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The map label for this gene is rfbE [H]

Identifier: 23501414

GI number: 23501414

Start: 518244

End: 519002

Strand: Reverse

Name: rfbE [H]

Synonym: BR0519

Alternate gene names: 23501414

Gene position: 519002-518244 (Counterclockwise)

Preceding gene: 23501415

Following gene: 23501413

Centisome position: 24.62

GC content: 48.75

Gene sequence:

>759_bases
ATGATCCAGCCATCGATTACCCTGTCAAATGTTCATCTGCACTACGCTGCATCAGCGTTCAAAGAACGCTCAGTCAAAAC
TCTAGTAAACGCCTTATTGAGTATGCGGCGCAGCGCAGGAGTAAACATTGAAGACATCCATGCCCTAAAGGGTATTTCTG
TAGATATAGCGCGGGGCGAACGCGTTGCCCTGATAGGTCACAACGGGGCTGGCAAAAGTACGTTCTTGAAAACTATAGCC
GGTCTCTACCCTATATCATCTGGGACATTAAAAGTGACGGGTACCGTAAGATCCCTGTTCGATATTGGTCTTGGGTTTGA
GCCTGATGCAACTGGCCGTGAGAATATTCTTTACCGTGGGTTGCTTCTCGGACTAACGCCACGTTTCATGCGAGAGATCG
AGGATGAGATCATCGAGTTCGCGGATCTCGGCGATTTTATCGATTATCCAATCAAAACTTATTCTGCCGGCATGCAAGTT
CGGCTCGCCTTCGCGATTTCGACAGCAGTCGACGGCGACATACTCCTTCTAGACGAAGTTATAGGTGCAGGTGATGCGGC
ATTCATGACTAAGGCGAAGGCCCGCATAATGAATATGGTCGAGAAGGCTGAGATAATGGTTCTAGCAAGCCATGACCTTG
CGAACGTCCGTCAGCTTTGCACACGAGCATTGGTTTTCAAAGCCGGCACAATTGCATTTGATGGCAGGGTAGAAGACGCG
ATTTCCTTCTATAACTCGGGAATGGGAGCTATAGCATGA

Upstream 100 bases:

>100_bases
CGGCTACCAATTACATTTGGTGCTTAGGTGTGAGCCTCCTCCTAACCTGCGTGGCAGTAGCTGTGGGGATGCGTGCGGAG
AAGAGAGCCATTTTTTACCT

Downstream 100 bases:

>100_bases
TAAGAGATTCAGGCGCTCCAAGACGTGTTTTATGGTTGTTAAATCATACTACTTTACGAGAATGTGAGGTCCCTCTTCTT
CAGAGTATGGGGTTTGAGAT

Product: O-antigen export system ATP-binding protein RfbE

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MIQPSITLSNVHLHYAASAFKERSVKTLVNALLSMRRSAGVNIEDIHALKGISVDIARGERVALIGHNGAGKSTFLKTIA
GLYPISSGTLKVTGTVRSLFDIGLGFEPDATGRENILYRGLLLGLTPRFMREIEDEIIEFADLGDFIDYPIKTYSAGMQV
RLAFAISTAVDGDILLLDEVIGAGDAAFMTKAKARIMNMVEKAEIMVLASHDLANVRQLCTRALVFKAGTIAFDGRVEDA
ISFYNSGMGAIA

Sequences:

>Translated_252_residues
MIQPSITLSNVHLHYAASAFKERSVKTLVNALLSMRRSAGVNIEDIHALKGISVDIARGERVALIGHNGAGKSTFLKTIA
GLYPISSGTLKVTGTVRSLFDIGLGFEPDATGRENILYRGLLLGLTPRFMREIEDEIIEFADLGDFIDYPIKTYSAGMQV
RLAFAISTAVDGDILLLDEVIGAGDAAFMTKAKARIMNMVEKAEIMVLASHDLANVRQLCTRALVFKAGTIAFDGRVEDA
ISFYNSGMGAIA
>Mature_252_residues
MIQPSITLSNVHLHYAASAFKERSVKTLVNALLSMRRSAGVNIEDIHALKGISVDIARGERVALIGHNGAGKSTFLKTIA
GLYPISSGTLKVTGTVRSLFDIGLGFEPDATGRENILYRGLLLGLTPRFMREIEDEIIEFADLGDFIDYPIKTYSAGMQV
RLAFAISTAVDGDILLLDEVIGAGDAAFMTKAKARIMNMVEKAEIMVLASHDLANVRQLCTRALVFKAGTIAFDGRVEDA
ISFYNSGMGAIA

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbA [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=204, Percent_Identity=31.8627450980392, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI150417984, Length=197, Percent_Identity=28.9340101522843, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI27477115, Length=184, Percent_Identity=27.1739130434783, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI153792144, Length=253, Percent_Identity=24.901185770751, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI27262624, Length=268, Percent_Identity=25.3731343283582, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI27262626, Length=268, Percent_Identity=25.3731343283582, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI27436953, Length=245, Percent_Identity=24.8979591836735, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI105990541, Length=211, Percent_Identity=27.4881516587678, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1788761, Length=201, Percent_Identity=31.8407960199005, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790467, Length=198, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787792, Length=219, Percent_Identity=30.1369863013699, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787029, Length=206, Percent_Identity=27.6699029126214, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787105, Length=199, Percent_Identity=28.1407035175879, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081791, Length=207, Percent_Identity=28.0193236714976, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787712, Length=198, Percent_Identity=25.7575757575758, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786703, Length=214, Percent_Identity=27.1028037383178, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081709, Length=202, Percent_Identity=28.2178217821782, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787758, Length=263, Percent_Identity=22.4334600760456, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1786253, Length=201, Percent_Identity=25.3731343283582, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790525, Length=220, Percent_Identity=25.9090909090909, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193202349, Length=225, Percent_Identity=28, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17567269, Length=215, Percent_Identity=29.3023255813954, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115535101, Length=258, Percent_Identity=25.968992248062, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=217, Percent_Identity=30.8755760368664, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=218, Percent_Identity=27.0642201834862, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=235, Percent_Identity=25.1063829787234, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17567265, Length=217, Percent_Identity=27.6497695852535, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI116007184, Length=185, Percent_Identity=25.9459459459459, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221500365, Length=202, Percent_Identity=25.7425742574257, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221512771, Length=211, Percent_Identity=28.9099526066351, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24643674, Length=217, Percent_Identity=26.7281105990783, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648314, Length=197, Percent_Identity=27.4111675126904, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 27171; Mature: 27171

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQPSITLSNVHLHYAASAFKERSVKTLVNALLSMRRSAGVNIEDIHALKGISVDIARGE
CCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCCC
RVALIGHNGAGKSTFLKTIAGLYPISSGTLKVTGTVRSLFDIGLGFEPDATGRENILYRG
EEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHH
LLLGLTPRFMREIEDEIIEFADLGDFIDYPIKTYSAGMQVRLAFAISTAVDGDILLLDEV
HHHHCCHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCCCEEEEEEEEEHHCCCCEEEEEHH
IGAGDAAFMTKAKARIMNMVEKAEIMVLASHDLANVRQLCTRALVFKAGTIAFDGRVEDA
HCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHCCCEEECCCHHHH
ISFYNSGMGAIA
HHHHHCCCCCCC
>Mature Secondary Structure
MIQPSITLSNVHLHYAASAFKERSVKTLVNALLSMRRSAGVNIEDIHALKGISVDIARGE
CCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECCCC
RVALIGHNGAGKSTFLKTIAGLYPISSGTLKVTGTVRSLFDIGLGFEPDATGRENILYRG
EEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCHHHHHHH
LLLGLTPRFMREIEDEIIEFADLGDFIDYPIKTYSAGMQVRLAFAISTAVDGDILLLDEV
HHHHCCHHHHHHHHHHHHHHHHCCHHHHCCHHHHCCCCEEEEEEEEEHHCCCCEEEEEHH
IGAGDAAFMTKAKARIMNMVEKAEIMVLASHDLANVRQLCTRALVFKAGTIAFDGRVEDA
HCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHCCCEEECCCHHHH
ISFYNSGMGAIA
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8626291 [H]