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The map label for this gene is pbpF [H]

Identifier: 23501472

GI number: 23501472

Start: 575508

End: 577664

Strand: Reverse

Name: pbpF [H]

Synonym: BR0583

Alternate gene names: 23501472

Gene position: 577664-575508 (Counterclockwise)

Preceding gene: 23501502

Following gene: 23501471

Centisome position: 27.41

GC content: 59.16

Gene sequence:

>2157_bases
ATGCGTAATACGGCAGAAAAAGATCGCAGAAACGACAAGCGTAAACCCTTCCGGGTTTCGCGCCTGATCGGACTCGACGC
CTGGATTGATTCCGCGCTCTACAATTTACATTTCCGCTTGGGCGAATGGTGGGAAAACATCACGATTTTCTCCCGGCGTT
TTCGCGTGCGCGGCTTTCGCCGTTTCACGGTGGAAGTGCTGGACGAAGGCTTTACGCTCGGCGTCGCCGGTTCCGTGCTG
ATGTTGATGCTGGCGCTGCCAGCCTTTGAGGAAACCAAGAAGGACTGGCGCGCGCAGGACGATTATGCCGTCACCTTTCT
TGACCGTTATGGCAACGAGATTGGCCGGCGCGGTATTCTGCACCGGCAGGCGGTGCCTATCGATGAATTGCCGGATCATG
TCATCAAGGCTGTGCTCGGCACCGAGGACCGTCGCTTCTTCGACCATTACGGCATTGATCTCATGGGGCTTAGCCGAGCG
TTAAGCCAGAACATGCGCGCCAACGGCGTGGTTCAGGGCGGCTCGACCATCACGCAGCAGCTTGCCAAAAACCTGTTTCT
TTCCAATGAGCGCACCATCGAACGCAAGGTCAAGGAAGCCTTTCTCGCGCTCTGGCTTGAAAGCAATCTCAGCAAGAAGA
AAATCCTCCAGCTTTACCTCGACCGTGCCTATATGGGCGGCGGCACTTTCGGCATTGCAGCCGCGTCCGAGTTTTATTTC
GGCAAGAATGTAAAGGACATCTCGCTGGCCGAGGCAGCCATGCTGGCCGGCCTGTTCAAGGCTCCGGCGAAATTCGCGCC
GCACGTCAACCTGCCTGCAGCTCGCGCCCGCGCCAATGTCGTTCTCTCCAACATGGTGGAAAGCGGCTTTCTCAGCGAAG
GTCAGGTTGCCGTCGCGCGCCGCCACCCGGCCAGCGTCATAGATCGCGCCAAGGATGAAAGCCCCGACTATTTCCTGGAC
TGGGCCTTCGACGAGGTGAAAAAGGTTGCAGACAGGTTCAACCAGCATACGCTGATCGTGCGCACCACGCTTGACCGCAA
TATCCAGAAAGCCGCCGAAGAATCGCTTGAATTTCACCTTCGCCAATATGGCAAGGAATATAACGTATCGGAGGCGGCCA
CCGTCGTGCTTGCCAATGACGGTTCCGTGCGCGCGCTCGTTGGCGGGCGCGACTATGGCGAAAGCCAGTTCAACCGCGCA
ACCAGGGCGCTGCGGCAGGCCGGTTCATCCTTCAAGCCCTATGTCTATGCGGCTGCCATGGAAAAGGGGCTGACACCAAG
CACCATCGTTTCCGATGCACCGATCAGCTGGGGCAACTGGTCGCCGCGCAATTACGGGCGCAGTTTTGCTGGCCGGGTTG
ATCTCACCACCGCGCTCGTCCGCTCGCTCAACAGCGTGCCGGTGCGCCTTGCCCGCGATTATCTGACCACGGCGCCCGTT
GTCGCGCTCACAAAGGCAATGGGTGTGGAATCGCCCATCTCCTCCCATAAGACGATGGTGCTCGGCACATCCGAAATGAC
CGTCATGGATCAGGCAACAGGCTTTAACGTCTTCGCCAATGCGGGCATGGCCGGAAACCGTCACGCTTTCACGCAAATCC
TGGCATCCGACGGAAAGGTTCTTTGGGATTTTGGCCGTGACGCACCAAAGCCGCACCGCGCCCTTTCGGAAAAGGCCGCA
CTTGAGATGAACTCCATGCTGGTGCAGGTGCCCGAGCGCGGCACGGGCCGGCGCGCCGCACTTACGATGACGCGCGTGGC
AGGCAAAACGGGTACGACCCAGAACTATCGCGATGCCTGGTTCGTGGGCTTTACCGGAAATTTCACCGCCGCCGTCTGGT
TCGGCAACGATAATTTCACGCCCATGAAGGAACTGACCGGCGGTGTTCTGCCAGCCATGGCCTGGCAGCGCATGATGGCA
TACGCCCATCAGAATATCGAATTGAAGCCGCTGCCGGGCGTCAACCCGCCCTTCCCCGCCCAGCCGAAGAACCCGCCCGC
ACAGGTGGCCGATACGAGGCCGCACGAAACAATGGCTGCCCCACCACGGGTTTTATCGCCGCTGGCGACAAAAATTCTCA
AGGAACTGCATGACCGCTTCCTTGCCGCGCCGCCTCTGCCTACGATAGCCGAGCGCACAAAAGTTTCGGTACTCTGA

