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The map label for this gene is yngG [H]

Identifier: 23100685

GI number: 23100685

Start: 3365510

End: 3366466

Strand: Reverse

Name: yngG [H]

Synonym: OB3230

Alternate gene names: 23100685

Gene position: 3366466-3365510 (Counterclockwise)

Preceding gene: 23100686

Following gene: 23100682

Centisome position: 92.73

GC content: 39.71

Gene sequence:

>957_bases
ATGGCAGATCAAGCGTTTTTACTTCCTACAAAAGCTATAATTTGTGAAGTTGCACCTAGAGATGGTTTTCAAGCAGAGCC
AGAGTGGATTCCAACAGACAAAAAAGTAGAAATGATTAGAAGTCTTGCTAAAACGGGTATTACTACAATGGAAGTGACTT
CATTCGTCCATCCTAAAGCAATTCCACAACTTAAGGATGCAGAGGAAGTTGTCAGTAGAGTACATGATATAGAAAATCTT
CATCTAAGAGCATTAGTCCCTAATTTAACGGGAGCGCAACGAGCTGTTAATGCTGGAGTGAAGAAATTAAAGCTAATGCT
CTCAGCCACCGATTCGCACAGTTTATCTAATGCGAATGCTACTGTAATAGAAGCACAAAATAAATTAGAACCAATTATTG
AATACGCCAACCAAAACGATACAAAAGTCGGCGGTTCCATCTCGGTTGCTTTTGGTTGTCCGTATGAAGGTAAAGTGGGA
GTAAATCAGTTAATTCCGATACTAGATCGTTACCAAAAGATGGGGATTGAAGAGGTTTCCTTAGCTGATACTACAGGCAT
GGCAACTCCTAAGCAAGTATATGAAACACTTGGGGTTTTAAAAGAGACATACCCAACCCTTACTTTCTCCATGCATTTTC
ATAATACAAGAGGAATGGCATTAGCTAATACGATAAGTGCACTCCAACAAGGAGTTATTCGTTTTGATAGTTCTATCGCT
GGGTTAGGCGGTTGTCCGTATGCACCAGGAGCATCAGGGAATGTTGCAACAGAGGATTTAGTGCATTTCCTACACGAAAA
TGGTGTAGATACTGGCATAAATCTTTCCTCTGTAATCACCACTGCTAAAGAAGTGAAAAACATCATCGGTCATGATGGAG
GGAGTTTTATACTGCAAGCAGGACCTTCCGCTGACCTTCATTCCAAGCCTAAATCTCAACAAAAAATAAATAATTAA

Upstream 100 bases:

>100_bases
GCCAGAATTAGGGGAACATAATGAAGAGATTCTTGGAAATCACCTAGGTCTTTCAGAAGCAGAAATTAGTACACTAAAGG
AACAGGGGGTAATCTAGTTT

Downstream 100 bases:

>100_bases
TTTTAAAATCATTCAAACCGCATGGAATATTCTGTGCGGTTTGAATGATTTCCTAGTTAGTTGTAGCCCTGCATGTGGAA
AAACTGTCAACAATTGAATT

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MADQAFLLPTKAIICEVAPRDGFQAEPEWIPTDKKVEMIRSLAKTGITTMEVTSFVHPKAIPQLKDAEEVVSRVHDIENL
HLRALVPNLTGAQRAVNAGVKKLKLMLSATDSHSLSNANATVIEAQNKLEPIIEYANQNDTKVGGSISVAFGCPYEGKVG
VNQLIPILDRYQKMGIEEVSLADTTGMATPKQVYETLGVLKETYPTLTFSMHFHNTRGMALANTISALQQGVIRFDSSIA
GLGGCPYAPGASGNVATEDLVHFLHENGVDTGINLSSVITTAKEVKNIIGHDGGSFILQAGPSADLHSKPKSQQKINN

Sequences:

>Translated_318_residues
MADQAFLLPTKAIICEVAPRDGFQAEPEWIPTDKKVEMIRSLAKTGITTMEVTSFVHPKAIPQLKDAEEVVSRVHDIENL
HLRALVPNLTGAQRAVNAGVKKLKLMLSATDSHSLSNANATVIEAQNKLEPIIEYANQNDTKVGGSISVAFGCPYEGKVG
VNQLIPILDRYQKMGIEEVSLADTTGMATPKQVYETLGVLKETYPTLTFSMHFHNTRGMALANTISALQQGVIRFDSSIA
GLGGCPYAPGASGNVATEDLVHFLHENGVDTGINLSSVITTAKEVKNIIGHDGGSFILQAGPSADLHSKPKSQQKINN
>Mature_317_residues
ADQAFLLPTKAIICEVAPRDGFQAEPEWIPTDKKVEMIRSLAKTGITTMEVTSFVHPKAIPQLKDAEEVVSRVHDIENLH
LRALVPNLTGAQRAVNAGVKKLKLMLSATDSHSLSNANATVIEAQNKLEPIIEYANQNDTKVGGSISVAFGCPYEGKVGV
NQLIPILDRYQKMGIEEVSLADTTGMATPKQVYETLGVLKETYPTLTFSMHFHNTRGMALANTISALQQGVIRFDSSIAG
LGGCPYAPGASGNVATEDLVHFLHENGVDTGINLSSVITTAKEVKNIIGHDGGSFILQAGPSADLHSKPKSQQKINN

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=293, Percent_Identity=39.2491467576792, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI109150427, Length=293, Percent_Identity=39.2491467576792, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI109150422, Length=293, Percent_Identity=39.2491467576792, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI260654708, Length=134, Percent_Identity=44.7761194029851, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17510563, Length=285, Percent_Identity=42.1052631578947, Blast_Score=237, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6320071, Length=227, Percent_Identity=27.7533039647577, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320019, Length=227, Percent_Identity=27.7533039647577, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24582381, Length=293, Percent_Identity=39.2491467576792, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 34153; Mature: 34022

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQAFLLPTKAIICEVAPRDGFQAEPEWIPTDKKVEMIRSLAKTGITTMEVTSFVHPKA
CCCCCEECCCHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCC
IPQLKDAEEVVSRVHDIENLHLRALVPNLTGAQRAVNAGVKKLKLMLSATDSHSLSNANA
CCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
TVIEAQNKLEPIIEYANQNDTKVGGSISVAFGCPYEGKVGVNQLIPILDRYQKMGIEEVS
EEEECHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEE
LADTTGMATPKQVYETLGVLKETYPTLTFSMHFHNTRGMALANTISALQQGVIRFDSSIA
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
GLGGCPYAPGASGNVATEDLVHFLHENGVDTGINLSSVITTAKEVKNIIGHDGGSFILQA
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEC
GPSADLHSKPKSQQKINN
CCCCCCCCCCCHHHCCCC
>Mature Secondary Structure 
ADQAFLLPTKAIICEVAPRDGFQAEPEWIPTDKKVEMIRSLAKTGITTMEVTSFVHPKA
CCCCEECCCHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCC
IPQLKDAEEVVSRVHDIENLHLRALVPNLTGAQRAVNAGVKKLKLMLSATDSHSLSNANA
CCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
TVIEAQNKLEPIIEYANQNDTKVGGSISVAFGCPYEGKVGVNQLIPILDRYQKMGIEEVS
EEEECHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEE
LADTTGMATPKQVYETLGVLKETYPTLTFSMHFHNTRGMALANTISALQQGVIRFDSSIA
CCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
GLGGCPYAPGASGNVATEDLVHFLHENGVDTGINLSSVITTAKEVKNIIGHDGGSFILQA
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEC
GPSADLHSKPKSQQKINN
CCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]