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The map label for this gene is fabI-2 [H]

Identifier: 23503018

GI number: 23503018

Start: 2094902

End: 2095720

Strand: Reverse

Name: fabI-2 [H]

Synonym: BR2171

Alternate gene names: 23503018

Gene position: 2095720-2094902 (Counterclockwise)

Preceding gene: 23503019

Following gene: 23503002

Centisome position: 99.43

GC content: 58.49

Gene sequence:

>819_bases
ATGGAAGGTTTGATGCAAGGAAAACGCGGCCTCATCATGGGGGTCGCGAACAATCACTCACTGGCCTGGGGCATTGCAAA
ACAGCTCGCCGCACAGGGCGCCGAACTGGCCTTCACCTATCAGGGTGATGCGCTGGGCAAGCGCGTGAAACCTCTTGCCG
AACAGGTCGGCTCGGATTTCGTGCTGCCTTGCGACGTGGAGGACATCGCCACCGTCGATGCCGTTTTCGAAGAAATCGAG
AAGAAGTGGGGCGGTCTCGATTTCCTCGTGCACGCTATCGGTTTTTCCGACAAGACGGAATTGAAGGGCCGCTATGCAGA
TGTCACCACGCGCGAGAATTTCAGCCGCACGATGGTCATCTCCGCCTATTCCTTCACAGAAGTGGCGCAGCGCGCTGAAA
AGCTCATGAAGGACGGCGGCTCGATCCTGACGCTGACCTATGGCGGATCGACCCGAACGATCCCCAATTACAACGTGATG
GGTGTTGCCAAGGCCGCTCTCGAGGCCATGGTGCGCTATCTTGCAGCCGATTACGGCCCGCAGGGCATCCGCGTCAACGC
GATTTCCGCAGGCCCCGTGCGCACGCTTGCTGGCGCCGGGATCGGTGATGCCCGCGCGATTTTCAGCTACCAGCGCCGCA
ACTCGCCGCTGCGCCGCACCGTCGATATCGATGATGTCGGCAAATCGGCAGTCTATCTTCTGTCCGATCTTTCAAGCGGT
GTGACCGGCGAAATCCATTTCGTTGATTCCGGCTATAACATCGTTTCGATGCCGACGCTGGAAGAGCTGAAAAGTTCGGA
CTCCGAACGCGGAGAATAA

Upstream 100 bases:

>100_bases
ATGTTCAGCTCAACACCGTGCTCTCCAATTCCTTCGGGTTTGGCGGCACCAACGCCACGCTGGTGTTTCAGCGGTATCAG
GGCTGACGGAGAGGGATAAT

Downstream 100 bases:

>100_bases
GCCGAAACATCTTATAAAAAGGCCGCTCTTCGGGGCGGCCTTTTTATTTACTTTTTGGCGTCGAATATCTTGAAGCCTTC
CGCCTCTTCGAGAAGCGTAA

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MEGLMQGKRGLIMGVANNHSLAWGIAKQLAAQGAELAFTYQGDALGKRVKPLAEQVGSDFVLPCDVEDIATVDAVFEEIE
KKWGGLDFLVHAIGFSDKTELKGRYADVTTRENFSRTMVISAYSFTEVAQRAEKLMKDGGSILTLTYGGSTRTIPNYNVM
GVAKAALEAMVRYLAADYGPQGIRVNAISAGPVRTLAGAGIGDARAIFSYQRRNSPLRRTVDIDDVGKSAVYLLSDLSSG
VTGEIHFVDSGYNIVSMPTLEELKSSDSERGE

Sequences:

>Translated_272_residues
MEGLMQGKRGLIMGVANNHSLAWGIAKQLAAQGAELAFTYQGDALGKRVKPLAEQVGSDFVLPCDVEDIATVDAVFEEIE
KKWGGLDFLVHAIGFSDKTELKGRYADVTTRENFSRTMVISAYSFTEVAQRAEKLMKDGGSILTLTYGGSTRTIPNYNVM
GVAKAALEAMVRYLAADYGPQGIRVNAISAGPVRTLAGAGIGDARAIFSYQRRNSPLRRTVDIDDVGKSAVYLLSDLSSG
VTGEIHFVDSGYNIVSMPTLEELKSSDSERGE
>Mature_272_residues
MEGLMQGKRGLIMGVANNHSLAWGIAKQLAAQGAELAFTYQGDALGKRVKPLAEQVGSDFVLPCDVEDIATVDAVFEEIE
KKWGGLDFLVHAIGFSDKTELKGRYADVTTRENFSRTMVISAYSFTEVAQRAEKLMKDGGSILTLTYGGSTRTIPNYNVM
GVAKAALEAMVRYLAADYGPQGIRVNAISAGPVRTLAGAGIGDARAIFSYQRRNSPLRRTVDIDDVGKSAVYLLSDLSSG
VTGEIHFVDSGYNIVSMPTLEELKSSDSERGE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI10190704, Length=224, Percent_Identity=29.4642857142857, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=50.965250965251, Blast_Score=271, Evalue=3e-74,
Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=29.6153846153846, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=27.3076923076923, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI2367365, Length=258, Percent_Identity=27.5193798449612, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=27.2, Blast_Score=65, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=187, Percent_Identity=26.2032085561497, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24644337, Length=206, Percent_Identity=29.126213592233, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24643142, Length=234, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29301; Mature: 29301

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGLMQGKRGLIMGVANNHSLAWGIAKQLAAQGAELAFTYQGDALGKRVKPLAEQVGSDF
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCC
VLPCDVEDIATVDAVFEEIEKKWGGLDFLVHAIGFSDKTELKGRYADVTTRENFSRTMVI
EEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHCCCEEECCCCCCCCCEEEEE
SAYSFTEVAQRAEKLMKDGGSILTLTYGGSTRTIPNYNVMGVAKAALEAMVRYLAADYGP
EEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
QGIRVNAISAGPVRTLAGAGIGDARAIFSYQRRNSPLRRTVDIDDVGKSAVYLLSDLSSG
CCEEEEEECCCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEECHHHCCHHHHHHHHHHCCC
VTGEIHFVDSGYNIVSMPTLEELKSSDSERGE
CCEEEEEEECCCCEEECCCHHHHHCCCCCCCC
>Mature Secondary Structure
MEGLMQGKRGLIMGVANNHSLAWGIAKQLAAQGAELAFTYQGDALGKRVKPLAEQVGSDF
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCC
VLPCDVEDIATVDAVFEEIEKKWGGLDFLVHAIGFSDKTELKGRYADVTTRENFSRTMVI
EEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHCCCEEECCCCCCCCCEEEEE
SAYSFTEVAQRAEKLMKDGGSILTLTYGGSTRTIPNYNVMGVAKAALEAMVRYLAADYGP
EEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
QGIRVNAISAGPVRTLAGAGIGDARAIFSYQRRNSPLRRTVDIDDVGKSAVYLLSDLSSG
CCEEEEEECCCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEECHHHCCHHHHHHHHHHCCC
VTGEIHFVDSGYNIVSMPTLEELKSSDSERGE
CCEEEEEEECCCCEEECCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]