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The map label for this gene is purD [H]

Identifier: 23501317

GI number: 23501317

Start: 418974

End: 420257

Strand: Reverse

Name: purD [H]

Synonym: BR0414

Alternate gene names: 23501317

Gene position: 420257-418974 (Counterclockwise)

Preceding gene: 23501319

Following gene: 23501316

Centisome position: 19.94

GC content: 61.21

Gene sequence:

>1284_bases
ATGAAAGTCCTGTTGATCGGTTCCGGTGGTCGCGAGCACGCCTTGGCATGGAAGCTTGCCGCCTCGCCCCTGCTTGAAAA
ACTATATTGCGCGCCCGGCAATCCCGGTATCGCCGCTGTAGCGGAGCTTGCCGATATCGGCGTGGACGACCATGCCGCGC
TGATTGCCTTTGCCAAGGAAAAGCATATCGACCTCGTGGTCGTGGGGCCGGAAGCGCCGCTTGTCACCGGGCTTGCCGAT
GAAATGCGCGCTGAGGGCATCCGCGTCTTCGGCCCGTCCAGGGCCGCAGCCCAGCTTGAAGGCTCGAAGGGCTTCACCAA
GGATCTCTGCGCGCGCTTCAATATCCCGACCGGCGCTTATGGCCGTTTCAACAATGCGCCAAAGGCGAAAGCCTATATCC
GCCAGCAGGGCGCGCCCATCGTGGTCAAGGCCGATGGGCTTGCCGCAGGCAAGGGCGTGGTTGTCGCCATGACGCTTCAG
GAAGCACTTGATGCGGTCGATTCCTGCTTTGAGGGCGCTTTCGGTGCGGCAGGCGCGGAAGTGGTGGTCGAGGAATTTCT
TGACGGTGAGGAAGCAAGCTTCTTCTGCATCTGCGATGGCAAGACGGCATTGCCGCTTGGCTCCGCGCAGGATCACAAGC
GCGTCGGCGATGGCGATACCGGCCCGAATACCGGCGGCATGGGCGCCTATGCGCCCGCCCCGGTGATGACACCGGAAATG
GTGGCGCGCACCATGCGCGAACTGATTGAACCCACCATGCGCGGCATGGCGGAAATCGGCGCACCCTTTTCCGGCATTCT
CTTTGCCGGGCTGATGATTACCAGCGATGGCCCGAAGCTCATCGAATATAATACCCGTTTCGGCGATCCGGAATGCCAGG
TATTGATGATGCGCCTCAACAGCGATCTGCTTGCCCTCATCAATGCTGCCGTTGACGGCAGACTTGATGAGGTTTCACTT
GAATGGAAAGACGAACCGGCGCTGACCGTAGTGATGGCCGCCGAAGGCTACCCGGCCAATGTGAAGAAGGGCAGCGTCAT
CCGCGACCTCGAAAAGCTGGAAAGCATTGATGGCGTAAAACTGTTCCATGCGGGCACGGCGCTGAAGGACGGCGCCATCG
TCGCCGGCGGCGGGCGCGTCCTCAACATCACCGCTACGGCGGCAACCGTAGCGCAAGCGCAGGCGCGTGCCTATGAGGCG
CTGAAGCTCATCGACTGGCCGGAAGGTTTTTACCGTTCTGATATCGGCTGGCGCGCCGTGGAGCGTGAGAAGGCTAATCG
CTGA

Upstream 100 bases:

>100_bases
CAAAATCTGACCCTTTGGTATTGCTCTCGACAAGCGGGAGCAATAGACAGCCGGAACAAGTCTTTATTGACTTACTGATC
TTAAGCGCAGGGAGCAAGGC

Downstream 100 bases:

>100_bases
TCGGTTTATGTTTCAGGTTACATGGACGCGGTTGCGTCCAGCCTTCTTGGCTTCGTAAAGCGCGCCATCGGCACGGCGTA
TCGTATCGTATCGTAAAGGC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKVLLIGSGGREHALAWKLAASPLLEKLYCAPGNPGIAAVAELADIGVDDHAALIAFAKEKHIDLVVVGPEAPLVTGLAD
EMRAEGIRVFGPSRAAAQLEGSKGFTKDLCARFNIPTGAYGRFNNAPKAKAYIRQQGAPIVVKADGLAAGKGVVVAMTLQ
EALDAVDSCFEGAFGAAGAEVVVEEFLDGEEASFFCICDGKTALPLGSAQDHKRVGDGDTGPNTGGMGAYAPAPVMTPEM
VARTMRELIEPTMRGMAEIGAPFSGILFAGLMITSDGPKLIEYNTRFGDPECQVLMMRLNSDLLALINAAVDGRLDEVSL
EWKDEPALTVVMAAEGYPANVKKGSVIRDLEKLESIDGVKLFHAGTALKDGAIVAGGGRVLNITATAATVAQAQARAYEA
LKLIDWPEGFYRSDIGWRAVEREKANR

Sequences:

>Translated_427_residues
MKVLLIGSGGREHALAWKLAASPLLEKLYCAPGNPGIAAVAELADIGVDDHAALIAFAKEKHIDLVVVGPEAPLVTGLAD
EMRAEGIRVFGPSRAAAQLEGSKGFTKDLCARFNIPTGAYGRFNNAPKAKAYIRQQGAPIVVKADGLAAGKGVVVAMTLQ
EALDAVDSCFEGAFGAAGAEVVVEEFLDGEEASFFCICDGKTALPLGSAQDHKRVGDGDTGPNTGGMGAYAPAPVMTPEM
VARTMRELIEPTMRGMAEIGAPFSGILFAGLMITSDGPKLIEYNTRFGDPECQVLMMRLNSDLLALINAAVDGRLDEVSL
EWKDEPALTVVMAAEGYPANVKKGSVIRDLEKLESIDGVKLFHAGTALKDGAIVAGGGRVLNITATAATVAQAQARAYEA
LKLIDWPEGFYRSDIGWRAVEREKANR
>Mature_427_residues
MKVLLIGSGGREHALAWKLAASPLLEKLYCAPGNPGIAAVAELADIGVDDHAALIAFAKEKHIDLVVVGPEAPLVTGLAD
EMRAEGIRVFGPSRAAAQLEGSKGFTKDLCARFNIPTGAYGRFNNAPKAKAYIRQQGAPIVVKADGLAAGKGVVVAMTLQ
EALDAVDSCFEGAFGAAGAEVVVEEFLDGEEASFFCICDGKTALPLGSAQDHKRVGDGDTGPNTGGMGAYAPAPVMTPEM
VARTMRELIEPTMRGMAEIGAPFSGILFAGLMITSDGPKLIEYNTRFGDPECQVLMMRLNSDLLALINAAVDGRLDEVSL
EWKDEPALTVVMAAEGYPANVKKGSVIRDLEKLESIDGVKLFHAGTALKDGAIVAGGGRVLNITATAATVAQAQARAYEA
LKLIDWPEGFYRSDIGWRAVEREKANR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=47.6415094339623, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=47.6415094339623, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=47.6415094339623, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=47.6415094339623, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=57.3426573426573, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=40.8983451536643, Blast_Score=292, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6321203, Length=448, Percent_Identity=41.7410714285714, Blast_Score=330, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=42.2273781902552, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=42.2273781902552, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44971; Mature: 44971

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLIGSGGREHALAWKLAASPLLEKLYCAPGNPGIAAVAELADIGVDDHAALIAFAKE
CEEEEEECCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECC
KHIDLVVVGPEAPLVTGLADEMRAEGIRVFGPSRAAAQLEGSKGFTKDLCARFNIPTGAY
CCEEEEEECCCCCEECCCHHHHHHCCEEEECCCCHHHEECCCCCCHHHHHHHCCCCCCCC
GRFNNAPKAKAYIRQQGAPIVVKADGLAAGKGVVVAMTLQEALDAVDSCFEGAFGAAGAE
CCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCHH
VVVEEFLDGEEASFFCICDGKTALPLGSAQDHKRVGDGDTGPNTGGMGAYAPAPVMTPEM
HHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VARTMRELIEPTMRGMAEIGAPFSGILFAGLMITSDGPKLIEYNTRFGDPECQVLMMRLN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEECCCCCEEEECCCCCCCHHEEEEEHHC
SDLLALINAAVDGRLDEVSLEWKDEPALTVVMAAEGYPANVKKGSVIRDLEKLESIDGVK
CHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEE
LFHAGTALKDGAIVAGGGRVLNITATAATVAQAQARAYEALKLIDWPEGFYRSDIGWRAV
EEECCCCCCCCEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHEEECCCCHHHCCCCHHHH
EREKANR
HHHHCCC
>Mature Secondary Structure
MKVLLIGSGGREHALAWKLAASPLLEKLYCAPGNPGIAAVAELADIGVDDHAALIAFAKE
CEEEEEECCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECC
KHIDLVVVGPEAPLVTGLADEMRAEGIRVFGPSRAAAQLEGSKGFTKDLCARFNIPTGAY
CCEEEEEECCCCCEECCCHHHHHHCCEEEECCCCHHHEECCCCCCHHHHHHHCCCCCCCC
GRFNNAPKAKAYIRQQGAPIVVKADGLAAGKGVVVAMTLQEALDAVDSCFEGAFGAAGAE
CCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCHH
VVVEEFLDGEEASFFCICDGKTALPLGSAQDHKRVGDGDTGPNTGGMGAYAPAPVMTPEM
HHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VARTMRELIEPTMRGMAEIGAPFSGILFAGLMITSDGPKLIEYNTRFGDPECQVLMMRLN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEECCCCCEEEECCCCCCCHHEEEEEHHC
SDLLALINAAVDGRLDEVSLEWKDEPALTVVMAAEGYPANVKKGSVIRDLEKLESIDGVK
CHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEE
LFHAGTALKDGAIVAGGGRVLNITATAATVAQAQARAYEALKLIDWPEGFYRSDIGWRAV
EEECCCCCCCCEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHEEECCCCHHHCCCCHHHH
EREKANR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]