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The map label for this gene is rlmB [H]

Identifier: 237812095

GI number: 237812095

Start: 1790452

End: 1791195

Strand: Direct

Name: rlmB [H]

Synonym: GBP346_A1839

Alternate gene names: 237812095

Gene position: 1790452-1791195 (Clockwise)

Preceding gene: 237812094

Following gene: 237812096

Centisome position: 43.68

GC content: 70.43

Gene sequence:

>744_bases
ATGTCACGTCTGAAGGTTCTCTACGGTTTTCATGCGGTGACCGCACGCATGCGGCACGATGCGTCGACGGTCGCGGAGGT
GTTCTACGATGCGACGCGCCGCGATCGCCGGATGCAGGATTTCCTGCAGGCGGCGAAGGAGGCGGGGGTGCGCCTCATCG
CCGCCGACGAGACGCGGCTCTGGGGGCTCGCGCATACCGAGCGCCACCAGGGCGTCGTCGCGCGCGTCGAGGATCTGCCG
CTCGCGCAGAACCTCGCCGAGCTGCTCGACGGGATCGCCGGGCCCGCGCTCTTGCTCGTGCTCGACGGCGTGACCGATCC
GCACAACCTCGGCGCATGCCTGCGCGTGGCCGACGCGGCGGGCGCGCACGCGATCATCGCGCCGCGCGACCGCGCGGCCG
GCCTGAACGCGACCGCGGCGAAGGTCGCGAGCGGCGCGGCCGATACGGTGCCGTACATCACGGTGACGAACCTCGCGCGT
GCGCTGCGCGAGCTGAAGGACGCGGGCGTATGGGTGATCGGCACGTCGGACGACGCGACGGCGAGCGTGTACGACACCAA
GCTCGACGGCCCCGTCGCGCTCGTGATGGGCGCGGAAGGCGAGGGAATGCGGCGCCTCACGCGTGATACGTGCGACGAAG
TGATGTTCATTCCGATGGCGGGCCGCGTCGAGAGCCTGAACGTGTCGGTCGCGAGCGGCGTGTGCCTGTACGAGGCGGTG
CGCCAGCGAGGCGCGAAGAAGTAA

Upstream 100 bases:

>100_bases
CGAAACGCCGCGCGCCCCGCCAGGGCGCGCGGCGCTTTCCGTTTTCCATGCGGCGCGTGCGATGCGCGGCCGCGCGTCTT
GTTCTAAGGTTGTTCCAGCC

Downstream 100 bases:

>100_bases
GCGCGCGCGCATGCACTCGTTCGCCGCGGGCTTTGCGGGGGCGGCGCGCGGGGCGGCGTTCGCGATGCCCGGCGGCCGCT
CGGCGGCGGCGTTTGCGGTG

Product: RNA methyltransferase, TrmH family, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSRLKVLYGFHAVTARMRHDASTVAEVFYDATRRDRRMQDFLQAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLP
LAQNLAELLDGIAGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGVWVIGTSDDATASVYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGRVESLNVSVASGVCLYEAV
RQRGAKK

Sequences:

>Translated_247_residues
MSRLKVLYGFHAVTARMRHDASTVAEVFYDATRRDRRMQDFLQAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLP
LAQNLAELLDGIAGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGVWVIGTSDDATASVYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGRVESLNVSVASGVCLYEAV
RQRGAKK
>Mature_246_residues
SRLKVLYGFHAVTARMRHDASTVAEVFYDATRRDRRMQDFLQAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLPL
AQNLAELLDGIAGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLARA
LRELKDAGVWVIGTSDDATASVYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGRVESLNVSVASGVCLYEAVR
QRGAKK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=265, Percent_Identity=27.1698113207547, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=52.2821576763486, Blast_Score=246, Evalue=8e-67,
Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=34.8684210526316, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788935, Length=241, Percent_Identity=29.045643153527, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17555908, Length=254, Percent_Identity=25.5905511811024, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324775, Length=162, Percent_Identity=31.4814814814815, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26358; Mature: 26227

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKVLYGFHAVTARMRHDASTVAEVFYDATRRDRRMQDFLQAAKEAGVRLIAADETRL
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDLPLAQNLAELLDGIAGPALLLVLDGVTDPHNLGACLRVADAA
EECCHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
GAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLARALRELKDAGVWVIGTSDDAT
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCE
ASVYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGRVESLNVSVASGVCLYEAV
EEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCEEEEEEEHHCCHHHHHHH
RQRGAKK
HHHCCCC
>Mature Secondary Structure 
SRLKVLYGFHAVTARMRHDASTVAEVFYDATRRDRRMQDFLQAAKEAGVRLIAADETRL
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDLPLAQNLAELLDGIAGPALLLVLDGVTDPHNLGACLRVADAA
EECCHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
GAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLARALRELKDAGVWVIGTSDDAT
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCE
ASVYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGRVESLNVSVASGVCLYEAV
EEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCEEEEEEEHHCCHHHHHHH
RQRGAKK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]