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The map label for this gene is dacF [H]

Identifier: 23502884

GI number: 23502884

Start: 1960763

End: 1961878

Strand: Reverse

Name: dacF [H]

Synonym: BR2036

Alternate gene names: 23502884

Gene position: 1961878-1960763 (Counterclockwise)

Preceding gene: 23502887

Following gene: 23502883

Centisome position: 93.08

GC content: 60.66

Gene sequence:

>1116_bases
ATGATGTCGAAGTCATTGAGAATCTGGTTTGCGGCAGTGGCCTTTTCCGGTATCGCGTTGTCGGGCGCGGCGGCCAATCC
GTCCATTGCCGTCGATGTGGCCACCGGCAAGGTTTATGAACAGCAGGATGCCTTCCAGCGCTGGTATCCCGCTTCGCTCA
CCAAGATGATGACGGCCTATGTCACATTCCGCGCGCTTCAGTCCGGCCAGATGACGCTGGAATCCCCGGTGCGCATGACG
GTCAACGCCTCCAAGGAACCGCCGAGCAAGATGGGGTACAAGCCCGGCTCGATCATGACGCTTGATACGGCGCTGAACAT
CATGCTGGTCAAGTCTGCCAATGATGTTTCGGTGGCCGTTGCCGAAGCCGTGGGCGGAACGGAAGCTGCCTTCGTGCAGC
GGATGAATGCCGAGGCGCGGCGTCTTGGAATGTTCGGCTCGCATTTCGCCAATCCGAATGGCCTGCATGATCCGAACAAT
TATACGACAGCGCGTGATCTTGCGGTTCTTGCCGTGCAGTTGCGCCGCGAATTTCCGCAATATGCGCATTATTTCGCGAC
AGAAGCGATCGATCCGGGTGCGGGCAAGAAAGTTGAGGCCAATTACAACATCCTGCTTGGCCGCTATGACGGTGCGGATG
GCATGAAGACGGGCTTCGTCTGCGCATCAGGCTTCAACCTTGCAGGTTCGGCCACCCGCAACGGCCGCACGGTGCTGGCA
ATCGTACTGGGTGCGGATCGCCAGGAAACGCGTGCGATCCAGGCCGCGCAAATGATGACCGATGCGTTTCGCGCGCGCAG
TTCCGGCGGGCCGACGCTTGCCACGCTGCGCCCGGCGCAGACAGCCAATCTCAATCAGGCCGTTGATATGCGCAAGGCCA
TCTGTAGCGAGCAGGCCATGGCCGACCGCTGGGACGGGCGCGAGGTAGAAGGCCGGCTGAAGATCAATTCACCCTATATT
CACGCCATGGACCATGATCCGATTGCCGTGCAGGTGAGGCTGGTTTCCGAACCGGGTTCGATCAACCGGCCCGGCCAGCC
CGGCATTTCGCGGGTTCCGGTGCCAATGCCGCGTCCGCAGCGCGCGCCAGGCGTAAAGACAACGGCCATCAACTGA

Upstream 100 bases:

>100_bases
AAGTGCTTATGAATCGTTATCGTGACGATTGCTACGGTTCGCTACGAGGCTGCTGCGATAACCCGCAAAAATTGCGGGCC
ATTGTGATTGGAACTGCGGA

Downstream 100 bases:

>100_bases
TATGCCTGATCCCATTCCCGTTTCCGTGCTGACCGGCTTTCTTGGCTCCGGCAAGACGACGCTGCTCAACCGGCTTTTGA
AAGATCCGGCGCTCAGCGAT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MMSKSLRIWFAAVAFSGIALSGAAANPSIAVDVATGKVYEQQDAFQRWYPASLTKMMTAYVTFRALQSGQMTLESPVRMT
VNASKEPPSKMGYKPGSIMTLDTALNIMLVKSANDVSVAVAEAVGGTEAAFVQRMNAEARRLGMFGSHFANPNGLHDPNN
YTTARDLAVLAVQLRREFPQYAHYFATEAIDPGAGKKVEANYNILLGRYDGADGMKTGFVCASGFNLAGSATRNGRTVLA
IVLGADRQETRAIQAAQMMTDAFRARSSGGPTLATLRPAQTANLNQAVDMRKAICSEQAMADRWDGREVEGRLKINSPYI
HAMDHDPIAVQVRLVSEPGSINRPGQPGISRVPVPMPRPQRAPGVKTTAIN

Sequences:

>Translated_371_residues
MMSKSLRIWFAAVAFSGIALSGAAANPSIAVDVATGKVYEQQDAFQRWYPASLTKMMTAYVTFRALQSGQMTLESPVRMT
VNASKEPPSKMGYKPGSIMTLDTALNIMLVKSANDVSVAVAEAVGGTEAAFVQRMNAEARRLGMFGSHFANPNGLHDPNN
YTTARDLAVLAVQLRREFPQYAHYFATEAIDPGAGKKVEANYNILLGRYDGADGMKTGFVCASGFNLAGSATRNGRTVLA
IVLGADRQETRAIQAAQMMTDAFRARSSGGPTLATLRPAQTANLNQAVDMRKAICSEQAMADRWDGREVEGRLKINSPYI
HAMDHDPIAVQVRLVSEPGSINRPGQPGISRVPVPMPRPQRAPGVKTTAIN
>Mature_371_residues
MMSKSLRIWFAAVAFSGIALSGAAANPSIAVDVATGKVYEQQDAFQRWYPASLTKMMTAYVTFRALQSGQMTLESPVRMT
VNASKEPPSKMGYKPGSIMTLDTALNIMLVKSANDVSVAVAEAVGGTEAAFVQRMNAEARRLGMFGSHFANPNGLHDPNN
YTTARDLAVLAVQLRREFPQYAHYFATEAIDPGAGKKVEANYNILLGRYDGADGMKTGFVCASGFNLAGSATRNGRTVLA
IVLGADRQETRAIQAAQMMTDAFRARSSGGPTLATLRPAQTANLNQAVDMRKAICSEQAMADRWDGREVEGRLKINSPYI
HAMDHDPIAVQVRLVSEPGSINRPGQPGISRVPVPMPRPQRAPGVKTTAIN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=243, Percent_Identity=32.5102880658436, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87082022, Length=241, Percent_Identity=31.1203319502075, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786851, Length=257, Percent_Identity=32.295719844358, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 39939; Mature: 39939

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSKSLRIWFAAVAFSGIALSGAAANPSIAVDVATGKVYEQQDAFQRWYPASLTKMMTAY
CCCCCHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHH
VTFRALQSGQMTLESPVRMTVNASKEPPSKMGYKPGSIMTLDTALNIMLVKSANDVSVAV
HHHHHHHCCCEEECCCEEEEEECCCCCCHHHCCCCCCEEEEECCEEEEEEECCCCCEEEE
AEAVGGTEAAFVQRMNAEARRLGMFGSHFANPNGLHDPNNYTTARDLAVLAVQLRREFPQ
EHHCCCCHHHHHHHHCHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YAHYFATEAIDPGAGKKVEANYNILLGRYDGADGMKTGFVCASGFNLAGSATRNGRTVLA
HHHHHHHHCCCCCCCCEEECCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCEEEE
IVLGADRQETRAIQAAQMMTDAFRARSSGGPTLATLRPAQTANLNQAVDMRKAICSEQAM
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
ADRWDGREVEGRLKINSPYIHAMDHDPIAVQVRLVSEPGSINRPGQPGISRVPVPMPRPQ
HHCCCCCEEEEEEEECCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
RAPGVKTTAIN
CCCCCEEECCC
>Mature Secondary Structure
MMSKSLRIWFAAVAFSGIALSGAAANPSIAVDVATGKVYEQQDAFQRWYPASLTKMMTAY
CCCCCHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHH
VTFRALQSGQMTLESPVRMTVNASKEPPSKMGYKPGSIMTLDTALNIMLVKSANDVSVAV
HHHHHHHCCCEEECCCEEEEEECCCCCCHHHCCCCCCEEEEECCEEEEEEECCCCCEEEE
AEAVGGTEAAFVQRMNAEARRLGMFGSHFANPNGLHDPNNYTTARDLAVLAVQLRREFPQ
EHHCCCCHHHHHHHHCHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YAHYFATEAIDPGAGKKVEANYNILLGRYDGADGMKTGFVCASGFNLAGSATRNGRTVLA
HHHHHHHHCCCCCCCCEEECCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCEEEE
IVLGADRQETRAIQAAQMMTDAFRARSSGGPTLATLRPAQTANLNQAVDMRKAICSEQAM
EEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
ADRWDGREVEGRLKINSPYIHAMDHDPIAVQVRLVSEPGSINRPGQPGISRVPVPMPRPQ
HHCCCCCEEEEEEEECCCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
RAPGVKTTAIN
CCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]