Click here to switch to the map view.

The map label for this gene is pckA [H]

Identifier: 23502937

GI number: 23502937

Start: 2011046

End: 2012656

Strand: Reverse

Name: pckA [H]

Synonym: BR2089

Alternate gene names: 23502937

Gene position: 2012656-2011046 (Counterclockwise)

Preceding gene: 23502954

Following gene: 23502936

Centisome position: 95.49

GC content: 59.47

Gene sequence:

>1611_bases
ATGAAAGAGACCGGCATCCACAATAAGGCCGCTTCGATTTCCACTTCGGGACTGAAGGAACTCTCCGCAGTCTTCTACAA
TCTCGGGCCGGCACGGCTTTATGAAGAGACCATTCGCCGGGGCGAAGCGGAGCTTTCCGCGCAGGGCGCGCTCGTTGCCC
GTACCGGCCAGCACACGGGCCGTTCGCCGAAAGACAAGTTCGTCGTGCGCGACGCCAATACCGAAGATCATGTGTGGTGG
GACAACAACAAGCCCATGACGCCGGAAGCTTTTGAGCTTCTCTATGCCGATTTCATCGAACATGCGAAGGGCAGGGAGCT
TTTCGTTCAGGATCTCATCGGCGGCGCGGATGCCGACAACAAGATCAACGCCCGCGTCATCACCGAATATGCTTGGCATT
CGCTGTTCATCCGCAATCTGCTGATCCGTCCGTCGCAGGAAGCCCTTGCTTCCTACGTGCCGGAAATGACCATCATCGAC
CTGCCGTCCTTCAAGGCTGACCCGGAACGCTATGGCGTGCGCACCGAAACGGTGATCGCGGTCGATCTTACCCGCAAGAT
CGTGCTGATCGGCGGCACGTCCTATGCGGGCGAGATGAAGAAGTCCGTCTTCACCGCGCTCAACTATATCCTGCCTGCCA
AGGGCGTTATGCCGATGCACTGCTCGGCCAATGAAGGCCCGAACGGCGACACGGCGGTTTTCTTCGGTCTTTCGGGCACC
GGCAAGACCACGCTTTCCGCCGATCCGACGCGCACGCTGATCGGTGACGACGAACATGGCTGGGGCGAACATGGCGTCTT
CAATTTTGAAGGCGGCTGCTATGCCAAGACCATCCGTCTTTCCGCCGAAGCAGAACCGGAAATCTATGCCACGACGCAAC
GTTTCGGCACGGTTCTGGAAAATGTCGTGCTGGACGAGAATCGCCAGCCGGATTTCGACGATGGTTCGCTGACGGAAAAC
ACGCGCTGCGCCTATCCGCTGGATTTCATCCCCAACGCCTCGAAATCGGGCAAGGGCGGCCAGCCGAAGAATATCATCAT
GCTCACCGCCGATGCCTTCGGCGTGATGCCGCCCATCGCCAAGCTGACGCCGGCGCAGGCCATGTATCACTTCCTGTCCG
GCTATACCGCCAAGGTGGCAGGCACGGAAAAGGGCGTGACGGAACCGGAGGCAACCTTCTCCACCTGCTTCGGTGCGCCT
TTCATGCCGCGCCATCCTTCGGAATATGGCAACCTGTTGCGCAAGCTGATTGCCGAGCACAAGGTTGATTGCTGGCTGGT
CAATACCGGCTGGACGGGCGGCGCTTACGGCGTTGGCAAGCGGATGCCGATCAAGGCAACCCGCGCGCTTCTGGCAGCGG
CCCTCGACGGCTCGCTGAACAATGCTGAATTCCGCATCGATCCGAATTTCGGCTTCGCCGTCCCTGTCGAAGTGCCGGGT
GTGGAAAGCTCCATTCTCGATCCGCGCTCGACCTGGGCCGACAAAGTTGCCTATGACGCCCAGGCCAAGAAGCTGGTCGA
CATGTTCGTTTCCAACTTCGAGAAGTTTGAAAGCCACGTCGACCACGAGGTCAAGGATGCCGCACCGGCGATCCGCATGG
CTGCTGAATAG

Upstream 100 bases:

>100_bases
TTGAATTTTCTCAATTGATCTGTTTCTGACTGCCATCCCCCATGAAAGCCAAACCAGGGGGACGCATTTTTTAGTTAGTC
AAACAGACGGCGGAGACATC

Downstream 100 bases:

>100_bases
GTTTTAAGCATATTGAGGAGGGGGGAAGCCCGGGCCATCGCGCGGGGCTTCTTTTTTTGGCTGTTATCGCAAGAATTTCG
CAGGGCGCCAAATCAGTGTA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MKETGIHNKAASISTSGLKELSAVFYNLGPARLYEETIRRGEAELSAQGALVARTGQHTGRSPKDKFVVRDANTEDHVWW
DNNKPMTPEAFELLYADFIEHAKGRELFVQDLIGGADADNKINARVITEYAWHSLFIRNLLIRPSQEALASYVPEMTIID
LPSFKADPERYGVRTETVIAVDLTRKIVLIGGTSYAGEMKKSVFTALNYILPAKGVMPMHCSANEGPNGDTAVFFGLSGT
GKTTLSADPTRTLIGDDEHGWGEHGVFNFEGGCYAKTIRLSAEAEPEIYATTQRFGTVLENVVLDENRQPDFDDGSLTEN
TRCAYPLDFIPNASKSGKGGQPKNIIMLTADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSEYGNLLRKLIAEHKVDCWLVNTGWTGGAYGVGKRMPIKATRALLAAALDGSLNNAEFRIDPNFGFAVPVEVPG
VESSILDPRSTWADKVAYDAQAKKLVDMFVSNFEKFESHVDHEVKDAAPAIRMAAE

Sequences:

>Translated_536_residues
MKETGIHNKAASISTSGLKELSAVFYNLGPARLYEETIRRGEAELSAQGALVARTGQHTGRSPKDKFVVRDANTEDHVWW
DNNKPMTPEAFELLYADFIEHAKGRELFVQDLIGGADADNKINARVITEYAWHSLFIRNLLIRPSQEALASYVPEMTIID
LPSFKADPERYGVRTETVIAVDLTRKIVLIGGTSYAGEMKKSVFTALNYILPAKGVMPMHCSANEGPNGDTAVFFGLSGT
GKTTLSADPTRTLIGDDEHGWGEHGVFNFEGGCYAKTIRLSAEAEPEIYATTQRFGTVLENVVLDENRQPDFDDGSLTEN
TRCAYPLDFIPNASKSGKGGQPKNIIMLTADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSEYGNLLRKLIAEHKVDCWLVNTGWTGGAYGVGKRMPIKATRALLAAALDGSLNNAEFRIDPNFGFAVPVEVPG
VESSILDPRSTWADKVAYDAQAKKLVDMFVSNFEKFESHVDHEVKDAAPAIRMAAE
>Mature_536_residues
MKETGIHNKAASISTSGLKELSAVFYNLGPARLYEETIRRGEAELSAQGALVARTGQHTGRSPKDKFVVRDANTEDHVWW
DNNKPMTPEAFELLYADFIEHAKGRELFVQDLIGGADADNKINARVITEYAWHSLFIRNLLIRPSQEALASYVPEMTIID
LPSFKADPERYGVRTETVIAVDLTRKIVLIGGTSYAGEMKKSVFTALNYILPAKGVMPMHCSANEGPNGDTAVFFGLSGT
GKTTLSADPTRTLIGDDEHGWGEHGVFNFEGGCYAKTIRLSAEAEPEIYATTQRFGTVLENVVLDENRQPDFDDGSLTEN
TRCAYPLDFIPNASKSGKGGQPKNIIMLTADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSEYGNLLRKLIAEHKVDCWLVNTGWTGGAYGVGKRMPIKATRALLAAALDGSLNNAEFRIDPNFGFAVPVEVPG
VESSILDPRSTWADKVAYDAQAKKLVDMFVSNFEKFESHVDHEVKDAAPAIRMAAE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=520, Percent_Identity=48.8461538461538, Blast_Score=498, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6322950, Length=519, Percent_Identity=45.8574181117534, Blast_Score=475, Evalue=1e-135,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58585; Mature: 58585

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETGIHNKAASISTSGLKELSAVFYNLGPARLYEETIRRGEAELSAQGALVARTGQHTG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCEEEEECCCCCC
RSPKDKFVVRDANTEDHVWWDNNKPMTPEAFELLYADFIEHAKGRELFVQDLIGGADADN
CCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
KINARVITEYAWHSLFIRNLLIRPSQEALASYVPEMTIIDLPSFKADPERYGVRTETVIA
CCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCHHHHCCCEEEEEE
VDLTRKIVLIGGTSYAGEMKKSVFTALNYILPAKGVMPMHCSANEGPNGDTAVFFGLSGT
EEECEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEECCC
GKTTLSADPTRTLIGDDEHGWGEHGVFNFEGGCYAKTIRLSAEAEPEIYATTQRFGTVLE
CCEEECCCCCEEEECCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEHHHHHHHHHH
NVVLDENRQPDFDDGSLTENTRCAYPLDFIPNASKSGKGGQPKNIIMLTADAFGVMPPIA
HHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHH
KLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSEYGNLLRKLIAEH
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
KVDCWLVNTGWTGGAYGVGKRMPIKATRALLAAALDGSLNNAEFRIDPNFGFAVPVEVPG
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCC
VESSILDPRSTWADKVAYDAQAKKLVDMFVSNFEKFESHVDHEVKDAAPAIRMAAE
CCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCCC
>Mature Secondary Structure
MKETGIHNKAASISTSGLKELSAVFYNLGPARLYEETIRRGEAELSAQGALVARTGQHTG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCEEEEECCCCCC
RSPKDKFVVRDANTEDHVWWDNNKPMTPEAFELLYADFIEHAKGRELFVQDLIGGADADN
CCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
KINARVITEYAWHSLFIRNLLIRPSQEALASYVPEMTIIDLPSFKADPERYGVRTETVIA
CCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCHHHHCCCEEEEEE
VDLTRKIVLIGGTSYAGEMKKSVFTALNYILPAKGVMPMHCSANEGPNGDTAVFFGLSGT
EEECEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEECCC
GKTTLSADPTRTLIGDDEHGWGEHGVFNFEGGCYAKTIRLSAEAEPEIYATTQRFGTVLE
CCEEECCCCCEEEECCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEHHHHHHHHHH
NVVLDENRQPDFDDGSLTENTRCAYPLDFIPNASKSGKGGQPKNIIMLTADAFGVMPPIA
HHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHH
KLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSEYGNLLRKLIAEH
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
KVDCWLVNTGWTGGAYGVGKRMPIKATRALLAAALDGSLNNAEFRIDPNFGFAVPVEVPG
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCC
VESSILDPRSTWADKVAYDAQAKKLVDMFVSNFEKFESHVDHEVKDAAPAIRMAAE
CCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA