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The map label for this gene is truB [H]

Identifier: 237812044

GI number: 237812044

Start: 1734494

End: 1735402

Strand: Direct

Name: truB [H]

Synonym: GBP346_A1786

Alternate gene names: 237812044

Gene position: 1734494-1735402 (Clockwise)

Preceding gene: 237812043

Following gene: 237812050

Centisome position: 42.32

GC content: 70.85

Gene sequence:

>909_bases
ATGGCCCGCCGCGCGCTCGATGGCGTGCTGCTGCTCGACAAGCCGGTCGGCCTGTCGAGCAACGACGCGCTGATGCGCGC
GAAACGCCTGTATCAGGCGAAGAAGGCGGGCCACACGGGCACGCTCGATCCGCTCGCGTCGGGACTGCTGCCGCTTTGCT
TCGGCGAAGCGACGAAGTTCTCGCAGGACCTGCTCGAAGCCGACAAGACCTATGAAGCGACGATGCGGCTCGGCGTGCGC
ACGACGACGGGCGACGCCGAAGGCGACGTGCTCGACACGCGCGACGTGAGCTGCGACGAAGCGGCGGTCCGCGCGGCGCT
CGCGCGCTTCGTCGGCGAGATCGTGCAAGTGCCGCCGATGTACTCGGCGCTCAAGCGCGACGGCAAGCCGCTTTACGAAT
ACGCGCGCGCCGGCCAGACGGTCGAGCGCGAAGGGCGCACGGTGACGATCCGCGCGCTCGCGCTCGTGTCGTGCGCGTTG
CCCGACGTCACGTTTCGCGTGACGTGCAGCAAGGGCACGTACGTGCGCACGCTCGCCGAGGATATCGGCGAAGCGCTCGG
TTGCGGCGCGCATCTGACGATGTTGCGGCGCACCGGGGTCGGCCCGCTGACGCTCGAGCACGCGGTGACGCTCGATGCGC
TCGACGCCGCGACGCAGGACGAGCGCGACGCGCGGCTCGCGCCCGTCGATGCATTGCTGTCGACGTTTCCGTGCGTGAAG
CTCGATGCGGCGCTCGCGACGCGCTTTCTGCACGGCCAGCGGCTGAAGCTCTCCGAGCTTGCGGCGCGGCCCGACGCGGA
CGAAGGTGGGCGCGTGCGCGTCTACGATGCCGATGACCGGCTGCTCGGCGTCGCGCGCGCGTCGGAAGGCGTGCTCGCGC
CGGAGCGGCTCGTCGTGACGGGCGCGTGA

Upstream 100 bases:

>100_bases
CGCGCCTCATCGACGAGGCGAACGCGACGCGCGCGAAGGACGATTGAGTATCGCACGAGCCTTGGTGCAGACCATGACGA
CTGTTTCGCCCCGCCCCCGG

Downstream 100 bases:

>100_bases
TCGCCCGAACGCGCGGTGCGGCGATTTGATCCGCGCTCGCGCGGCACGCGATGAGCCGGGCACGGCCGACGGTCGATGCG
GCGGTGATCGCCGATGGCGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MARRALDGVLLLDKPVGLSSNDALMRAKRLYQAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKTYEATMRLGVR
TTTGDAEGDVLDTRDVSCDEAAVRAALARFVGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIRALALVSCAL
PDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGPLTLEHAVTLDALDAATQDERDARLAPVDALLSTFPCVK
LDAALATRFLHGQRLKLSELAARPDADEGGRVRVYDADDRLLGVARASEGVLAPERLVVTGA

Sequences:

>Translated_302_residues
MARRALDGVLLLDKPVGLSSNDALMRAKRLYQAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKTYEATMRLGVR
TTTGDAEGDVLDTRDVSCDEAAVRAALARFVGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIRALALVSCAL
PDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGPLTLEHAVTLDALDAATQDERDARLAPVDALLSTFPCVK
LDAALATRFLHGQRLKLSELAARPDADEGGRVRVYDADDRLLGVARASEGVLAPERLVVTGA
>Mature_301_residues
ARRALDGVLLLDKPVGLSSNDALMRAKRLYQAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADKTYEATMRLGVRT
TTGDAEGDVLDTRDVSCDEAAVRAALARFVGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRTVTIRALALVSCALP
DVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGPLTLEHAVTLDALDAATQDERDARLAPVDALLSTFPCVKL
DAALATRFLHGQRLKLSELAARPDADEGGRVRVYDADDRLLGVARASEGVLAPERLVVTGA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=248, Percent_Identity=35.4838709677419, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI4503337, Length=261, Percent_Identity=28.735632183908, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI215599015, Length=261, Percent_Identity=28.735632183908, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI2367200, Length=308, Percent_Identity=45.4545454545455, Blast_Score=250, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI17553978, Length=273, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=196, Percent_Identity=34.1836734693878, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323204, Length=264, Percent_Identity=29.9242424242424, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281364189, Length=259, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281364187, Length=259, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281364185, Length=259, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281364183, Length=259, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI62471759, Length=259, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17975520, Length=259, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32321; Mature: 32190

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRALDGVLLLDKPVGLSSNDALMRAKRLYQAKKAGHTGTLDPLASGLLPLCFGEATKF
CCCCHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHCCHHHH
SQDLLEADKTYEATMRLGVRTTTGDAEGDVLDTRDVSCDEAAVRAALARFVGEIVQVPPM
HHHHHHHCHHHHHHHHCCEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCH
YSALKRDGKPLYEYARAGQTVEREGRTVTIRALALVSCALPDVTFRVTCSKGTYVRTLAE
HHHHHHCCCHHHHHHHCCCCCHHCCCEEEEHHHHHHHHCCCCEEEEEEECCCCHHHHHHH
DIGEALGCGAHLTMLRRTGVGPLTLEHAVTLDALDAATQDERDARLAPVDALLSTFPCVK
HHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHH
LDAALATRFLHGQRLKLSELAARPDADEGGRVRVYDADDRLLGVARASEGVLAPERLVVT
HHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCCCCCCCEEEE
GA
CC
>Mature Secondary Structure 
ARRALDGVLLLDKPVGLSSNDALMRAKRLYQAKKAGHTGTLDPLASGLLPLCFGEATKF
CCCHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHCCHHHH
SQDLLEADKTYEATMRLGVRTTTGDAEGDVLDTRDVSCDEAAVRAALARFVGEIVQVPPM
HHHHHHHCHHHHHHHHCCEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCH
YSALKRDGKPLYEYARAGQTVEREGRTVTIRALALVSCALPDVTFRVTCSKGTYVRTLAE
HHHHHHCCCHHHHHHHCCCCCHHCCCEEEEHHHHHHHHCCCCEEEEEEECCCCHHHHHHH
DIGEALGCGAHLTMLRRTGVGPLTLEHAVTLDALDAATQDERDARLAPVDALLSTFPCVK
HHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHH
LDAALATRFLHGQRLKLSELAARPDADEGGRVRVYDADDRLLGVARASEGVLAPERLVVT
HHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCCCCCCCEEEE
GA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA