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The map label for this gene is aceF [H]

Identifier: 23502005

GI number: 23502005

Start: 1104219

End: 1105562

Strand: Reverse

Name: aceF [H]

Synonym: BR1127

Alternate gene names: 23502005

Gene position: 1105562-1104219 (Counterclockwise)

Preceding gene: 23502006

Following gene: 23502004

Centisome position: 52.45

GC content: 59.52

Gene sequence:

>1344_bases
ATGCCGATCAATATCACCATGCCAGCGCTTTCTCCCACGATGGAAGAAGGTAACCTGTCGAAATGGCTGGTCAAGGAAGG
CGATAAGGTCGCTCCCGGCGATGTTATTGCCGAAATCGAAACCGACAAGGCCACGATGGAAGTCGAGGCGGTGGACGAGG
GAACGGTCGCCAAGATCGTCGTTCCGGCTGGCACTGAAGGCGTCAAGGTTAATGCGCTGATCGCTGTTCTTGCCGAAGAA
GGTGAGGATGTGGCGGCTGCGGCCAAGGGCGCTGGCGCGGCTCCCAAGGCAGAAGCGCCGAAGGAAGAGCCGAAGCCTGC
TGAAGCGAAAAAGGAAGCTGCGGCTCCTGCCGCTGCACCGGCTCCAGCCAGGAGCGAGCAGCCTGCTGTTGCGCCTGCCG
TAAACAAGGGCGAGCGCGTGTTCGCCTCACCGCTTGCGCGCCGTATCGCCAAGGATGCAGGCGTCGATATTTCTGCCGTG
AAGGGCTCCGGCCCGCATGGCCGTGTGATTCAGCGCGATGTGGAAGCTGCTCTGGCTTCCGGCGGTGCCAAGGCCGTTTC
GGCACAGGCCGAATCTGCTGCCGCGCCGAAGCCGATGTCGGATGATGCCATTCTCAAACTCTTCGAGGACGGCTCCTACG
AGGTCGTGCCGCATGACGGTATGCGCAAGACAATTGCCCGCCGTCTGGTGGAATCGAAGCAGACTGTTCCGCATTTCTAT
CTGACGATCGATTGCGAACTGGATGCGCTTCTGGCGCTGCGTTCGCAGATCAATGCTGCTGCCCCGATGCTCAAGACGGA
AAAGGGCGAGGTTCCGGCCTACAAGCTTTCCGTCAACGACATGGTTATCAAGGCGACGGCGCTGGCGCTGCGTGACGTGC
CGGAGGCGAATGTTTCCTGGACCGAGGGCGGCATGATCAAGCACAAGTGTTCGGATGTGGGGGTTGCCGTTTCCATTCCG
GGCGGCCTGATTACGCCTATCGTCCGCCACGCGGAATCGAAGACGCTCTCCGTTATCTCGAACGAGATGAAGGATATGGC
CAGACGCGCGCGTGACCGCAAGCTGAAGCCTGAGGAATATCAGGGTGGCTCGACCTCCGTGTCAAACCTCGGCATGTTCG
GCGTCAAGGATTTCGCCGCGATCATCAATCCGCCACATGCAACCATTTTCGCAATTGGTGCAGGCGAGGAGCGCGCCGTG
GTCAAGAAGGGGGAAATAAAGGTAGCGACTGTCATGTCTGTTACACTTTCCACCGATCATCGCGCTGTCGATGGTGCGCT
TGCCGCAGAACTTGCGCAGGCATTCAAGCGCCATATCGAAAACCCGATGGGTATGCTGGTCTGA

Upstream 100 bases:

>100_bases
ATGCCGCAAATCTTGAAAAGCTGGCGCTTCCGAGCGTTGCCGAAGTGGTCGAAGCGGTGAAAGCCGTTACCTATACCGCT
TAAACAGAAAGGGTCTGGAC

Downstream 100 bases:

>100_bases
TGGAACTGACGCCGCGCTTGTCTTTGTAGCTTATACGAAGAACAGCGCGGCCTTTCCGTTTTCAGGAATAAGGGCGAAAA
TTATGGCCGACATTTACGAC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MPINITMPALSPTMEEGNLSKWLVKEGDKVAPGDVIAEIETDKATMEVEAVDEGTVAKIVVPAGTEGVKVNALIAVLAEE
GEDVAAAAKGAGAAPKAEAPKEEPKPAEAKKEAAAPAAAPAPARSEQPAVAPAVNKGERVFASPLARRIAKDAGVDISAV
KGSGPHGRVIQRDVEAALASGGAKAVSAQAESAAAPKPMSDDAILKLFEDGSYEVVPHDGMRKTIARRLVESKQTVPHFY
LTIDCELDALLALRSQINAAAPMLKTEKGEVPAYKLSVNDMVIKATALALRDVPEANVSWTEGGMIKHKCSDVGVAVSIP
GGLITPIVRHAESKTLSVISNEMKDMARRARDRKLKPEEYQGGSTSVSNLGMFGVKDFAAIINPPHATIFAIGAGEERAV
VKKGEIKVATVMSVTLSTDHRAVDGALAAELAQAFKRHIENPMGMLV

Sequences:

>Translated_447_residues
MPINITMPALSPTMEEGNLSKWLVKEGDKVAPGDVIAEIETDKATMEVEAVDEGTVAKIVVPAGTEGVKVNALIAVLAEE
GEDVAAAAKGAGAAPKAEAPKEEPKPAEAKKEAAAPAAAPAPARSEQPAVAPAVNKGERVFASPLARRIAKDAGVDISAV
KGSGPHGRVIQRDVEAALASGGAKAVSAQAESAAAPKPMSDDAILKLFEDGSYEVVPHDGMRKTIARRLVESKQTVPHFY
LTIDCELDALLALRSQINAAAPMLKTEKGEVPAYKLSVNDMVIKATALALRDVPEANVSWTEGGMIKHKCSDVGVAVSIP
GGLITPIVRHAESKTLSVISNEMKDMARRARDRKLKPEEYQGGSTSVSNLGMFGVKDFAAIINPPHATIFAIGAGEERAV
VKKGEIKVATVMSVTLSTDHRAVDGALAAELAQAFKRHIENPMGMLV
>Mature_446_residues
PINITMPALSPTMEEGNLSKWLVKEGDKVAPGDVIAEIETDKATMEVEAVDEGTVAKIVVPAGTEGVKVNALIAVLAEEG
EDVAAAAKGAGAAPKAEAPKEEPKPAEAKKEAAAPAAAPAPARSEQPAVAPAVNKGERVFASPLARRIAKDAGVDISAVK
GSGPHGRVIQRDVEAALASGGAKAVSAQAESAAAPKPMSDDAILKLFEDGSYEVVPHDGMRKTIARRLVESKQTVPHFYL
TIDCELDALLALRSQINAAAPMLKTEKGEVPAYKLSVNDMVIKATALALRDVPEANVSWTEGGMIKHKCSDVGVAVSIPG
GLITPIVRHAESKTLSVISNEMKDMARRARDRKLKPEEYQGGSTSVSNLGMFGVKDFAAIINPPHATIFAIGAGEERAVV
KKGEIKVATVMSVTLSTDHRAVDGALAAELAQAFKRHIENPMGMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=450, Percent_Identity=41.1111111111111, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI203098753, Length=484, Percent_Identity=35.9504132231405, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI203098816, Length=484, Percent_Identity=35.9504132231405, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=43.0379746835443, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI110671329, Length=444, Percent_Identity=26.3513513513513, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=29.2576419213974, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1786946, Length=445, Percent_Identity=29.438202247191, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786305, Length=419, Percent_Identity=30.5489260143198, Blast_Score=139, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=455, Percent_Identity=43.2967032967033, Blast_Score=321, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI17538894, Length=313, Percent_Identity=34.8242811501597, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=439, Percent_Identity=27.5626423690205, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=457, Percent_Identity=28.8840262582057, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=41.304347826087, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6320352, Length=444, Percent_Identity=27.2522522522523, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321632, Length=82, Percent_Identity=45.1219512195122, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI20129315, Length=456, Percent_Identity=41.2280701754386, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24582497, Length=445, Percent_Identity=40.6741573033708, Blast_Score=268, Evalue=7e-72,
Organism=Drosophila melanogaster, GI18859875, Length=453, Percent_Identity=27.1523178807947, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24645909, Length=241, Percent_Identity=29.8755186721992, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46735; Mature: 46604

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPINITMPALSPTMEEGNLSKWLVKEGDKVAPGDVIAEIETDKATMEVEAVDEGTVAKIV
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPAGTEGVKVNALIAVLAEEGEDVAAAAKGAGAAPKAEAPKEEPKPAEAKKEAAAPAAAP
EECCCCCEEHHEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
APARSEQPAVAPAVNKGERVFASPLARRIAKDAGVDISAVKGSGPHGRVIQRDVEAALAS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHC
GGAKAVSAQAESAAAPKPMSDDAILKLFEDGSYEVVPHDGMRKTIARRLVESKQTVPHFY
CCCHHHHCCHHHCCCCCCCCCHHHHEEEECCCEEEECCCCHHHHHHHHHHHHCCCCCEEE
LTIDCELDALLALRSQINAAAPMLKTEKGEVPAYKLSVNDMVIKATALALRDVPEANVSW
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHCCCCCCCCC
TEGGMIKHKCSDVGVAVSIPGGLITPIVRHAESKTLSVISNEMKDMARRARDRKLKPEEY
CCCCEEEEECCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHC
QGGSTSVSNLGMFGVKDFAAIINPPHATIFAIGAGEERAVVKKGEIKVATVMSVTLSTDH
CCCCCCHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCCEEEECCCEEEEEEEEEEECCCC
RAVDGALAAELAQAFKRHIENPMGMLV
HHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PINITMPALSPTMEEGNLSKWLVKEGDKVAPGDVIAEIETDKATMEVEAVDEGTVAKIV
CEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPAGTEGVKVNALIAVLAEEGEDVAAAAKGAGAAPKAEAPKEEPKPAEAKKEAAAPAAAP
EECCCCCEEHHEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
APARSEQPAVAPAVNKGERVFASPLARRIAKDAGVDISAVKGSGPHGRVIQRDVEAALAS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHC
GGAKAVSAQAESAAAPKPMSDDAILKLFEDGSYEVVPHDGMRKTIARRLVESKQTVPHFY
CCCHHHHCCHHHCCCCCCCCCHHHHEEEECCCEEEECCCCHHHHHHHHHHHHCCCCCEEE
LTIDCELDALLALRSQINAAAPMLKTEKGEVPAYKLSVNDMVIKATALALRDVPEANVSW
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHCCCCCCCCC
TEGGMIKHKCSDVGVAVSIPGGLITPIVRHAESKTLSVISNEMKDMARRARDRKLKPEEY
CCCCEEEEECCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHC
QGGSTSVSNLGMFGVKDFAAIINPPHATIFAIGAGEERAVVKKGEIKVATVMSVTLSTDH
CCCCCCHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCCEEEECCCEEEEEEEEEEECCCC
RAVDGALAAELAQAFKRHIENPMGMLV
HHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]