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The map label for this gene is rnhB [H]

Identifier: 23501290

GI number: 23501290

Start: 394928

End: 395590

Strand: Reverse

Name: rnhB [H]

Synonym: BR0386

Alternate gene names: 23501290

Gene position: 395590-394928 (Counterclockwise)

Preceding gene: 23501291

Following gene: 23501281

Centisome position: 18.77

GC content: 62.44

Gene sequence:

>663_bases
ATGAAACGCTCGGCTTCTGATTCTCCGCTTCTCTTCGATCTTCCCCTCGCGCCTGATTTTTCACAAGAGCAGCAGCTCAT
GAAGCGCGGCCTGAAACATATTGCGGGCATTGACGAGGCTGGGCGCGGCCCCTTGGCCGGGCCGGTCGTCGCCGCGGCAG
TGGTGCTCGACCAGAACGATCTTCCCGAAGGGCTGGACGATTCCAAGCGGCTCACAGCCGCAAGGCGCGAAGCGCTTTAT
GAAATCATTCTGACCAAGGCGATCACGGTTTCGGTGGCAAGCCTCAGCGCCCGCAGTATCGACGCCAGCGATATCCGCAA
GGCGGCACTTGAGGCCATGCGCCGCGCCGTCATCGGCCTGACGCTCAAGCCATGCCATGCGCTGGTCGACGGGCGGGACG
TGCCTCCCGGCCTGCCCTGCCCCGGTTCGGCTCTGGTGAAAGGCGATCAGCGTTCCGTCTCGATTGCCGCCGCGTCTATC
GTGGCCAAGGTCACACGAGACCGCATGATGATCCGCGCTGGCGCCGCGCATCCGCCTTATGGGCTGGAAATCCATGCCGG
CTATGCCACCCAAAAACACCGCGCGGCCATTGAAAGCGAAGGGCCCGTTCCAGGTCTTCATCGCTATACATTCGCACCGA
TCAAAGGCCGCTTCGACTGTTAG

Upstream 100 bases:

>100_bases
CTGACTGTCCCTTCGGGCGGATAGGCACCCTGCTTTTACGCCACCATCCTCGGGCAGGGGCCTTGCGGAGAATTGGAAGC
AATGCGAGCATCGCCGCCAA

Downstream 100 bases:

>100_bases
AGCGGTTCCAGCGATTCCATTTTAACCGGAACCGCTCTAACTATTCGTTTTATCAGCTTATTCTAAAGCAAAACGCCGCC
CCGATAAGGCGGCGTTTTCC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKRSASDSPLLFDLPLAPDFSQEQQLMKRGLKHIAGIDEAGRGPLAGPVVAAAVVLDQNDLPEGLDDSKRLTAARREALY
EIILTKAITVSVASLSARSIDASDIRKAALEAMRRAVIGLTLKPCHALVDGRDVPPGLPCPGSALVKGDQRSVSIAAASI
VAKVTRDRMMIRAGAAHPPYGLEIHAGYATQKHRAAIESEGPVPGLHRYTFAPIKGRFDC

Sequences:

>Translated_220_residues
MKRSASDSPLLFDLPLAPDFSQEQQLMKRGLKHIAGIDEAGRGPLAGPVVAAAVVLDQNDLPEGLDDSKRLTAARREALY
EIILTKAITVSVASLSARSIDASDIRKAALEAMRRAVIGLTLKPCHALVDGRDVPPGLPCPGSALVKGDQRSVSIAAASI
VAKVTRDRMMIRAGAAHPPYGLEIHAGYATQKHRAAIESEGPVPGLHRYTFAPIKGRFDC
>Mature_220_residues
MKRSASDSPLLFDLPLAPDFSQEQQLMKRGLKHIAGIDEAGRGPLAGPVVAAAVVLDQNDLPEGLDDSKRLTAARREALY
EIILTKAITVSVASLSARSIDASDIRKAALEAMRRAVIGLTLKPCHALVDGRDVPPGLPCPGSALVKGDQRSVSIAAASI
VAKVTRDRMMIRAGAAHPPYGLEIHAGYATQKHRAAIESEGPVPGLHRYTFAPIKGRFDC

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=182, Percent_Identity=46.1538461538462, Blast_Score=141, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 23385; Mature: 23385

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSASDSPLLFDLPLAPDFSQEQQLMKRGLKHIAGIDEAGRGPLAGPVVAAAVVLDQND
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
LPEGLDDSKRLTAARREALYEIILTKAITVSVASLSARSIDASDIRKAALEAMRRAVIGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
TLKPCHALVDGRDVPPGLPCPGSALVKGDQRSVSIAAASIVAKVTRDRMMIRAGAAHPPY
CCHHHHHHHCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCC
GLEIHAGYATQKHRAAIESEGPVPGLHRYTFAPIKGRFDC
CEEEECCCCCHHHHHHHCCCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MKRSASDSPLLFDLPLAPDFSQEQQLMKRGLKHIAGIDEAGRGPLAGPVVAAAVVLDQND
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
LPEGLDDSKRLTAARREALYEIILTKAITVSVASLSARSIDASDIRKAALEAMRRAVIGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
TLKPCHALVDGRDVPPGLPCPGSALVKGDQRSVSIAAASIVAKVTRDRMMIRAGAAHPPY
CCHHHHHHHCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCC
GLEIHAGYATQKHRAAIESEGPVPGLHRYTFAPIKGRFDC
CEEEECCCCCHHHHHHHCCCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA