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The map label for this gene is fixI [H]

Identifier: 23501262

GI number: 23501262

Start: 368086

End: 370344

Strand: Reverse

Name: fixI [H]

Synonym: BR0357

Alternate gene names: 23501262

Gene position: 370344-368086 (Counterclockwise)

Preceding gene: 23501263

Following gene: 23501261

Centisome position: 17.57

GC content: 60.91

Gene sequence:

>2259_bases
ATGAGCTGCTGCGTCGAGAATGCACAGATAGCGACCGTCATTCAGTCCGTTTCGCCGGAAGAAATGCAGATCGCAAGTCG
TGCGCTCGGCGACGGGTTGCGCCAACTCGACCTTTCGGTGCCGGGGGTCCATTGCGGACTCTGCATCAAGACCATTGAGG
ATGCACTGGGAAAGATTTCCGGTGTTGCCTATGTGCGCGTCAACTTGACCGCGCGCCGCGTCACGCTGCGCTGGAAGGAT
GGCGAAACGCCGCCAAATGTCGTCGATACGCTGCGCAGGCTCGGCTATGAAGCACATATTTTCGACATGGCGCAGGACAA
GGATCCTACTTTCACGCGCCTTCTGATCGCGCTTGGCGTGGCAGCCTTCGCCTCCAGCAATGTCATGCTGCTGTCGGTTG
CCGTATGGTCGGGAGCGGATGCGGCAACGCGCGACATGTTCCATTGGATTTCCGGCCTCATCGCATTTCCGACGCTGGTT
TATTCGGGCCGCATTTTCTACGTTTCAGCGTGGAATGCGCTGCGCGCGGGGCGCGTGAACATGGATGTGCCGATTGCGCT
CGCCATTTCACTCGCCTTCGCCATGAGCGTCTATGAAACCATCCATCACGGCCAGCACGCCTATTTCGACGCTTCCGTAA
CGCTTCTCTTCTTCCTGTTGATCGGCCGCACACTCGACTACATGATGCGCGCCCGCGCCCGTTCCGCCGTGCGGGGCCTC
GCACAGCTTGGCAGCCGCGGCGCGGTGGTGATTGGCGAAAACGACGAGCGCAACTATCTGCCGGTCAATGAAATCCGCCC
CGGCATGCGCATCATTCTCGCGGCAGGCGAACGCGTACCCGTCGATGCCAAGGTGGAGGAAGGCCGCTCGGAACTTGATT
GTTCCATCGCATCGGGCGAAAGCGACGCCGTGCCCGTCGGCCCCGGCTCGGATATCCGTGCCGGCACGCTGAACCTTTCC
ACGCCGCTCGTCATCCGCGCCACGGCGGAAGCCAGGGATTCGTTCCTGTCGGAAATGGTGCGGCTTATGGATATCGCCGA
AGACGGACGCGCACACTATCGCCGCCTTGCCGACCGTGCCTCGGAACTTTACGTACCGATGGTGCACGCAACCGCTTTCC
TCGCCTTCGCAGGCTGGATGTTTTACACCGGCGGCGACTGGTACCGCTCGATCTATATTGCGATTTCGACCCTCATCATC
ACCTGCCCCTGCGCACTGGCGCTTGCGGTGCCCATCGTGCAGGTGGTGGCGGCGCGCCGCCTGTTTGAAAACGGCATCAT
GATCAAGGATGGTTCGGCCATGGAACGCATGGCCGAGATCGACACCGCCGTTTTCGACAAAACCGGCACACTGACGCTGG
GCCAACCATGGCTCATCAATGCCCAGGAAATCGAGCCGCATTATCTGGAAATCGCGGCAGAGCTTTCGCTCTATTCGCGC
CATCCATTATCGCGCGCGCTGGCGCTCTTTTCCGGTGCAACACCGATGACCCCCTTCACGCGCATCGAGGAAGTGCCGGG
CGCGGGCATCGAAGCCGAACTGGACGGCACCATCTATCGCCTCGGCAAGCAGGAATGGGCATGCGGCAAAGCCGAAACCG
CCCAGCCATCCGGCCCCGAAACCTGTCTTTCCATCGATGGCAGGCTCGTTCAGGTCTTCCGTTTCGAGGATCGCCCGCGT
GAAGACGCCGCCAATGCAATTGCGGCGCTAAAGCGCAACGGCCTCAAGCTCGGCATCATTTCCGGCGACAAGCAGGCGGC
GGTGAAGAAGCTTGCCGACTATCTTGGCGTCAACGATTATCACAGCGGCGTTCTGCCCGCCGACAAATCCAAACTGGTGC
AGGAGCTTTCCAGCGAAGGCCGAAAGGTCTTGATGGTGGGCGATGGCCTCAACGATGCGCCCGCGCTCGTTGCCGCGCAT
GTGTCCATGGCCCCCGCCACGGCCGCCGATATCGGCCGCAACGCCGCCGATTTCGTCTTCCTGCGTGAAAGCCTTTCTGC
CGTGCCGCTCGCCTTCGCCGTATCGAAGGAAGCGGGCAAGCTCGTCAGCCAGAATTTCGGTCTTTCCATCGGCTATAATA
TCATTGCCGTGCCGATTGCCGTCTTCGGCTATGTCACCCCGCTGGTGGCGGCCCTTTCCATGTCGCTTTCGTCCATCGTG
GTGGTGACAAACGCGTTGCGGCTTCGGGCAGGCAAACAGAAACAGCAAGCACGCCAGAGTGGCGCGCCTGCCACCACCAT
GAAGGAGGCGCATAAATGA

Upstream 100 bases:

>100_bases
TTGGCGAAGGTGCATGGATCATGGAAGTCAATGCCGATGCAGGGCTTGAAGACCCTTACCGGCACATCATCCGCGTTCTG
GTGAAGGACGGGAAAATCTT

Downstream 100 bases:

>100_bases
GCGGGCTTATCTTTCTCATTCCCATCGCGCTGTTTCTGGGGTTGCTCGGCCTTGCCGCATTCCTCTGGTCGCTGAAAAGC
GGCCAGTATGACGATCTGGA

Product: nitrogen fixation protein FixI

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MSCCVENAQIATVIQSVSPEEMQIASRALGDGLRQLDLSVPGVHCGLCIKTIEDALGKISGVAYVRVNLTARRVTLRWKD
GETPPNVVDTLRRLGYEAHIFDMAQDKDPTFTRLLIALGVAAFASSNVMLLSVAVWSGADAATRDMFHWISGLIAFPTLV
YSGRIFYVSAWNALRAGRVNMDVPIALAISLAFAMSVYETIHHGQHAYFDASVTLLFFLLIGRTLDYMMRARARSAVRGL
AQLGSRGAVVIGENDERNYLPVNEIRPGMRIILAAGERVPVDAKVEEGRSELDCSIASGESDAVPVGPGSDIRAGTLNLS
TPLVIRATAEARDSFLSEMVRLMDIAEDGRAHYRRLADRASELYVPMVHATAFLAFAGWMFYTGGDWYRSIYIAISTLII
TCPCALALAVPIVQVVAARRLFENGIMIKDGSAMERMAEIDTAVFDKTGTLTLGQPWLINAQEIEPHYLEIAAELSLYSR
HPLSRALALFSGATPMTPFTRIEEVPGAGIEAELDGTIYRLGKQEWACGKAETAQPSGPETCLSIDGRLVQVFRFEDRPR
EDAANAIAALKRNGLKLGIISGDKQAAVKKLADYLGVNDYHSGVLPADKSKLVQELSSEGRKVLMVGDGLNDAPALVAAH
VSMAPATAADIGRNAADFVFLRESLSAVPLAFAVSKEAGKLVSQNFGLSIGYNIIAVPIAVFGYVTPLVAALSMSLSSIV
VVTNALRLRAGKQKQQARQSGAPATTMKEAHK

Sequences:

>Translated_752_residues
MSCCVENAQIATVIQSVSPEEMQIASRALGDGLRQLDLSVPGVHCGLCIKTIEDALGKISGVAYVRVNLTARRVTLRWKD
GETPPNVVDTLRRLGYEAHIFDMAQDKDPTFTRLLIALGVAAFASSNVMLLSVAVWSGADAATRDMFHWISGLIAFPTLV
YSGRIFYVSAWNALRAGRVNMDVPIALAISLAFAMSVYETIHHGQHAYFDASVTLLFFLLIGRTLDYMMRARARSAVRGL
AQLGSRGAVVIGENDERNYLPVNEIRPGMRIILAAGERVPVDAKVEEGRSELDCSIASGESDAVPVGPGSDIRAGTLNLS
TPLVIRATAEARDSFLSEMVRLMDIAEDGRAHYRRLADRASELYVPMVHATAFLAFAGWMFYTGGDWYRSIYIAISTLII
TCPCALALAVPIVQVVAARRLFENGIMIKDGSAMERMAEIDTAVFDKTGTLTLGQPWLINAQEIEPHYLEIAAELSLYSR
HPLSRALALFSGATPMTPFTRIEEVPGAGIEAELDGTIYRLGKQEWACGKAETAQPSGPETCLSIDGRLVQVFRFEDRPR
EDAANAIAALKRNGLKLGIISGDKQAAVKKLADYLGVNDYHSGVLPADKSKLVQELSSEGRKVLMVGDGLNDAPALVAAH
VSMAPATAADIGRNAADFVFLRESLSAVPLAFAVSKEAGKLVSQNFGLSIGYNIIAVPIAVFGYVTPLVAALSMSLSSIV
VVTNALRLRAGKQKQQARQSGAPATTMKEAHK
>Mature_751_residues
SCCVENAQIATVIQSVSPEEMQIASRALGDGLRQLDLSVPGVHCGLCIKTIEDALGKISGVAYVRVNLTARRVTLRWKDG
ETPPNVVDTLRRLGYEAHIFDMAQDKDPTFTRLLIALGVAAFASSNVMLLSVAVWSGADAATRDMFHWISGLIAFPTLVY
SGRIFYVSAWNALRAGRVNMDVPIALAISLAFAMSVYETIHHGQHAYFDASVTLLFFLLIGRTLDYMMRARARSAVRGLA
QLGSRGAVVIGENDERNYLPVNEIRPGMRIILAAGERVPVDAKVEEGRSELDCSIASGESDAVPVGPGSDIRAGTLNLST
PLVIRATAEARDSFLSEMVRLMDIAEDGRAHYRRLADRASELYVPMVHATAFLAFAGWMFYTGGDWYRSIYIAISTLIIT
CPCALALAVPIVQVVAARRLFENGIMIKDGSAMERMAEIDTAVFDKTGTLTLGQPWLINAQEIEPHYLEIAAELSLYSRH
PLSRALALFSGATPMTPFTRIEEVPGAGIEAELDGTIYRLGKQEWACGKAETAQPSGPETCLSIDGRLVQVFRFEDRPRE
DAANAIAALKRNGLKLGIISGDKQAAVKKLADYLGVNDYHSGVLPADKSKLVQELSSEGRKVLMVGDGLNDAPALVAAHV
SMAPATAADIGRNAADFVFLRESLSAVPLAFAVSKEAGKLVSQNFGLSIGYNIIAVPIAVFGYVTPLVAALSMSLSSIVV
VTNALRLRAGKQKQQARQSGAPATTMKEAHK

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=812, Percent_Identity=26.9704433497537, Blast_Score=266, Evalue=7e-71,
Organism=Homo sapiens, GI115529486, Length=825, Percent_Identity=25.2121212121212, Blast_Score=193, Evalue=4e-49,
Organism=Homo sapiens, GI55743073, Length=729, Percent_Identity=25.9259259259259, Blast_Score=191, Evalue=3e-48,
Organism=Escherichia coli, GI1786691, Length=760, Percent_Identity=32.1052631578947, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI1789879, Length=703, Percent_Identity=28.1650071123755, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1786914, Length=439, Percent_Identity=25.7403189066059, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI2367363, Length=294, Percent_Identity=25.5102040816327, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556548, Length=656, Percent_Identity=25.7621951219512, Blast_Score=169, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6320475, Length=652, Percent_Identity=27.760736196319, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6319772, Length=608, Percent_Identity=26.3157894736842, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6325221, Length=150, Percent_Identity=31.3333333333333, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321430, Length=150, Percent_Identity=30.6666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221329854, Length=550, Percent_Identity=25.8181818181818, Blast_Score=170, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR000695
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 81100; Mature: 80969

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCCVENAQIATVIQSVSPEEMQIASRALGDGLRQLDLSVPGVHCGLCIKTIEDALGKIS
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
GVAYVRVNLTARRVTLRWKDGETPPNVVDTLRRLGYEAHIFDMAQDKDPTFTRLLIALGV
CEEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
AAFASSNVMLLSVAVWSGADAATRDMFHWISGLIAFPTLVYSGRIFYVSAWNALRAGRVN
HHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHCCCCC
MDVPIALAISLAFAMSVYETIHHGQHAYFDASVTLLFFLLIGRTLDYMMRARARSAVRGL
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLGSRGAVVIGENDERNYLPVNEIRPGMRIILAAGERVPVDAKVEEGRSELDCSIASGE
HHHCCCCEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCHHHCCCCCCCEEEECCC
SDAVPVGPGSDIRAGTLNLSTPLVIRATAEARDSFLSEMVRLMDIAEDGRAHYRRLADRA
CCCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SELYVPMVHATAFLAFAGWMFYTGGDWYRSIYIAISTLIITCPCALALAVPIVQVVAARR
HHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFENGIMIKDGSAMERMAEIDTAVFDKTGTLTLGQPWLINAQEIEPHYLEIAAELSLYSR
HHHCCCEEECCHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHCCCHHHHHHHHHHHHHC
HPLSRALALFSGATPMTPFTRIEEVPGAGIEAELDGTIYRLGKQEWACGKAETAQPSGPE
CHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEECCCHHEECCCCCCCCCCCCCCCCCCCC
TCLSIDGRLVQVFRFEDRPREDAANAIAALKRNGLKLGIISGDKQAAVKKLADYLGVNDY
CEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCC
HSGVLPADKSKLVQELSSEGRKVLMVGDGLNDAPALVAAHVSMAPATAADIGRNAADFVF
CCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHCCCHHHHHH
LRESLSAVPLAFAVSKEAGKLVSQNFGLSIGYNIIAVPIAVFGYVTPLVAALSMSLSSIV
HHHHHHHCHHHEEHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVTNALRLRAGKQKQQARQSGAPATTMKEAHK
HHHHHHHHHCCCHHHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
SCCVENAQIATVIQSVSPEEMQIASRALGDGLRQLDLSVPGVHCGLCIKTIEDALGKIS
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
GVAYVRVNLTARRVTLRWKDGETPPNVVDTLRRLGYEAHIFDMAQDKDPTFTRLLIALGV
CEEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
AAFASSNVMLLSVAVWSGADAATRDMFHWISGLIAFPTLVYSGRIFYVSAWNALRAGRVN
HHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHCCCCC
MDVPIALAISLAFAMSVYETIHHGQHAYFDASVTLLFFLLIGRTLDYMMRARARSAVRGL
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLGSRGAVVIGENDERNYLPVNEIRPGMRIILAAGERVPVDAKVEEGRSELDCSIASGE
HHHCCCCEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCHHHCCCCCCCEEEECCC
SDAVPVGPGSDIRAGTLNLSTPLVIRATAEARDSFLSEMVRLMDIAEDGRAHYRRLADRA
CCCCCCCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SELYVPMVHATAFLAFAGWMFYTGGDWYRSIYIAISTLIITCPCALALAVPIVQVVAARR
HHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFENGIMIKDGSAMERMAEIDTAVFDKTGTLTLGQPWLINAQEIEPHYLEIAAELSLYSR
HHHCCCEEECCHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHCCCHHHHHHHHHHHHHC
HPLSRALALFSGATPMTPFTRIEEVPGAGIEAELDGTIYRLGKQEWACGKAETAQPSGPE
CHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEECCCHHEECCCCCCCCCCCCCCCCCCCC
TCLSIDGRLVQVFRFEDRPREDAANAIAALKRNGLKLGIISGDKQAAVKKLADYLGVNDY
CEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCC
HSGVLPADKSKLVQELSSEGRKVLMVGDGLNDAPALVAAHVSMAPATAADIGRNAADFVF
CCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHCCCHHHHHH
LRESLSAVPLAFAVSKEAGKLVSQNFGLSIGYNIIAVPIAVFGYVTPLVAALSMSLSSIV
HHHHHHHCHHHEEHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVTNALRLRAGKQKQQARQSGAPATTMKEAHK
HHHHHHHHHCCCHHHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2536685; 11481432 [H]