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The map label for this gene is oprM [H]

Identifier: 237811927

GI number: 237811927

Start: 1602335

End: 1604035

Strand: Reverse

Name: oprM [H]

Synonym: GBP346_A1667

Alternate gene names: 237811927

Gene position: 1604035-1602335 (Counterclockwise)

Preceding gene: 237811928

Following gene: 237811916

Centisome position: 39.14

GC content: 74.96

Gene sequence:

>1701_bases
ATGCGGCGCGCGCTCACGCGATGGTGGCTGTCGCTCGCGCTCGGCGCGCTCGCCGCGCTCGCGAGCGCGTGCACGGTCGG
GCCGGACTTTCATCCTCCGCGCGCCGACGTGCCCGCGCAATGGCATGACGTGACGGTTACTTCGGCTTCATCGGCTTCAT
CGGCCGCATCGCCCCCGGCGGCCGGCTCGGTCGCGCCGAACGCCGGCGCGCCGCCCCGTTCGCTGCCGACGGTCGAGGCC
GACCCGGACCCGCGCTGGTGGCGCACGTTCGGCGATCCGGTGCTCGATCGCCTCGTCGAGCGCGCCGCGCAGGACAATCT
CGACGTGCAGGCCGCGGTGCCGCGCATCGCCGAGGCGCGCGCGCAGGTGCGCGCGGCGGCCGCGCAGGGGCTGCCCGACG
TGCGCGCGAGCGCGAGCTACCAGCGCGAACAGCTCGGCCTGAAGGGCTTTCTCGAGGACGGCGGCGTAGACGCGCAGCTC
GAGCGGCTGGGCGCGCCGGGCTCGCCGCTCGAGCGCATCGCGCCCGGCGCGGGCGCGGCGCTGCGCCGCGGCGCCGAGCA
CACGCTCGACCAGCTCACGTCGCCCGTGAACCTGTGGCAGGCCGGCTTCGACGCATCGTGGGAGCTCGACCTGTTCGGCC
GCGTGCGGCGCTCGGTCGAGGCGGCGGGCGCGCAGACGCAAGCCGCGATTGAAGGCCGCAACGACGCGCTGCTGTCGCTC
GAAGCGGAAGTCGCGCAGACCTATCTGCAACTGCGCGGCGCGCAGGCGCTGCGCGCGCTCGCCGTCGCGCTCGCCGACGA
GCAGCGCGACGTCGTCGCGCTCACGCGCGATCAGGCGCGCAAGGGGCTGTCGAGCGAGCTCGACGTGCAAAGCGCCGACG
CGCGACTCGCGCAACTGCGCGCGCAGTTGCCGCAATTCGACCAGCAGATCGCGCTGCTGCGCAACGGCCTGTCCTACCTG
CTCGGCGACGCGCCGGGCGCGCTCGACGACGCGCTGCGCGAGCCGCGCGCGCTGCCGGAGCTTCCGCCGACGGTGCCGAT
CGGGCTGCCGTCGACGCTCGCGCGCCGCCGCCCGGACATCCGTCGCGCGGAAGCGCAACTGCACGCGGCGACCGCCGGCG
TCGGCGTGGCTGTCGCGCAGTTCTACCCGGACGTCTCGCTGACGGGCCAGGTCGGCACGCGCGCGACGCACGCGCGCGAG
CTCGCGCACTGGTCGCATCTGTTCTATGCGTTCGGGCCGAGCGTGTCGCTGCCGATCTTCGAGGGCGGCGCGCTCGTCGC
GAACCTGCGCCTATCGCAGGCGCAACAGGCGCAGGCGGCGCTCGGCTATCGGCGCGCCGTGCTGCTCGCGCTGCGCGACG
TCGACAACGCGCTCGCCGCGTACCGGACCGACCAGACGCGCCGTGACGCGCTGCGTGACGCGGCGGCCGCCGAGCGCACG
ACGTTCGAGCTCGCGCGCGACAGCTATCGCCACGGGCTCGTGTCGTTCATCGACGTGCTCGACGCCGAGCGCGAGCTGTC
GCAGGCGCGCCAGCAATACGTGCAGGGCAGCCTGCAACTGACGACCGATCTCGTCTCGCTGTACAAGGCGCTCGGCGGCG
GCTGGCAGCCCGCGCCGGCCGCGCAAACGCCCGCGCCCGCCGCCCTCGACGCCCCGACGCAAGACACGCCGCTCGGCGCA
ACGGCGCCTGCGCGACGATGA

Upstream 100 bases:

>100_bases
GACGTGTTCGCGATGCTCGCGATCTTCTGCTTCGTGTGCACGCCGCTCACGCTGCTTTTCTCGCCCGTGAAGGCGGCGGG
CGGCCCGGGAGGACACTGAT

Downstream 100 bases:

>100_bases
ACAGCCGCTTGAACGGAAACCGCGTGCGGCCGTCGTCTCCGCGCGGATAGAGCGCATGCAGGCGCGGCACGTATCGATCG
AGACAGCGGTCGCGCTCGTC

Product: efflux transporter, outer membrane factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MRRALTRWWLSLALGALAALASACTVGPDFHPPRADVPAQWHDVTVTSASSASSAASPPAAGSVAPNAGAPPRSLPTVEA
DPDPRWWRTFGDPVLDRLVERAAQDNLDVQAAVPRIAEARAQVRAAAAQGLPDVRASASYQREQLGLKGFLEDGGVDAQL
ERLGAPGSPLERIAPGAGAALRRGAEHTLDQLTSPVNLWQAGFDASWELDLFGRVRRSVEAAGAQTQAAIEGRNDALLSL
EAEVAQTYLQLRGAQALRALAVALADEQRDVVALTRDQARKGLSSELDVQSADARLAQLRAQLPQFDQQIALLRNGLSYL
LGDAPGALDDALREPRALPELPPTVPIGLPSTLARRRPDIRRAEAQLHAATAGVGVAVAQFYPDVSLTGQVGTRATHARE
LAHWSHLFYAFGPSVSLPIFEGGALVANLRLSQAQQAQAALGYRRAVLLALRDVDNALAAYRTDQTRRDALRDAAAAERT
TFELARDSYRHGLVSFIDVLDAERELSQARQQYVQGSLQLTTDLVSLYKALGGGWQPAPAAQTPAPAALDAPTQDTPLGA
TAPARR

Sequences:

>Translated_566_residues
MRRALTRWWLSLALGALAALASACTVGPDFHPPRADVPAQWHDVTVTSASSASSAASPPAAGSVAPNAGAPPRSLPTVEA
DPDPRWWRTFGDPVLDRLVERAAQDNLDVQAAVPRIAEARAQVRAAAAQGLPDVRASASYQREQLGLKGFLEDGGVDAQL
ERLGAPGSPLERIAPGAGAALRRGAEHTLDQLTSPVNLWQAGFDASWELDLFGRVRRSVEAAGAQTQAAIEGRNDALLSL
EAEVAQTYLQLRGAQALRALAVALADEQRDVVALTRDQARKGLSSELDVQSADARLAQLRAQLPQFDQQIALLRNGLSYL
LGDAPGALDDALREPRALPELPPTVPIGLPSTLARRRPDIRRAEAQLHAATAGVGVAVAQFYPDVSLTGQVGTRATHARE
LAHWSHLFYAFGPSVSLPIFEGGALVANLRLSQAQQAQAALGYRRAVLLALRDVDNALAAYRTDQTRRDALRDAAAAERT
TFELARDSYRHGLVSFIDVLDAERELSQARQQYVQGSLQLTTDLVSLYKALGGGWQPAPAAQTPAPAALDAPTQDTPLGA
TAPARR
>Mature_566_residues
MRRALTRWWLSLALGALAALASACTVGPDFHPPRADVPAQWHDVTVTSASSASSAASPPAAGSVAPNAGAPPRSLPTVEA
DPDPRWWRTFGDPVLDRLVERAAQDNLDVQAAVPRIAEARAQVRAAAAQGLPDVRASASYQREQLGLKGFLEDGGVDAQL
ERLGAPGSPLERIAPGAGAALRRGAEHTLDQLTSPVNLWQAGFDASWELDLFGRVRRSVEAAGAQTQAAIEGRNDALLSL
EAEVAQTYLQLRGAQALRALAVALADEQRDVVALTRDQARKGLSSELDVQSADARLAQLRAQLPQFDQQIALLRNGLSYL
LGDAPGALDDALREPRALPELPPTVPIGLPSTLARRRPDIRRAEAQLHAATAGVGVAVAQFYPDVSLTGQVGTRATHARE
LAHWSHLFYAFGPSVSLPIFEGGALVANLRLSQAQQAQAALGYRRAVLLALRDVDNALAAYRTDQTRRDALRDAAAAERT
TFELARDSYRHGLVSFIDVLDAERELSQARQQYVQGSLQLTTDLVSLYKALGGGWQPAPAAQTPAPAALDAPTQDTPLGA
TAPARR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=520, Percent_Identity=27.5, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI2367348, Length=465, Percent_Identity=26.6666666666667, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 60254; Mature: 60254

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRALTRWWLSLALGALAALASACTVGPDFHPPRADVPAQWHDVTVTSASSASSAASPPA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
AGSVAPNAGAPPRSLPTVEADPDPRWWRTFGDPVLDRLVERAAQDNLDVQAAVPRIAEAR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AQVRAAAAQGLPDVRASASYQREQLGLKGFLEDGGVDAQLERLGAPGSPLERIAPGAGAA
HHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHCCCCCCCHHHHCCCCCHH
LRRGAEHTLDQLTSPVNLWQAGFDASWELDLFGRVRRSVEAAGAQTQAAIEGRNDALLSL
HHCCHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEH
EAEVAQTYLQLRGAQALRALAVALADEQRDVVALTRDQARKGLSSELDVQSADARLAQLR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHH
AQLPQFDQQIALLRNGLSYLLGDAPGALDDALREPRALPELPPTVPIGLPSTLARRRPDI
HHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCH
RRAEAQLHAATAGVGVAVAQFYPDVSLTGQVGTRATHARELAHWSHLFYAFGPSVSLPIF
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
EGGALVANLRLSQAQQAQAALGYRRAVLLALRDVDNALAAYRTDQTRRDALRDAAAAERT
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TFELARDSYRHGLVSFIDVLDAERELSQARQQYVQGSLQLTTDLVSLYKALGGGWQPAPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
AQTPAPAALDAPTQDTPLGATAPARR
CCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRALTRWWLSLALGALAALASACTVGPDFHPPRADVPAQWHDVTVTSASSASSAASPPA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
AGSVAPNAGAPPRSLPTVEADPDPRWWRTFGDPVLDRLVERAAQDNLDVQAAVPRIAEAR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AQVRAAAAQGLPDVRASASYQREQLGLKGFLEDGGVDAQLERLGAPGSPLERIAPGAGAA
HHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHCCCCCCCHHHHCCCCCHH
LRRGAEHTLDQLTSPVNLWQAGFDASWELDLFGRVRRSVEAAGAQTQAAIEGRNDALLSL
HHCCHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEH
EAEVAQTYLQLRGAQALRALAVALADEQRDVVALTRDQARKGLSSELDVQSADARLAQLR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHH
AQLPQFDQQIALLRNGLSYLLGDAPGALDDALREPRALPELPPTVPIGLPSTLARRRPDI
HHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCH
RRAEAQLHAATAGVGVAVAQFYPDVSLTGQVGTRATHARELAHWSHLFYAFGPSVSLPIF
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
EGGALVANLRLSQAQQAQAALGYRRAVLLALRDVDNALAAYRTDQTRRDALRDAAAAERT
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TFELARDSYRHGLVSFIDVLDAERELSQARQQYVQGSLQLTTDLVSLYKALGGGWQPAPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
AQTPAPAALDAPTQDTPLGATAPARR
CCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]