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The map label for this gene is eno

Identifier: 23502010

GI number: 23502010

Start: 1109682

End: 1110959

Strand: Reverse

Name: eno

Synonym: BR1132

Alternate gene names: 23502010

Gene position: 1110959-1109682 (Counterclockwise)

Preceding gene: 23502011

Following gene: 23502009

Centisome position: 52.71

GC content: 57.43

Gene sequence:

>1278_bases
ATGACTGCAATCATCGACATCGTCGGCCGCGAAATTCTCGACAGCCGCGGCAACCCGACCGTCGAAGTGGATGTCGTGCT
TGAAGATGGCTCTTTCGGACGCGCGGCTGTTCCGTCCGGCGCATCGACTGGCGCGCATGAAGCCGTTGAACTGCGTGATG
GCGGCAGCCGTTATCTTGGCAAGGGCGTGGAAAAGGCAGTTGAAGTAGTCAATGGCAAGATTTTCGACGCCATTGCAGGC
ATGGACGCGGAGAGTCAGCTTCTCATCGACCAGACGCTGATCGATCTTGATGGCTCGGCCAACAAGGGCAACCTCGGCGC
CAATGCTATTCTCGGCGTTTCCCTGGCGGTTGCCAAGGCGGCTGCGCAAGCCAGCGGCCTGCCGCTTTATCGCTATGTGG
GCGGCACCAATGCGCATGTGCTTCCCGTTCCGATGATGAACATCATCAATGGCGGCGCCCATGCCGATAATCCGATCGAT
TTTCAGGAATTCATGATCCTTCCGGTTGGCGCGACTTCCATTCGCGAAGCTGTGCGCTATGGCTCGGAAGTCTTCCACAC
ACTGAAGAAGCGCCTCAAGGATGCCGGACATAACACCAATGTCGGCGACGAAGGCGGCTTTGCGCCAAACCTCAAGAATG
CACAGGCCGCACTCGATTTCATCATGGAATCGATTGAGAAGGCTGGTTTCAAGCCGGGCGAAGATATTGCTCTTGGCCTG
GACTGCGCGGCGACCGAGTTCTTCAAGGACGGCAACTACGTCTATGAAGGCGAGCGCAAGACCCGCGATCCGAAGGCGCA
GGCCAAGTATCTCGCCAAGCTTGCCAGCGACTATCCTATCGTCACCATTGAAGACGGTATGGCTGAAGACGATTGGGAAG
GCTGGAAATATCTGACCGATCTGATCGGCAATAAGTGCCAGCTTGTCGGCGACGATCTGTTCGTGACGAATTCGGCTCGT
CTGCGTGACGGTATCCGTCTGGGCGTCGCCAACTCAATTCTCGTCAAGGTGAACCAGATCGGTTCGCTGTCGGAAACGCT
GGACGCGGTCGAAACCGCCCACAAGGCTGGTTACACCGCCGTTATGTCGCATCGCTCGGGCGAAACGGAAGATTCCACCA
TTGCCGATCTTGCCGTGGCTACCAATTGCGGCCAGATCAAAACCGGCTCGCTCGCGCGTTCGGATCGCACGGCGAAGTAC
AACCAGCTCATCCGCATTGAGGAAGAGCTGGGCAAGCAGGCCCGCTATGCTGGTCGCAGTGCACTGAAGTTGCTCTAA

Upstream 100 bases:

>100_bases
GATGTTTCCATTTGGCTGTAAAATGCTCTAAGGATGTGCCCATATCGGATTTGTTAATGCCTGCCGGCGGCAGGCAATCT
ATAGGAAAGGACTGGCTCCT

Downstream 100 bases:

>100_bases
GCGGAACGGGATTTAGGCAAAAGGCGGGCCACTATGCCCGCCTTTTTTCGTTTTGTTGCTTGGTGTAGTGTGGCAAGCTT
TGTTTAAGTTATCTAGAGCG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTAIIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAG
MDAESQLLIDQTLIDLDGSANKGNLGANAILGVSLAVAKAAAQASGLPLYRYVGGTNAHVLPVPMMNIINGGAHADNPID
FQEFMILPVGATSIREAVRYGSEVFHTLKKRLKDAGHNTNVGDEGGFAPNLKNAQAALDFIMESIEKAGFKPGEDIALGL
DCAATEFFKDGNYVYEGERKTRDPKAQAKYLAKLASDYPIVTIEDGMAEDDWEGWKYLTDLIGNKCQLVGDDLFVTNSAR
LRDGIRLGVANSILVKVNQIGSLSETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKY
NQLIRIEEELGKQARYAGRSALKLL

Sequences:

>Translated_425_residues
MTAIIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAG
MDAESQLLIDQTLIDLDGSANKGNLGANAILGVSLAVAKAAAQASGLPLYRYVGGTNAHVLPVPMMNIINGGAHADNPID
FQEFMILPVGATSIREAVRYGSEVFHTLKKRLKDAGHNTNVGDEGGFAPNLKNAQAALDFIMESIEKAGFKPGEDIALGL
DCAATEFFKDGNYVYEGERKTRDPKAQAKYLAKLASDYPIVTIEDGMAEDDWEGWKYLTDLIGNKCQLVGDDLFVTNSAR
LRDGIRLGVANSILVKVNQIGSLSETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKY
NQLIRIEEELGKQARYAGRSALKLL
>Mature_424_residues
TAIIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLGKGVEKAVEVVNGKIFDAIAGM
DAESQLLIDQTLIDLDGSANKGNLGANAILGVSLAVAKAAAQASGLPLYRYVGGTNAHVLPVPMMNIINGGAHADNPIDF
QEFMILPVGATSIREAVRYGSEVFHTLKKRLKDAGHNTNVGDEGGFAPNLKNAQAALDFIMESIEKAGFKPGEDIALGLD
CAATEFFKDGNYVYEGERKTRDPKAQAKYLAKLASDYPIVTIEDGMAEDDWEGWKYLTDLIGNKCQLVGDDLFVTNSARL
RDGIRLGVANSILVKVNQIGSLSETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYN
QLIRIEEELGKQARYAGRSALKLL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=435, Percent_Identity=51.0344827586207, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI4503571, Length=435, Percent_Identity=49.4252873563218, Blast_Score=400, Evalue=1e-112,
Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=49.4279176201373, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=49.4279176201373, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI301897479, Length=435, Percent_Identity=45.9770114942529, Blast_Score=344, Evalue=9e-95,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=24.4776119402985, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=24.4776119402985, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=24.4776119402985, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=61.3053613053613, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=52.6315789473684, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=52.6315789473684, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563855, Length=194, Percent_Identity=45.8762886597938, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=49.4279176201373, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=48.4988452655889, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=48.4988452655889, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=48.4988452655889, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=48.9702517162471, Blast_Score=366, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=50.4608294930876, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=50.4608294930876, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=50.4608294930876, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=50.4608294930876, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=50.4608294930876, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=50.4608294930876, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_BRUA1 (B2S5Y3)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935054.1
- ProteinModelPortal:   B2S5Y3
- SMR:   B2S5Y3
- GeneID:   6328622
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I10730
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45262; Mature: 45130

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 336-336 BINDING 154-154 BINDING 163-163 BINDING 284-284 BINDING 311-311 BINDING 336-336 BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
KGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLIDLDGSANKGNLGANAILGVSLAVAKA
HHHHHHHHHHCCHHHHHHHCCCCCCCEEHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
AAQASGLPLYRYVGGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRY
HHHHCCCEEEEEECCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHHH
GSEVFHTLKKRLKDAGHNTNVGDEGGFAPNLKNAQAALDFIMESIEKAGFKPGEDIALGL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEC
DCAATEFFKDGNYVYEGERKTRDPKAQAKYLAKLASDYPIVTIEDGMAEDDWEGWKYLTD
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LIGNKCQLVGDDLFVTNSARLRDGIRLGVANSILVKVNQIGSLSETLDAVETAHKAGYTA
HHCCCEEEECCCEEEECCHHHHCCEEECCCCEEEEEEHHCCCHHHHHHHHHHHHHCCCHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYNQLIRIEEELGKQARYAGRS
EEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCCCH
ALKLL
HHHCC
>Mature Secondary Structure 
TAIIDIVGREILDSRGNPTVEVDVVLEDGSFGRAAVPSGASTGAHEAVELRDGGSRYLG
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
KGVEKAVEVVNGKIFDAIAGMDAESQLLIDQTLIDLDGSANKGNLGANAILGVSLAVAKA
HHHHHHHHHHCCHHHHHHHCCCCCCCEEHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
AAQASGLPLYRYVGGTNAHVLPVPMMNIINGGAHADNPIDFQEFMILPVGATSIREAVRY
HHHHCCCEEEEEECCCCCEEECCCHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHHH
GSEVFHTLKKRLKDAGHNTNVGDEGGFAPNLKNAQAALDFIMESIEKAGFKPGEDIALGL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEC
DCAATEFFKDGNYVYEGERKTRDPKAQAKYLAKLASDYPIVTIEDGMAEDDWEGWKYLTD
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
LIGNKCQLVGDDLFVTNSARLRDGIRLGVANSILVKVNQIGSLSETLDAVETAHKAGYTA
HHCCCEEEECCCEEEECCHHHHCCEEECCCCEEEEEEHHCCCHHHHHHHHHHHHHCCCHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRTAKYNQLIRIEEELGKQARYAGRS
EEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCCCH
ALKLL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA