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The map label for this gene is purR [H]

Identifier: 23100696

GI number: 23100696

Start: 3379139

End: 3380170

Strand: Direct

Name: purR [H]

Synonym: OB3241

Alternate gene names: 23100696

Gene position: 3379139-3380170 (Clockwise)

Preceding gene: 23100691

Following gene: 23100698

Centisome position: 93.08

GC content: 37.79

Gene sequence:

>1032_bases
ATGAATGGATTAGGTGAAATAATGAGTAATAAACAATCCCGTCGAGTGACCTTAATGGAAGTGGCTAACCACGCTGGTGT
ATCAAGAGCAACAGCATCATTGGTCGCACGTAATAGCACAAAAATCTCTAACGATACAAAAGAAAAAGTATTGCAATCCA
TGAAAGAACTAGGATACGTATATGATAGAATTGCTGCTAACTTACGATCTCAAACTTCCACAACAATCGGTATTATTATT
ACAGATGTAGCCAACACTTTCTTTTCAGAATTGCTGTTAGGTGTACACCAAGAATTAGAGAAATTTGGATACACTGTATT
TTTAGGAACAACGTTTGACTCCGTCGATAATCAGGTGAAGCTAATCTCCACCATGCTAGAACACCGTGTCGGTGGAATTA
TATTATGTCCTGTCTCCAGCACTTCAACAGAAACGATTGAACGTGTGCAAAATATTGATATACCTATGGTTGTCGCTGTC
AGAGAACTTCCTTTTATCGAAACCAACTATGTTGGTCTTAACTATACACAAGGTGCAAAAAAAGCTACCAGCTATCTATT
AGAAAAAGGACATACTCGAATTGCATTCTTAGGTGGCCGCTCAGAGTCTTCTACATGGCACGAAAGAATAGAAGGCTTTT
ATCAAGCACATCAGAACGCCGGGATAAATGTTGACTCATCATTAATTGTTGATAGTTCTCCAACAAGAGAAGGCGGTATA
GAAGCCGTTCAAACAATAATTAATCATCCAAACCGTCCAACAGCCATTTTTTGCTTTAGTGATATGATCGCTTTTGGTGT
AATAATTGGCTTACGAAAAGCTGGAATTACACCTGGTGAAGACATTGAAATTGTTGGATTTGATAATGTTCCTGAGACAG
AAATTGTTTATCCTCCATTAACAACCGTTTCTTCCTTTCCTCAACAAATTGGAGCAGAGGCGGCTAACCTATTGCATAAA
AAAATGACATCAGATACAAACGAAAAGGAACGTATTATTCTCCAACCTGAATTGGTAAAACGAGATAAATAA

Upstream 100 bases:

>100_bases
GCTCCTCATTTCCTGCTTTTATTATATTGCTTTTTAAATATCAAAAATGTAAGATAAACGTATCGCAATGAATCAATTAC
TGTAGAGTAATGCGGATAAT

Downstream 100 bases:

>100_bases
TACATGTAAGGTAGTAAAATCTTAACTTGGATTAATTAGAAGAGTATGATTTTACTACCTTTTTTCATAATGGTTGTATC
GATTTCAACTGCTATTTTCA

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNGLGEIMSNKQSRRVTLMEVANHAGVSRATASLVARNSTKISNDTKEKVLQSMKELGYVYDRIAANLRSQTSTTIGIII
TDVANTFFSELLLGVHQELEKFGYTVFLGTTFDSVDNQVKLISTMLEHRVGGIILCPVSSTSTETIERVQNIDIPMVVAV
RELPFIETNYVGLNYTQGAKKATSYLLEKGHTRIAFLGGRSESSTWHERIEGFYQAHQNAGINVDSSLIVDSSPTREGGI
EAVQTIINHPNRPTAIFCFSDMIAFGVIIGLRKAGITPGEDIEIVGFDNVPETEIVYPPLTTVSSFPQQIGAEAANLLHK
KMTSDTNEKERIILQPELVKRDK

Sequences:

>Translated_343_residues
MNGLGEIMSNKQSRRVTLMEVANHAGVSRATASLVARNSTKISNDTKEKVLQSMKELGYVYDRIAANLRSQTSTTIGIII
TDVANTFFSELLLGVHQELEKFGYTVFLGTTFDSVDNQVKLISTMLEHRVGGIILCPVSSTSTETIERVQNIDIPMVVAV
RELPFIETNYVGLNYTQGAKKATSYLLEKGHTRIAFLGGRSESSTWHERIEGFYQAHQNAGINVDSSLIVDSSPTREGGI
EAVQTIINHPNRPTAIFCFSDMIAFGVIIGLRKAGITPGEDIEIVGFDNVPETEIVYPPLTTVSSFPQQIGAEAANLLHK
KMTSDTNEKERIILQPELVKRDK
>Mature_343_residues
MNGLGEIMSNKQSRRVTLMEVANHAGVSRATASLVARNSTKISNDTKEKVLQSMKELGYVYDRIAANLRSQTSTTIGIII
TDVANTFFSELLLGVHQELEKFGYTVFLGTTFDSVDNQVKLISTMLEHRVGGIILCPVSSTSTETIERVQNIDIPMVVAV
RELPFIETNYVGLNYTQGAKKATSYLLEKGHTRIAFLGGRSESSTWHERIEGFYQAHQNAGINVDSSLIVDSSPTREGGI
EAVQTIINHPNRPTAIFCFSDMIAFGVIIGLRKAGITPGEDIEIVGFDNVPETEIVYPPLTTVSSFPQQIGAEAANLLHK
KMTSDTNEKERIILQPELVKRDK

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=314, Percent_Identity=33.1210191082803, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1790194, Length=326, Percent_Identity=31.2883435582822, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787948, Length=328, Percent_Identity=30.4878048780488, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787906, Length=336, Percent_Identity=28.2738095238095, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=30.1829268292683, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1789202, Length=288, Percent_Identity=30.9027777777778, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789068, Length=289, Percent_Identity=28.719723183391, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI48994940, Length=316, Percent_Identity=25.6329113924051, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786540, Length=331, Percent_Identity=25.6797583081571, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787580, Length=311, Percent_Identity=26.6881028938907, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790715, Length=328, Percent_Identity=24.390243902439, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786268, Length=294, Percent_Identity=26.530612244898, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1790192, Length=276, Percent_Identity=26.4492753623188, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789456, Length=317, Percent_Identity=22.397476340694, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790689, Length=328, Percent_Identity=24.0853658536585, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37764; Mature: 37764

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLGEIMSNKQSRRVTLMEVANHAGVSRATASLVARNSTKISNDTKEKVLQSMKELGYV
CCCHHHHHCCCHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
YDRIAANLRSQTSTTIGIIITDVANTFFSELLLGVHQELEKFGYTVFLGTTFDSVDNQVK
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
LISTMLEHRVGGIILCPVSSTSTETIERVQNIDIPMVVAVRELPFIETNYVGLNYTQGAK
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCEEEHHHHCCCEEECCCCCCCCCCHH
KATSYLLEKGHTRIAFLGGRSESSTWHERIEGFYQAHQNAGINVDSSLIVDSSPTREGGI
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHH
EAVQTIINHPNRPTAIFCFSDMIAFGVIIGLRKAGITPGEDIEIVGFDNVPETEIVYPPL
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEECCCH
TTVSSFPQQIGAEAANLLHKKMTSDTNEKERIILQPELVKRDK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCC
>Mature Secondary Structure
MNGLGEIMSNKQSRRVTLMEVANHAGVSRATASLVARNSTKISNDTKEKVLQSMKELGYV
CCCHHHHHCCCHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
YDRIAANLRSQTSTTIGIIITDVANTFFSELLLGVHQELEKFGYTVFLGTTFDSVDNQVK
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
LISTMLEHRVGGIILCPVSSTSTETIERVQNIDIPMVVAVRELPFIETNYVGLNYTQGAK
HHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCEEEHHHHCCCEEECCCCCCCCCCHH
KATSYLLEKGHTRIAFLGGRSESSTWHERIEGFYQAHQNAGINVDSSLIVDSSPTREGGI
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHH
EAVQTIINHPNRPTAIFCFSDMIAFGVIIGLRKAGITPGEDIEIVGFDNVPETEIVYPPL
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEECCCH
TTVSSFPQQIGAEAANLLHKKMTSDTNEKERIILQPELVKRDK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA