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The map label for this gene is murE [H]

Identifier: 23502307

GI number: 23502307

Start: 1394517

End: 1395983

Strand: Reverse

Name: murE [H]

Synonym: BR1436

Alternate gene names: 23502307

Gene position: 1395983-1394517 (Counterclockwise)

Preceding gene: 23502308

Following gene: 23502306

Centisome position: 66.23

GC content: 59.85

Gene sequence:

>1467_bases
ATGAAACTGAAGGAAATCGCTCTCTTTAATGAGCTGGCCTCCGGCGAGGCCGGTGAAGTGGAAATTACCGGCATCACTTC
GGATTCCCGCGCTGTTCAACGCGGTTTTCTTTTTGCGGCGCTGAAAGGCGTGAAAGCGGACGGTGCGGTTTTCGCCGCCG
ATGCCGTAAAGCGCGGTGCTGTCGCCATCATCGCGGGCAAGGACACGGCCATCGCTGATGCGGGTGTGCCGGTGCTTCAT
GTGGATGATCCGCGCCATGTTCTCGCCATTGCCGCCGCTCAATTTTATGGCAAGCAGCCGGAAGTCATGGTTGCCGTGAC
CGGCACCAGCGGCAAGACTTCCGTTGCCTCCTTCACGCGCCAGATCTGGGCCTATGCGGGGTTTCCCGCAGCCAATATCG
GCACGACCGGCGTGTTTTCGCCGACGCGCAGCGACTATAATTCCTTGACGACGCCCGACCCGGTGGAACTGCACCGCGTT
CTGGCCGAACTTACCAGCGAGGGCGTGACCCATGCGGCGATGGAAGCCTCGTCGCACGGTCTGGACCAGCGTCGTCTCGA
CGGGGTGAGGCTTGCTGCGGGCGCCTTCACCAATCTTGGCCGCGATCACATGGATTATCACGCGACCATTGATGAATATC
TGGGCGCGAAAATGCGCCTGTTCAACGCGCTTCTGCCGAAGGGTGCGCCAGCCATCATCTTTGCCGACGATCAATTTTCG
GCGCAGGCCATCGAAGCGGCGACGCTTGCTGGATGCGACGTGAAGACGGTCGGTCGAAAAGGCAATTTCATTGCCCTCAA
GCGCGTGGAACACGAGCGTTTCCGCCAGCATGTGGAAGTGCGGATCGGCGAGGAAATCTTTGAAATCGAATTGCCGCTGG
CGGGAGATTTTCAGGTCGCCAATGCGCTGGTTGCAGCGGGGCTTGCCATGGTGACGGGCGTTCCGGCTGCGGCTGCCATG
CGTGCATTGGCACTTCTCAAAGGCGCACCGGGACGGCTTGATCTTGTCGGTGCAACGGAAGATGGTGCGCCAGCCTATGT
TGATTATGCCCATAAGCCGGAAGCGTTGGAAAATGTGCTGACCTCCGTGCGTCCGTTTACGACGGGCCGTGTGATTGTGG
TCTTTGGCTGCGGCGGCGACCGCGACAAGGGCAAGCGCCCGATCATGGGTGAGATTGCCTCGCGTCTTGCCGATGTCGTG
ATTGTCACCGACGACAATCCCCGTTCGGAAGTGCCAGCACAGATACGTTCCGAAATCATGGCAGCGGCAACGGGAGCGAC
GGAAATTGGCGACCGACGCGAGGCGATCTTCACCGCCGTTTCCATGATGCAGCCCGGCGATACGCTGGTGGTTGCGGGCA
AGGGACATGAGGAAGGGCAGATCGTCGGCAATATTACGCTGCCGTTTTCAGATCATGCAGAAGTGGCGGCGGCACTTGCC
GCCCGTCTTGAGGAGCATCCAAAATGA

Upstream 100 bases:

>100_bases
GGAAGTGCCGCCAGCAATGGTTTCAAACGAATACGATTGATTCGCATAAGGCTGTCTCTGTATCGACGTGCACGCAACTG
AACCTGGATTTGGTATGACC

Downstream 100 bases:

>100_bases
GCAATCGGGAATTGAGCGATTGGCTCTGGACATCCGACGCCATGGTGGAAGCCATGGAAGGCAGGCCGTTCGGCAATCTG
CCGGCGGGAGTAACCGGCAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKLKEIALFNELASGEAGEVEITGITSDSRAVQRGFLFAALKGVKADGAVFAADAVKRGAVAIIAGKDTAIADAGVPVLH
VDDPRHVLAIAAAQFYGKQPEVMVAVTGTSGKTSVASFTRQIWAYAGFPAANIGTTGVFSPTRSDYNSLTTPDPVELHRV
LAELTSEGVTHAAMEASSHGLDQRRLDGVRLAAGAFTNLGRDHMDYHATIDEYLGAKMRLFNALLPKGAPAIIFADDQFS
AQAIEAATLAGCDVKTVGRKGNFIALKRVEHERFRQHVEVRIGEEIFEIELPLAGDFQVANALVAAGLAMVTGVPAAAAM
RALALLKGAPGRLDLVGATEDGAPAYVDYAHKPEALENVLTSVRPFTTGRVIVVFGCGGDRDKGKRPIMGEIASRLADVV
IVTDDNPRSEVPAQIRSEIMAAATGATEIGDRREAIFTAVSMMQPGDTLVVAGKGHEEGQIVGNITLPFSDHAEVAAALA
ARLEEHPK

Sequences:

>Translated_488_residues
MKLKEIALFNELASGEAGEVEITGITSDSRAVQRGFLFAALKGVKADGAVFAADAVKRGAVAIIAGKDTAIADAGVPVLH
VDDPRHVLAIAAAQFYGKQPEVMVAVTGTSGKTSVASFTRQIWAYAGFPAANIGTTGVFSPTRSDYNSLTTPDPVELHRV
LAELTSEGVTHAAMEASSHGLDQRRLDGVRLAAGAFTNLGRDHMDYHATIDEYLGAKMRLFNALLPKGAPAIIFADDQFS
AQAIEAATLAGCDVKTVGRKGNFIALKRVEHERFRQHVEVRIGEEIFEIELPLAGDFQVANALVAAGLAMVTGVPAAAAM
RALALLKGAPGRLDLVGATEDGAPAYVDYAHKPEALENVLTSVRPFTTGRVIVVFGCGGDRDKGKRPIMGEIASRLADVV
IVTDDNPRSEVPAQIRSEIMAAATGATEIGDRREAIFTAVSMMQPGDTLVVAGKGHEEGQIVGNITLPFSDHAEVAAALA
ARLEEHPK
>Mature_488_residues
MKLKEIALFNELASGEAGEVEITGITSDSRAVQRGFLFAALKGVKADGAVFAADAVKRGAVAIIAGKDTAIADAGVPVLH
VDDPRHVLAIAAAQFYGKQPEVMVAVTGTSGKTSVASFTRQIWAYAGFPAANIGTTGVFSPTRSDYNSLTTPDPVELHRV
LAELTSEGVTHAAMEASSHGLDQRRLDGVRLAAGAFTNLGRDHMDYHATIDEYLGAKMRLFNALLPKGAPAIIFADDQFS
AQAIEAATLAGCDVKTVGRKGNFIALKRVEHERFRQHVEVRIGEEIFEIELPLAGDFQVANALVAAGLAMVTGVPAAAAM
RALALLKGAPGRLDLVGATEDGAPAYVDYAHKPEALENVLTSVRPFTTGRVIVVFGCGGDRDKGKRPIMGEIASRLADVV
IVTDDNPRSEVPAQIRSEIMAAATGATEIGDRREAIFTAVSMMQPGDTLVVAGKGHEEGQIVGNITLPFSDHAEVAAALA
ARLEEHPK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=471, Percent_Identity=37.5796178343949, Blast_Score=234, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51376; Mature: 51376

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKEIALFNELASGEAGEVEITGITSDSRAVQRGFLFAALKGVKADGAVFAADAVKRGA
CCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCCC
VAIIAGKDTAIADAGVPVLHVDDPRHVLAIAAAQFYGKQPEVMVAVTGTSGKTSVASFTR
EEEEECCCCEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH
QIWAYAGFPAANIGTTGVFSPTRSDYNSLTTPDPVELHRVLAELTSEGVTHAAMEASSHG
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCC
LDQRRLDGVRLAAGAFTNLGRDHMDYHATIDEYLGAKMRLFNALLPKGAPAIIFADDQFS
CCHHHCCCEEEEHHHHHHCCCCHHHHHCCHHHHHCHHHHHHHHHCCCCCCEEEEECCCCC
AQAIEAATLAGCDVKTVGRKGNFIALKRVEHERFRQHVEVRIGEEIFEIELPLAGDFQVA
HHHHHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
NALVAAGLAMVTGVPAAAAMRALALLKGAPGRLDLVGATEDGAPAYVDYAHKPEALENVL
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCHHHHHHHH
TSVRPFTTGRVIVVFGCGGDRDKGKRPIMGEIASRLADVVIVTDDNPRSEVPAQIRSEIM
HHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHH
AAATGATEIGDRREAIFTAVSMMQPGDTLVVAGKGHEEGQIVGNITLPFSDHAEVAAALA
HHHCCCHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHH
ARLEEHPK
HHHHHCCC
>Mature Secondary Structure
MKLKEIALFNELASGEAGEVEITGITSDSRAVQRGFLFAALKGVKADGAVFAADAVKRGA
CCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCCC
VAIIAGKDTAIADAGVPVLHVDDPRHVLAIAAAQFYGKQPEVMVAVTGTSGKTSVASFTR
EEEEECCCCEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH
QIWAYAGFPAANIGTTGVFSPTRSDYNSLTTPDPVELHRVLAELTSEGVTHAAMEASSHG
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCC
LDQRRLDGVRLAAGAFTNLGRDHMDYHATIDEYLGAKMRLFNALLPKGAPAIIFADDQFS
CCHHHCCCEEEEHHHHHHCCCCHHHHHCCHHHHHCHHHHHHHHHCCCCCCEEEEECCCCC
AQAIEAATLAGCDVKTVGRKGNFIALKRVEHERFRQHVEVRIGEEIFEIELPLAGDFQVA
HHHHHHHHHCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHH
NALVAAGLAMVTGVPAAAAMRALALLKGAPGRLDLVGATEDGAPAYVDYAHKPEALENVL
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCHHHHHHHH
TSVRPFTTGRVIVVFGCGGDRDKGKRPIMGEIASRLADVVIVTDDNPRSEVPAQIRSEIM
HHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHH
AAATGATEIGDRREAIFTAVSMMQPGDTLVVAGKGHEEGQIVGNITLPFSDHAEVAAALA
HHHCCCHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHH
ARLEEHPK
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]