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The map label for this gene is yxeI [H]

Identifier: 23502337

GI number: 23502337

Start: 1424051

End: 1425052

Strand: Reverse

Name: yxeI [H]

Synonym: BR1468

Alternate gene names: 23502337

Gene position: 1425052-1424051 (Counterclockwise)

Preceding gene: 23502340

Following gene: 23502336

Centisome position: 67.61

GC content: 53.79

Gene sequence:

>1002_bases
ATGGCTTGCACGAGCTTCGTTCTGCCGACGAGCGACGGTGGTATGGTCTATGGTCGCACGATGGAATTCGGGTTCAATCT
CAAATCCGACATGATTGCCATTCCGCGCAATTACACCATCACGGCAAGCGGGCCGGACGGTGCTGCGGGCAAGAAATGGA
AGGGCAAATATGCCACGATCGGCATGAATGCTTTTGGTATCGTCGCTCTCACCGACGGTATGAACGAGAAGGGGCTTGCA
GGCGGGCTTCTCTATTTCCCGGAATATGCCAAGTATCAGGACCCATCCACGGCGAAGCCGGAAGACAGCCTCGCTCCGTG
GGATTTCCTGACCTGGGCGCTGACCAATTTTTCGACAGTGGCCGAAGTCAAGGATGCTTTGAGCACCATTTCCATCGTCG
ATGTGAAACAAAAGGACCTGGGATTTACCCCGCCCGCTCACTACACGCTGCATGATGCGACCGGCGCATCCATCGTGATC
GAACCGATCGACGGCAAGCTCAAGGTTTACGACAACAAGCTCGGTGTCATGACCAATTCGCCGTCTTTCGACTGGCACAT
GACCAATCTGCGCAACTATGTCTATCTCTCGCGTGAAAATCCGAAGCCGTTGCAGATCCTTGGCGAGACGATCCAGTCAT
TCGGGCAAGGCGCCGGTATGCATGGTATTCCGGGCGACACCACGCCGCCATCGCGTTTCGTGCGTGCAAGCGCCTACGTC
CTTTCCGCCAAGAAGGTGCCGAGCGGCCTTGAAAGCGTGCGGCTGGCCGAGCATATTGCCAATAACTTCGACATTCCAAA
GGGATGGAGCGAAGAGCAGAATATGTTTGAATATACCCAGTGGACCGCCTTTGCGGACATGAAGAACGATGTCTATTACA
TCAAGACCTATGACGATCAGGTTCTGCGCAGCTTCAGCTTCAAGGATTTTGATGTCGATAGCAAAGATATTCTGACGATC
AAGTTCGAGCCAAAACTGGACGCGCCGTCACTGAAAAAGTAA

Upstream 100 bases:

>100_bases
GGAAACGAAAAGCTCTCTCTGGAAATCATCGCGCCGCGTGCTTGCACATGGGGCTGCAACTGTTCTGGTCGCGGCGGGCC
TTATCGTTCCCCAGGCGGCT

Downstream 100 bases:

>100_bases
GGCGACCATGAAAAAGGCGGCTTCGGCCGCCTTTTGACTTATCAGGGCATAATCCGGTCGGGATGACGAAGGCCGACAGG
CTTATGCCTCTACAAAAGGG

Product: choloylglycine hydrolase family protein

Products: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MACTSFVLPTSDGGMVYGRTMEFGFNLKSDMIAIPRNYTITASGPDGAAGKKWKGKYATIGMNAFGIVALTDGMNEKGLA
GGLLYFPEYAKYQDPSTAKPEDSLAPWDFLTWALTNFSTVAEVKDALSTISIVDVKQKDLGFTPPAHYTLHDATGASIVI
EPIDGKLKVYDNKLGVMTNSPSFDWHMTNLRNYVYLSRENPKPLQILGETIQSFGQGAGMHGIPGDTTPPSRFVRASAYV
LSAKKVPSGLESVRLAEHIANNFDIPKGWSEEQNMFEYTQWTAFADMKNDVYYIKTYDDQVLRSFSFKDFDVDSKDILTI
KFEPKLDAPSLKK

Sequences:

>Translated_333_residues
MACTSFVLPTSDGGMVYGRTMEFGFNLKSDMIAIPRNYTITASGPDGAAGKKWKGKYATIGMNAFGIVALTDGMNEKGLA
GGLLYFPEYAKYQDPSTAKPEDSLAPWDFLTWALTNFSTVAEVKDALSTISIVDVKQKDLGFTPPAHYTLHDATGASIVI
EPIDGKLKVYDNKLGVMTNSPSFDWHMTNLRNYVYLSRENPKPLQILGETIQSFGQGAGMHGIPGDTTPPSRFVRASAYV
LSAKKVPSGLESVRLAEHIANNFDIPKGWSEEQNMFEYTQWTAFADMKNDVYYIKTYDDQVLRSFSFKDFDVDSKDILTI
KFEPKLDAPSLKK
>Mature_332_residues
ACTSFVLPTSDGGMVYGRTMEFGFNLKSDMIAIPRNYTITASGPDGAAGKKWKGKYATIGMNAFGIVALTDGMNEKGLAG
GLLYFPEYAKYQDPSTAKPEDSLAPWDFLTWALTNFSTVAEVKDALSTISIVDVKQKDLGFTPPAHYTLHDATGASIVIE
PIDGKLKVYDNKLGVMTNSPSFDWHMTNLRNYVYLSRENPKPLQILGETIQSFGQGAGMHGIPGDTTPPSRFVRASAYVL
SAKKVPSGLESVRLAEHIANNFDIPKGWSEEQNMFEYTQWTAFADMKNDVYYIKTYDDQVLRSFSFKDFDVDSKDILTIK
FEPKLDAPSLKK

Specific function: Unknown

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: 3.5.1.24

Molecular weight: Translated: 36865; Mature: 36734

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACTSFVLPTSDGGMVYGRTMEFGFNLKSDMIAIPRNYTITASGPDGAAGKKWKGKYATI
CCCCEEEEECCCCCEEEEEEEEECCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCEEEE
GMNAFGIVALTDGMNEKGLAGGLLYFPEYAKYQDPSTAKPEDSLAPWDFLTWALTNFSTV
CCCCEEEEEEECCCCCCCCCCCEEECCCHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHH
AEVKDALSTISIVDVKQKDLGFTPPAHYTLHDATGASIVIEPIDGKLKVYDNKLGVMTNS
HHHHHHHHHEEEEEECHHCCCCCCCCCEEEECCCCCEEEEEECCCEEEEEECEEEEEECC
PSFDWHMTNLRNYVYLSRENPKPLQILGETIQSFGQGAGMHGIPGDTTPPSRFVRASAYV
CCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHEEE
LSAKKVPSGLESVRLAEHIANNFDIPKGWSEEQNMFEYTQWTAFADMKNDVYYIKTYDDQ
EEHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEEEECCCCEEEEEECCHH
VLRSFSFKDFDVDSKDILTIKFEPKLDAPSLKK
HHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ACTSFVLPTSDGGMVYGRTMEFGFNLKSDMIAIPRNYTITASGPDGAAGKKWKGKYATI
CCCEEEEECCCCCEEEEEEEEECCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCEEEE
GMNAFGIVALTDGMNEKGLAGGLLYFPEYAKYQDPSTAKPEDSLAPWDFLTWALTNFSTV
CCCCEEEEEEECCCCCCCCCCCEEECCCHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHH
AEVKDALSTISIVDVKQKDLGFTPPAHYTLHDATGASIVIEPIDGKLKVYDNKLGVMTNS
HHHHHHHHHEEEEEECHHCCCCCCCCCEEEECCCCCEEEEEECCCEEEEEECEEEEEECC
PSFDWHMTNLRNYVYLSRENPKPLQILGETIQSFGQGAGMHGIPGDTTPPSRFVRASAYV
CCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHEEE
LSAKKVPSGLESVRLAEHIANNFDIPKGWSEEQNMFEYTQWTAFADMKNDVYYIKTYDDQ
EEHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEEEECCCCEEEEEECCHH
VLRSFSFKDFDVDSKDILTIKFEPKLDAPSLKK
HHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine; H2O

Specific reaction: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]