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The map label for this gene is tuf [H]

Identifier: 23502112

GI number: 23502112

Start: 1200798

End: 1201973

Strand: Reverse

Name: tuf [H]

Synonym: BR1235

Alternate gene names: 23502112

Gene position: 1201973-1200798 (Counterclockwise)

Preceding gene: 23502113

Following gene: 23502111

Centisome position: 57.03

GC content: 58.93

Gene sequence:

>1176_bases
ATGGCAAAGAGTAAGTTTGAACGTACGAAGCCCCACGTTAACATCGGCACGATTGGTCACGTTGACCATGGCAAGACGTC
GCTGACGGCGGCGATCACGAAGTTTTTCGGCGAATTCAAGGCGTATGACCAGATCGACGCCGCGCCTGAAGAACGCGCCC
GCGGCATCACGATCTCGACGGCCCACGTCGAGTACGAGACCGCAAACCGTCACTATGCACACGTCGATTGCCCCGGCCAC
GCCGACTATGTGAAGAACATGATCACCGGCGCTGCCCAGATGGATGGCGCGATCCTGGTGGTTTCGGCTGCTGACGGCCC
GATGCCGCAGACCCGCGAGCACATCCTGCTTGCCCGTCAGGTTGGCGTTCCGGCGATCGTCGTGTTCCTCAACAAGTGCG
ACCAGGTTGACGATGCAGAACTGCTCGAACTGGTTGAACTGGAAGTGCGCGAACTTCTGTCGAAGTACGAATTCCCCGGC
GACGAAATCCCGATCATCAAGGGCTCGGCTCTTGCTGCTCTGGAAGATTCTTCCAAGGAACTGGGCGAAGATGCCATCCG
CAACCTGATGGACGCGGTTGACAGCTACATTCCGACCCCGGAACGCCCGATCGACCAGCCGTTCCTGATGCCGATCGAAG
ACGTGTTCTCGATCTCCGGCCGTGGTACGGTTGTGACGGGTCGCGTTGAGCGCGGTATCGTTAAGGTCGGTGAAGAAGTT
GAAATCGTCGGCATCAAGGCGACGACGAAGACCACGGTTACCGGCGTTGAAATGTTCCGCAAGCTGCTCGACCAGGGCCA
GGCTGGCGACAACATTGGCGCGCTGATCCGCGGCGTTGGCCGTGAAGACGTTGAACGCGGCCAGGTTCTCTGCAAGCCGG
GTTCTGTGAAGCCGCACACCAAGTTTAAGGCAGAAGCCTATATTCTGACCAAGGACGAAGGTGGCCGTCATACGCCGTTC
TTCACCAACTACCGTCCGCAGTTCTACTTCCGTACGACGGACGTGACGGGTGTTGTGACGCTTCCGGCTGGCACGGAAAT
GGTCATGCCTGGCGATAACGTCGCCATGGACGTTACCCTGATCGTGCCGATCGCCATGGAAGAGAAGCTTCGCTTCGCTA
TCCGTGAAGGCGGCCGCACCGTCGGTGCCGGCATCGTCTCGTCGATCATCGAGTAA

Upstream 100 bases:

>100_bases
GGCGGTCGCACAGGAAATCCAGAAGAAGTTTGCGTAACCGCTCAGGTTACGCTCCCAATCAAAAGAATGCCTGAGCAGGC
TAAATTTACGGAGAGCTCAA

Downstream 100 bases:

>100_bases
GAAGATTGTGCAGGGGCGCTCGTGTTGAGCGAGCGTCCCGCGTAACAAGGAACAGATGAAATGAACGGTCAAAACATCCG
CATTCGCCTGAAGGCGTTTG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 2 [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKSKFERTKPHVNIGTIGHVDHGKTSLTAAITKFFGEFKAYDQIDAAPEERARGITISTAHVEYETANRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDQVDDAELLELVELEVRELLSKYEFPG
DEIPIIKGSALAALEDSSKELGEDAIRNLMDAVDSYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
EIVGIKATTKTTVTGVEMFRKLLDQGQAGDNIGALIRGVGREDVERGQVLCKPGSVKPHTKFKAEAYILTKDEGGRHTPF
FTNYRPQFYFRTTDVTGVVTLPAGTEMVMPGDNVAMDVTLIVPIAMEEKLRFAIREGGRTVGAGIVSSIIE

Sequences:

