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The map label for this gene is ribH

Identifier: 23100668

GI number: 23100668

Start: 3346255

End: 3346740

Strand: Reverse

Name: ribH

Synonym: OB3213

Alternate gene names: 23100668

Gene position: 3346740-3346255 (Counterclockwise)

Preceding gene: 23100669

Following gene: 23100667

Centisome position: 92.18

GC content: 39.09

Gene sequence:

>486_bases
ATGACAATTTATGAAGGCAACTATAATGGACAGGGCAAGAAGATAGCGATTGTAACAAGTAGATTTAATGAATTTATTAC
TTCGAAACTATTATCTGGGGCAGAAGATATGCTTATTCGTCACGGCGTAGCTAGGGAAAATATTGATGTGATTTGGGTTC
CAGGTGCTTTTGAGATTCCTTATATCACCAAGCGCGCAGTCCATTCTCAAAAATACGACGGAGTAATAACGTTAGGAACA
GTGATTCGCGGAGCAACGACACATTATGATTATGTTTGTAATGAAGTCTCTAAGGGAGTAGCTCAAGTAGGGATGAATAG
TAACATACCTGTATTGTTTGGTGTGCTAACAACGGAATCGATTGAGCAAGCTGTAGAAAGAGCCGGTACGAAAGCTGGAA
ATAAAGGTGCGGAAACAGCACTCGGATTACTTGAAATGATATCATTAAACGAACAATTTCATGTAGAGAGTGTTGGAATG
AACTAA

Upstream 100 bases:

>100_bases
CAAAAGACTGGGATTTGACCTTGATTACAGTAGAAGATCTCGTTCAATATCGAGAAAAAGAAATAGCTATCGAAAATTAA
TCTTAAAGGAGTTTGATCAT

Downstream 100 bases:

>100_bases
TTTAAACATACTGTCCAGAAGTCTTCATCACGATTCACGAGAAGGCTATGGACAGTTTTTTTAATCCCCAGGGTATGCAT
CCATAACCTTGATGTCAAAA

Product: riboflavin synthase beta chain

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MTIYEGNYNGQGKKIAIVTSRFNEFITSKLLSGAEDMLIRHGVARENIDVIWVPGAFEIPYITKRAVHSQKYDGVITLGT
VIRGATTHYDYVCNEVSKGVAQVGMNSNIPVLFGVLTTESIEQAVERAGTKAGNKGAETALGLLEMISLNEQFHVESVGM
N

Sequences:

>Translated_161_residues
MTIYEGNYNGQGKKIAIVTSRFNEFITSKLLSGAEDMLIRHGVARENIDVIWVPGAFEIPYITKRAVHSQKYDGVITLGT
VIRGATTHYDYVCNEVSKGVAQVGMNSNIPVLFGVLTTESIEQAVERAGTKAGNKGAETALGLLEMISLNEQFHVESVGM
N
>Mature_160_residues
TIYEGNYNGQGKKIAIVTSRFNEFITSKLLSGAEDMLIRHGVARENIDVIWVPGAFEIPYITKRAVHSQKYDGVITLGTV
IRGATTHYDYVCNEVSKGVAQVGMNSNIPVLFGVLTTESIEQAVERAGTKAGNKGAETALGLLEMISLNEQFHVESVGMN

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=150, Percent_Identity=57.3333333333333, Blast_Score=166, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6324429, Length=148, Percent_Identity=32.4324324324324, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_OCEIH (Q8ELL1)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694135.1
- ProteinModelPortal:   Q8ELL1
- SMR:   Q8ELL1
- GeneID:   1016538
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3213
- NMPDR:   fig|221109.1.peg.3215
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- BioCyc:   OIHE221109:OB3213-MONOMER
- BRENDA:   2.5.1.9
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 17523; Mature: 17392

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIYEGNYNGQGKKIAIVTSRFNEFITSKLLSGAEDMLIRHGVARENIDVIWVPGAFEIP
CEEECCCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEECCCCCCC
YITKRAVHSQKYDGVITLGTVIRGATTHYDYVCNEVSKGVAQVGMNSNIPVLFGVLTTES
HHHHHHHHHHCCCCEEEECHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHH
IEQAVERAGTKAGNKGAETALGLLEMISLNEQFHVESVGMN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure 
TIYEGNYNGQGKKIAIVTSRFNEFITSKLLSGAEDMLIRHGVARENIDVIWVPGAFEIP
EEECCCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEECCCCCCC
YITKRAVHSQKYDGVITLGTVIRGATTHYDYVCNEVSKGVAQVGMNSNIPVLFGVLTTES
HHHHHHHHHHCCCCEEEECHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHH
IEQAVERAGTKAGNKGAETALGLLEMISLNEQFHVESVGMN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376