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The map label for this gene is sppA [H]

Identifier: 23501065

GI number: 23501065

Start: 170483

End: 171478

Strand: Reverse

Name: sppA [H]

Synonym: BR0154

Alternate gene names: 23501065

Gene position: 171478-170483 (Counterclockwise)

Preceding gene: 23501070

Following gene: 23501064

Centisome position: 8.14

GC content: 59.94

Gene sequence:

>996_bases
ATGGACGAACGAATGGCTCATGACGCCGAAGCAATGATCGAAAGACGCAGGCTGCGCCGGAAGCTGACCAGCTGGCGATT
GGCTTTCCTCGTGCTTCTGGCCGCGCTGATAGCCGGGTTCGCCTTTTATTCGCTGCGCAGCGCCAAGTTTAACCAGCCGC
ATATCGCCAAGGTGCGCATCGAGGGCACGATCTTCGAGAACGAGGAGCTTTTGAAGCGCCTCGACAAAATCGCCGGGGAC
GACGCCGTGAAGGGTGTCATCCTGCTGCTCGATTCGCCGGGGGGCACCACCGTCGGCGGTGAAGCGATCTACGATGCCGT
GCGCAAGATCGCCACAAAGAAACCGGTCGTAACCCAGGTCGGCACGCTGGCCGCCTCCGCGGGCTATATGATCGCCAGCG
CATCCGATCATATCGTGGCGCGGCAAACCTCCATTGTCGGCTCCATCGGCGTTCTCTTCCAGTATCCCGACCTTTCCAAA
CTGCTCGATACGCTCGGCGTGAAGGTCGAAACCATCAAGTCCTCACCGCTGAAGGCCGAGCCCAATTATTTCAGCCCGGC
GAGCGAGGAAGCCAAGAACATGATCCGCAGCATGATCATGGATTCCTATGACTGGTTCGTCGGCATCGTGCAGGAACGCC
GCGCCTTCACCCATGAACAGGCGCTGGCCCTTGCCAATGGCGCGGTGTTCACCGGGCGGCAGGCACTCGACAAAAAACTG
ATCGATGGCCTTGGCGGCGAGGCGGAAGCCGTCGCATGGCTTCAGACCAAGGGACTGTCGGACAAGCTGCCTCGCCTTGA
ATGGAAGCCGGTCGGCAGCGAAACAGGTTTTTCGCTGCGTGATCTCATCATCCATGCCGGCGCGCGCCTTCTGGGCCTAC
CACAGGAAGCCGATGGCGCCATCAAGGAAATCGCCAGGGATCGCATCTTCCTTGACGGACTCCTTTCCGTTTGGCACGTT
GATGGAATAGCCGGTGCAAACCCGGCAAACCAGTAG

Upstream 100 bases:

>100_bases
GAAGAATTCCCTTAAGGTTGAGAACAGGCTTAGAATCCACCGCCTTGGAGGCAGAAAGCGGAGAGTCAATTTTTCGCGAT
CAAGCCCTTATATTACAGAT

Downstream 100 bases:

>100_bases
AGTCCTTCCGGCAGGCAGGGCAGAATTGAAACAGGGGTCTAAAGCCGTGATCAAATCGGAACTTGTTCAGATTATCGCAA
GCCGCAATCCACATCTTTTC

Product: signal peptide peptidase SppA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MDERMAHDAEAMIERRRLRRKLTSWRLAFLVLLAALIAGFAFYSLRSAKFNQPHIAKVRIEGTIFENEELLKRLDKIAGD
DAVKGVILLLDSPGGTTVGGEAIYDAVRKIATKKPVVTQVGTLAASAGYMIASASDHIVARQTSIVGSIGVLFQYPDLSK
LLDTLGVKVETIKSSPLKAEPNYFSPASEEAKNMIRSMIMDSYDWFVGIVQERRAFTHEQALALANGAVFTGRQALDKKL
IDGLGGEAEAVAWLQTKGLSDKLPRLEWKPVGSETGFSLRDLIIHAGARLLGLPQEADGAIKEIARDRIFLDGLLSVWHV
DGIAGANPANQ

Sequences:

>Translated_331_residues
MDERMAHDAEAMIERRRLRRKLTSWRLAFLVLLAALIAGFAFYSLRSAKFNQPHIAKVRIEGTIFENEELLKRLDKIAGD
DAVKGVILLLDSPGGTTVGGEAIYDAVRKIATKKPVVTQVGTLAASAGYMIASASDHIVARQTSIVGSIGVLFQYPDLSK
LLDTLGVKVETIKSSPLKAEPNYFSPASEEAKNMIRSMIMDSYDWFVGIVQERRAFTHEQALALANGAVFTGRQALDKKL
IDGLGGEAEAVAWLQTKGLSDKLPRLEWKPVGSETGFSLRDLIIHAGARLLGLPQEADGAIKEIARDRIFLDGLLSVWHV
DGIAGANPANQ
>Mature_331_residues
MDERMAHDAEAMIERRRLRRKLTSWRLAFLVLLAALIAGFAFYSLRSAKFNQPHIAKVRIEGTIFENEELLKRLDKIAGD
DAVKGVILLLDSPGGTTVGGEAIYDAVRKIATKKPVVTQVGTLAASAGYMIASASDHIVARQTSIVGSIGVLFQYPDLSK
LLDTLGVKVETIKSSPLKAEPNYFSPASEEAKNMIRSMIMDSYDWFVGIVQERRAFTHEQALALANGAVFTGRQALDKKL
IDGLGGEAEAVAWLQTKGLSDKLPRLEWKPVGSETGFSLRDLIIHAGARLLGLPQEADGAIKEIARDRIFLDGLLSVWHV
DGIAGANPANQ

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=220, Percent_Identity=31.8181818181818, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787527, Length=192, Percent_Identity=29.1666666666667, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 36078; Mature: 36078

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERMAHDAEAMIERRRLRRKLTSWRLAFLVLLAALIAGFAFYSLRSAKFNQPHIAKVRI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
EGTIFENEELLKRLDKIAGDDAVKGVILLLDSPGGTTVGGEAIYDAVRKIATKKPVVTQV
EEEEECCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHH
GTLAASAGYMIASASDHIVARQTSIVGSIGVLFQYPDLSKLLDTLGVKVETIKSSPLKAE
HHHHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHCCEEEEECCCCCCCC
PNYFSPASEEAKNMIRSMIMDSYDWFVGIVQERRAFTHEQALALANGAVFTGRQALDKKL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHH
IDGLGGEAEAVAWLQTKGLSDKLPRLEWKPVGSETGFSLRDLIIHAGARLLGLPQEADGA
HHCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCHHHCCCCCCCCHH
IKEIARDRIFLDGLLSVWHVDGIAGANPANQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDERMAHDAEAMIERRRLRRKLTSWRLAFLVLLAALIAGFAFYSLRSAKFNQPHIAKVRI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
EGTIFENEELLKRLDKIAGDDAVKGVILLLDSPGGTTVGGEAIYDAVRKIATKKPVVTQV
EEEEECCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHH
GTLAASAGYMIASASDHIVARQTSIVGSIGVLFQYPDLSKLLDTLGVKVETIKSSPLKAE
HHHHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHCCEEEEECCCCCCCC
PNYFSPASEEAKNMIRSMIMDSYDWFVGIVQERRAFTHEQALALANGAVFTGRQALDKKL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHH
IDGLGGEAEAVAWLQTKGLSDKLPRLEWKPVGSETGFSLRDLIIHAGARLLGLPQEADGA
HHCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCHHHCCCCCCCCHH
IKEIARDRIFLDGLLSVWHVDGIAGANPANQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]