Upstream 100 bases:

>100_bases
TTTCATTTAGCTATTTTGCGTGAGCTAGCTTATTTATCTGTTATGATAATGAGGCAAATCTCGCCTTGTCTCCGACAACC
CTATGCCGAAGTCAGTGTTC

Downstream 100 bases:

>100_bases
GGGGCGATGTTCAAAACGATTTTCAAGGTAGTGGCTGTGCTTGCGCTCGCGCTTGGCGGGGGCATCTGGAGCGTGGATTA
CGCACTGGACAGTTTTGAAG

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MRNTAEKDRRNDKRKPFRVSRLIGLDAWIDSALYNLHFRLGEWWENITIFSRRFRVRGFRRFTVEVLDEGFTLGVAGSVL
MLMLALPAFEETKKDWRAQDDYAVTFLDRYGNEIGRRGILHRQAVPIDELPDHVIKAVLGTEDRRFFDHYGIDLMGLSRA
LSQNMRANGVVQGGSTITQQLAKNLFLSNERTIERKVKEAFLALWLESNLSKKKILQLYLDRAYMGGGTFGIAAASEFYF
GKNVKDISLAEAAMLAGLFKAPAKFAPHVNLPAARARANVVLSNMVESGFLSEGQVAVARRHPASVIDRAKDESPDYFLD
WAFDEVKKVADRFNQHTLIVRTTLDRNIQKAAEESLEFHLRQYGKEYNVSEAATVVLANDGSVRALVGGRDYGESQFNRA
TRALRQAGSSFKPYVYAAAMEKGLTPSTIVSDAPISWGNWSPRNYGRSFAGRVDLTTALVRSLNSVPVRLARDYLTTAPV
VALTKAMGVESPISSHKTMVLGTSEMTVMDQATGFNVFANAGMAGNRHAFTQILASDGKVLWDFGRDAPKPHRALSEKAA
LEMNSMLVQVPERGTGRRAALTMTRVAGKTGTTQNYRDAWFVGFTGNFTAAVWFGNDNFTPMKELTGGVLPAMAWQRMMA
YAHQNIELKPLPGVNPPFPAQPKNPPAQVADTRPHETMAAPPRVLSPLATKILKELHDRFLAAPPLPTIAERTKVSVL

Sequences:

>Translated_718_residues
MRNTAEKDRRNDKRKPFRVSRLIGLDAWIDSALYNLHFRLGEWWENITIFSRRFRVRGFRRFTVEVLDEGFTLGVAGSVL
MLMLALPAFEETKKDWRAQDDYAVTFLDRYGNEIGRRGILHRQAVPIDELPDHVIKAVLGTEDRRFFDHYGIDLMGLSRA
LSQNMRANGVVQGGSTITQQLAKNLFLSNERTIERKVKEAFLALWLESNLSKKKILQLYLDRAYMGGGTFGIAAASEFYF
GKNVKDISLAEAAMLAGLFKAPAKFAPHVNLPAARARANVVLSNMVESGFLSEGQVAVARRHPASVIDRAKDESPDYFLD
WAFDEVKKVADRFNQHTLIVRTTLDRNIQKAAEESLEFHLRQYGKEYNVSEAATVVLANDGSVRALVGGRDYGESQFNRA
TRALRQAGSSFKPYVYAAAMEKGLTPSTIVSDAPISWGNWSPRNYGRSFAGRVDLTTALVRSLNSVPVRLARDYLTTAPV
VALTKAMGVESPISSHKTMVLGTSEMTVMDQATGFNVFANAGMAGNRHAFTQILASDGKVLWDFGRDAPKPHRALSEKAA
LEMNSMLVQVPERGTGRRAALTMTRVAGKTGTTQNYRDAWFVGFTGNFTAAVWFGNDNFTPMKELTGGVLPAMAWQRMMA
YAHQNIELKPLPGVNPPFPAQPKNPPAQVADTRPHETMAAPPRVLSPLATKILKELHDRFLAAPPLPTIAERTKVSVL
>Mature_718_residues
MRNTAEKDRRNDKRKPFRVSRLIGLDAWIDSALYNLHFRLGEWWENITIFSRRFRVRGFRRFTVEVLDEGFTLGVAGSVL
MLMLALPAFEETKKDWRAQDDYAVTFLDRYGNEIGRRGILHRQAVPIDELPDHVIKAVLGTEDRRFFDHYGIDLMGLSRA
LSQNMRANGVVQGGSTITQQLAKNLFLSNERTIERKVKEAFLALWLESNLSKKKILQLYLDRAYMGGGTFGIAAASEFYF
GKNVKDISLAEAAMLAGLFKAPAKFAPHVNLPAARARANVVLSNMVESGFLSEGQVAVARRHPASVIDRAKDESPDYFLD
WAFDEVKKVADRFNQHTLIVRTTLDRNIQKAAEESLEFHLRQYGKEYNVSEAATVVLANDGSVRALVGGRDYGESQFNRA
TRALRQAGSSFKPYVYAAAMEKGLTPSTIVSDAPISWGNWSPRNYGRSFAGRVDLTTALVRSLNSVPVRLARDYLTTAPV
VALTKAMGVESPISSHKTMVLGTSEMTVMDQATGFNVFANAGMAGNRHAFTQILASDGKVLWDFGRDAPKPHRALSEKAA
LEMNSMLVQVPERGTGRRAALTMTRVAGKTGTTQNYRDAWFVGFTGNFTAAVWFGNDNFTPMKELTGGVLPAMAWQRMMA
YAHQNIELKPLPGVNPPFPAQPKNPPAQVADTRPHETMAAPPRVLSPLATKILKELHDRFLAAPPLPTIAERTKVSVL

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=541, Percent_Identity=34.0110905730129, Blast_Score=245, Evalue=8e-66,
Organism=Escherichia coli, GI1788867, Length=521, Percent_Identity=31.2859884836852, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87082258, Length=288, Percent_Identity=36.4583333333333, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1789601, Length=183, Percent_Identity=36.6120218579235, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79815; Mature: 79815

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNTAEKDRRNDKRKPFRVSRLIGLDAWIDSALYNLHFRLGEWWENITIFSRRFRVRGFR
CCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFTVEVLDEGFTLGVAGSVLMLMLALPAFEETKKDWRAQDDYAVTFLDRYGNEIGRRGIL
HHHHHHHHCCCEEHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCH
HRQAVPIDELPDHVIKAVLGTEDRRFFDHYGIDLMGLSRALSQNMRANGVVQGGSTITQQ
HCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCEECCCHHHHHH
LAKNLFLSNERTIERKVKEAFLALWLESNLSKKKILQLYLDRAYMGGGTFGIAAASEFYF
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GKNVKDISLAEAAMLAGLFKAPAKFAPHVNLPAARARANVVLSNMVESGFLSEGQVAVAR
CCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEC
RHPASVIDRAKDESPDYFLDWAFDEVKKVADRFNQHTLIVRTTLDRNIQKAAEESLEFHL
CCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHH
RQYGKEYNVSEAATVVLANDGSVRALVGGRDYGESQFNRATRALRQAGSSFKPYVYAAAM
HHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHH
EKGLTPSTIVSDAPISWGNWSPRNYGRSFAGRVDLTTALVRSLNSVPVRLARDYLTTAPV
HCCCCCHHEECCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VALTKAMGVESPISSHKTMVLGTSEMTVMDQATGFNVFANAGMAGNRHAFTQILASDGKV
HHHHHHHCCCCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCE
LWDFGRDAPKPHRALSEKAALEMNSMLVQVPERGTGRRAALTMTRVAGKTGTTQNYRDAW
EEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHEEHHHHCCCCCCCCCCCCEE
FVGFTGNFTAAVWFGNDNFTPMKELTGGVLPAMAWQRMMAYAHQNIELKPLPGVNPPFPA
EEEEECCEEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
QPKNPPAQVADTRPHETMAAPPRVLSPLATKILKELHDRFLAAPPLPTIAERTKVSVL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MRNTAEKDRRNDKRKPFRVSRLIGLDAWIDSALYNLHFRLGEWWENITIFSRRFRVRGFR
CCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFTVEVLDEGFTLGVAGSVLMLMLALPAFEETKKDWRAQDDYAVTFLDRYGNEIGRRGIL
HHHHHHHHCCCEEHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCH
HRQAVPIDELPDHVIKAVLGTEDRRFFDHYGIDLMGLSRALSQNMRANGVVQGGSTITQQ
HCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCEECCCHHHHHH
LAKNLFLSNERTIERKVKEAFLALWLESNLSKKKILQLYLDRAYMGGGTFGIAAASEFYF
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GKNVKDISLAEAAMLAGLFKAPAKFAPHVNLPAARARANVVLSNMVESGFLSEGQVAVAR
CCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEC
RHPASVIDRAKDESPDYFLDWAFDEVKKVADRFNQHTLIVRTTLDRNIQKAAEESLEFHL
CCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHH
RQYGKEYNVSEAATVVLANDGSVRALVGGRDYGESQFNRATRALRQAGSSFKPYVYAAAM
HHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHH
EKGLTPSTIVSDAPISWGNWSPRNYGRSFAGRVDLTTALVRSLNSVPVRLARDYLTTAPV
HCCCCCHHEECCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VALTKAMGVESPISSHKTMVLGTSEMTVMDQATGFNVFANAGMAGNRHAFTQILASDGKV
HHHHHHHCCCCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCE
LWDFGRDAPKPHRALSEKAALEMNSMLVQVPERGTGRRAALTMTRVAGKTGTTQNYRDAW
EEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHEEHHHHCCCCCCCCCCCCEE
FVGFTGNFTAAVWFGNDNFTPMKELTGGVLPAMAWQRMMAYAHQNIELKPLPGVNPPFPA
EEEEECCEEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
QPKNPPAQVADTRPHETMAAPPRVLSPLATKILKELHDRFLAAPPLPTIAERTKVSVL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]