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The map label for this gene is cynX [H]

Identifier: 237812251

GI number: 237812251

Start: 1952275

End: 1953459

Strand: Reverse

Name: cynX [H]

Synonym: GBP346_A1999

Alternate gene names: 237812251

Gene position: 1953459-1952275 (Counterclockwise)

Preceding gene: 237812253

Following gene: 237812250

Centisome position: 47.66

GC content: 75.78

Gene sequence:

>1185_bases
ATGAACCTACTGCTTTCCCCCGCATGGCGGCTCGCCGCCGTCATTCTCGTCGGCCTGAACCTGCGCCCGGCGCTCGCGTC
GATCGGCCCGCTTCTCGACACGATCCAGCGCGCGAGCGGCCTGTCCGACAGCGCGGCGAGCCTGCTCACGACCCTGCCGA
TCCTGCTGATGGGCGCGCTCGGGCTCGTCGCGCCATGGCTCGCGCGCCGCGTCGGCGTGCGCGCGGGCGTCGGGCTCGGC
GTCGCGCTGATCGGGCTCGCGTGCGCGGCGCGCGTCGCGGCGGGCGCGCCCGCCGCGCTGCTCGCCTCCGCGCTGTGCGC
GGGGCTCGGCATCGCGGCCGCGCAGGCGCTGCTGCCCGCGTTCATCAAGACGCAATTCGCCGCGCGCGCGAGCAGCGTGA
TGGGCTTCTATTCGACGTCGATCATGGGCGGCGCGGTGTTCGCGAGCGTCGCCACGCCATTCGCCGCGCACGCGATCGGC
TGGACCTGCGCGCTCGCCGGCTGGGCGCTGCCCGCCGCCGCGGCCGCGCTGCTGTGGCCGCTCGCGACCCGCGCGACGGC
CCGCCCCGCACACGCGCGCCCCGCCGCGCGCGGCGCCCACGGCGCGCGCGCGTGGCTGCTCGCCGCGTTCTTCGGCCTCG
GCACGGGCGCGTACACGCTCGTGCTCGCCTGGCTGCCGCCCTACTACGTGTCGCTCGGCTGGTCGCCCGAAGCCGCGGGC
GGCCTGCTCGGCGGCGTGACGCTCGCGGAGGTCGTCGCGGGGCTCACCGTGTCGGCGCTCGTCGAGCGGCTGCCGGATCG
CCGCCCCGCGCTCTTCACGGCGCTCGTCTCGCTGTTCGCCGGCCTGATCGCGCTGATCGCCGCGCCGCTCGGCCTGGCGT
TGCCCGCGTCGCTGCTGCTCGGCCTCGGGATCGGCGCGCTGTTTCCGCTGTCGCTGATCGTCACGCTCGATCATGCGTCA
AGCGCGGCCGAAGCCGGCGCGCTCACCGGCTTCGTTCAGGGCGTCGGCTATCTGATCGCGGGCCTGTTTCCATTCGTCGC
GGGCGTGATCCGGCAAAGCGTCGCCGATCTGTCGCCGGCCTGGGGCTTCATGGCCGCGGTGTGCATCGCGATGGCCGCGA
TGGCCGCGCGCTTCGCGCCGCCGCGGCCCGCCATCGCGCGAAGCGGCGCTCGAAGCGGCACGTGA

Upstream 100 bases:

>100_bases
ATCTTGACGTAGAGACAAGTCGAATCTATCTTGATGCCGAGACAATTATACGCACTACGCGTGTCCGGCAACCGTCCGGG
TGTGCGCCGTCTTCATACTC

Downstream 100 bases:

>100_bases
GATCGCGGGCGGGGGCGCGCATGGCGCAACGCCCGGCCGCGCGCCGCCCGCGCCGGAACTCGGCCGCGGCGCCGCGGTCC
CTATTGGGCTTGCTCGTCTG

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; Cyanate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNLLLSPAWRLAAVILVGLNLRPALASIGPLLDTIQRASGLSDSAASLLTTLPILLMGALGLVAPWLARRVGVRAGVGLG
VALIGLACAARVAAGAPAALLASALCAGLGIAAAQALLPAFIKTQFAARASSVMGFYSTSIMGGAVFASVATPFAAHAIG
WTCALAGWALPAAAAALLWPLATRATARPAHARPAARGAHGARAWLLAAFFGLGTGAYTLVLAWLPPYYVSLGWSPEAAG
GLLGGVTLAEVVAGLTVSALVERLPDRRPALFTALVSLFAGLIALIAAPLGLALPASLLLGLGIGALFPLSLIVTLDHAS
SAAEAGALTGFVQGVGYLIAGLFPFVAGVIRQSVADLSPAWGFMAAVCIAMAAMAARFAPPRPAIARSGARSGT

Sequences:

>Translated_394_residues
MNLLLSPAWRLAAVILVGLNLRPALASIGPLLDTIQRASGLSDSAASLLTTLPILLMGALGLVAPWLARRVGVRAGVGLG
VALIGLACAARVAAGAPAALLASALCAGLGIAAAQALLPAFIKTQFAARASSVMGFYSTSIMGGAVFASVATPFAAHAIG
WTCALAGWALPAAAAALLWPLATRATARPAHARPAARGAHGARAWLLAAFFGLGTGAYTLVLAWLPPYYVSLGWSPEAAG
GLLGGVTLAEVVAGLTVSALVERLPDRRPALFTALVSLFAGLIALIAAPLGLALPASLLLGLGIGALFPLSLIVTLDHAS
SAAEAGALTGFVQGVGYLIAGLFPFVAGVIRQSVADLSPAWGFMAAVCIAMAAMAARFAPPRPAIARSGARSGT
>Mature_394_residues
MNLLLSPAWRLAAVILVGLNLRPALASIGPLLDTIQRASGLSDSAASLLTTLPILLMGALGLVAPWLARRVGVRAGVGLG
VALIGLACAARVAAGAPAALLASALCAGLGIAAAQALLPAFIKTQFAARASSVMGFYSTSIMGGAVFASVATPFAAHAIG
WTCALAGWALPAAAAALLWPLATRATARPAHARPAARGAHGARAWLLAAFFGLGTGAYTLVLAWLPPYYVSLGWSPEAAG
GLLGGVTLAEVVAGLTVSALVERLPDRRPALFTALVSLFAGLIALIAAPLGLALPASLLLGLGIGALFPLSLIVTLDHAS
SAAEAGALTGFVQGVGYLIAGLFPFVAGVIRQSVADLSPAWGFMAAVCIAMAAMAARFAPPRPAIARSGARSGT

Specific function: This protein is part of an active transport system that transports exogenous cyanate into E.coli cells [H]

COG id: COG2807

COG function: function code P; Cyanate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Cyanate porter (TC 2.A.1.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786536, Length=375, Percent_Identity=38.4, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1788092, Length=360, Percent_Identity=31.6666666666667, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004747
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 39307; Mature: 39307

Theoretical pI: Translated: 11.59; Mature: 11.59

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLLSPAWRLAAVILVGLNLRPALASIGPLLDTIQRASGLSDSAASLLTTLPILLMGAL
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GLVAPWLARRVGVRAGVGLGVALIGLACAARVAAGAPAALLASALCAGLGIAAAQALLPA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FIKTQFAARASSVMGFYSTSIMGGAVFASVATPFAAHAIGWTCALAGWALPAAAAALLWP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATRATARPAHARPAARGAHGARAWLLAAFFGLGTGAYTLVLAWLPPYYVSLGWSPEAAG
HHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHEECCCCCCHHC
GLLGGVTLAEVVAGLTVSALVERLPDRRPALFTALVSLFAGLIALIAAPLGLALPASLLL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGIGALFPLSLIVTLDHASSAAEAGALTGFVQGVGYLIAGLFPFVAGVIRQSVADLSPA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
WGFMAAVCIAMAAMAARFAPPRPAIARSGARSGT
HHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MNLLLSPAWRLAAVILVGLNLRPALASIGPLLDTIQRASGLSDSAASLLTTLPILLMGAL
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GLVAPWLARRVGVRAGVGLGVALIGLACAARVAAGAPAALLASALCAGLGIAAAQALLPA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FIKTQFAARASSVMGFYSTSIMGGAVFASVATPFAAHAIGWTCALAGWALPAAAAALLWP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATRATARPAHARPAARGAHGARAWLLAAFFGLGTGAYTLVLAWLPPYYVSLGWSPEAAG
HHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHEECCCCCCHHC
GLLGGVTLAEVVAGLTVSALVERLPDRRPALFTALVSLFAGLIALIAAPLGLALPASLLL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGIGALFPLSLIVTLDHASSAAEAGALTGFVQGVGYLIAGLFPFVAGVIRQSVADLSPA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
WGFMAAVCIAMAAMAARFAPPRPAIARSGARSGT
HHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; Cyanate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + Cyanate [Periplasm] = Proton [Cytoplasm] + Cyanate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3049588; 9278503 [H]