>Translated_391_residues
MAKSKFERTKPHVNIGTIGHVDHGKTSLTAAITKFFGEFKAYDQIDAAPEERARGITISTAHVEYETANRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDQVDDAELLELVELEVRELLSKYEFPG
DEIPIIKGSALAALEDSSKELGEDAIRNLMDAVDSYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
EIVGIKATTKTTVTGVEMFRKLLDQGQAGDNIGALIRGVGREDVERGQVLCKPGSVKPHTKFKAEAYILTKDEGGRHTPF
FTNYRPQFYFRTTDVTGVVTLPAGTEMVMPGDNVAMDVTLIVPIAMEEKLRFAIREGGRTVGAGIVSSIIE
>Mature_390_residues
AKSKFERTKPHVNIGTIGHVDHGKTSLTAAITKFFGEFKAYDQIDAAPEERARGITISTAHVEYETANRHYAHVDCPGHA
DYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDQVDDAELLELVELEVRELLSKYEFPGD
EIPIIKGSALAALEDSSKELGEDAIRNLMDAVDSYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEVE
IVGIKATTKTTVTGVEMFRKLLDQGQAGDNIGALIRGVGREDVERGQVLCKPGSVKPHTKFKAEAYILTKDEGGRHTPFF
TNYRPQFYFRTTDVTGVVTLPAGTEMVMPGDNVAMDVTLIVPIAMEEKLRFAIREGGRTVGAGIVSSIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.3604060913706, Blast_Score=490, Evalue=1e-138,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.6380090497738, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=29.4663573085847, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=29.4663573085847, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4503475, Length=442, Percent_Identity=28.7330316742081, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI54607086, Length=313, Percent_Identity=28.1150159744409, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI194018522, Length=320, Percent_Identity=29.375, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194018520, Length=320, Percent_Identity=29.375, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194097354, Length=320, Percent_Identity=29.375, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI46094014, Length=321, Percent_Identity=29.9065420560748, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI4503507, Length=270, Percent_Identity=28.1481481481481, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157426893, Length=303, Percent_Identity=27.0627062706271, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=32.1167883211679, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=75.1898734177215, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=75.1898734177215, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI2367247, Length=374, Percent_Identity=31.283422459893, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI48994988, Length=283, Percent_Identity=30.3886925795053, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1789108, Length=268, Percent_Identity=27.6119402985075, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=51.5, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=389, Percent_Identity=43.9588688946015, Blast_Score=323, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=443, Percent_Identity=31.3769751693002, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=443, Percent_Identity=31.3769751693002, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=429, Percent_Identity=29.1375291375291, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17506081, Length=294, Percent_Identity=28.9115646258503, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=26.7123287671233, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=26.6666666666667, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=351, Percent_Identity=27.0655270655271, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=40.1360544217687, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=291, Percent_Identity=26.4604810996564, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=29.0598290598291, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=67.0854271356784, Blast_Score=535, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=29.2517006802721, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=29.2517006802721, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=30.3834808259587, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322937, Length=352, Percent_Identity=25.8522727272727, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=273, Percent_Identity=26.3736263736264, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=179, Percent_Identity=30.7262569832402, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322359, Length=131, Percent_Identity=32.0610687022901, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=29.0983606557377, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.0330788804071, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.0330788804071, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=49.8701298701299, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=29.4642857142857, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=29.4642857142857, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=29.4642857142857, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=29.4642857142857, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.4642857142857, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.4642857142857, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45550900, Length=343, Percent_Identity=29.4460641399417, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=30.8605341246291, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=30.6666666666667, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=26.3940520446097, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=26.3940520446097, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=31.7567567567568, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42605; Mature: 42474

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSKFERTKPHVNIGTIGHVDHGKTSLTAAITKFFGEFKAYDQIDAAPEERARGITIST
CCCCCCHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEE
AHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKCDQVDDAELLELVELEVRELLSKYEFPGDEIPIIKGSALAALEDSSKE
CCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEECCCHHH
LGEDAIRNLMDAVDSYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHHCCCEE
EIVGIKATTKTTVTGVEMFRKLLDQGQAGDNIGALIRGVGREDVERGQVLCKPGSVKPHT
EEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCEEECCCCCCCCC
KFKAEAYILTKDEGGRHTPFFTNYRPQFYFRTTDVTGVVTLPAGTEMVMPGDNVAMDVTL
CEEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVSSIIE
EEEECHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKSKFERTKPHVNIGTIGHVDHGKTSLTAAITKFFGEFKAYDQIDAAPEERARGITIST
CCCCCHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEE
AHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKCDQVDDAELLELVELEVRELLSKYEFPGDEIPIIKGSALAALEDSSKE
CCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEECCCHHH
LGEDAIRNLMDAVDSYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHHCCCEE
EIVGIKATTKTTVTGVEMFRKLLDQGQAGDNIGALIRGVGREDVERGQVLCKPGSVKPHT
EEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCEEECCCCCCCCC
KFKAEAYILTKDEGGRHTPFFTNYRPQFYFRTTDVTGVVTLPAGTEMVMPGDNVAMDVTL
CEEEEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVSSIIE
EEEECHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA