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The map label for this gene is cysK2 [H]

Identifier: 237812299

GI number: 237812299

Start: 1995906

End: 1997033

Strand: Direct

Name: cysK2 [H]

Synonym: GBP346_A2047

Alternate gene names: 237812299

Gene position: 1995906-1997033 (Clockwise)

Preceding gene: 237812298

Following gene: 237812300

Centisome position: 48.7

GC content: 70.57

Gene sequence:

>1128_bases
ATGAACGACATCATGACCCAGACATTCGACAGGCCCGCCATCCCGGTGTACGACAGCGTATCCGGCTCGCTCGACCATCC
CGACCTGATCCGGCTCGCGCCGGGGCTCGTCGCGGCGGCGTTCCGGCTGATGAAGCTCGTGCCCGCGAAGTACATCATCG
AGAACGCGATCGCGAGCGGGCAGTTGAATCCCGGGATGCCGGTGCTCGAGACATCGAGCGGCACGTTCGCGATGGGCATC
GGGATCGTCTGCGCGGAAAAGCGCATTCCGTTTCACATCGTCAGCGACGCGGCGATCGACGAACGGCTGCAGGCGCGCCT
GCGGCAGTTGGGCGGGCGCGTGCAGATCGTCGGGGCGAACGCGACCGGCTCCAACGTCCAGGTGCTGCGGCTCGAAGCGC
TGCAGGAGCGGTTGCGCGAGAACCCCGGCGGCTTCTGGCCGCGGCAGTACGACAATCCGGACAATCAGCACGCGTACCGC
GCGTTCGCCGCGCAACTGATCCGCACGTTCGGCACGAATCTCACGATCGTCGGCACGGTCGGCTCCGGCGCGTCGACGTG
CGGCACGATCCGGGCGCTGCGCGAGGTCGATCCGTCGATTCCGCTCGTCGGCGTCGATACGTTCGGCAGCGTGCTGTTCG
GGCTGCCCGTCGGCCCGCGCGCGCTGCGCGGCCTCGGCAATTCGATCTATCCGAACAACCTCGACCACACGTGCTTCGAC
CAGGTGCACTGGGTCGCGCCCGACGCAGCCTTCGGGAGCACGCGCCGGCTGCACCGGCAACACGGGCTGTATTGCGGGCC
GACGTCCGGCGCGGCGTTCATGGTCGCCGAATGGCTGCGGGCGCAGCGCGACGACGGCAGGACGATCGTGTTCATCGCGC
CCGACGAAGGGCACCGCTACGCCGACACGGTCTACGACGACGCATGGCTGCGCGGGCAAGGGTACGCCGGCGCGCACGCC
GCGCCGGCCGCCGCTCCCGTGCGGGCGGTGAGCCCGAACGCCGCGTCCGGCCCGTGGGCGTACGTCGAATGGGGGCGCCG
CACGTTCGAGCAGGCGAGCGGCCGCCCGCGTCCGGCGGGCAGCGCGCTCGAGCAGATCCGCGACGTCCGGCCCGCGCCCG
TCGCATGA

Upstream 100 bases:

>100_bases
CGCTCGCGCGCGAGTTGCGCGACGCCCGTTGGCCGCGCGCGGTGCGCGGCGCGAGCGTCGATGCGGATACCTATTTGTGG
ATGCTCGAACAATCGATCGC

Downstream 100 bases:

>100_bases
CGCCGCGAACCACGCATCGCGTTCCGATTTTCCTTTGGCGGCCACATTCATCGTGAAGACCTTCGTATTCATCGAAAGCA
ACACCACCGGCACCGGCCGG

Product: cysteine synthase

Products: @CYST01txt*L-cysteine!; acetate; Coenzyme A [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MNDIMTQTFDRPAIPVYDSVSGSLDHPDLIRLAPGLVAAAFRLMKLVPAKYIIENAIASGQLNPGMPVLETSSGTFAMGI
GIVCAEKRIPFHIVSDAAIDERLQARLRQLGGRVQIVGANATGSNVQVLRLEALQERLRENPGGFWPRQYDNPDNQHAYR
AFAAQLIRTFGTNLTIVGTVGSGASTCGTIRALREVDPSIPLVGVDTFGSVLFGLPVGPRALRGLGNSIYPNNLDHTCFD
QVHWVAPDAAFGSTRRLHRQHGLYCGPTSGAAFMVAEWLRAQRDDGRTIVFIAPDEGHRYADTVYDDAWLRGQGYAGAHA
APAAAPVRAVSPNAASGPWAYVEWGRRTFEQASGRPRPAGSALEQIRDVRPAPVA

Sequences:

>Translated_375_residues
MNDIMTQTFDRPAIPVYDSVSGSLDHPDLIRLAPGLVAAAFRLMKLVPAKYIIENAIASGQLNPGMPVLETSSGTFAMGI
GIVCAEKRIPFHIVSDAAIDERLQARLRQLGGRVQIVGANATGSNVQVLRLEALQERLRENPGGFWPRQYDNPDNQHAYR
AFAAQLIRTFGTNLTIVGTVGSGASTCGTIRALREVDPSIPLVGVDTFGSVLFGLPVGPRALRGLGNSIYPNNLDHTCFD
QVHWVAPDAAFGSTRRLHRQHGLYCGPTSGAAFMVAEWLRAQRDDGRTIVFIAPDEGHRYADTVYDDAWLRGQGYAGAHA
APAAAPVRAVSPNAASGPWAYVEWGRRTFEQASGRPRPAGSALEQIRDVRPAPVA
>Mature_375_residues
MNDIMTQTFDRPAIPVYDSVSGSLDHPDLIRLAPGLVAAAFRLMKLVPAKYIIENAIASGQLNPGMPVLETSSGTFAMGI
GIVCAEKRIPFHIVSDAAIDERLQARLRQLGGRVQIVGANATGSNVQVLRLEALQERLRENPGGFWPRQYDNPDNQHAYR
AFAAQLIRTFGTNLTIVGTVGSGASTCGTIRALREVDPSIPLVGVDTFGSVLFGLPVGPRALRGLGNSIYPNNLDHTCFD
QVHWVAPDAAFGSTRRLHRQHGLYCGPTSGAAFMVAEWLRAQRDDGRTIVFIAPDEGHRYADTVYDDAWLRGQGYAGAHA
APAAAPVRAVSPNAASGPWAYVEWGRRTFEQASGRPRPAGSALEQIRDVRPAPVA

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=281, Percent_Identity=28.4697508896797, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI295821200, Length=281, Percent_Identity=28.4697508896797, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI4557415, Length=281, Percent_Identity=28.4697508896797, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1788754, Length=268, Percent_Identity=25.7462686567164, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI2367138, Length=259, Percent_Identity=24.7104247104247, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17562970, Length=267, Percent_Identity=29.9625468164794, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32566674, Length=257, Percent_Identity=29.9610894941634, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17535051, Length=259, Percent_Identity=29.7297297297297, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32566672, Length=209, Percent_Identity=32.0574162679426, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17534315, Length=311, Percent_Identity=27.3311897106109, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17561720, Length=260, Percent_Identity=30, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115535073, Length=255, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=280, Percent_Identity=26.0714285714286, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24643623, Length=272, Percent_Identity=27.2058823529412, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20129101, Length=272, Percent_Identity=27.2058823529412, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47 [C]

Molecular weight: Translated: 40440; Mature: 40440

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIMTQTFDRPAIPVYDSVSGSLDHPDLIRLAPGLVAAAFRLMKLVPAKYIIENAIASG
CCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QLNPGMPVLETSSGTFAMGIGIVCAEKRIPFHIVSDAAIDERLQARLRQLGGRVQIVGAN
CCCCCCCEEECCCCCEEEEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHCCEEEEEECC
ATGSNVQVLRLEALQERLRENPGGFWPRQYDNPDNQHAYRAFAAQLIRTFGTNLTIVGTV
CCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEC
GSGASTCGTIRALREVDPSIPLVGVDTFGSVLFGLPVGPRALRGLGNSIYPNNLDHTCFD
CCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHHH
QVHWVAPDAAFGSTRRLHRQHGLYCGPTSGAAFMVAEWLRAQRDDGRTIVFIAPDEGHRY
HHHEECCCCCCCHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
ADTVYDDAWLRGQGYAGAHAAPAAAPVRAVSPNAASGPWAYVEWGRRTFEQASGRPRPAG
HHHHHCCCEECCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
SALEQIRDVRPAPVA
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MNDIMTQTFDRPAIPVYDSVSGSLDHPDLIRLAPGLVAAAFRLMKLVPAKYIIENAIASG
CCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QLNPGMPVLETSSGTFAMGIGIVCAEKRIPFHIVSDAAIDERLQARLRQLGGRVQIVGAN
CCCCCCCEEECCCCCEEEEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHCCEEEEEECC
ATGSNVQVLRLEALQERLRENPGGFWPRQYDNPDNQHAYRAFAAQLIRTFGTNLTIVGTV
CCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEC
GSGASTCGTIRALREVDPSIPLVGVDTFGSVLFGLPVGPRALRGLGNSIYPNNLDHTCFD
CCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCHHHHH
QVHWVAPDAAFGSTRRLHRQHGLYCGPTSGAAFMVAEWLRAQRDDGRTIVFIAPDEGHRY
HHHEECCCCCCCHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
ADTVYDDAWLRGQGYAGAHAAPAAAPVRAVSPNAASGPWAYVEWGRRTFEQASGRPRPAG
HHHHHCCCEECCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
SALEQIRDVRPAPVA
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.7 {L-Ser}} 0.12 {acetyl-CoA}} 3.6 {S2-}} 0.1 {S2-}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} [C]

Substrates: O-acetyl-L-serine; L-serine; acetyl-CoA; H2S [C]

Specific reaction: O-acetyl-L-serine + H2S = @CYST01.txt*L-cysteine! + acetate L-serine + acetyl-CoA + H2S = L-cysteine + Coenzyme A + acetate [C]

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12368430 [H]