRDAAGAVRAMEDRCAHRRAPLSLGRVTPEGRLQCGYHG
WTYDGASGTCVAIPNLSPHERVPAHYAARAYETLERDGYVWARAARGVDTREPAAAAARATRCAQRFAGCATVSLAYDDY
VAALADGPHLLMRIAGLRITDYVIADPEPRGGRLAMERGVTWAARRHDHRFGAHYPWTLRLTAPQDGAPASVEIDTRDGA
PMLWASVAIAPAARGATNLLWRGGVAADTGGAGPALFRLWARLRRSPFAMLEHVDGHALAKLDASCSRAWPRAQHARVIP
VESDRLPCEGSR

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881 [H]

Pfam domain/function: PF00355 Rieske [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 35874; Mature: 35874

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTIVPFLSDSTARWLPLALSEQVSGKAPLAVMCMGQAIVLFRDAAGAVRAMEDRCAHRR
CCCCCCCCCCCCCCEEEEEHHHCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHC
APLSLGRVTPEGRLQCGYHGWTYDGASGTCVAIPNLSPHERVPAHYAARAYETLERDGYV
CCCHHCCCCCCCCEEECCCCEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCEE
WARAARGVDTREPAAAAARATRCAQRFAGCATVSLAYDDYVAALADGPHLLMRIAGLRIT
EEEHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCHHHHHHHHCCEEE
DYVIADPEPRGGRLAMERGVTWAARRHDHRFGAHYPWTLRLTAPQDGAPASVEIDTRDGA
EEEEECCCCCCCEEEHHCCCEEEHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCC
PMLWASVAIAPAARGATNLLWRGGVAADTGGAGPALFRLWARLRRSPFAMLEHVDGHALA
CCHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCEE
KLDASCSRAWPRAQHARVIPVESDRLPCEGSR
HHCCHHHHCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MDTIVPFLSDSTARWLPLALSEQVSGKAPLAVMCMGQAIVLFRDAAGAVRAMEDRCAHRR
CCCCCCCCCCCCCCEEEEEHHHCCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHC
APLSLGRVTPEGRLQCGYHGWTYDGASGTCVAIPNLSPHERVPAHYAARAYETLERDGYV
CCCHHCCCCCCCCEEECCCCEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCEE
WARAARGVDTREPAAAAARATRCAQRFAGCATVSLAYDDYVAALADGPHLLMRIAGLRIT
EEEHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCHHHHHHHHCCEEE
DYVIADPEPRGGRLAMERGVTWAARRHDHRFGAHYPWTLRLTAPQDGAPASVEIDTRDGA
EEEEECCCCCCCEEEHHCCCEEEHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCC
PMLWASVAIAPAARGATNLLWRGGVAADTGGAGPALFRLWARLRRSPFAMLEHVDGHALA
CCHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCEE
KLDASCSRAWPRAQHARVIPVESDRLPCEGSR
HHCCHHHHCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704279 [H]