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The map label for this gene is potI [H]

Identifier: 23502472

GI number: 23502472

Start: 1555726

End: 1556547

Strand: Reverse

Name: potI [H]

Synonym: BR1609

Alternate gene names: 23502472

Gene position: 1556547-1555726 (Counterclockwise)

Preceding gene: 23502473

Following gene: 23502470

Centisome position: 73.85

GC content: 59.0

Gene sequence:

>822_bases
ATGCAGAACAACTGGTCCCGCTTCAATATCGTATCGGTGGCGCTGGGTTTTGCCTTTCTCTACCTGCCCATCGTGCTTTT
GGTGATTTATTCGTTCAATGAATCCCGTCTGGTCACGGTCTGGGCCGGGTTCTCGACCAAATGGTATGGCGAATTGTTCC
GCAATCAGGCCCTGATGGATGCGGCCTGGGTGACGATCCGCGTCGGCCTTCTGTCCGCGACGATCGCCACGGTTCTGGGC
ACACTCGCAGCCCTTGCGCTCACGCGTTATACCCGCTTTCGCGGGCGCATCCTGTTTTCAGGCATGGTCTATGCGCCGCT
GGTCATGCCCGATGTCATCACCGGCCTGTCGCTGCTTCTGCTCTTCGTGGCGATGAATTTCGACCGCGGTTTCTGGACAG
TGACACTTGCCCATATTACCTTTTCCATGTGTTTCGTGGCGGTCGTGGTGCAATCGCGCCTCGTCAGCTTTGACCGGTCA
CTGGAAGAAGCGGCGATGGACCTCGGCGCGCCGCCGGTCACGACCTTCATGAAGGTGACGCTGCCGGTGATCCTGCCTGC
CGTGGTTTCCGGCTGGATGCTTGCTTTCACCCTGTCGCTCGACGATCTGGTGATTGCGAGTTTCACCTCCGGTCCGGGGG
CAACCACGCTGCCGATGAAAATCTACAGCCAGGTCCGCCTTGGTGTGACGCCGGAAATCAACGCGGTCTGCACCATTCTC
ATCGCGCTGGTGACAACGGGCGTCATTATCGCCTCCGTCATCAACAAGCGCCGTGAAGTGCAGCGCCGCCGCGACGAACA
GGCAGCATTCCGTATGGGATAA

Upstream 100 bases:

>100_bases
GTCTCCTCGGCGGTGGCGGTGGTGCTGCTCGTCCTGCTGATTGTGTCGATCGTCATTTTCCAGCAGGTGCAGGCGCGGGC
ACAGGAAAAGGGGAAATAGG

Downstream 100 bases:

>100_bases
AGGGACTTTACTGCCCCGTTCCCATCGAGGATGGTGAGATGAAAGGCCGATCCCACGAGCCGCCGACAAAGAAATGAAGC
GGCGGCTTGGCGGGCGGTGT

Product: spermidine/putrescine ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MQNNWSRFNIVSVALGFAFLYLPIVLLVIYSFNESRLVTVWAGFSTKWYGELFRNQALMDAAWVTIRVGLLSATIATVLG
TLAALALTRYTRFRGRILFSGMVYAPLVMPDVITGLSLLLLFVAMNFDRGFWTVTLAHITFSMCFVAVVVQSRLVSFDRS
LEEAAMDLGAPPVTTFMKVTLPVILPAVVSGWMLAFTLSLDDLVIASFTSGPGATTLPMKIYSQVRLGVTPEINAVCTIL
IALVTTGVIIASVINKRREVQRRRDEQAAFRMG

Sequences:

>Translated_273_residues
MQNNWSRFNIVSVALGFAFLYLPIVLLVIYSFNESRLVTVWAGFSTKWYGELFRNQALMDAAWVTIRVGLLSATIATVLG
TLAALALTRYTRFRGRILFSGMVYAPLVMPDVITGLSLLLLFVAMNFDRGFWTVTLAHITFSMCFVAVVVQSRLVSFDRS
LEEAAMDLGAPPVTTFMKVTLPVILPAVVSGWMLAFTLSLDDLVIASFTSGPGATTLPMKIYSQVRLGVTPEINAVCTIL
IALVTTGVIIASVINKRREVQRRRDEQAAFRMG
>Mature_273_residues
MQNNWSRFNIVSVALGFAFLYLPIVLLVIYSFNESRLVTVWAGFSTKWYGELFRNQALMDAAWVTIRVGLLSATIATVLG
TLAALALTRYTRFRGRILFSGMVYAPLVMPDVITGLSLLLLFVAMNFDRGFWTVTLAHITFSMCFVAVVVQSRLVSFDRS
LEEAAMDLGAPPVTTFMKVTLPVILPAVVSGWMLAFTLSLDDLVIASFTSGPGATTLPMKIYSQVRLGVTPEINAVCTIL
IALVTTGVIIASVINKRREVQRRRDEQAAFRMG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=272, Percent_Identity=57.7205882352941, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=45.1882845188285, Blast_Score=217, Evalue=6e-58,
Organism=Escherichia coli, GI1787714, Length=239, Percent_Identity=35.1464435146443, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788764, Length=176, Percent_Identity=34.0909090909091, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30133; Mature: 30133

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]