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The map label for this gene is pckG [H]

Identifier: 237811740

GI number: 237811740

Start: 1416282

End: 1418132

Strand: Direct

Name: pckG [H]

Synonym: GBP346_A1478

Alternate gene names: 237811740

Gene position: 1416282-1418132 (Clockwise)

Preceding gene: 237811738

Following gene: 237811741

Centisome position: 34.56

GC content: 69.04

Gene sequence:

>1851_bases
GTGGTCGCGGCGACGCGCACCGTTCCGATCGACGTTCCCGAATACGTGAAGCACCGCGGCCTGATCGACTGGGTCGCGCG
TATCGCGGAGTTGACCGAGCCGGATCGCGTTGTCTGGTGCGACGGTTCGCAGCAGGAGTACGACCGCCTCTGCGACGCGA
TGGTCGAACAGCGCACGATGGTGCGCCTCAATCCGGCGAAGCGGCCGAACTCGTTTCTCGCGCTGTCCGATCCGTCCGAC
GTCGCGCGTGTCGAAGACCGCACGTTCATCTGCAGCGAGTATCGCGACGACGCGGGCCCGACCAATCACTGGGTCGCGCC
CGCCGAAATGCGCGCGACGCTCAACGGCCTGTTTCGCGGCGCGATGCGCGGCCGCACGCTGTACGTCGTGCCGTTCTCGA
TGGGCCCGCTCGGCTCGCCGATCGCGCACATCGGCGTCGAGCTCTCGGATAGCCCCTACGTCGTCGTCAACATGCGGATC
ATGACGCGCATGGGGCGCGCGGTGCTCGACGCGCTCGGCGAGCGCGGCGAGTACGTGCCGTGCGTGCACAGCGTCGGGCG
CCCGCTCGCGGCGGGCGAGCAGGATGTGCCGTGGCCGTGCAATCCGACCAAGTACATCGTGCATTTTCCCGAGTCGCGCG
AGATCTGGAGCTTCGGCTCGGGCTACGGCGGCAACGCGCTCCTCGGCAAGAAGTGCTTCGCGCTGCGGATCGCGTCGACG
ATGGGGCGCGACGAAGGCTGGCTCGCCGAGCACATGCTGATCCTCGGCGTGACCTCGCCCGAGGGCCGCAAGTATCACAT
CGCCGCGGCGTTTCCTTCCGCGTGCGGCAAGACCAACTTCGCGATGCTGATCCCGCCGAAGGGCTTCGAGGGCTGGCGCG
TGACGACGATCGGCGACGACATCGCGTGGCTCAAGCCGGGCCGCGACGGGCGGCTGTATGCGATCAACCCGGAGGCGGGC
TATTTCGGCGTCGCGCCGGGCACGGGCGAGAAGACCAATCCGAACGCGCTCGCGACGCTCAGGGAGAACGTGATCTTCAC
GAACGTCGCGCTCACGGAGGACGGCGACGTCTGGTGGGAAGGCCTCACCGACACGCCGCCCGCGCGGCTCACCGATTGGC
AGGGCAACGCATGGACGCCCGAGATCGGCCGCGAGACGGGCCGCAAGGCCGCGCATCCGAACTCGCGCTTCACGGCGCCC
GCGTCGCAGTGCCCGTCGATCGACGACGACTGGGAGAACCCGGGCGGCGTGCCGATCGACGCGTTCATCTTCGGCGGCCG
CCGCTCGACGACGGTGCCGCTCGTCACCGAGGCGCGCGACTGGATCGAAGGCGTGTACATGGCGGCGACGATGGGCTCGG
AGACGACGGCCGCGGCGGCGGGGCAGCAGGGCATCGTGCGGCGCGATCCGTTCGCGATGCTGCCGTTCTGCGGCTACAAC
ATGAGCGACTATTTCTCGCACTGGCTCGCGCTCGGCGAGAAGCTCGCGGCGGCGGGCGCGACGTTGCCGAAGATCTACTG
CGTGAACTGGTTCCGCAAGGACGCGGACGGCCGCTTCGCGTGGCCCGGCTTCGGCGAGAACATGCGCGTGCTGAAGTGGA
TGCTCGACCGGATCGACGGCCGCGGCGAGGGCGTCGAGCACGCGTTCGGCGTGACGCCGCGCTACGAGGATCTGCATTGG
GCGGGGCTCGCGTTCTCGCCCGCGCAGTATGCGCAGGTCACGTCGATGAATCCGGACGAATGGCGCGCCGAGCTCGCGCT
GCACGCGGAGCTGTTCGACAAGCTGAGCGCGCGGCTGCCGGATGCGCTCGCCGAAACGAAGGCGAGGATCGAGAAAAGGC
TCGGCGGCTGA

Upstream 100 bases:

>100_bases
ATCGCGTAGCGCCGCTACGCCCGCCGCGCGCGTCATCGCGCGGCGCACGCACGCAATTCTGGTTCTACAACACGGAGACA
CTCTCATGACCCGAAGCAAC

Downstream 100 bases:

>100_bases
GCAACGCGGCAAATCGCGAGCACGCCGCGCGGCGGCGACGCGGCCGGGACGGCGCGCGCCGGAGCCGCGCGCGAGATATG
CCGCTCGCGCCGCCGTGCGC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MVAATRTVPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQQEYDRLCDAMVEQRTMVRLNPAKRPNSFLALSDPSD
VARVEDRTFICSEYRDDAGPTNHWVAPAEMRATLNGLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVVNMRI
MTRMGRAVLDALGERGEYVPCVHSVGRPLAAGEQDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALRIAST
MGRDEGWLAEHMLILGVTSPEGRKYHIAAAFPSACGKTNFAMLIPPKGFEGWRVTTIGDDIAWLKPGRDGRLYAINPEAG
YFGVAPGTGEKTNPNALATLRENVIFTNVALTEDGDVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSRFTAP
ASQCPSIDDDWENPGGVPIDAFIFGGRRSTTVPLVTEARDWIEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLPFCGYN
MSDYFSHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDGRGEGVEHAFGVTPRYEDLHW
AGLAFSPAQYAQVTSMNPDEWRAELALHAELFDKLSARLPDALAETKARIEKRLGG

Sequences:

>Translated_616_residues
MVAATRTVPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQQEYDRLCDAMVEQRTMVRLNPAKRPNSFLALSDPSD
VARVEDRTFICSEYRDDAGPTNHWVAPAEMRATLNGLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVVNMRI
MTRMGRAVLDALGERGEYVPCVHSVGRPLAAGEQDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALRIAST
MGRDEGWLAEHMLILGVTSPEGRKYHIAAAFPSACGKTNFAMLIPPKGFEGWRVTTIGDDIAWLKPGRDGRLYAINPEAG
YFGVAPGTGEKTNPNALATLRENVIFTNVALTEDGDVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSRFTAP
ASQCPSIDDDWENPGGVPIDAFIFGGRRSTTVPLVTEARDWIEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLPFCGYN
MSDYFSHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDGRGEGVEHAFGVTPRYEDLHW
AGLAFSPAQYAQVTSMNPDEWRAELALHAELFDKLSARLPDALAETKARIEKRLGG
>Mature_616_residues
MVAATRTVPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQQEYDRLCDAMVEQRTMVRLNPAKRPNSFLALSDPSD
VARVEDRTFICSEYRDDAGPTNHWVAPAEMRATLNGLFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVVNMRI
MTRMGRAVLDALGERGEYVPCVHSVGRPLAAGEQDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALRIAST
MGRDEGWLAEHMLILGVTSPEGRKYHIAAAFPSACGKTNFAMLIPPKGFEGWRVTTIGDDIAWLKPGRDGRLYAINPEAG
YFGVAPGTGEKTNPNALATLRENVIFTNVALTEDGDVWWEGLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSRFTAP
ASQCPSIDDDWENPGGVPIDAFIFGGRRSTTVPLVTEARDWIEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLPFCGYN
MSDYFSHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDGRGEGVEHAFGVTPRYEDLHW
AGLAFSPAQYAQVTSMNPDEWRAELALHAELFDKLSARLPDALAETKARIEKRLGG

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=606, Percent_Identity=50.8250825082508, Blast_Score=592, Evalue=1e-169,
Organism=Homo sapiens, GI66346721, Length=610, Percent_Identity=50.327868852459, Blast_Score=573, Evalue=1e-163,
Organism=Homo sapiens, GI66346723, Length=387, Percent_Identity=52.9715762273902, Blast_Score=391, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71989645, Length=607, Percent_Identity=48.9291598023064, Blast_Score=563, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71989658, Length=607, Percent_Identity=48.9291598023064, Blast_Score=562, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI17555492, Length=617, Percent_Identity=49.2706645056726, Blast_Score=556, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71989653, Length=597, Percent_Identity=49.0787269681742, Blast_Score=554, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564411, Length=558, Percent_Identity=52.5089605734767, Blast_Score=547, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564407, Length=529, Percent_Identity=51.7958412098299, Blast_Score=509, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71989649, Length=482, Percent_Identity=53.1120331950208, Blast_Score=496, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI32564409, Length=375, Percent_Identity=49.0666666666667, Blast_Score=324, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17540956, Length=532, Percent_Identity=24.6240601503759, Blast_Score=171, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24655085, Length=597, Percent_Identity=49.5812395309883, Blast_Score=582, Evalue=1e-166,
Organism=Drosophila melanogaster, GI24655082, Length=607, Percent_Identity=48.1054365733114, Blast_Score=559, Evalue=1e-159,
Organism=Drosophila melanogaster, GI161077226, Length=576, Percent_Identity=48.7847222222222, Blast_Score=536, Evalue=1e-152,
Organism=Drosophila melanogaster, GI24655088, Length=509, Percent_Identity=50.8840864440079, Blast_Score=516, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 68003; Mature: 68003

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAATRTVPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQQEYDRLCDAMVEQRTM
CCCCCEEECCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCE
VRLNPAKRPNSFLALSDPSDVARVEDRTFICSEYRDDAGPTNHWVAPAEMRATLNGLFRG
EEECCCCCCCCEEEECCCCHHHHHCCCEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
AMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVVNMRIMTRMGRAVLDALGERGEYVP
HHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEH
CVHSVGRPLAAGEQDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALRIAST
HHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCHHCCHHHHHHHHHH
MGRDEGWLAEHMLILGVTSPEGRKYHIAAAFPSACGKTNFAMLIPPKGFEGWRVTTIGDD
CCCCCCHHEEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEEECCC
IAWLKPGRDGRLYAINPEAGYFGVAPGTGEKTNPNALATLRENVIFTNVALTEDGDVWWE
EEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHCCEEEEEEEEECCCCEEEC
GLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSRFTAPASQCPSIDDDWENPGGVPID
CCCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEE
AFIFGGRRSTTVPLVTEARDWIEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLPFCGYN
EEEECCCCCCEEEEEHHHHHHHHHEEEEEECCCCHHHHHCCCCCCEECCCCEECCCCCCC
MSDYFSHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDG
HHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCCCEECCCCCCCHHHHHHHHHHCCC
RGEGVEHAFGVTPRYEDLHWAGLAFSPAQYAQVTSMNPDEWRAELALHAELFDKLSARLP
CCCCCHHHCCCCCCCCCCEEECEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DALAETKARIEKRLGG
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVAATRTVPIDVPEYVKHRGLIDWVARIAELTEPDRVVWCDGSQQEYDRLCDAMVEQRTM
CCCCCEEECCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCE
VRLNPAKRPNSFLALSDPSDVARVEDRTFICSEYRDDAGPTNHWVAPAEMRATLNGLFRG
EEECCCCCCCCEEEECCCCHHHHHCCCEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
AMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVVVNMRIMTRMGRAVLDALGERGEYVP
HHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEH
CVHSVGRPLAAGEQDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALRIAST
HHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCHHCCHHHHHHHHHH
MGRDEGWLAEHMLILGVTSPEGRKYHIAAAFPSACGKTNFAMLIPPKGFEGWRVTTIGDD
CCCCCCHHEEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEEECCC
IAWLKPGRDGRLYAINPEAGYFGVAPGTGEKTNPNALATLRENVIFTNVALTEDGDVWWE
EEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHCCEEEEEEEEECCCCEEEC
GLTDTPPARLTDWQGNAWTPEIGRETGRKAAHPNSRFTAPASQCPSIDDDWENPGGVPID
CCCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEE
AFIFGGRRSTTVPLVTEARDWIEGVYMAATMGSETTAAAAGQQGIVRRDPFAMLPFCGYN
EEEECCCCCCEEEEEHHHHHHHHHEEEEEECCCCHHHHHCCCCCCEECCCCEECCCCCCC
MSDYFSHWLALGEKLAAAGATLPKIYCVNWFRKDADGRFAWPGFGENMRVLKWMLDRIDG
HHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCCCCEECCCCCCCHHHHHHHHHHCCC
RGEGVEHAFGVTPRYEDLHWAGLAFSPAQYAQVTSMNPDEWRAELALHAELFDKLSARLP
CCCCCHHHCCCCCCCCCCEEECEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DALAETKARIEKRLGG
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA