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The map label for this gene is engD [H]

Identifier: 23100935

GI number: 23100935

Start: 3614438

End: 3615538

Strand: Reverse

Name: engD [H]

Synonym: OB3480

Alternate gene names: 23100935

Gene position: 3615538-3614438 (Counterclockwise)

Preceding gene: 23100936

Following gene: 23100934

Centisome position: 99.59

GC content: 38.33

Gene sequence:

>1101_bases
ATGGCATTGACAGCAGGAATCGTAGGTCTTCCGAATGTTGGGAAATCAACGCTTTTTAATGCGATTACACAAGCTGGAGC
AGAAGCTGCAAATTACCCATTTGCAACGATTGATCCAAATGTAGGGATCGTGGAAGTACCTGACGAACGATTAAATAAAT
TAACCGAACTTGTAAATCCAAAGAAAACAGTACCTACTGCATTTGAATTTACAGATATTGCAGGAATTGTAAAAGGCGCT
AGTAAAGGAGAAGGTCTTGGAAACCAGTTCTTATCTCATATACGTCAAGTAGATGCAATCTGTCAGGTTGTACGTTGCTT
TGAAGATGAGAATATTACGCATGTTTCTGGTAAGGTGGACCCGATTGATGATATCGAAATTATTAATTTGGAATTAATTT
TAGCAGATTTAGATACCGTTAATAAGCGATATCAGCGTGTAGAGAAGCTAGCTCGACAAAAAGATAAAGACGCTGTAGCA
GAGCAAGCAGTCTTAGCTATAGTGAAAGAGGGCTTAGAAAATGATAAGCCTGTTCGTGCATTAGAGTTCTCAGAAGATCA
ATGGAAGATTGTGAAAGGTTTGCATTTACTTACTAGTAAGCCAACATTATATGTTGCAAATGTAAGTGAAGACGAAGTCG
CAGATCCTGATGGGAATGAATATGTACAGAAAGTAAAAGCGCATGCTGCAGATGAAAATGCAGAAGTAATCATTGTTTGC
GCTCGTATCGAAGAAGAAATTGCAGAATTAGAAGCGGAAGAAAAAGAAGAATTCCTAGAAGATTTAGGAATTGCGGAATC
TGGTTTAGATCAACTTATTAAAGCATCTTATAACTTACTTGGACTAGCTACGTATTTTACTGCTGGAGAACAAGAAGTAC
GTGCCTGGACATTCCGTAAAGGGATTAAAGCACCACAAGCAGCAGGAATTATTCATACAGACTTTGAAAGAGGATTTATT
CGTGCAGAGACCGTTTCCTATACAGATCTTGTGGAAGCTGAAAACATGACAACGGCAAGAGAACGTGGAAAAGTTAGACT
TGAAGGAAAAGAGTACATCGTAAAAGATGGCGATGTTATTCATTTCCGATTCAACGTATAA

Upstream 100 bases:

>100_bases
TTTGTTTTTTACTTGCTTGTTTTTTTTATGTCTAATATCTATAATTGGATTAATGAAAGCCTTCATATGAATGGAATCCT
TAAGGAGTGGAAAAAGAAAT

Downstream 100 bases:

>100_bases
TATTTAATTAACCTAGAAATTCCCATTTAAAATAGCGTAGATTAGTTTTTCGAGCACTTGTCAAGGAATACATTATTTGA
TATAATACTACATTGTGAGT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MALTAGIVGLPNVGKSTLFNAITQAGAEAANYPFATIDPNVGIVEVPDERLNKLTELVNPKKTVPTAFEFTDIAGIVKGA
SKGEGLGNQFLSHIRQVDAICQVVRCFEDENITHVSGKVDPIDDIEIINLELILADLDTVNKRYQRVEKLARQKDKDAVA
EQAVLAIVKEGLENDKPVRALEFSEDQWKIVKGLHLLTSKPTLYVANVSEDEVADPDGNEYVQKVKAHAADENAEVIIVC
ARIEEEIAELEAEEKEEFLEDLGIAESGLDQLIKASYNLLGLATYFTAGEQEVRAWTFRKGIKAPQAAGIIHTDFERGFI
RAETVSYTDLVEAENMTTARERGKVRLEGKEYIVKDGDVIHFRFNV

Sequences:

>Translated_366_residues
MALTAGIVGLPNVGKSTLFNAITQAGAEAANYPFATIDPNVGIVEVPDERLNKLTELVNPKKTVPTAFEFTDIAGIVKGA
SKGEGLGNQFLSHIRQVDAICQVVRCFEDENITHVSGKVDPIDDIEIINLELILADLDTVNKRYQRVEKLARQKDKDAVA
EQAVLAIVKEGLENDKPVRALEFSEDQWKIVKGLHLLTSKPTLYVANVSEDEVADPDGNEYVQKVKAHAADENAEVIIVC
ARIEEEIAELEAEEKEEFLEDLGIAESGLDQLIKASYNLLGLATYFTAGEQEVRAWTFRKGIKAPQAAGIIHTDFERGFI
RAETVSYTDLVEAENMTTARERGKVRLEGKEYIVKDGDVIHFRFNV
>Mature_365_residues
ALTAGIVGLPNVGKSTLFNAITQAGAEAANYPFATIDPNVGIVEVPDERLNKLTELVNPKKTVPTAFEFTDIAGIVKGAS
KGEGLGNQFLSHIRQVDAICQVVRCFEDENITHVSGKVDPIDDIEIINLELILADLDTVNKRYQRVEKLARQKDKDAVAE
QAVLAIVKEGLENDKPVRALEFSEDQWKIVKGLHLLTSKPTLYVANVSEDEVADPDGNEYVQKVKAHAADENAEVIIVCA
RIEEEIAELEAEEKEEFLEDLGIAESGLDQLIKASYNLLGLATYFTAGEQEVRAWTFRKGIKAPQAAGIIHTDFERGFIR
AETVSYTDLVEAENMTTARERGKVRLEGKEYIVKDGDVIHFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=370, Percent_Identity=46.7567567567568, Blast_Score=307, Evalue=9e-84,
Organism=Homo sapiens, GI58761502, Length=226, Percent_Identity=42.4778761061947, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI24308117, Length=111, Percent_Identity=36.9369369369369, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787454, Length=366, Percent_Identity=57.9234972677596, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1789574, Length=138, Percent_Identity=30.4347826086957, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17509631, Length=366, Percent_Identity=43.9890710382514, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17508313, Length=104, Percent_Identity=34.6153846153846, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17552324, Length=134, Percent_Identity=31.3432835820896, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319499, Length=372, Percent_Identity=41.9354838709677, Blast_Score=266, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6321773, Length=381, Percent_Identity=37.007874015748, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6321649, Length=287, Percent_Identity=30.3135888501742, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321962, Length=168, Percent_Identity=25.5952380952381, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640873, Length=369, Percent_Identity=42.8184281842818, Blast_Score=277, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24640877, Length=369, Percent_Identity=42.8184281842818, Blast_Score=277, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24640875, Length=369, Percent_Identity=42.8184281842818, Blast_Score=277, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24640879, Length=328, Percent_Identity=40.5487804878049, Blast_Score=224, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24585318, Length=137, Percent_Identity=30.6569343065693, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40424; Mature: 40293

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTAGIVGLPNVGKSTLFNAITQAGAEAANYPFATIDPNVGIVEVPDERLNKLTELVNP
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCEEECCHHHHHHHHHHCCC
KKTVPTAFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICQVVRCFEDENITHVSGKVD
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PIDDIEIINLELILADLDTVNKRYQRVEKLARQKDKDAVAEQAVLAIVKEGLENDKPVRA
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LEFSEDQWKIVKGLHLLTSKPTLYVANVSEDEVADPDGNEYVQKVKAHAADENAEVIIVC
EECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEE
ARIEEEIAELEAEEKEEFLEDLGIAESGLDQLIKASYNLLGLATYFTAGEQEVRAWTFRK
HHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GIKAPQAAGIIHTDFERGFIRAETVSYTDLVEAENMTTARERGKVRLEGKEYIVKDGDVI
CCCCCCCCCCEEECCCCCEEEEECCCHHHHHHHCCCHHHHHCCCEEECCCEEEEECCCEE
HFRFNV
EEEECC
>Mature Secondary Structure 
ALTAGIVGLPNVGKSTLFNAITQAGAEAANYPFATIDPNVGIVEVPDERLNKLTELVNP
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCEEECCHHHHHHHHHHCCC
KKTVPTAFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICQVVRCFEDENITHVSGKVD
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PIDDIEIINLELILADLDTVNKRYQRVEKLARQKDKDAVAEQAVLAIVKEGLENDKPVRA
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LEFSEDQWKIVKGLHLLTSKPTLYVANVSEDEVADPDGNEYVQKVKAHAADENAEVIIVC
EECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEE
ARIEEEIAELEAEEKEEFLEDLGIAESGLDQLIKASYNLLGLATYFTAGEQEVRAWTFRK
HHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GIKAPQAAGIIHTDFERGFIRAETVSYTDLVEAENMTTARERGKVRLEGKEYIVKDGDVI
CCCCCCCCCCEEECCCCCEEEEECCCHHHHHHHCCCHHHHHCCCEEECCCEEEEECCCEE
HFRFNV
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]