Click here to switch to the map view.

The map label for this gene is lipA

Identifier: 23502002

GI number: 23502002

Start: 1101315

End: 1102283

Strand: Reverse

Name: lipA

Synonym: BR1124

Alternate gene names: 23502002

Gene position: 1102283-1101315 (Counterclockwise)

Preceding gene: 23502003

Following gene: 23502001

Centisome position: 52.3

GC content: 56.45

Gene sequence:

>969_bases
ATGGTTACAGTTCTCAATACGGTCAACCAGAGCGGACGTTTGCGGCACCCGGAAAAGGCACACCGCCCCGACAATGAGGT
GCTGAAAAAGCCGGACTGGATTCGCGTCAAGGCGCCTGTTTCGCGCGGCTATGGCGAAACGCGCGAGATTGTCCGCTCGA
ACAAGCTTGTGACTGTCTGTGAAGAAGCCGGTTGCCCGAATATCGGCGAGTGCTGGGAAAAGAAGCACGCGACTTTCATG
ATTATGGGCGAAATCTGCACACGCGCCTGCGCGTTCTGCAATATTTCGACCGGCATTCCGAATGCGCTCGACCCGAACGA
GCCGGAAAATATTGCCAAGGCCGTGAAGCAGATGGGGCTGACCCATGTGGTCATTACCTCGGTGGACCGTGACGATCTGG
CCGATGGCGGTGCGCATCATTTTGCCGAAGTTATCAAGGCCGTGCGCGAGGCCGCACCGGCGACCACGATCGAGATTCTG
ACACCGGACTTCCTGCGCAAGGAAGGCGCGCTGGAGATCGTGGTCAAGGCTCGCCCTGACGTGTTCAACCACAATCTCGA
AACAGTGCCGTCGAAATATCTCAAGGTTCGTCCGGGTGCGCGCTATTTCCACTCCATCCGCCTGCTTCAGCGTGTGAAGG
AACTGGATCCGACCATTTTCACCAAGTCCGGCATTATGGTTGGCCTGGGCGAAGAGCGGAACGAAATACTTCAGCTTATG
GACGATCTGCGCTCTGCTGATGTTGATTTCATGACTATCGGCCAATATCTTCAGCCCACCCGCAAGCATCATCCGGTTAT
CCGCTTCGTCAAGCCCGATGAATTCAAGTCTTTCGAGACCATCGGCAAGACAAAGGGCTTCCTGCTGGTCGCTTCCAGCC
CGCTGACACGCTCGTCGCATCATGCGGGTGAAGATTTTGCCAAGCTCAAGGCGGCGCGCGAAGCGCTTTATGCTTCAAGG
GCCTCGTAA

Upstream 100 bases:

>100_bases
GCTCAAGTTGACAGGGCATGGGCTTGAGATTAGGACCGGACCAAATTTAGGAGCATTTCAGCCCCATCGGGGATGGTGCT
TCGGATAGGCAGGAAACAGC

Downstream 100 bases:

>100_bases
GCGGAATGCCTCAATTTACGACCGCCAGGCGTGCTCGCCACCGGGCGGAACAGATGTTTGCGCTCGTTGCGGATGTTGAG
AAATATCCGCAATTCCTGCC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MVTVLNTVNQSGRLRHPEKAHRPDNEVLKKPDWIRVKAPVSRGYGETREIVRSNKLVTVCEEAGCPNIGECWEKKHATFM
IMGEICTRACAFCNISTGIPNALDPNEPENIAKAVKQMGLTHVVITSVDRDDLADGGAHHFAEVIKAVREAAPATTIEIL
TPDFLRKEGALEIVVKARPDVFNHNLETVPSKYLKVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGEERNEILQLM
DDLRSADVDFMTIGQYLQPTRKHHPVIRFVKPDEFKSFETIGKTKGFLLVASSPLTRSSHHAGEDFAKLKAAREALYASR
AS

Sequences:

>Translated_322_residues
MVTVLNTVNQSGRLRHPEKAHRPDNEVLKKPDWIRVKAPVSRGYGETREIVRSNKLVTVCEEAGCPNIGECWEKKHATFM
IMGEICTRACAFCNISTGIPNALDPNEPENIAKAVKQMGLTHVVITSVDRDDLADGGAHHFAEVIKAVREAAPATTIEIL
TPDFLRKEGALEIVVKARPDVFNHNLETVPSKYLKVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGEERNEILQLM
DDLRSADVDFMTIGQYLQPTRKHHPVIRFVKPDEFKSFETIGKTKGFLLVASSPLTRSSHHAGEDFAKLKAAREALYASR
AS
>Mature_322_residues
MVTVLNTVNQSGRLRHPEKAHRPDNEVLKKPDWIRVKAPVSRGYGETREIVRSNKLVTVCEEAGCPNIGECWEKKHATFM
IMGEICTRACAFCNISTGIPNALDPNEPENIAKAVKQMGLTHVVITSVDRDDLADGGAHHFAEVIKAVREAAPATTIEIL
TPDFLRKEGALEIVVKARPDVFNHNLETVPSKYLKVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGEERNEILQLM
DDLRSADVDFMTIGQYLQPTRKHHPVIRFVKPDEFKSFETIGKTKGFLLVASSPLTRSSHHAGEDFAKLKAAREALYASR
AS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=45.2380952380952, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI37577164, Length=252, Percent_Identity=45.2380952380952, Blast_Score=229, Evalue=2e-60,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=52.158273381295, Blast_Score=294, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=45.7364341085271, Blast_Score=237, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=296, Percent_Identity=44.5945945945946, Blast_Score=262, Evalue=6e-71,
Organism=Drosophila melanogaster, GI221513272, Length=309, Percent_Identity=42.3948220064725, Blast_Score=254, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_BRUA1 (B2S5X5)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935046.1
- ProteinModelPortal:   B2S5X5
- GeneID:   6328614
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I10650
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35958; Mature: 35958

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVLNTVNQSGRLRHPEKAHRPDNEVLKKPDWIRVKAPVSRGYGETREIVRSNKLVTVC
CEEEEECCCCCCCCCCCHHHCCCCHHHHCCCCCEEEECCHHCCCCHHHHHHHCCCEEEEE
EEAGCPNIGECWEKKHATFMIMGEICTRACAFCNISTGIPNALDPNEPENIAKAVKQMGL
CCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCC
THVVITSVDRDDLADGGAHHFAEVIKAVREAAPATTIEILTPDFLRKEGALEIVVKARPD
EEEEEEECCHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCEEEEEECCCC
VFNHNLETVPSKYLKVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGEERNEILQLM
HHCCCHHHCCHHHEEECCCHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHH
DDLRSADVDFMTIGQYLQPTRKHHPVIRFVKPDEFKSFETIGKTKGFLLVASSPLTRSSH
HHHHHCCCCHHHHHHHHCHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCCCCCC
HAGEDFAKLKAAREALYASRAS
CCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVTVLNTVNQSGRLRHPEKAHRPDNEVLKKPDWIRVKAPVSRGYGETREIVRSNKLVTVC
CEEEEECCCCCCCCCCCHHHCCCCHHHHCCCCCEEEECCHHCCCCHHHHHHHCCCEEEEE
EEAGCPNIGECWEKKHATFMIMGEICTRACAFCNISTGIPNALDPNEPENIAKAVKQMGL
CCCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCC
THVVITSVDRDDLADGGAHHFAEVIKAVREAAPATTIEILTPDFLRKEGALEIVVKARPD
EEEEEEECCHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCEEEEEECCCC
VFNHNLETVPSKYLKVRPGARYFHSIRLLQRVKELDPTIFTKSGIMVGLGEERNEILQLM
HHCCCHHHCCHHHEEECCCHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCHHHHHHHH
DDLRSADVDFMTIGQYLQPTRKHHPVIRFVKPDEFKSFETIGKTKGFLLVASSPLTRSSH
HHHHHCCCCHHHHHHHHCHHHHCCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCCCCCC
HAGEDFAKLKAAREALYASRAS
CCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA